229 results about "Fungal Toxins" patented technology

A feed nano additive for adsorbing fungal toxin in feed is prepared through proportionally mixing montmorillonite, sodium chloride and water, stirring, water washing, preparing pulp again; adding glucomannan while high-speed stirring, reaction, ageing, water washing, dewatering; drying filter residue and breaking. Its advantages are broad-spectrum adsorption of different toxins, high adsorptive power, no influence of pH value, low residual and low dosage (0.02-0.2%).

The invention relates to an immunochromatography time resolution fluorescence kit for synchronously detecting mixed pollution of five types of fungaltoxinfungi toxins of aflatoxin and the like and application. The immunochromatography time resolution fluorescence kit comprises an immunochromatography time resolution fluorescence test paper strip and sample reaction bottles of monoclonal antibody freeze-drying samples containing europium labels, wherein the fluorescence test paper strip comprises a PVC (polyvinyl chloride) substrate; a water absorbing pad, a detection pad and a sample pad are sequentially adhered onto one surface of the substrate; the adjacent pads are overlapped and connected at the connecting part; the detection pad uses a nitrocellulose membrane as a base pad, and is provided with a transverse quality control line and five detection lines from top to bottom to respectively coat the bovine serum albuminaflatoxin B1-BSA conjugate of each toxin; a fumonisin B1 monoclonal antibody is excreted by a hybrid tumor cell strain Fm7A11 with collection number of CCTCC NO.C201636. The immunochromatography time resolution fluorescence kit is applied to synchronously detect the mixed pollution of toxins of aflatoxin B1, fumonisin B1, ochratoxin A, zearalenone and aspergillus versicolor, and has the advantages that the operation is simple and quick, and the sensitivity is high.

The invention relates to a method for detecting the content of mycotoxins in wheat. The mycotoxins in the wheat are detected by utilizing high performance liquid chromatography-tandem mass spectrometry, and the mycotoxins related to the detection are deoxynivalenol (DON), 3-acetyl (Ac)-DON, 15-Ac-DON, zearalenone (ZEN) and T-2 respectively. The detection method specifically comprises the following steps of: crushing wheat samples by using a crusher, and obtaining coarse extract by using an extracting solution; obtaining a purified toxin extracting solution by using an amino column; preparing mixed toxin standard liquid with different content; detecting each mycotoxin in the toxin extracting solution by adopting the high performance liquid chromatography-tandem mass spectrometry; and obtaining a regression equation of mycotoxin content relative to a peak area according to detection results of the mixed toxin standard liquid, and calculating the content of the DON, the 3-Ac-DON, the 15-Ac-DON, the ZEN and the T-2 in the wheat sample.

The invention provides a fungaltoxin duplex detection method based on Raman beacon molecular coding silver @ gold core-shell nanometer particles, and belongs to the field of material chemical application. The invention has the main content of providing a simple and controllable preparation method of the Raman beacon molecular coding silver @ gold core-shell nanometer particles. A novel multiplex Raman sensing detector is built; the simultaneous fast specificity detection of two kinds or even various kinds of fungaltoxin is realized. The Raman beacon molecules are modified on the surfaces of silver nanometer particles; then, the growth of a layer of gold shell is performed; different Raman beacon molecular coding silver @ gold core-shell nanometer particles are prepared through regulating and controlling the pH of a buffer system and the types of the Raman beacon molecules; the Raman beacon molecule Raman signal intensity change and the generating mechanism under the electromagnetic field coupling effect between silver cores and gold shells are studied; the fungaltoxin aptamer specificity recognition principle and the good magnetic response of magnetic nanometer particles are combined; the multiplex Raman sensing detector is built; the simultaneous fast and specific detection of the aflatoxin and the ochratoxin on the basis of the Raman signals is realized for the first time.

