Method for improving efficacy of cryptococcus laurentii with control on penicilliosis and patulin of post-picked apples

A technology of Cryptococcus laurentis and patulin, which is applied in the field of biological control, can solve the problems that the biological control effect cannot meet the required requirements and is unstable, and achieve the goal of avoiding harm to human health, low cost and convenient operation Effect

Inactive Publication Date: 2012-10-03
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the uncertainty of environmental conditions, under commercial production conditions, biological antagonistic bacteria are used as live bacteria preservatives, and their biological control effects either fail to meet the required requirements or are unstable. It is difficult to achieve ideal results by using yeast antagonistic bacteria alone to prevent postharvest diseases. Effect

Method used

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  • Method for improving efficacy of cryptococcus laurentii with control on penicilliosis and patulin of post-picked apples
  • Method for improving efficacy of cryptococcus laurentii with control on penicilliosis and patulin of post-picked apples
  • Method for improving efficacy of cryptococcus laurentii with control on penicilliosis and patulin of post-picked apples

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 2: Trehalose-induced culture of Cryptococcus laurentis for the control of apple penicillium

Embodiment 2

[0027] 1. Test plan

[0028] After the apples were processed, a 5 mm (diameter) x 3 mm (depth) wound of uniform size and depth was formed on the equator of each fruit surface with a sterilized punch. Add 30 μl (1) Cryptococcus laurentis suspension cultured in NYDB medium (concentration: 5×10 8 cells / ml); (2) Yeast suspension cultured in trehalose-supplemented medium (carbon source is trehalose and glucose), the concentration of trehalose is 0.2%, 0.4%, 0.6%, 0.8%, 1.0% (NYTB culture medium, all glucose was replaced with trehalose); (3) sterile water (as a control). After 3 hours, add 30 μl in equal amount to each wound P. expansum Spore suspension (concentration of 5 × 10 4 spores / ml). After drying naturally, put the apples into plastic baskets, seal them with plastic wrap to maintain the humidity in the baskets, and store them in a constant temperature and humidity incubator at 20°C with a relative humidity of 95%RH. Each treatment was replicated 3 times, each replica...

Embodiment 3

[0034] 1. Test plan

[0035] After the apples were processed, a 5 mm (diameter) x 3 mm (depth) wound of uniform size and depth was formed on the equator of each fruit surface with a sterilized punch. Add 30 μl (1) Cryptococcus laurentis suspension cultured in NYDB medium (concentration: 5×10 8 cells / ml); (2) Yeast suspension cultured in trehalose-supplemented medium (carbon source is trehalose and glucose), the concentration of trehalose is 0.2%, 0.4%, 0.6%, 0.8%, 1.0% (NYTB culture medium, all glucose was replaced with trehalose); (3) sterile water (as a control). After 3 hours, add 30 μl in equal amount to each wound P. expansum Spore suspension (concentration of 5 × 10 4 spores / ml). After drying naturally, put the apples into plastic baskets, seal them with plastic wrap to maintain the humidity in the baskets, and store them in a constant temperature and humidity incubator at 20°C with a relative humidity of 95%RH. Each treatment was replicated 3 times, each replica...

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Abstract

The invention discloses a method for improving the efficacy of cryptococcus laurentii with control on the penicilliosis and the patulin of post-picked apples, belonging to the technical field of biological control. The method is carried out according to following steps: activating the cryptococcus laurentii, inoculating the cryptococcus laurentii to a trehalose supplemental medium (a carbon source comprises 0.4% of trehalose and 0.6% of glucose) for culturing, and centrifuging the cryptococcus laurentii to obtain thalli; preparing the thalli into a 1*108 pieces/ml bacterium suspension; and adding 30mul yeast bacterium suspensions to the wounds of fruit, naturally drying the fruit, putting the fruit into a plastic basket, sealing by plastic wraps, and storing the fruit at a room temperature. Using the trehalose to improve the efficacy of the cryptococcus laurentii with control on the penicilliosis and the patulin of the post-picked apples, the method for improving the efficacy of the cryptococcus laurentii with control on the penicilliosis and the patulin of the post-picked apples is friendly to environment, safe, economical, efficient and applicable, thereby having important social values and economic values.

Description

technical field [0001] The invention belongs to the technical field of biological control, and relates to a Cryptococcus laurentii ( Cryptococcus laurentii ) and a method for using trehalose to improve the effectiveness of Cryptococcus laurentis in controlling apple postharvest penicillium and patulin. Background technique [0002] Patulin, also known as patulin, is a toxic fungal secondary metabolite produced by the metabolism of Penicillium, Aspergillus, and Clothyma, and is a genotoxic compound with potential cell and animal toxicity , has teratogenicity, carcinogenicity and immunotoxicity, and is one of the most harmful mycotoxins to humans. Patulin is a kind of fruit pollutant worldwide. The contamination of patulin in food production is quite common. It is widely found in apples, grapes, oranges, pineapples, corn, cheese and cereals, especially in apples and apples. The pollution of patulin is the most serious in the products that are processed raw materials. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/155
Inventor 张红印靳莎莎任晓锋张晓云李超兰
Owner JIANGSU UNIV
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