69 results about "Upconversion nanoparticle" patented technology

The invention relates to the technical field of analytical chemistry, and particularly discloses a double-signal ratio type up-conversion luminescent probe, a preparation method thereof and application of the double-signal ratio type up-conversion luminescent probe in oxalate detection, and the double-signal ratio type up-conversion luminescent probe comprises up-conversion nanoparticles UCNPs and an iron-chromium cyanine R compound. The upconversion nanoparticles UCNPs are combined with the iron-chromium cyanine R compound to construct a double-emission-peak regulation and control system, when the system is used for oxalate detection, oxalate can be chelated with Fe < 3 + > in the iron-chromium cyanine R compound, blue shift of an induced absorption spectrum is achieved, green and red emission peaks of the UCNPs are synchronously regulated and controlled, and the detection sensitivity is high. The fluorescent-colorimetric bimodal high-sensitivity detection of the oxalate is realized.

The invention discloses a photo-thermal-electric coupling titanium-based implant as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The photo-thermal-electric coupling titanium-based implant provided by the invention comprises a porous titanium substrate and a hydrogel layer, the surface of the porous titanium substrate is of a TiO2 nanotube array structure; up-conversion nanoparticles and g-C3N4 nanoparticles are arranged between the porous titanium substrate and the hydrogel layer, and the up-conversion nanoparticles are NaYF4 up-conversion nanoparticles co-doped with Yb < 3 + > and Er < 3 + >; carbon nanotubes and medicinal active ingredients are loaded in the hydrogel layer. According to the titanium-based implant, collaborative coupling in light-heat-electricity multi-energy forms is achieved, the titanium-based implant is endowed with the integrated function of light response, heat conversion, electric signal conduction and local treatment, and an integrated solution with structural supporting, biological activity regulation and treatment effects is provided for bone tissue defect repair.

The invention discloses a construction method and application of NaYF4: Ln < 3 + > reversible photochromic polymer core-shell nanoparticles, and relates to the technical field of material preparation, and the technical key points are as follows: the NaYF4: Ln < 3 + > reversible photochromic polymer core-shell nanoparticles are prepared by utilizing the characteristics of luminescence concealment of rare earth doped NaYF4: Ln < 3 + > up-conversion nanoparticles (UCNPs) and dynamic fluorescence of a reversible photochromic polymer; the near-infrared / ultraviolet light dual-mode reversible photochromic fluorescent material is constructed, and the synergistic luminescence mechanism of the near-infrared / ultraviolet light dual-mode reversible photochromic fluorescent material is ascertained so as to realize the application of the near-infrared / ultraviolet light dual-mode reversible photochromic fluorescent material in multi-mode fluorescent anti-counterfeiting coding.

The invention relates to the technical field of nano materials and biomedical engineering, and discloses a dye-sensitized rare earth up-conversion luminescent nano material as well as a preparation method and application thereof. The dye-sensitized rare earth up-conversion luminescent nano-material comprises a rare earth doped up-conversion nano-particle core, an unsaturated fatty acid salt self-assembled shell layer and a near-infrared dye modified on the surface of the rare earth doped up-conversion nano-particle. The unsaturated fatty acid salt coating layer endows the material with excellent water dispersibility through a hydrophilic group; a hydrophobic chain segment and an unsaturated double bond can effectively block water and oxygen permeation and quench singlet oxygen, so that the photobleaching half-life period of the material is increased by 86.7 times or more compared with that of a conventional coating material, and the light stability is excellent; the material successfully realizes efficient up-conversion luminescence under excitation of a low-power near-infrared light emitting diode (NIR LED, the power density of which can be as low as 100mW / cm < 2 >), and thoroughly gets rid of dependence on a high-cost and high-risk laser.

The invention relates to the technical field of drug loading systems, and discloses a nanoparticle applied to photodynamic therapy and a preparation method thereof.The nanoparticle is of a core-shell-crown composite structure and sequentially comprises an inner core layer, an outer core layer, an inner shell layer and an outer shell layer from inside to outside, the inner core layer is composed of NaYF4 up-conversion nanoparticles, and the outer core layer is composed of NaYF4 up-conversion nanoparticles; the middle layer is a mesoporous Zr-MOF layer; the shell layer is a pH responsive polymer layer which is formed by connecting a polyethylene glycol-polycaprolactone block copolymer with folic acid through a hydrazone bond; the targeting canopy layer comprises double targeting ligands, and the double targeting ligands are respectively a bicyclo [6.1. 0] nonyne functionalized iRGD peptide and an azide functionalized anti-EGFR (epidermal growth factor receptor) nano antibody 7D12. The nanoparticles have the advantages of high stability, strong targeting property, high photosensitizer loading and the like, and can efficiently generate active oxygen.

The invention relates to a polysiloxane self-migration induced multi-stimulus response and programmable gradient hydrogel as well as a preparation method and application thereof. The invention relates to a hydrogel driver, and particularly discloses a polysiloxane self-migration induced gradient hydrogel driver which is quick in response, reusable, better in tensile property, multi-stimulation response and programmable in complex deformation capability. The preparation method comprises the following steps: putting a polysiloxane cross-linking agent, a temperature-sensitive monomer, spiropyrane coordinated up-conversion nanoparticles, a photoinitiator and a cosolvent in different formulas into a polytetrafluoroethylene mold, and carrying out one-step green LED polymerization to prepare the gradient hydrogel. The polysiloxane cross-linking agent is utilized to effectively promote autonomous formation of composition gradient and structure gradient in the hydrogel, and shrinkage stress change from top to bottom is caused. The hydrogel driver disclosed by the invention has the capabilities of rapid driving, reversible circulation, multiple stimulation response and programmable complex deformation, and has a wide application prospect.

