Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for easily and conveniently detecting patulin

A simple and convenient technique for patulatoxin, applied in the field of analytical chemistry, which can solve the problems of need, complicated method and high detection cost

Inactive Publication Date: 2016-04-20
QINGDAO UNIV OF SCI & TECH
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have their own advantages and can meet the detection requirements of patulin to varying degrees, but the methods are complicated, expensive instruments are required, and the detection cost is high.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for easily and conveniently detecting patulin
  • Method for easily and conveniently detecting patulin
  • Method for easily and conveniently detecting patulin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Effect of Reaction Time of Aptamer and Patulin on Signal

[0028] In this detection method, the action time of patulin and its aptamer directly affects the detection signal intensity of the blood glucose meter. figure 2 It reflects the influence of different reaction times on the readings of the blood glucose meter. It can be seen from the figure that the maximum detection signal appears at 50 minutes of reaction, and then with the increase of reaction time, the detection signal decreases. Therefore, 50 minutes is selected as the patulin toxin Optimal reaction time for reaction with its aptamer.

Embodiment 2

[0029] Embodiment 2 specificity evaluation

[0030] According to the above operation process, the concentration of 1.0ng / mL patulin (a) and the concentration of 100.0ng / mL ochratoxin (b), Staphylococcus aureus enterotoxin C1 (c), versicolor ( d) and the response of trichothecenes (e) to the sensor, the blood glucose meter reading results are as follows Figure 4As shown, the results showed that 100-fold ochratoxin, Staphylococcus aureus enterotoxin C1, versicolor and trichothecenes did not interfere with the determination of patulin at a concentration of 1.0 ng / mL, indicating that the established The method has good selectivity to patulin.

Embodiment 3

[0031] The determination of patulin toxin in embodiment 3 milk and water sample

[0032] In order to verify the feasibility of the method, this method was used to determine the content of patulin in complex samples, and the recovery rates measured by adding different concentrations of patulin to milk and water samples are shown in Table 1. The recoveries were between 98.1% and 101.3%, which indicated the feasibility of the method for determining actual samples.

[0033] Table 1 Recovery test

[0034]

[0035] a Averageoffivedeterminations.

[0036]

[0037]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of analytical chemistry, and particularly relates to a method for easily and conveniently detecting patulin. The method comprises the steps that firstly, a patulin aptamer is fixed in a 96-pore plate micropore, an invertase modified aptamer complementary sequence serves as a probe, and the probe is fixed in the 96-pore plate micropore through DNA hybridization. When the target object patulin exists, recognition action is conducted between the patulin and the patulin aptamer, disengagement of the invertase modified aptamer complementary sequence probe is achieved, a disengaged probe solution is taken to be put into a small centrifugal tube, saccharose is added and converted into glucose under the action of invertase on the probe, a glucometer is utilized to serve as a detecting instrument, and determination of the patulin is achieved.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for conveniently detecting patulin toxin. Background technique [0002] The development of convenient and inexpensive methods for the determination of foodborne pathogens is of great significance for food safety. It has been reported that some non-glucose targets can be measured using a home glucose meter. Patulin is a fungal metabolic toxin produced by the genus Penicillium, Aspergillus, and Filamentosa. It has a series of acute toxicity and chronic toxicity such as teratogenicity and carcinogenicity to the human body. Studies have shown that patulin has acute toxicity such as causing animal convulsions, lung and liver hemorrhage, kidney damage, and intestinal hemorrhage, and has chronic toxicity such as neurotoxicity, immunotoxicity, genotoxicity, teratogenicity, and carcinogenicity [MoakeMM, Padilla- Zakour OI, Worobo RW. Comprehensive review of patul...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/50
CPCG01N33/50
Inventor 混旭钟华王周平
Owner QINGDAO UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com