Method for improving efficacy of cryptococcus laurentii with control on penicilliosis and patulin of post-picked apples
A technology of Cryptococcus laurentis and patulin, which is applied in the field of biological control, can solve the problems that the biological control effect cannot meet the required requirements and is unstable, and achieve the goal of avoiding harm to human health, low cost and convenient operation Effect
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Embodiment 1
[0026] Example 2: Trehalose-induced culture of Cryptococcus laurentis for the control of apple penicillium
Embodiment 2
[0027] 1. Test plan
[0028] After the apples were processed, a 5 mm (diameter) x 3 mm (depth) wound of uniform size and depth was formed on the equator of each fruit surface with a sterilized punch. Add 30 μl (1) Cryptococcus laurentis suspension cultured in NYDB medium (concentration: 5×10 8 cells / ml); (2) Yeast suspension cultured in trehalose-supplemented medium (carbon source is trehalose and glucose), the concentration of trehalose is 0.2%, 0.4%, 0.6%, 0.8%, 1.0% (NYTB culture medium, all glucose was replaced with trehalose); (3) sterile water (as a control). After 3 hours, add 30 μl in equal amount to each wound P. expansum Spore suspension (concentration of 5 × 10 4 spores / ml). After drying naturally, put the apples into plastic baskets, seal them with plastic wrap to maintain the humidity in the baskets, and store them in a constant temperature and humidity incubator at 20°C with a relative humidity of 95%RH. Each treatment was replicated 3 times, each replica...
Embodiment 3
[0034] 1. Test plan
[0035] After the apples were processed, a 5 mm (diameter) x 3 mm (depth) wound of uniform size and depth was formed on the equator of each fruit surface with a sterilized punch. Add 30 μl (1) Cryptococcus laurentis suspension cultured in NYDB medium (concentration: 5×10 8 cells / ml); (2) Yeast suspension cultured in trehalose-supplemented medium (carbon source is trehalose and glucose), the concentration of trehalose is 0.2%, 0.4%, 0.6%, 0.8%, 1.0% (NYTB culture medium, all glucose was replaced with trehalose); (3) sterile water (as a control). After 3 hours, add 30 μl in equal amount to each wound P. expansum Spore suspension (concentration of 5 × 10 4 spores / ml). After drying naturally, put the apples into plastic baskets, seal them with plastic wrap to maintain the humidity in the baskets, and store them in a constant temperature and humidity incubator at 20°C with a relative humidity of 95%RH. Each treatment was replicated 3 times, each replica...
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