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The application belongs to the technical field of biological pesticides, and particularly relates to application of a bacteriostatic agent in inhibition of Fusarium fungi. The bacteriostatic agent contains at least one component of chromanol or tryptanthrin, and can effectively inhibit the growth of F. proliferatum, and the inhibition rate of F. proliferatum is as high as 100%. Since F. proliferatum is a dominant pathogenic fungus causing rhizome rot of Lanzhou lily, the bacteriostatic agent provided by the application can also be used for prevention and treatment of rhizome rot of lily, and the effective active component in the bacteriostatic agent is a metabolic product of microorganisms, so that the bacteriostatic agent has the advantages of green environmental protection, high efficiency and safety.