41 results about "Upconversion nanoparticle" patented technology

The invention discloses a photo-thermal-electric coupling titanium-based implant as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The photo-thermal-electric coupling titanium-based implant provided by the invention comprises a porous titanium substrate and a hydrogel layer, the surface of the porous titanium substrate is of a TiO2 nanotube array structure; up-conversion nanoparticles and g-C3N4 nanoparticles are arranged between the porous titanium substrate and the hydrogel layer, and the up-conversion nanoparticles are NaYF4 up-conversion nanoparticles co-doped with Yb < 3 + > and Er < 3 + >; carbon nanotubes and medicinal active ingredients are loaded in the hydrogel layer. According to the titanium-based implant, collaborative coupling in light-heat-electricity multi-energy forms is achieved, the titanium-based implant is endowed with the integrated function of light response, heat conversion, electric signal conduction and local treatment, and an integrated solution with structural supporting, biological activity regulation and treatment effects is provided for bone tissue defect repair.

The invention relates to the technical field of nano materials and biomedical engineering, and discloses a dye-sensitized rare earth up-conversion luminescent nano material as well as a preparation method and application thereof. The dye-sensitized rare earth up-conversion luminescent nano-material comprises a rare earth doped up-conversion nano-particle core, an unsaturated fatty acid salt self-assembled shell layer and a near-infrared dye modified on the surface of the rare earth doped up-conversion nano-particle. The unsaturated fatty acid salt coating layer endows the material with excellent water dispersibility through a hydrophilic group; a hydrophobic chain segment and an unsaturated double bond can effectively block water and oxygen permeation and quench singlet oxygen, so that the photobleaching half-life period of the material is increased by 86.7 times or more compared with that of a conventional coating material, and the light stability is excellent; the material successfully realizes efficient up-conversion luminescence under excitation of a low-power near-infrared light emitting diode (NIR LED, the power density of which can be as low as 100mW / cm < 2 >), and thoroughly gets rid of dependence on a high-cost and high-risk laser.

The invention relates to the technical field of drug loading systems, and discloses a nanoparticle applied to photodynamic therapy and a preparation method thereof.The nanoparticle is of a core-shell-crown composite structure and sequentially comprises an inner core layer, an outer core layer, an inner shell layer and an outer shell layer from inside to outside, the inner core layer is composed of NaYF4 up-conversion nanoparticles, and the outer core layer is composed of NaYF4 up-conversion nanoparticles; the middle layer is a mesoporous Zr-MOF layer; the shell layer is a pH responsive polymer layer which is formed by connecting a polyethylene glycol-polycaprolactone block copolymer with folic acid through a hydrazone bond; the targeting canopy layer comprises double targeting ligands, and the double targeting ligands are respectively a bicyclo [6.1. 0] nonyne functionalized iRGD peptide and an azide functionalized anti-EGFR (epidermal growth factor receptor) nano antibody 7D12. The nanoparticles have the advantages of high stability, strong targeting property, high photosensitizer loading and the like, and can efficiently generate active oxygen.

The application discloses a multifunctional nanodiagnosis and treatment probe and a preparation method and application thereof, and belongs to the technical field of nanobiomedicine and tumor molecular diagnosis and treatment. The multifunctional nanodiagnosis and treatment probe is prepared by the following steps: coating mesoporous silica on the surface of a core-shell structure upconversion nanoparticle, then adsorbing platinum particles and methylene blue into the mesoporous silica, then modifying polydopamine to the surface of the mesoporous silica coated core-shell structure upconversion nanoparticle on which the platinum particles and the methylene blue are adsorbed, and finally linking nucleic acid PH1 chains and EH2 dendrites to the surface of the upconversion nanoparticle and dispersing and preparing by using a PBS buffer. The multifunctional nanodiagnosis and treatment probe prepared by the application can not only realize specific recognition, quantitative detection and in-situ imaging of a target microRNA, but also can form a synergistic tumor treatment mode of "photodynamic therapy-optical thermal therapy-oxygen enhancement", and has a wide application prospect in early tumor screening, curative effect evaluation and individualized treatment.

