221 results about "Fungal Toxins" patented technology

A feed nano additive for adsorbing fungal toxin in feed is prepared through proportionally mixing montmorillonite, sodium chloride and water, stirring, water washing, preparing pulp again; adding glucomannan while high-speed stirring, reaction, ageing, water washing, dewatering; drying filter residue and breaking. Its advantages are broad-spectrum adsorption of different toxins, high adsorptive power, no influence of pH value, low residual and low dosage (0.02-0.2%).

The invention relates to a preparation method and use of a CdS-Fe3O4-based electrochemiluminescence sensor and belongs to the field of electrochemiluminescence sensors. A CdS quantum dot is used as an electrochemiluminescence signal source, an antibody is effectively immobilized on the surface of a CdS-Fe3O4 nanometer compound by excellent biocompatibility and a large specific surface area of mesoporous ferroferric oxide, and the CdS quantum dot is immobilized on the surface of the mesoporous ferroferric oxide by a covalent bond so that an antibody capture substrate is obtained. The CdS-Fe3O4-based electrochemiluminescence sensor can realize fungaltoxin and hormone detection according to different electrochemiluminescence signal intensity values of different concentrations of objects to be detected.

The invention discloses an acinetobacter strain and application thereof to degradation of zearalenone. The acinetobacter strain disclosed by the invention is Acinetobacter sp. SM04, and is preserved in the China General Microbiological Culture Collection Center on December 5th, 2011, and the preservation number is CGMCC No. 5524. The Acinetobacter sp. SM04 disclosed by the invention has stronger degradation capability towards mycotoxins ZEN, can degrade more than 98% of zearalenone into low-estrogenic-activity product within 36 hours, cannot produce high-estrogenic-activity analogues such as zearalenone and zeranol, and has real detoxification capability. The acinetobacter strain can be applied to the treatment of corn grains, corn alcohol residues or other mycotoxin contaminated feed, so that safe food and animal feed are obtained, and further, a zearalenone degradation strain which has low cost and high efficiency and avoids secondary pollution is provided for the environment-friendly processing and treatment of the grains and feed.

The invention discloses a detection method for 16 kinds of fungaltoxin. The detection method comprises the following steps of extracting: weighing an accurate sample, putting into a centrifugal pipe,adding an extracting solution of an acetonitrile-water-acetic acid mixed solution, extracting in a vortex or oscillating mode, then centrifuging, sucking liquid supernatant into the centrifugal pipe,adding water into the centrifugal pipe, evenly mixing in a vortex mode, centrifuging, making the liquid supernatant pass through a polytetrafluoroethylene filter membrane and collecting to obtain filtrate A; adding stable isotopes: respectively and accurately sucking the filtrate A and a mixed standard solution of the 16 kinds of fungaltoxin into different internal insertion pipes, adding a mixedwork solution of 14 kinds of fungaltoxin stable isotopes and evenly mixing in a vortex mode. According to the detection method disclosed by the invention, a liquid chromatogram-tandem mass spectrometer is utilized for detection, instrument flexibility is remarkably improved, and the detection can be performed through a direct sample diluting and feeding mode; thus, target loss in a purification process is effectively avoided, dependence on a purifying material is reduced, detection cost is reduced, the sample pretreating efficiency is improved, and quickly detecting multiple components at thesame time is achieved.

The invention relates to a preparation method and application of a mycotoxin and hormone electrogenerated chemiluminescence sensor constructed based on NPCo/Co3O4-Au/RuSi@Ru(bpy)3<2+>, and belongs to the field of electrogenerated chemiluminescence sensors. RuSi@Ru(bpy)3<2+> is taken as an electrogenerated chemiluminescence signal source, an antibody is effectively supported to the surface of a nanometer composite NPCo/Co3O4-Au/RuSi@Ru(bpy)3<2+> by virtue of high biocompatibility and large specific surface area of a nanometer porous material NPCo/Co3O4-Au, and RuSi@Ru(bpy)3<2+> is supported to the surface of NPCo/Co3O4-Au to prepare an antibody capturing substrate through a covalent bond. Mycotoxins are detected according to different intensities of electrogenerated chemiluminescence signals of objects to be tested at different concentrations.

The invention provides a method for determining 16 mycotoxins in tea leaves by ultra-high performance liquid chromatography-tandem mass spectrometry, and belongs to the technical field of analytical chemistry. The method comprises: extracting a sample by formic acid/acetonitrile (10: 90); adding a QuEChERS salt packet, shaking and centrifuging; treating an extracting solution through an OASIS PRIME HLB small column and a Dspe purification pipe, separating by using a Waters HSS T3 chromatographic column, adopting a multi-reaction ion monitoring (MRM) mode of simultaneously scanning positive ions and negative ions, matching a tea substrate with a standard solution, and quantifying by using an isotope internal standard method. The method is stable, accurate, sensitive and rapid, and can meetthe requirements of analyzing various toxin residues in various tea leaves.