The invention provides a surface-enhanced Raman detection method for mycotoxin based on a silica-coated gold nanotriangle. According to the method, a three-step seed induction method is employed for preparation of a gold nanotriangle, and the gold nanotriangle is labeled with a 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) signal molecule and coated with silica so as to prepare a gold@DTNB@silica surface Raman enhancing agent; a chitosan ferriferrous oxide (CS-Fe3O4) magnetic material is prepared at the same time; an enhanced substrate and the magnetic material are modified with mycotoxin aptamer so as to construct a mycotoxin detection system; when mycotoxin exists, gold@DTNB@silica and CS-Fe3O4 are bonded together via the effect of the specific recognition of the aptamer so as to form a CS-Fe3O4-aptamer-mycotoxin-gold@DTNB@silica detection system; with changes of the concentration of mycotoxin, the Raman signal of the system is not changed after magnetic separation; and thus, quantitative ultra-sensitive detection of mycotoxin in food is realized. The method is applicable to the technical fields of food safety, material chemistry and the like.

The invention relates to a preparation method and use of a CdS-Fe3O4-based electrochemiluminescence sensor and belongs to the field of electrochemiluminescence sensors. A CdS quantum dot is used as an electrochemiluminescence signal source, an antibody is effectively immobilized on the surface of a CdS-Fe3O4 nanometer compound by excellent biocompatibility and a large specific surface area of mesoporous ferroferric oxide, and the CdS quantum dot is immobilized on the surface of the mesoporous ferroferric oxide by a covalent bond so that an antibody capture substrate is obtained. The CdS-Fe3O4-based electrochemiluminescence sensor can realize fungaltoxin and hormone detection according to different electrochemiluminescence signal intensity values of different concentrations of objects to be detected.

The invention discloses an acinetobacter strain and application thereof to degradation of zearalenone. The acinetobacter strain disclosed by the invention is Acinetobacter sp. SM04, and is preserved in the China General Microbiological Culture Collection Center on December 5th, 2011, and the preservation number is CGMCC No. 5524. The Acinetobacter sp. SM04 disclosed by the invention has stronger degradation capability towards mycotoxins ZEN, can degrade more than 98% of zearalenone into low-estrogenic-activity product within 36 hours, cannot produce high-estrogenic-activity analogues such as zearalenone and zeranol, and has real detoxification capability. The acinetobacter strain can be applied to the treatment of corn grains, corn alcohol residues or other mycotoxin contaminated feed, so that safe food and animal feed are obtained, and further, a zearalenone degradation strain which has low cost and high efficiency and avoids secondary pollution is provided for the environment-friendly processing and treatment of the grains and feed.

The invention discloses a detection method for 16 kinds of fungaltoxin. The detection method comprises the following steps of extracting: weighing an accurate sample, putting into a centrifugal pipe,adding an extracting solution of an acetonitrile-water-acetic acid mixed solution, extracting in a vortex or oscillating mode, then centrifuging, sucking liquid supernatant into the centrifugal pipe,adding water into the centrifugal pipe, evenly mixing in a vortex mode, centrifuging, making the liquid supernatant pass through a polytetrafluoroethylene filter membrane and collecting to obtain filtrate A; adding stable isotopes: respectively and accurately sucking the filtrate A and a mixed standard solution of the 16 kinds of fungaltoxin into different internal insertion pipes, adding a mixedwork solution of 14 kinds of fungaltoxin stable isotopes and evenly mixing in a vortex mode. According to the detection method disclosed by the invention, a liquid chromatogram-tandem mass spectrometer is utilized for detection, instrument flexibility is remarkably improved, and the detection can be performed through a direct sample diluting and feeding mode; thus, target loss in a purification process is effectively avoided, dependence on a purifying material is reduced, detection cost is reduced, the sample pretreating efficiency is improved, and quickly detecting multiple components at thesame time is achieved.

