Application of pseudomonas aeruginosa to patulin degradation
A technology of Pseudomonas aeruginosa and patulin, applied in the field of microorganisms, can solve the problems that have not been seen in the research and reports of Pseudomonas aeruginosa degrading patulin, and achieve the effect of good application prospects
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[0024] Embodiment 1, the degradation effect of Pseudomonas aeruginosa N17-1 to patulin
[0025] NB liquid medium: composed of solvent and solute; the solute is peptone, beef extract and NaCl, the solvent is water; the concentration of peptone in NB liquid medium is 1g / 100ml, and the concentration of beef extract in NB liquid medium is 0.3 g / 100ml, the concentration of NaCl in NB liquid medium is 0.5g / 100ml.
[0026] 1. Inoculate Pseudomonas aeruginosa N17-1 in liquid NB medium to initial OD 600nm =0.05, under the condition of 37° C., shake culture at 200 rpm (rotation radius: 20 mm) for 24 hours, and collect all the bacterial liquid (that is, the entire culture system including bacterial cells and supernatant).
[0027] 2. Dissolve 1 mg of patulin standard product in 10 ml of chromatographically pure methanol to obtain a patulin solution with a concentration of 100 ppm.
[0028] 3. Prepare the experimental group solution:
[0029] After the bacterial suspension is fully mix...
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