The application discloses a kind of near-infrared benzothiadiazole dye sensitized rare earth upconversion luminescence nano-probes and preparation method thereof;Probe is composed of near-infrared benzothiadiazole dye and upconversion nanoparticle, benzothiadiazole dye is compounded with ligand-free lanthanide ion doped upconversion nanoparticle, and upconversion luminescence is enhanced.This application preparation method is simple, and it does not involve complex core-shell structure and energy migration process, benzothiadiazole dye is directly compounded with ligand-free rare earth nanoparticle, and upconversion luminescence nano-probe is formed, under the irradiation of 808nm laser, the emission spectrum of benzothiadiazole dye and the absorption spectrum of sensitizer ion in upconversion nanoparticle overlap, which can effectively enhance the upconversion luminescence intensity of nano-probe, and keep good light stability, keep more than 90% of initial luminescence intensity under the irradiation of 120 minutes excitation light.

The invention provides an anti-tumor targeting nano material and a preparation method thereof, and belongs to the field of novel targeting nano drugs. The method comprises the following steps: stirring and mixing up-conversion nanoparticles, calcium peroxide nanoparticles, manganese chloride and a chemotherapeutic drug in a water phase to obtain a drug-loading core; cystamine and 3-aminophenylboronic acid are dissolved in water, EDC and NHS are added, a first amidation reaction is carried out, and a cross-linking agent is obtained; dispersing the drug-loading core in a polyacrylic acid solution, adding a cross-linking agent, and then carrying out a cross-linking reaction to obtain an intermediate product; activating carboxyl on the surface of the intermediate product, and then carrying out second amidation reaction with cRGD-PEG-NH2, so as to obtain the anti-tumor targeting nano material. According to the application, a multifunctional nano platform integrating targeted enrichment, double-response precise drug release, chemotherapy-chemical kinetics-immune collaborative treatment and up-conversion imaging tracing is constructed, and specific efficient killing of tumors and inhibition of metastasis and relapse of the tumors are realized.

The invention discloses a three-layer rare earth fluoride, an up-conversion nanoparticle, a tumor in-situ vaccine and application. The three-layer rare earth fluoride has a core-shell structure and comprises a core nanocrystal, a first shell layer and a second shell layer from inside to outside, wherein the crystal phase of the core nanocrystal is alpha-NaYbF4, and the core nanocrystal comprises a doping element; the doping element comprises Tm; the composition of the first shell layer comprises NaYbF4; and the composition of the second shell layer comprises NaYF4. The three-layer rare earth fluoride can realize conversion between near-infrared light and ultraviolet light. Furthermore, the up-conversion nanoparticles containing the three layers of rare earth fluorides and the photo-crosslinking agent can be used for efficiently capturing and enriching tumor antigens in situ under the near-infrared light activation condition, and excellent tumor antigen presentation efficiency is ensured; in addition, the tumor in-situ vaccine containing the three-layer rare earth fluoride, the photo-crosslinking agent and the active medicine can obviously improve the in-situ treatment effect.

The present invention discloses a white-light-emitting upconversion rare-earth-doped nanomaterial and a preparation method thereof. The core-shell structured LiYF4:xHo@LiYbF4@LiYF4 upconversion nanoparticles (0 < x ≤ 0.02) with a particle size of 28-38 nm are synthesized by the high-temperature co-precipitation method. They have good dispersibility and obvious upconversion luminescence. Under 980 nm laser excitation, they exhibit upconversion fluorescence at 480 nm, 540 nm and 650 nm, making their overall color show white light, and having good application prospects in the fields of biomedicine, solar cells, temperature sensors and photocatalytic degradation, etc.

