Application of encapsulated cryptococcus laurentii to removal of patulin

A technology of Cryptococcus lorensis and patulin, which is applied to the application field of encapsulation treatment of Cryptococcus lorens in removing patulin, and can solve the problems of difficult removal of yeast and the like

Inactive Publication Date: 2016-04-20
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, it has been found that Cryptococcus laurentis also has the ability to degrade patulin, but how to apply its ability is a difficult problem

Method used

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  • Application of encapsulated cryptococcus laurentii to removal of patulin
  • Application of encapsulated cryptococcus laurentii to removal of patulin

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1, the method for encapsulating Cryptococcus laurentis to remove patulin

[0040] 1. Encapsulation of Cryptococcus laurentis

[0041] 1. Preparation of yeast cell suspension

[0042] Take the Cryptococcus laurentii strain frozen in the -80°C refrigerator, and streak it on the NYDA plate. After the colony grows, pick a single colony into the NYDB, 28°C, 220rpm / min, and cultivate for 18h. After counting, the final concentration of cells was 1×10 5 cells / ml were transferred to 40ml NYDB, cultured at 28°C, 220rpm / min for 48h to obtain yeast cell suspension;

[0043] 2. Encapsulation treatment

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Abstract

The invention discloses application of encapsulated cryptococcus laurentii to removal of patulin, and provides a method for removing patulin in a system by use of cryptococcus laurentii. The method comprises the following steps: 1) mixing cryptococcus laurentii or a suspension thereof, a sodium alginate water solution and a CaCl2 water solution to obtain cryptococcus laurentii colloidal particles; 2) uniformly mixing the cryptococcus laurentii colloidal particles with a system containing patulin and reacting so as to remove the patulin in the system. The application shows that the encapsulated cryptococcus laurentii can be used for removing the patulin in the system, and the encapsulated cryptococcus laurentii can be recovered without affecting the system.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of capsule processing Cryptococcus laurentis in removing patulin. Background technique [0002] Patulin is a secondary metabolite of some Penicillium and Aspergillus fungi. It has extensive and strong toxic effects on humans and animals. Acute toxicity manifests as convulsions, convulsions, subcutaneous tissue edema, pulmonary and intestinal congestion, enteritis, anuria, and death; chronic toxicity can lead to gastric ulcers, immunosuppression, teratogenicity, and potential carcinogenicity, etc., and may also affect men of fertility. Patulin is soluble in water, ethanol, acetone, ethyl acetate and chloroform, slightly soluble in ether and benzene, insoluble in petroleum ether, and chemically stable under acidic conditions. [0003] Among the fungi capable of producing patulin, some fungi are plant pathogens, and the most important one is Penicillium expansum. Thi...

Claims

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Application Information

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IPC IPC(8): A23L5/20
Inventor 田世平李博强秦国政张占全
Owner INST OF BOTANY CHINESE ACAD OF SCI
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