The invention relates to the biotechnology field, in particular to a fumonisin degrading enzyme, a coding gene, a recombinant vector, a cell, an additive and application thereof, and more specificallyrelates to the fumonisin degrading enzyme with a sequence shown as SEQ ID NO:2 or its mutant, the coding gene of the enzyme, the vector and cell containing the coding gene, the additive containing the enzyme and / or cell and / or the fermentation product thereof, application of the enzyme, the coding gene, the vector, the cell or the additive in degradation of fumonisins and / or other fungal toxins,as well as a method for degradation of fumonisin / or other fungal toxins. The enzyme provided by the invention has the advantages of: environmental friendliness, efficient and short time degradation offumonisins, and no production of harmful by-products. The enzyme can tolerate catalysis at high temperature up to 70DEG C, has low requirement for pH value and good stability, and also can degrade vomitoxin and T2 toxin to a certain degree.

The invention relates to a preparation method and application of a mycotoxin and hormone electrogenerated chemiluminescence sensor constructed based on NPCo / Co3O4-Au / RuSi@Ru(bpy)3<2+>, and belongs to the field of electrogenerated chemiluminescence sensors. RuSi@Ru(bpy)3<2+> is taken as an electrogenerated chemiluminescence signal source, an antibody is effectively supported to the surface of a nanometer composite NPCo / Co3O4-Au / RuSi@Ru(bpy)3<2+> by virtue of high biocompatibility and large specific surface area of a nanometer porous material NPCo / Co3O4-Au, and RuSi@Ru(bpy)3<2+> is supported to the surface of NPCo / Co3O4-Au to prepare an antibody capturing substrate through a covalent bond. Mycotoxins are detected according to different intensities of electrogenerated chemiluminescence signals of objects to be tested at different concentrations.

The invention provides a method for determining 16 mycotoxins in tea leaves by ultra-high performance liquid chromatography-tandem mass spectrometry, and belongs to the technical field of analytical chemistry. The method comprises: extracting a sample by formic acid / acetonitrile (10: 90); adding a QuEChERS salt packet, shaking and centrifuging; treating an extracting solution through an OASIS PRIME HLB small column and a Dspe purification pipe, separating by using a Waters HSS T3 chromatographic column, adopting a multi-reaction ion monitoring (MRM) mode of simultaneously scanning positive ions and negative ions, matching a tea substrate with a standard solution, and quantifying by using an isotope internal standard method. The method is stable, accurate, sensitive and rapid, and can meetthe requirements of analyzing various toxin residues in various tea leaves.

The invention discloses expression equipment for expressing exogenous protein by secretion in trichoderma reesei cells. The expression equipment comprises the following elements from 5' to 3': (1) an exo-glucan cellobiose hydrolase II promoter of trichoderma reesei; (2) a secretively-expressed signal peptide; (3) a polyclonal locus sequence; and (4) an exo-glucan cellobiose hydrolase II terminator of the trichoderma reesei. Exogenous genes are inserted into the expression equipment, agrobacterium tumefaciens is converted by a T-deoxyribonucleic acid (DNA) binary vector and is jointed with thetrichoderma reesei to obtain trichoderma reesei genetic engineering bacteria which can express heterologous genes from animals, plants, fungi and the like efficiently by secretion, and a large amountof exogenous protein is obtained from the trichoderma reesei genetic engineering bacteria. The trichoderma reesei has an extensive culture condition, and is suitable for solid culture and liquid submerged fermentation; and mycotoxin and antibiotics cannot be generated under the zymogenic condition, and the generated extracellular protein is easy to separate and purify and low in cost.

The invention relates to the field of biotechnology, in particular to an ochratoxin degrading enzyme, a coding gene, a recombinant vector, a cell, an additive and application thereof. The invention more specifically relates to the ochratoxin degrading enzyme with a sequence shown as SEQ ID NO:2 or a mutant thereof, a coding gene of the enzyme, a vector and a cell containing the coding gene, and anadditive containing the enzyme and / or cell and / or a fermentation product thereof, application of the enzyme, the coding gene, the vector, the cell or additive in degradation of ochratoxin and / or other fungal toxins, as well as a method for degradation of ochratoxin / or other fungal toxins. The ochratoxin degrading enzyme provided by the invention is environment-friendly, can efficiently degrade ochratoxin in a short time, and does not generate any harmful by-product. The enzyme can tolerate high temperature catalysis up to 80DEG C, also has low pH value requirement, has good stability, and achieve degradation of fumonisins and T2 toxin to a certain degree.