The application belongs to the technical field of nanometer luminescent materials, and particularly relates to a near-infrared light response upconversion nanocomposite material, a preparation method and application thereof. The nanocomposite material comprises a rare earth upconversion nanoparticle inner core, a porphyrin metal-organic framework shell layer, a near-infrared dye loaded in the porphyrin metal-organic framework pores, and an amphiphilic phospholipid polymer modified on the outer surface of the porphyrin metal-organic framework. The metal ion in the porphyrin metal-organic framework shell layer is a metal ion with a heavy atom effect, and the metal ion is selected from at least one of Pt(II), Pd(II), Cu(II), Mn(II) or Ir(Ⅳ). The nanocomposite material has excellent singlet oxygen production and photodynamic sterilization effect.

The application discloses a kind of lysosome-targeting protein modulators based on upconversion nanomaterial-DNA nanostructure and preparation method thereof.The lysosome-targeting protein modulators are combined by covalent bond from carboxyl functionalized upconversion nanoparticles and amino-modified DNA nanostructure, mainly enter cancer cells through folate receptor, after near infrared light illumination and glutathione response, the target protein combined is transported into lysosome and degraded.The lysosome-targeting protein modulators of the application have the advantages of light affinity, high controllability and storage stability, are easy to be metabolized by organism, and have good application prospect in the field of cell biology and cancer targeted therapy.

The invention relates to the technical field of medical implantable optical and electrical integrated devices, in particular to a composite functional degradable optical fiber and a preparation method and application thereof. The composite functional degradable optical fiber provided by the invention has the characteristics of biodegradability and high flexibility, permanent foreign body reaction can be avoided, and the biocompatibility is greatly improved. According to the composite functional degradable optical fiber provided by the invention, based on the unique physical characteristics of the up-conversion nanoparticles, the inherent synergistic treatment of PBM and PDT under the driving of a single near-infrared light source is realized. According to the composite function degradable optical fiber provided by the invention, a treatment-feedback closed-loop system architecture of minimally invasive monitoring is integrated, and a good hardware basis is provided for realizing self-adaptive treatment. The composite functional degradable optical fiber provided by the invention can be applied to the field of medical implantation instruments, the field of neural engineering or the field of biological materials, and particularly can be applied to brain implantation materials or brain degenerative disease self-adaptive treatment systems.

The invention provides nanoparticles for tumor photodynamic therapy and a preparation method, a pharmaceutical composition and application thereof, and belongs to the technical field of nano biomedicine. The nanoparticle for tumor photodynamic therapy comprises: an up-conversion nanoparticle; the phospholipid layer is used for coating the up-conversion nano-particles; the nucleic acid aptamer and the endoplasmic reticulum targeting peptide are modified on the surface of the phospholipid layer; and a photosensitizer supported on the surface of the nanoparticle. The nucleic acid aptamer and the endoplasmic reticulum targeting peptide are further co-modified on the basis of the surface of the upconversion nano-particle subjected to phospholipidation treatment, so that the nano-particle can actively recognize tumor cells and deliver the tumor cells to the endoplasmic reticulum, and dual targeting on the tumor cells is realized. The loaded photosensitizer can generate reactive oxygen species (ROS) under excitation of near-infrared light, endoplasmic reticulum stress is specifically induced, then a cell apoptosis pathway is activated, and tumor cells are killed.

The invention provides a high-transparency infrared contact lens and a preparation method thereof, and belongs to the technical field of infrared contact lenses. The high-transparency infrared contact lens is prepared from the following raw materials: organic hydrogel and modified up-conversion nanoparticles, the mass ratio of the organic hydrogel to the modified upconversion nanoparticles is 100: (3-8); the organic hydrogel is poly (2-hydroxyethyl methacrylate) hydrogel or silicon-based hydrogel; according to the invention, a specific type of organic hydrogel is combined with the modified up-conversion nanoparticles, so that near-infrared light invisible to naked eyes can be converted into visible light, and near-infrared light detection and sensing functions are realized; the light transmittance and transparency are good, and the visible light transmittance is not influenced.

