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Method for controlling apple patulin

A technology for patulin and apples, which is applied in the field of controlling apple patulin, and achieves significant economic and social benefits, simple method of use, and low cost

Active Publication Date: 2011-09-28
东台海滨科技创业园管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the combined use of phytic acid and antagonistic yeast to control apple patulin at home and abroad.

Method used

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  • Method for controlling apple patulin
  • Method for controlling apple patulin
  • Method for controlling apple patulin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the control of phytic acid combined with Rhodotorula gelatin to apple wound patulin

[0026] 1. Test plan

[0027] After fruit treatment, wounds of uniform size and depth were formed on the equator of each fruit surface with a sterilized puncher, and 30 μl of the following treatment solution was added to each wound in equal amounts: (1) 1×10 8 cells / ml of yeast (containing 0.2 % PA); (2) 1×10 8 cells / ml yeast; (3) PA (0.2%); (4) sterile water. After treatment, the fruits were placed in plastic baskets, sealed with plastic wrap, and stored at 25°C. After 3 hours, add an equal amount of 30 μl 5 × 10 4 spores / ml P. expansum spore suspension. After natural drying, the fruits were put into plastic baskets and sealed with plastic wrap, and cultivated in a constant temperature and humidity incubator (25°C, RH 95%). Each treatment was replicated 3 times, and each replicated 12 fruits.

[0028] Sample extraction and purification: apples were used as sample...

Embodiment 2

[0032] Embodiment 2: Phytic acid binding Rhodotorula colloides of different concentrations is to Penicillium expansum ( P. expansum ) inhibitory effect on mycelium growth

[0033] 1. Test plan

[0034] A small hole with a diameter of 10 mm was punched in a 9 cm Petri dish containing 30 mL of PDA medium, and 1 × 10 8 Cells / ml Rhodotorula colloides suspension (containing 0, 0.1%, 0.2%, 0.3%, 0.4%, 0.5% phytic acid) and 100 μl of sterile water were placed in the well-made holes. After 3 hours, the injection concentration was 5×10 4 spores / ml Penicillium extensa ( P. expansum ) 100 μl. Three plates were made for each treatment. After 1 hour, they were sealed with polyethylene (PE) plastic wrap to prevent cross-infection, and placed in a constant temperature and humidity incubator (RH 95%) at 28°C for cultivation. After 7 days, each plate was measured with a vernier caliper. The diameter of the mold lesions was measured, and the test was repeated three times to observe the ...

Embodiment 3

[0038] Embodiment 3: Phytic acid binding Rhodotorula colloides to the degradation of patulin

[0039] 1. Test plan

[0040] Put 50ml of NYDB (beef extract 8g, yeast extract 5g, glucose 10g, water 1000ml) seed medium into a 250ml triangular flask, and use the inoculation loop to connect the two loops of activated R. mucilaginosa , cultured at 200rpm, 28°C for 20h; centrifuge the above culture mixture at 7000×g for 10min, wash twice with sterile normal saline, remove the culture medium, resuspend the yeast cells with sterile normal saline, and place on a hemocytometer Adjust the cell concentration to 5 x 10 8 cells / ml, put 50ml NYDB medium in a 250ml Erlenmeyer flask, add 1ml of yeast cell culture solution of the above concentration (phytic acid content is 0, 0.2%), and add patulin standard substance with a concentration of 1mg at the same time, and then in Cultivate at 200rpm and 28°C for 24h; centrifuge the above-mentioned yeast culture mixture at 7000×g for 10-15min, filte...

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Abstract

The invention discloses a method for controlling apple patulin, belonging to a method for controlling plant postharvest diseases. The method provided by the invention comprises the following steps of: activating rhodotorula mucilaginosa, inoculating the rhodotorula mucilaginosa into an NYDB (Nutrient Yeast Dextrose Broth) culture medium to be cultured and centrifuging to obtain thalli; diluting the thalli by utilizing a 0.2% phytic acid solution to prepare a thalli suspension solution with the concentration of 1*10 to the eighth power per milliliter; adding 30 milliliters of microzyme suspension solution into the wounds of the fruit and naturally drying; and then putting the fruit into a plastic basket and sealing the plastic basket by a preservative film. In the method disclosed by the invention, the phytic acid and the rhodotorula mucilaginosa are combined to control the apple patulin; and the method is environmental-friendly, safe, economical, efficient and practical and has important social values and economic values.

Description

technical field [0001] The present invention relates to the use of phytic acid (phytic acid) and Rhodotorula glucosa ( Rhodotorula mucilaginosa ) combined with the method of controlling apple patulin, which belongs to the method of controlling postharvest plant diseases. Background technique [0002] Patulin, also known as patulin, is a poisonous fungal metabolite produced by the metabolism of Penicillium, Aspergillus, and silkworms. It mainly exists in moldy and rotten fruits and products. One of the main factors of quality. Patulin is a worldwide fruit pollutant, which is found in many kinds of fruits, fruit products and fruit wine, especially apples and their processed products. The pollution of patulin is quite serious. The limit standard of patulin in most European and American countries is 0 – 50 μg / kg; WHO recommends that the maximum limit standard of patulin in apple juice is 50 μg / kg; the corresponding standard in my country stipulates that the limit standard of a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154
Inventor 张红印赵利娜任晓锋张晓云张海晖朱淑云
Owner 东台海滨科技创业园管理有限公司
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