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Method and kit for fluorescence detection of micromolecular mycotoxin based on metal organic framework and upconversion nanoparticles

A metal-organic framework and nanoparticle technology, which can be used in fluorescence/phosphorescence, material analysis by optical means, measurement devices, etc., can solve the problems of high detection cost, difficult to popularize and use on a large scale, cumbersome operation steps, etc., to achieve applicability The effect of strong, stable fluorescence intensity and low background value

Active Publication Date: 2021-03-05
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods either have cumbersome operation steps or high detection costs, making it difficult to promote them on a large scale.

Method used

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  • Method and kit for fluorescence detection of micromolecular mycotoxin based on metal organic framework and upconversion nanoparticles
  • Method and kit for fluorescence detection of micromolecular mycotoxin based on metal organic framework and upconversion nanoparticles
  • Method and kit for fluorescence detection of micromolecular mycotoxin based on metal organic framework and upconversion nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] This example is used to illustrate the rapid detection kit and detection method of T-2 toxin of the present invention. The specific implementation steps are as follows:

[0047] 1) Preparation and modification of upconversion nanoparticles modified with small molecule mycotoxin aptamers

[0048] (a) 0.78mmol YCl 3 ·6H 2 O, 0.2mmol YbCl 3 ·6H 2 O and 0.02 mmol TmCl 3 ·6H 2 O was dissolved in 4 mL of aqueous solution and added to a 100 mL three-necked flask containing 9 mL of OA and 15 mL of 1-ODE.

[0049] (b) The solution was stirred at room temperature for 10 minutes and heated at 150° C. for 1.5 hours to remove water and form the lanthanide oleate complex. At this time, the solution was transparent and light yellow, and then cooled to 50°C.

[0050] (c) Dissolve in 10mL CH 3 2.5 mmol NaOH and 4 mmol NH in OH 4 F was added to the above mixture and stirred at 50°C for 30 minutes. Set the system at N 2 Heated to 100°C in atmosphere for 1 hour to remove CH 3 ...

Embodiment 2

[0060] This example is used to illustrate a method for detecting T-2 toxin in corn flour with a fluorescent aptasensor based on metal-organic frameworks and up-conversion nanoparticles of the present invention. The up-conversion nanoparticles prepared in Example 1 were used to detect Needles and Metal Organic Framework MIL-101(Cr).

[0061] Specifically include the following steps:

[0062] 1) Add T-2 toxin to water at three different concentrations: 1 ng / mL, 5 ng / mL and 20 ng / mL. 1 g of corn flour was mixed with 10 mL of extraction solvent (methanol:water=6:4 (v / v)) containing various concentrations of T-2 toxin.

[0063] 2) The sample was vortexed for 5 minutes, then centrifuged at 13000 rpm for 10 minutes, and the supernatant was collected.

[0064] 3) In the buffer solution containing the upconverting nanoparticle probe (100 μL, 0.5 mg / mL), add 50 μL of each of the supernatants extracted from three types of corn, and incubate at 37° C. for 0.5 h.

[0065] 4) Add an equa...

Embodiment 3

[0069] This example is used to illustrate a method for detecting T-2 toxin in beer with a fluorescent aptasensor based on metal-organic frameworks and upconversion nanoparticles, using the upconversion nanoparticle probe prepared in Example 1 and Metal Organic Framework MIL-101(Cr).

[0070] 1) The beer was refrigerated at 4°C for 30 minutes or degassed by ultrasonic waves before use, and different concentrations of T-2 toxin were added to the beer, and then 10 μL of the mixture was added to 990 μL of 10 mM Tris-HCl to finalize the T-2 toxin. Concentrations were 1ng / mL, 5ng / mL and 20ng / mL. Three concentrations of samples to be tested were obtained.

[0071] 2) Add 50 μL each of the three beer sample solutions to the buffer containing the upconverting particle probe (100 μL, 0.5 mg / mL), and incubate at 37° C. for 0.5 h.

[0072] 3) Add an equal volume of MOFs suspension into a centrifuge tube and mix well, then shake slowly and react at 37°C for 20min.

[0073] 4) Then use a...

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Abstract

The invention belongs to the field of micromolecular detection, and relates to a method and a kit for fluorescence detection of micromolecular mycotoxin based on a metal organic framework and upconversion nanoparticles. The method comprises the following steps: S1, obtaining an up-conversion nanoparticle probe modified with a micromolecular mycotoxin aptamer; S2, carrying out synthesis and activation of MIL-101 (Cr); S3, combining the micromolecular mycotoxin with the aptamer; and S4, carrying out signal detection: detecting a fluorescence signal of a product obtained by the reaction in the step S3 by adopting a fluorospectro photometer. The upconversion nanoparticles doped with rare earth are adopted, the fluorescence intensity is stable, the background value is low, and compared with other methods, the fluorescence detection kit does not need complex operation steps, is high in applicability, high in detection result sensitivity and good in specificity, and can be applied to rapid detection of on-site samples.

Description

technical field [0001] The invention belongs to the field of small molecule detection, and in particular relates to a method for the fluorescence detection of small molecule mycotoxins based on metal-organic frameworks and up-conversion nanoparticles, and a method for fluorescence detection of small molecules based on metal-organic frameworks and up-conversion nanoparticles Mycotoxin kit. Background technique [0002] T-2 toxin belongs to class A trichothecenes secreted by Fusarium in nature, and is the most toxic of such toxins. It is widely found in corn, barley, wheat, oats and other grains . T-2 toxin enters the body of animals and humans along with contaminated food, causing great harm to the health and growth of the body. It can cause a variety of toxic effects in the body, including acute toxicity and chronic toxicity. Symptoms include vomiting, anorexia, and weight loss. In addition, T-2 toxin can also cause damage and apoptosis of liver, brain tissue, nervous sys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6428G01N2021/6417
Inventor 高志贤王瑜赵旭东彭媛李双白家磊韩殿鹏任舒悦秦康宁保安
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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