This invention relates to the field of drug delivery systems, and discloses a nanoparticle for photodynamic therapy and its preparation method. The nanoparticle has a core-shell-corona composite structure, comprising, from the inside out: a core layer composed of NaYF4 upconversion nanoparticles; a middle layer: a mesoporous Zr-MOF layer; a shell layer: a pH-responsive polymer layer formed by linking folic acid to polyethylene glycol-polycaprolactone block copolymer via hydrazone bonds; and a targeting corona layer comprising dual targeting ligands, namely a bicyclic [6.1.0]nonyne-functionalized iRGD peptide and an azide-functionalized anti-EGFR nanobody 7D12. The nanoparticles of this invention possess advantages such as high stability, strong targeting, and high photosensitizer loading, and can efficiently generate reactive oxygen species.

The invention discloses hydrogel for osteoporotic bone defect repair and a preparation method of the hydrogel. The hydrogel comprises basic hydrogel and mineralized photosynthetic microorganisms, the mineralized photosynthetic microorganism comprises a photosynthetic microorganism, a mineralized shell layer arranged on the surface of the photosynthetic microorganism, and rare earth up-conversion nano-particles loaded in cells of the photosynthetic microorganism, and the rare earth up-conversion nano-particles can convert near-infrared light into visible light. According to the present invention, the oxygen production type mineralized microalgae composite hydrogel with characteristics of deep tissue activation, controllable oxygen production and simultaneous realization of four-effect synergy of acid neutralization, continuous oxygen supply, angiogenesis promotion and osteogenesis mineralization can be provided, such that osteoblast activity can be promoted, osteoclast activity can be inhibited, in-vivo osteogenesis can be promoted, and the technical support can be provided for osteoporotic bone defect repair.

The application provides a kind of molecular self-assembly triplet-triplet annihilation (TTA) photonic upconversion hydrogel and its preparation method and application, select polyethylene oxide-polypropylene oxide-polyethylene oxide (PEO-PPO-PEO) F127 three-block polymer to encapsulate upconversion dye pair, form nanomicelles.Utilize three-block polymer hydrophilic section PEO and hydrophobic section PPO in aqueous solution self-assembly to form nanoparticles, hydrophilic end is exposed outside, form dense oxygen barrier shell, hydrophobic end and hydrophobic luminescent material in core, avoid being quenched by oxygen in air.Again, add aqueous solution containing upconversion nanoparticles into acrylamide aqueous solution, directly polymerize, obtain upconversion hydrogel.The strategy is not only simple operation, without complex modification to luminescent material, and does not contain volatile organic solvent, and polyacrylamide hydrogel also has good biocompatibility, so it has great application prospect in biological field.

This invention discloses a method for preparing and applying nanochloroplasts with targeted mitochondrial repair function, belonging to the field of biomedical technology. The preparation method includes the following steps: mixing energy module nanoparticles and catalytic module nanoparticles with a cell membrane solution, and co-encapsulating them in a compartmentalized manner using membrane extrusion technology to form a nanochloroplast core; then, co-modifying the surface of the nanochloroplast core with upconversion nanoparticles through asymmetric modification. The nanochloroplasts of this invention can simulate the partitioned structure of natural chloroplasts, achieving phototaxis, energy supply, and carbon fixation functions, and can target mitochondria for restoring mitochondrial metabolism. This system can reconstruct the mitochondrial oxygen-carbon metabolism network in an ischemic model and is expected to develop into a novel nanomaterial for more effective treatment of ischemic diseases.

A core-shell nanoparticle having a cleaned nitrogen-vacancy nanodiamond (NVND) core surrounded by an upconversion nanoparticle (UCNP) shell, wherein the cleaned nitrogen-vacancy nanodiamond (NVND) core comprises a cleaned nitrogen-vacancy nanodiamond, wherein the UCNP shell comprises an upconversion nanoparticle (UCNP), wherein the UCNP comprises lithium yttrium fluoride (LiYF4) doped with a lanthanide ion combination, wherein the lanthanide ion combination comprises a M1 ion and a M2 ion, wherein M1 is ytterbium (Yb) or neodymium (Nd) and M2 is erbium (Er), thulium (Tm) or holmium (Ho), and wherein the core-shell nanoparticle emits a red-emission upon a near-infrared (NIR) excitation is disclosed. Integration of cleaned NVNDs with UNCPs for enhancing optical manipulation and enabling efficient energy transfer for applications in biological imaging, quantum sensing and laser cooling is disclosed.

