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Molecularly imprinted electrochemical sensor for detecting patulin and preparation method thereof

A technology of molecular imprinting and patulin, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of high sensitivity and good selectivity, cumbersome operation process, expensive instruments and equipment, etc., and achieve low price and simple preparation , the effect of large specific surface area

Active Publication Date: 2018-10-23
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, the detection methods of patulin mainly include gas phase / liquid chromatography, gas phase / liquid chromatography tandem mass spectrometry, etc. These detection methods have high sensitivity and a large amount of sample detection, and are also very suitable for the separation and analysis of complex samples; but Its instruments and equipment are expensive and the operation process is cumbersome, especially not conducive to the realization of on-site on-line detection
Although electrochemical detection can achieve fast on-line detection, and is easy to operate and low in analysis cost, it is difficult to achieve high sensitivity and good selectivity.

Method used

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  • Molecularly imprinted electrochemical sensor for detecting patulin and preparation method thereof
  • Molecularly imprinted electrochemical sensor for detecting patulin and preparation method thereof
  • Molecularly imprinted electrochemical sensor for detecting patulin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preparation of molecularly imprinted electrochemical sensors is mainly divided into the following three steps:

[0050] 1. The preparation of Th-PtNPs-NGEs (thionine-Pt nanoparticles-nitrogen-doped graphene) composite nanomaterials is synthesized in two steps, as follows:

[0051] Step 1: 4.5 mL of H 2 PtCl 6 ·6H 2 O aqueous solution (20mmol L -1 ) and 110 μL hydrochloric acid aqueous solution (6mol L -1 ) were mixed, 50 mg of nitrogen-doped graphene was dispersed in the mixture, and ultrasonically mixed. Then add 5.0 mL of ascorbic acid aqueous solution (0.1 mol L -1 ) as a reducing agent, at room temperature ultrasonic 4h, reduction reaction. After the reaction, the black reaction product was separated by centrifugation, washed three times with acetonitrile and distilled water, and dried at 50°C to prepare Pt nanoparticles-nitrogen-doped graphene composite nanomaterials, which were stored at room temperature for later use.

[0052] The second step: 5 mg of ...

Embodiment 2

[0062] Patulin Molecularly Imprinted Electrochemical Sensor for Determination of Patulin in Samples

[0063] All electrochemical experiments were carried out on a CHI 660 electrochemical analyzer (Shanghai Chenhua Instrument Co., Ltd.). The three-electrode system included: the molecularly imprinted electrochemical sensor prepared in Example 1 was used as a working electrode, and a saturated calomel electrode (SCE , produced by Jiangsu Electrochemical Instrument Factory) was used as a reference electrode, and a platinum wire electrode was used as a counter electrode.

[0064] All experiments were done at room temperature.

[0065] Tested by differential pulse voltammetry (DPV), the electrolyte is 0.1mol L -1 Phosphate buffer solution (PBS, pH 6.0), DPV scanning potential from 0.2V to -0.6V. Finally, the concentration of patulin in the solution to be tested was determined by calculating the standard curve between the logarithm of the concentration of patulin in the solution (l...

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Abstract

The invention provides a molecularly imprinted electrochemical sensor for detecting patulin. The sensor comprises a glassy carbon electrode, a thionine-Pt nanoparticle-nitrogen-doped graphene composite nanomaterial layer and a molecularly imprinted polymer layer. The molecularly imprinted electrochemical sensor can effectively amplify the signal, has high sensitivity and has good selectivity, a high recovery rate and good reproducibility. The molecularly imprinted electrochemical sensor is free of expensive large instruments, has a simple pretreatment process, ensures the accuracy of the results, realizes fast detection, greatly saves a cost and improves detection efficiency.

Description

technical field [0001] The invention relates to the technical field of molecularly imprinted electrochemical sensors, in particular to a molecularly imprinted electrochemical sensor for detecting patulin and a preparation method thereof. Background technique [0002] Patulin (PAT), also known as patulin, 4-hydroxy-4-hydro-furan (3,2C) pyran-2 (6H) ketone (4-hydroxy-4H-furo [3,2C] pyran-2(6H)-one), the molecular formula is C 7 h 6 o 4 , is a neurotoxic fungal secondary metabolite with potential teratogenicity, carcinogenicity and mutagenicity. Patulin widely exists in fruits and their products, causing serious impact on human health and economy. International organizations and many countries have established the maximum limit of patulin, and my country has also stipulated that the limit of patulin in apples, hawthorns and their products should not exceed 50 μg kg -1 . Therefore, the detection and analysis method of patulin is very important and has become an internation...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 韩铮聂冬霞赵志辉蒋可秋黄晴雯郭文博孟佳佳
Owner SHANGHAI ACAD OF AGRI SCI
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