The invention relates to a matrix solid-phase dispersing agent and application thereof and in particular relates to a solid-phase dispersing agent taking a graphene oxide mixed material as a matrix and an application of the solid-phase dispersing agent, belonging to the technical field of chemistry. A mixed material of graphene oxide and Cleanert Florisil is utilized as a matrix solid-phase dispersing agent, and 18 fungal toxins in a wheat meal sample are measured through a positive ion mode MRM detection mode of high performance liquid chromatography-mass spectrometry / mass spectrometry (HPLC-MS / MS). The matrix solid-phase dispersing agent has the advantages that the recovery rate of a fungal toxin detection method is improved, and the universality of the method is enhanced, so that the detection requirement can be met through a mass spectrum without a positive and negative ion switching mode, and the sensitivity and detection limit can meet the requirements of various standards.

The invention relates to a mushroom polysaccharide composite, which comprises mushroom culture solution and additive. The composite can improve nutrient value, prolong storage life or adsorb and remove harmful substance, such as mycotoxin, heavy metal, pesticide and caffeine.

The invention relates to the technical field of detection of fungal toxins and particularly relates to a method for simultaneously detecting various fungal toxins. The method comprises the following steps: adding samples into an extracting agent for oscillating centrifugation, so as to obtain residues and supernate of the samples; adding methanol and pure water into the residues and taking supernate of the residues; mixing the supernate of the samples and the supernate of the residues to obtain supernate; mixing the supernate and diluent and filtering, so as to obtain extracting solution; enabling the extracting solution to slowly go through a complex immunoaffinity column, draining the extracting solution, performing gradient elution at least twice and collecting eluent; drying the eluent with by blowing nitrogen, so as to obtain to-be-detected solution after constant volume; simultaneously detecting the content of fungal toxins in the to-be-detected solution with LC-MS (liquid chromatography-mass spectrometry), respectively drawing a standard curve of each fungal toxin and finally calculating the content of the fungal toxins in the samples. According to the method, the defect that only one single fungal toxin can be detected with the conventional fungal toxin detection method is overcome, the detection cost is low, the detection efficiency is improved, and the loss and the pollution of a reagent are reduced.

The invention provides a bacterial isolate defined by accession number 040408-1 filed with the International Depository Authority of Canada. The bacteria are capable of detoxifying trichothecene mycotoxins. Also provided are compositions comprising the bacteria and methods of preventing or treating food or foodstuffs that are contaminated or susceptible to contamination with trichothecene mycotoxins. Kits are also provided.

The invention discloses a method for simultaneously detecting seven fungal toxins in original auxiliary materials of baijiu. According to the method, an improved QuEChERS method is adopted for extraction, further purification is not needed, high performance liquid chromatography is utilized for achieving separation of the fungal toxins, combined quadrupole-electrostatic field orbitrap mass spectrometry (Q-Orbitrab) is achieved, efficient parent ion selectivity and high-resolution accurate molecular weight (The mass number can be accurate to five digits after the decimal point.) are combined, accurate qualitativeness and quantification of the fungal toxins in baijiu and the original auxiliary materials can be achieved, and quantification is conducted through a matrix external standard method. The fungal toxins can be completely and effectively separated within 9 min, the linear relation of the fungal toxins within separate linear response ranges is good, the correlation coefficient R<2> is larger than 0.999, the lowest limit of quantification is lower than 1.0 microgram / L, the lowest detection limit is lower than 0.1 microgram / L, the recovery rate ranges from 70% to 120%, and the relative standard deviation (RSD) ranges from 0.5% to 4%. Compared with the national standard method, the method has the advantages of being rapid, simple, convenient, economical, efficient, safe and the like and can be suitable for separation and quantitative determination of various fungal toxins in baijiu and original auxiliary material samples.