The invention discloses a nonlinear photoetching method based on rare earth up-conversion nanoparticles, and belongs to the technical field of micro-nano processing and manufacturing. The core of the method is as follows: preparing a photoresist of Yb / Tm co-doped up-conversion nanoparticles; low-power 976 nm continuous laser is adopted as an excitation light source, and low-energy near-infrared light is converted into 455 nm visible light by utilizing the nonlinear up-conversion effect of nanoparticles, so that a photoinitiator in photoresist is activated, and polymerization reaction of the photoresist is initiated. And high-resolution microstructure processing is realized by controlling the laser power, the scanning speed and the concentration of the photoinitiator. According to the method, the dependence on high-power femtosecond pulse laser is reduced, the system cost is remarkably reduced, and the thermal damage effect in the machining process is thoroughly eliminated. Experiments show that the method can obtain a clear polymer structure with the characteristic size of 3 microns under low-power continuous laser of 20 mW. The invention provides a new scheme with low cost, high precision and no thermal damage for micro-nano processing, and has wide application prospects in the fields of semiconductor, biomedicine and micro-optical device preparation.

The application discloses a kind of supermolecular hybrid hydrogel continuously generating hydroxyl radical, wherein the supermolecular hybrid hydrogel is composed of sodium hyaluronate and a nanocomposite, the sodium hyaluronate is the substance forming the network structure of the supermolecular hybrid hydrogel, the nanocomposite is distributed in the network structure, and the nanocomposite is the complex of beta-cyclodextrin modified upconversion nanoparticle and (ferrocene methyl) trimethyl ammonium iodide;Wherein the mass ratio of beta-cyclodextrin modified upconversion nanoparticle and (ferrocene methyl) trimethyl ammonium iodide complex: sodium hyaluronate: deionized water is 52: (17.5-20): 1000.The supermolecular hybrid hydrogel of the application can catalyze the reverse Fenton reaction under the tumor microenvironment, in situ cyclic generation of hydroxyl radical, and has potential application in the treatment of malignant tumors.

The invention discloses a method for detecting malachite green based on a fluorescence resonance energy transfer principle, a biosensor and a kit, and belongs to the technical field of aquatic product quality safety detection. According to the method, near-infrared light excited lanthanide doped up-conversion nanoparticles (UCNPs) are used as an energy donor, and a nucleic acid aptamer for specifically recognizing malachite green is covalently linked to the surface of the UCNPs to form the nano probe. When the malachite green does not exist in the sample, the aptamer is in a flexible conformation, and the fluorescence of the UCNPs is quenched due to the FRET effect. When malachite green exists in a sample, the aptamer is specifically combined with the malachite green and is subjected to conformational change, so that the spatial distance between the nanoprobe and a quenching agent is increased, the FRET process is blocked, and UCNPs fluorescence is recovered. The qualitative and quantitative analysis of malachite green can be realized by detecting the change of a fluorescence signal. The method has the advantages of high sensitivity, strong specificity, simple operation and rapid response, and is suitable for on-site rapid detection of malachite green residues in aquatic products and aquatic water.