The invention relates to the field of biotechnology, in particular to an ochratoxin degrading enzyme, a coding gene, a recombinant vector, a cell, an additive and application thereof. The invention more specifically relates to the ochratoxin degrading enzyme with a sequence shown as SEQ ID NO:2 or a mutant thereof, a coding gene of the enzyme, a vector and a cell containing the coding gene, and anadditive containing the enzyme and/or cell and/or a fermentation product thereof, application of the enzyme, the coding gene, the vector, the cell or additive in degradation of ochratoxin and/or other fungal toxins, as well as a method for degradation of ochratoxin/or other fungal toxins. The ochratoxin degrading enzyme provided by the invention is environment-friendly, can efficiently degrade ochratoxin in a short time, and does not generate any harmful by-product. The enzyme can tolerate high temperature catalysis up to 80DEG C, also has low pH value requirement, has good stability, and achieve degradation of fumonisins and T2 toxin to a certain degree.

The invention relates to a matrix solid-phase dispersing agent and application thereof and in particular relates to a solid-phase dispersing agent taking a graphene oxide mixed material as a matrix and an application of the solid-phase dispersing agent, belonging to the technical field of chemistry. A mixed material of graphene oxide and Cleanert Florisil is utilized as a matrix solid-phase dispersing agent, and 18 fungal toxins in a wheat meal sample are measured through a positive ion mode MRM detection mode of high performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS). The matrix solid-phase dispersing agent has the advantages that the recovery rate of a fungal toxin detection method is improved, and the universality of the method is enhanced, so that the detection requirement can be met through a mass spectrum without a positive and negative ion switching mode, and the sensitivity and detection limit can meet the requirements of various standards.

The invention relates to a mushroom polysaccharide composite, which comprises mushroom culture solution and additive. The composite can improve nutrient value, prolong storage life or adsorb and remove harmful substance, such as mycotoxin, heavy metal, pesticide and caffeine.

The invention relates to the technical field of detection of fungal toxins and particularly relates to a method for simultaneously detecting various fungal toxins. The method comprises the following steps: adding samples into an extracting agent for oscillating centrifugation, so as to obtain residues and supernate of the samples; adding methanol and pure water into the residues and taking supernate of the residues; mixing the supernate of the samples and the supernate of the residues to obtain supernate; mixing the supernate and diluent and filtering, so as to obtain extracting solution; enabling the extracting solution to slowly go through a complex immunoaffinity column, draining the extracting solution, performing gradient elution at least twice and collecting eluent; drying the eluent with by blowing nitrogen, so as to obtain to-be-detected solution after constant volume; simultaneously detecting the content of fungal toxins in the to-be-detected solution with LC-MS (liquid chromatography-mass spectrometry), respectively drawing a standard curve of each fungal toxin and finally calculating the content of the fungal toxins in the samples. According to the method, the defect that only one single fungal toxin can be detected with the conventional fungal toxin detection method is overcome, the detection cost is low, the detection efficiency is improved, and the loss and the pollution of a reagent are reduced.

The invention discloses a method for simultaneously detecting seven fungal toxins in original auxiliary materials of baijiu. According to the method, an improved QuEChERS method is adopted for extraction, further purification is not needed, high performance liquid chromatography is utilized for achieving separation of the fungal toxins, combined quadrupole-electrostatic field orbitrap mass spectrometry (Q-Orbitrab) is achieved, efficient parent ion selectivity and high-resolution accurate molecular weight (The mass number can be accurate to five digits after the decimal point.) are combined, accurate qualitativeness and quantification of the fungal toxins in baijiu and the original auxiliary materials can be achieved, and quantification is conducted through a matrix external standard method. The fungal toxins can be completely and effectively separated within 9 min, the linear relation of the fungal toxins within separate linear response ranges is good, the correlation coefficient R<2> is larger than 0.999, the lowest limit of quantification is lower than 1.0 microgram/L, the lowest detection limit is lower than 0.1 microgram/L, the recovery rate ranges from 70% to 120%, and the relative standard deviation (RSD) ranges from 0.5% to 4%. Compared with the national standard method, the method has the advantages of being rapid, simple, convenient, economical, efficient, safe and the like and can be suitable for separation and quantitative determination of various fungal toxins in baijiu and original auxiliary material samples.