The invention discloses a method for detecting mycotoxin in fermented tea. The method comprises dissolving a fermented tea sample in a polar solution, carrying out salting-out, carrying out centrifugation, carrying out filtration, putting the filtrate in 1290 / 6460 ultra-high performance liquid chromatography-triple quadrupole mass spectrometer, carrying out analysis through a mobile phase (0.1% formic acid-5 mmol / L ammonium acetate-water)-methanol, wherein the mobile phase A is 0.1% formic acid-5 mmol / L ammonium acetate-water and the mobile phase B is methanol, and carrying out separation through a gradient elution method comprising improving a concentration of the mobile phase B step by step so that the mycotoxin in the fermented tea sample reaches to the base line in short time and then is separated. The method realizes effective extraction and determination of various mycotoxins in fermented tea, can detect a variety of mycotoxins at the same time and has the characteristics of high detection efficiency, low environmental pollution, simple operation and promotion and use easiness.

The invention discloses a zearalenone (ZEN) toxin degrading enzyme for acinetobacter and a coding gene and applications of the ZEN toxin degrading enzyme. The amino acid sequence of the ZEN toxin degrading enzyme for the acinetobacter is shown by SEQ ID NO.6. The sequence of the coding gene of the ZEN toxin degrading enzyme for the acinetobacter is shown by SEQ ID NO.5. The ZEN toxin degrading enzyme for the acinetobacter performs stronger degrading capacity on mycotoxin ZEN, is capable of degrading at least 90 percent of ZEN into a low-estrogen active product in 12h without generating high-estrogen active analogues, such as ZEN, zeranol and the like, and has a real detoxification effect. According to the invention, the coding sequence of the ZEN toxin degrading enzyme for acinetobacter sp.SM04 is determined, i.e. a Prx gene, and thereby, a foundation is laid for researching the active site of the enzyme and changing the Prx enzyme activity through site-specific mutagenesis in the future.

The invention relates to immunochromatography technologies, and aims at providing a bigeminy qualitative fungaltoxin colloidal gold immunochromatography test strip and a preparation method. The test strip comprises a black strip-like polyvinyl chloride backboard taken as a base material, wherein the surface of the test strip is successively provided with a sample pad, a colloidal gold antibody marker fixing pad, a nitrocellulose membrane and an adsorption pad along the length direction of the backboard, and the adjacent pad or the membrane are in overlap joint with each other; and the nitrocellulose membrane is provided with two detection lines and one quality control line which have the same width with that of the backboard and are separated from one another, wherein the quality control line is positioned between the two detection lines. According to the test strip provided by the invention, the colloidal gold, the cost of which is relatively low, is adopted as an antibody marker, a competition method principle is adopted to quickly detect fungaltoxin, the nitrocellulose membrane wraps the two detection lines simultaneously, the color depth can be compared by naked eyes, and qualitative detection can be carried out quickly on two kinds of fungaltoxin simultaneously. The chromatography test strip prepared has excellent sensitivity and stability, and is easy, convenient and fast to operate and low in cost, thereby being particularly suitable for rapid diagnosis on site.

The invention belongs to the field of micromolecular detection, and relates to a method and a kit for fluorescence detection of micromolecular mycotoxin based on a metal organic framework and upconversion nanoparticles. The method comprises the following steps: S1, obtaining an up-conversion nanoparticle probe modified with a micromolecular mycotoxin aptamer; S2, carrying out synthesis and activation of MIL-101 (Cr); S3, combining the micromolecular mycotoxin with the aptamer; and S4, carrying out signal detection: detecting a fluorescence signal of a product obtained by the reaction in the step S3 by adopting a fluorospectro photometer. The upconversion nanoparticles doped with rare earth are adopted, the fluorescence intensity is stable, the background value is low, and compared with other methods, the fluorescence detection kit does not need complex operation steps, is high in applicability, high in detection result sensitivity and good in specificity, and can be applied to rapid detection of on-site samples.