This invention discloses a near-infrared light-responsive thermo-electrically coupled titanium-based implant, its preparation method, and its application, belonging to the field of biomedical materials technology. The near-infrared light-responsive thermo-electrically coupled titanium-based implant provided by this invention comprises a porous titanium substrate and a composite hydrogel coating; the surface of the porous titanium substrate is sequentially disposed from the inside out with TiO2 / SrTiO3 heterojunctions, upconversion nanoparticles, and barium titanate nanoparticles; the upconversion nanoparticles are Yb. 3+ and Er 3+ Co-doped NaYF4 upconversion nanoparticles; a composite hydrogel coating is deposited on the surface of barium titanate nanoparticles, the composite hydrogel coating comprising MXene, pharmaceutically active ingredients, and a hydrogel carrier. This invention achieves synergistic coupling of photothermal and photoelectric-pyroelectric effects under near-infrared light irradiation by constructing a multi-layered synergistic structure on a porous titanium substrate, thereby synergistically realizing multiple functions such as promoting bone growth, antibacterial activity, and antitumor activity.

The invention relates to application of chlorin-loaded rare earth upconversion nanoparticles and an anti-tumor and anti-inflammatory drug, and relates to the field of biological medicines. The chlorin-loaded rare earth up-conversion nanoparticles are used for preparing anti-tumor or / and anti-inflammatory drugs. The chlorin-loaded rare earth up-conversion nanoparticles disclosed by the invention do not generate toxicity to normal cells in a concentration range of 31.25-500mu g / ml, and can effectively inhibit migration of tumor cells and inflammation related mechanisms; the tumor targeting agent has no toxicity to mice within 120 mg / kg in mice, can target tumor sites and reach lungs through blood circulation, and 20 mg / kg of the tumor targeting agent can significantly inhibit tumor volume and tumor pulmonary metastasis.

The invention relates to the technical field of luminescent nano materials, in particular to core-shell structure up-conversion nano particles with efficient red luminescence performance and a preparation method of the core-shell structure up-conversion nano particles. The nanoparticle is of a core-shell structure, a core layer comprises a BaF2 matrix, and Er < 3 + > and Ga < 3 + > are doped in the BaF2 matrix; the shell layer comprises a CaF2 matrix, and Yb < 3 + > and Mn < 2 + > are doped in the CaF2 matrix. According to the invention, the up-conversion luminous efficiency, luminous intensity and purity are improved at the same time, and the core-shell structure up-conversion nano-particle with high purity and high-efficiency single-color red light emission is provided.

The invention discloses an excitation method and a detection system for improving the quantum efficiency of upconversion nanoparticles, and belongs to the technical field of fluorescence detection. The excitation method comprises the steps that pulse laser serves as a laser light source to irradiate the up-conversion nanoparticles, the pulse width range of the pulse laser is 50 microseconds to 800 microseconds, the frequency range of the pulse laser is 1.27 KHz to 20 KHz, and the power of the pulse laser is 4.5 W. By adopting the pulse laser as an excitation light source, the quantum efficiency of the up-conversion nanoparticles is improved to about 0.52 + / -0.025% from about 0.22 + / -0.018% under traditional direct-current light excitation, the optical performance of the up-conversion nanoparticles is effectively improved, and possibility is provided for application of the up-conversion nanoparticles in more fields.

This invention discloses a competitive homogeneous immunoassay method based on upconversion nanoparticles. The method involves conjugating activated upconversion nanoparticles to an antigen; enriching and purifying the upconversion nanoparticle-antigen probe; mixing the donor with specific antibodies and free antigens at different concentration gradients, followed by an immunoassay; receptor labeling; and detecting the fluorescence intensity of the reaction solution. The concentration of the antigen in the sample to be tested is obtained from the curve of the change in free antigen concentration versus fluorescence intensity, or the concentration of the target analyte is obtained at the single-particle level by counting the quantitative changes in the immune complex. This invention effectively solves the problems of high background noise and low signal-to-background ratio in resonance energy transfer (RET). By labeling the formed semi-sandwich structure immune complexes using a receptor deposition method, receptors accumulate around the immune complexes, increasing the number of receptors and shortening the distance between the donor and receptor, significantly improving RET efficiency and signal strength, thereby enhancing the sensitivity of the competitive homogeneous immunoassay.