The invention discloses a method for detecting mycotoxin in fermented tea. The method comprises dissolving a fermented tea sample in a polar solution, carrying out salting-out, carrying out centrifugation, carrying out filtration, putting the filtrate in 1290/6460 ultra-high performance liquid chromatography-triple quadrupole mass spectrometer, carrying out analysis through a mobile phase (0.1% formic acid-5 mmol/L ammonium acetate-water)-methanol, wherein the mobile phase A is 0.1% formic acid-5 mmol/L ammonium acetate-water and the mobile phase B is methanol, and carrying out separation through a gradient elution method comprising improving a concentration of the mobile phase B step by step so that the mycotoxin in the fermented tea sample reaches to the base line in short time and then is separated. The method realizes effective extraction and determination of various mycotoxins in fermented tea, can detect a variety of mycotoxins at the same time and has the characteristics of high detection efficiency, low environmental pollution, simple operation and promotion and use easiness.

The invention discloses a zearalenone (ZEN) toxin degrading enzyme for acinetobacter and a coding gene and applications of the ZEN toxin degrading enzyme. The amino acid sequence of the ZEN toxin degrading enzyme for the acinetobacter is shown by SEQ ID NO.6. The sequence of the coding gene of the ZEN toxin degrading enzyme for the acinetobacter is shown by SEQ ID NO.5. The ZEN toxin degrading enzyme for the acinetobacter performs stronger degrading capacity on mycotoxin ZEN, is capable of degrading at least 90 percent of ZEN into a low-estrogen active product in 12h without generating high-estrogen active analogues, such as ZEN, zeranol and the like, and has a real detoxification effect. According to the invention, the coding sequence of the ZEN toxin degrading enzyme for acinetobacter sp.SM04 is determined, i.e. a Prx gene, and thereby, a foundation is laid for researching the active site of the enzyme and changing the Prx enzyme activity through site-specific mutagenesis in the future.

The invention belongs to the field of micromolecular detection, and relates to a method and a kit for fluorescence detection of micromolecular mycotoxin based on a metal organic framework and upconversion nanoparticles. The method comprises the following steps: S1, obtaining an up-conversion nanoparticle probe modified with a micromolecular mycotoxin aptamer; S2, carrying out synthesis and activation of MIL-101 (Cr); S3, combining the micromolecular mycotoxin with the aptamer; and S4, carrying out signal detection: detecting a fluorescence signal of a product obtained by the reaction in the step S3 by adopting a fluorospectro photometer. The upconversion nanoparticles doped with rare earth are adopted, the fluorescence intensity is stable, the background value is low, and compared with other methods, the fluorescence detection kit does not need complex operation steps, is high in applicability, high in detection result sensitivity and good in specificity, and can be applied to rapid detection of on-site samples.

The invention relates to an irradiation degradation processing method of fungaltoxin. The method comprises the following steps: (1) a fungaltoxin standard substance is prepared into a standard solution; the standard solution is subjected to the irradiation processing with the irradiation range of 0-200 kGy; the content of the fungaltoxin is measured through a liquid chromatogram-tandem mass spectrometry; (2) a toxic sample is subjected to the preprocessing after the irradiation with the irradiation range of 0-9 kGy; the degradation effect of the fungaltoxin is measured through the liquid chromatogram-tandem mass spectrometry; (3) the fungaltoxin standard solution with the highest concentration is selected as a sample; after the selected sample is subjected to the irradiation with the irradiation range of 0-200 kGy, a degradation product is subjected to detection analysis through the liquid chromatogram-tandem mass spectrometry. Through the adoption of the irradiation degradation processing method of the fungaltoxin, the research and environmental protection application of the fungaltoxin degradation product lacking the irradiation degradation are solved, good degradation effect is achieved, and the environmental-friendly detoxification processing for mildewed agricultural product wastes is achieved.

The invention relates to a method for detecting 9 kinds of mycotoxins in a cassia seed medicinal material. The method comprises the following steps: a step 1: preparation of a standard substance stocksolution; a step 2: preparation of a mixed standard substance stock solution; a step 3: preparation of a standard working solution; a step 4: preparation of a tested object solution; a step 5: preparation of a substrate matching standard solution; a step 6: determination.

The invention discloses a hybrid material graphene/C3N4 for photocatalytically degrading fungaltoxin. The hybrid material graphene/C3N4 with a layer-layer-layer assembly structure is prepared from graphene oxide and a nanometer photocatalyst g-C3N4 according to the mass ratio being (0.1-10):100 through a hydro-thermal synthesis method. In addition, the influence of the hybrid material graphene/C3N4 on photocatalytic degradation of the fungaltoxin is inspected through the conditions of the graphene modifying amount, photodegradation time and the like. The high-activity hybrid material graphene/C3N4 with visible-light responses is prepared through the hydrothermal method, the process is simple, the hybrid material graphene/C3N4 is suitable for industrial volume production, the photocatalytic degradation technology is applied to the field of fungaltoxin degrading, and high application prospects and practical value are achieved.