The invention relates to an irradiation degradation processing method of fungaltoxin. The method comprises the following steps: (1) a fungaltoxin standard substance is prepared into a standard solution; the standard solution is subjected to the irradiation processing with the irradiation range of 0-200 kGy; the content of the fungaltoxin is measured through a liquid chromatogram-tandem mass spectrometry; (2) a toxic sample is subjected to the preprocessing after the irradiation with the irradiation range of 0-9 kGy; the degradation effect of the fungaltoxin is measured through the liquid chromatogram-tandem mass spectrometry; (3) the fungaltoxin standard solution with the highest concentration is selected as a sample; after the selected sample is subjected to the irradiation with the irradiation range of 0-200 kGy, a degradation product is subjected to detection analysis through the liquid chromatogram-tandem mass spectrometry. Through the adoption of the irradiation degradation processing method of the fungaltoxin, the research and environmental protection application of the fungaltoxin degradation product lacking the irradiation degradation are solved, good degradation effect is achieved, and the environmental-friendly detoxification processing for mildewed agricultural product wastes is achieved.

The present invention relates to an immunochromatography time resolution fluorescence kit for synchronization detection of mixed pollution of fumonisin B1 and other four fungaltoxin and application thereof. The immunochromatography time resolution fluorescence kit comprises an immunochromatography time resolution fluorescence test paper strip and a sample reaction bottle containing europium-labeled freeze-dried products of all monoclonal antibodies; the immunochromatography time resolution fluorescence test paper strip includes a PVC substrate, a water absorbent pad, a testing pad and a sample pad which are respectively pasted on one side of the PVC substrate from top to bottom, the testing pad is provided with a nitrocellulose membrane as a base pad, the nitrocellulose membrane is provided with a horizontal quality control line and four detection lines from top to bottom, the four detection lines are respectively coated with bovine serum albumin conjugates of all the fungaltoxin, and a fumonisin B1 monoclonal antibody is secreted by hybridoma cell line Fm7A11 with preservation number of CCTCCNO.C201636. The immunochromatography time resolution fluorescence kit can be used for synchronization detection of content of aflatoxin B1, ochratoxin A, zearalenone and the fumonisin B1, and has the characteristics of simple operation, rapidness and high sensitivity.

The invention provides an intelligent nitrogen-enriched ozone atmosphere-controlling quality-guaranteeing freshness-preserving method and an intelligent nitrogen-enriched ozone atmosphere-controlling quality-guaranteeing freshness-preserving device for foodstuff storage and transportation process. The device consists of a nitrogen and oxygen separation system, an ozone generation system, a temperature and humidity and gas concentration test control system, a direct circulation air supply system, a 360-degree uniform flow atmosphere control air supply system, and an intelligent control system. According to the method and the device, stored foodstuff is subjected to circulation atmosphere control, quality adjustment and freshness preservation or quality preservation drying treatment by utilizing the characteristics of nitrogen and ozone and taking air as a carrier, gas composition and temperature and humidity in a barn are changed to damage the growing environment of pests and molds, and the physiological respiration of the foodstuff is adjusted, so that mold resistance and sterilization, moth resistance and insect disinfestation, quality adjustment and freshness preservation, quality preservation drying and safe detoxication can be realized, and aims of economy and practicability and environment-friendly foodstuff storage are fulfilled. Compared with the conventional foodstuff preservation technology, the method and the device not only have the functions of mold and moth resistance and quality keeping, but also have the functions of quality preservation drying and quality adjustment and freshness preservation. By the method and the device, the functions of keeping quality and preserving freshness of the stored foodstuff with over high water content can be realized, the quality of the stored foodstuff can be improved and water content decrement loss is reduced. The device is environment-friendly, and mycotoxins and organic phosphorus pesticide residues in the foodstuff can be degraded, and the device and the method do not cause secondary pollution to the foodstuff and storage and transportation environment.

The invention relates to a method for detecting 9 kinds of mycotoxins in a cassia seed medicinal material. The method comprises the following steps: a step 1: preparation of a standard substance stocksolution; a step 2: preparation of a mixed standard substance stock solution; a step 3: preparation of a standard working solution; a step 4: preparation of a tested object solution; a step 5: preparation of a substrate matching standard solution; a step 6: determination.