This invention discloses a highly sensitive LCN2 detection kit and its preparation method for assessing the prognostic risk of patients with stress-induced cardiomyopathy. The kit contains a signal probe composed of rare-earth-doped upconversion nanoparticles and an LCN2-specific DNA aptamer, as well as a complementary oligonucleotide quenching probe with a quenching group. Its core principle is based on fluorescence resonance energy transfer regulated by competitive binding: when the target protein LCN2 is absent, the binding of the two probes leads to luminescence quenching; when LCN2 is present, they competitively bind to the aptamer and dissociate the quenching probe, thereby restoring upconversion luminescence. This homogeneous detection method effectively avoids the interference of autofluorescence in biological samples by utilizing near-infrared excitation. Combined with the high stability and specificity of the aptamer, it achieves a simple, rapid, highly specific, and ultra-sensitive quantitative detection of LCN2 protein in serum, providing a reliable tool for clinical prognostic assessment.

A biosensing platform for in-situ sampling and target detection based on upconversion luminescence, including: an upconversion luminescent paper-based microfluidic device, an upconversion luminescent biosensor, and a portable detection device based on smartphone imaging. The upconversion luminescent paper-based microfluidic device is configured to sample a to-be-detected substance in situ. The upconversion luminescent biosensor is configured to allow a target to specifically recognize the to-be-detected substance. The portable detection device is configured to detect a content of the to-be-detected substance. The upconversion luminescent biosensor is prepared as follows. (S1) An upconversion nanoparticle seed is prepared. (S2) Core-shell upconversion nanoparticles are prepared. (S3) Core-shell-shell upconversion nanoparticles (UCNPs) are prepared. (S4) The UCNPs is subjected to hydrophilic modification. (S5) The hydrophilically-modified UCNPs are modified with DNA. (S6) The DNA-modified particles obtained from step (S5) are modified with a dye. The to-be-detected substance is 17β-estradiol (17β-E2).

This invention discloses a computationally adaptive aberration correction imaging method for deep tissue based on upconversion nanoparticle guide stars. A near-infrared fluorescence imaging system is constructed using rare-earth upconversion nanoparticles as guide stars. Fluorescence distortion images of deep tissue samples are acquired through near-infrared excitation, and imaging aberrations are parameterized based on Zernike polynomials. Aberration coefficients are reconstructed using a deep learning model incorporating physical prior constraints. Digital wavefront compensation is performed based on the reconstructed aberration information to achieve deep tissue aberration correction and obtain high-resolution imaging results. This invention eliminates the need for complex optical hardware such as wavefront sensors or deformable mirrors, and can stably achieve aberration estimation and compensation even in 360 μm deep tissue conditions. Compared with traditional aberration correction methods, the correction effect is improved by more than 30%, enabling rapid image correction and high-precision positioning. It solves the problems of weak guide star signals, low aberration correction efficiency, and high system cost in deep tissue imaging, and is applicable to deep tissue optical imaging in fields such as clinical diagnosis, biological cell observation, and drug development.

The invention belongs to the technical field of inorganic nano materials, and discloses a pH response type core-shell multilayer rare earth nano drug-loading platform for multi-modal imaging therapy, which comprises core-shell upconversion nanoparticles subjected to surface functionalization modification through BSA (Bovine Serum Albumin), according to the core-shell type up-conversion nanoparticle, a fluoride nanoparticle is used as a core, Cu2-xSe is used as a photosensitizer loaded on the fluoride nanoparticle, and ZnFe2O4 is used as a shell wrapping the fluoride nanoparticle and the photosensitizer; wherein the fluoride nano particles are alpha-NaYbF4: 2.5% Tm (Tm). In conclusion, the rare earth nano material with the cubic crystal phase is reasonably combined with the photosensitizer, the photo-thermal preparation and the imaging preparation to obtain a nano anti-tumor treatment platform, so that the effects of photodynamic treatment under guidance of multi-mode imaging, Fenton reaction induced ferroptosis of tumor cells and photo-thermal treatment combined anti-tumor treatment can be realized; and meanwhile, drug release activated by a tumor slightly acidic environment can be realized, so that drug toxicity to normal tissue cells is avoided.