The invention relates to the technical field of analysis and detection, and in particular, relates to a detection kit for multiple fungaltoxins, and a preparation method, a detection method and application of the detection kit. The kit comprises up-conversion nanoparticles respectively coupled with antigens of fungaltoxins to be detected, magnetic nanoparticles respectively coupled with antibodies of the fungaltoxins to be detected, and a standard substance solution of the fungaltoxins, and the fungaltoxins to be detected at least comprise zearalenone and fumonisin B1. The kit and the method have the advantages of high sensitivity, good specificity and the like, have important practical significance on ultra-sensitive detection of multiple fungaltoxins, and have good guiding significance on realization of a field rapid and ultra-sensitive detection technology.

The invention discloses a suspension chip detection method for simultaneously detecting various mycotoxins (aflatoxin B1, vomitoxin, T-2 toxin, and zearalenone). Complete antigens of the mycotoxins and carboxylated microspheres with different codes are coupled through an EDC method, and covalent binding is allowed. With a competition method, a suspension chip technology research is carried out. During detection, the conjugate and monoclonal antibodies of the mycotoxins are oscillated; standard products are added, such that corresponding small molecules of a detection target and the complete antigens compete for the limited monoclonal antibodies; centrifugation is carried out, and supernatant is removed; a biotinylated secondary antibody is added; centrifugation is carried out, and supernatant is removed; SA-PE is added, such that a composition of detection target antigen-monoclonal antibody-secondary antibody-SA-PE is obtained; a median fluorescence value is reduced with the increasing of the concentration of the small molecules, such that a multichannel competition standard curve is drawn. Methanol/water with a certain ratio is used for pre-treating peanut and corn, and multichannel standard curves in corn and peanut are respectively drawn with a negative treatment liquid as a diluting liquid. Through the researches on matrix effect, method accuracy, and method repeatability, various mycotoxins in corn and peanut can be simultaneously detected with the suspension chip method. With the process, a sensitivity reaches a pg-ng level, the result is stable and reliable, sample dose is low, and the method is simple and fast. According to the invention, a novel method is provided for the detection of various mycotoxins.

The invention discloses a kit for synchronously detecting six fungal toxins. The six toxins are aflatoxin, ochratoxin, patulin, trichothecene mycotoxin, fumonisins and zearalenone; and based on a multiple PCR detection method, the kit comprises a first primer pair, a second primer pair, a third primer pair, a fourth primer pair, a fifth primer pair, a sixth primer pair and an internal standard primer pair which can perform PCR reaction in the same reaction tube. The detection system directly targets a toxin synthesis essential gene and has high specificity; an internal reference gene is amplified synchronously, positive, negative and blank control are set simultaneously, and false negative and false positive are effectively avoided; and multiplication of PCR index guarantees the sensitivity of the method.

A SERS detection method for ochratoxin A involves the detection of mycotoxins. The method comprises the steps of preparing an Au nanoparticle sol by reducing chloroauric acid with sodium citrate, preparing TGA-modified gold nanoparticles, and performing quantitative detection in OTA. By taking the gold nanoparticles modified with thioglycolic acid as the SERS substrate, on the one hand, the surface energy of gold nanoparticles is reduced, the stability of gold nanoparticles is improved and the agglomeration of nanoparticles is prevented, and on the other hand, a hydrogen bond is formed by means of the free carboxyl in TGA molecules and carbonyl oxygen in OTA molecules, the OTA is pulled into the local electromagnetic field enhancement region of the gold nanoparticle, that is, the SERS hotspot region, and the Raman detection signal is enhanced. By using the linear relationship between the signal intensity and concentration of the characteristic Raman peak at 1265cm-1 of OTA, a simple,rapid and low-cost quantitative analysis method by directly using SERS characteristic peak of OTA is established, without uncomplicated specificity modification.

The invention relates to an immunomagnetic adsorbent based on a phenylboronic acid directional coupling antibody and a preparation method thereof. According to the immunomagnetic adsorbent, the antibody directional coupling on the surface of a magnetic carrier is realized by utilizing the affinity of a cis-diol structure in an antibody Fc fragment glycoprotein molecule and the specificity of a phenylboronic acid group. Compared with a traditional method, the method has the advantages that the utilization rate of the Fab end of the antibody is increased to the maximum extent, then the affinityand adsorption capacity of the immunomagnetic adsorbent to a target object are improved, the batch-to-batch difference can be effectively controlled, and the method is worthy of further application and popularization. The prepared immunomagnetic adsorbent can be used for detection pretreatment of various food samples, and can be used for enrichment and purification of harmful substances such as mycotoxin, pesticide and veterinary drug residues, heavy metals and pathogenic microorganisms in the food samples according to different coupled antibodies. The purified harmful substances can be quantitatively or qualitatively detected by high performance liquid chromatography, an immunoassay method, an enzyme-linked immunosorbent assay technology and the like.