The invention discloses a hybrid material graphene / C3N4 for photocatalytically degrading fungaltoxin. The hybrid material graphene / C3N4 with a layer-layer-layer assembly structure is prepared from graphene oxide and a nanometer photocatalyst g-C3N4 according to the mass ratio being (0.1-10):100 through a hydro-thermal synthesis method. In addition, the influence of the hybrid material graphene / C3N4 on photocatalytic degradation of the fungaltoxin is inspected through the conditions of the graphene modifying amount, photodegradation time and the like. The high-activity hybrid material graphene / C3N4 with visible-light responses is prepared through the hydrothermal method, the process is simple, the hybrid material graphene / C3N4 is suitable for industrial volume production, the photocatalytic degradation technology is applied to the field of fungaltoxin degrading, and high application prospects and practical value are achieved.

The invention relates to the technical field of analysis and detection, and in particular, relates to a detection kit for multiple fungaltoxins, and a preparation method, a detection method and application of the detection kit. The kit comprises up-conversion nanoparticles respectively coupled with antigens of fungaltoxins to be detected, magnetic nanoparticles respectively coupled with antibodies of the fungaltoxins to be detected, and a standard substance solution of the fungaltoxins, and the fungaltoxins to be detected at least comprise zearalenone and fumonisin B1. The kit and the method have the advantages of high sensitivity, good specificity and the like, have important practical significance on ultra-sensitive detection of multiple fungaltoxins, and have good guiding significance on realization of a field rapid and ultra-sensitive detection technology.

The invention discloses a suspension chip detection method for simultaneously detecting various mycotoxins (aflatoxin B1, vomitoxin, T-2 toxin, and zearalenone). Complete antigens of the mycotoxins and carboxylated microspheres with different codes are coupled through an EDC method, and covalent binding is allowed. With a competition method, a suspension chip technology research is carried out. During detection, the conjugate and monoclonal antibodies of the mycotoxins are oscillated; standard products are added, such that corresponding small molecules of a detection target and the complete antigens compete for the limited monoclonal antibodies; centrifugation is carried out, and supernatant is removed; a biotinylated secondary antibody is added; centrifugation is carried out, and supernatant is removed; SA-PE is added, such that a composition of detection target antigen-monoclonal antibody-secondary antibody-SA-PE is obtained; a median fluorescence value is reduced with the increasing of the concentration of the small molecules, such that a multichannel competition standard curve is drawn. Methanol / water with a certain ratio is used for pre-treating peanut and corn, and multichannel standard curves in corn and peanut are respectively drawn with a negative treatment liquid as a diluting liquid. Through the researches on matrix effect, method accuracy, and method repeatability, various mycotoxins in corn and peanut can be simultaneously detected with the suspension chip method. With the process, a sensitivity reaches a pg-ng level, the result is stable and reliable, sample dose is low, and the method is simple and fast. According to the invention, a novel method is provided for the detection of various mycotoxins.

The invention discloses a detection method of fungaltoxin DON (deoxynivalenol) and a detection kit. The method comprises steps as follows: DONApt and a single-stranded signal probe ssDNA are hybridized, and a DONApt-ssDNA (single-stranded deoxyribonucleic acid) hybrid chain is formed; a to-be-detected sample is added to the DONApt-ssDNA hybrid chain, and when the to-be-detected sample has DON, DONApt-ssDNA reacts with DON to generate DONApt-DON and release ssDNA; remaining DONApt-ssDNA hybrid chain in the system forms double-stranded DNA through DNA amplification; double-stranded DNA is hydrolyzed into mononucleotide under catalysis of exonuclease and is removed, and ssDNA in the system is reserved; silver ions and a reducing agent are added to the system, and silver ions are reduced to generate near infrared fluorescence silver nano-clusters under induction of ssDNA; finally, the content of DON in the to-be-detected sample is calculated according to the relation between the near infrared fluorescence intensity and the quantity of DON.