The invention relates to the field of bioanalytical chemistry and environmental monitoring, and discloses a nano fluorescent probe, a preparation method and application thereof, and a method for detecting microcystic toxins in liquid. The nano composite probe comprises an MOF material and nano particles loaded on the MOF material, the nano particles comprise carboxyl-modified up-conversion nano particles and nucleic acid aptamers covalently connected with the up-conversion nano particles through amido bonds, and the up-conversion nano particles contain a thulium element and an erbium element. The nano fluorescent probe has the advantages of high specificity, simplicity and rapidness in operation, high sensitivity, high interference resistance, simple preparation process and low cost, provides an efficient solution for rapid detection of liquid microcystin pollution, and has a remarkable market prospect.

The invention discloses a rare earth homologous dual-emission high-resolution deep imaging method based on deep learning enhancement. A plurality of long-life intermediate states of lanthanide upconversion nanoparticles (UCNPs) are utilized, a single 980nm pumping source is used for exciting Tm < 3 + > / Yb < 3 + > co-doped UCNPs, and two-photon fluorescence with higher penetrability and four-photon fluorescence with higher resolution are induced at the same time. By combining a dual-mechanism CycleGAN artificial neural network based on antagonism training and cyclic consistency constraint, cross-domain mapping between two fluorescence signals is established, and the advantages of two fluorescence imaging are cooperatively utilized, so that deep penetration and high-resolution imaging are realized. According to the invention, in a self-established wide-field microscopic imaging system, the resolution of 209nm of a single particle in a 808nm fluorescence channel can be realized, and the signal-to-background ratio is doubled. According to the invention, the technical bottleneck that the resolution and the penetration depth of a traditional multi-photon microscope in deep tissue imaging are mutually restricted is effectively solved.

The invention discloses an optogenetics system for promoting targeted homing of MSC (mesenchymal stem cells) to damaged liver and a preparation method of the optogenetics system, and develops light-controlled CXCR4 release plasmids containing Uvr8 and Cxcr4 sequences and up-conversion nanoparticles capable of converting NIR (non-infrared radiation) with strong tissue penetrating power into UVB on the basis of the characteristics that UVR8 presents a dimer structure in a natural state and is dissociated after being irradiated by UVB (ultraviolet B). The method is used for realizing overexpression of CXCR4 only on an MSC cell membrane in a liver region so as to promote the targeted homing effect, and can effectively overcome the defects of high carcinogenic risk, complicated operation, low cost benefit and the like in the existing method.

The application discloses a multifunctional nanodiagnosis and treatment probe and a preparation method and application thereof, and belongs to the technical field of nanobiomedicine and tumor molecular diagnosis and treatment. The multifunctional nanodiagnosis and treatment probe is prepared by the following steps: coating mesoporous silica on the surface of a core-shell structure upconversion nanoparticle, then adsorbing platinum particles and methylene blue into the mesoporous silica, then modifying polydopamine to the surface of the mesoporous silica coated core-shell structure upconversion nanoparticle on which the platinum particles and the methylene blue are adsorbed, and finally linking nucleic acid PH1 chains and EH2 dendrites to the surface of the upconversion nanoparticle and dispersing and preparing by using a PBS buffer. The multifunctional nanodiagnosis and treatment probe prepared by the application can not only realize specific recognition, quantitative detection and in-situ imaging of a target microRNA, but also can form a synergistic tumor treatment mode of "photodynamic therapy-optical thermal therapy-oxygen enhancement", and has a wide application prospect in early tumor screening, curative effect evaluation and individualized treatment.

The application discloses a kind of near-infrared benzothiadiazole dye sensitized rare earth upconversion luminescence nano-probes and preparation method thereof;Probe is composed of near-infrared benzothiadiazole dye and upconversion nanoparticle, benzothiadiazole dye is compounded with ligand-free lanthanide ion doped upconversion nanoparticle, and upconversion luminescence is enhanced.This application preparation method is simple, and it does not involve complex core-shell structure and energy migration process, benzothiadiazole dye is directly compounded with ligand-free rare earth nanoparticle, and upconversion luminescence nano-probe is formed, under the irradiation of 808nm laser, the emission spectrum of benzothiadiazole dye and the absorption spectrum of sensitizer ion in upconversion nanoparticle overlap, which can effectively enhance the upconversion luminescence intensity of nano-probe, and keep good light stability, keep more than 90% of initial luminescence intensity under the irradiation of 120 minutes excitation light.

The invention provides an anti-tumor targeting nano material and a preparation method thereof, and belongs to the field of novel targeting nano drugs. The method comprises the following steps: stirring and mixing up-conversion nanoparticles, calcium peroxide nanoparticles, manganese chloride and a chemotherapeutic drug in a water phase to obtain a drug-loading core; cystamine and 3-aminophenylboronic acid are dissolved in water, EDC and NHS are added, a first amidation reaction is carried out, and a cross-linking agent is obtained; dispersing the drug-loading core in a polyacrylic acid solution, adding a cross-linking agent, and then carrying out a cross-linking reaction to obtain an intermediate product; activating carboxyl on the surface of the intermediate product, and then carrying out second amidation reaction with cRGD-PEG-NH2, so as to obtain the anti-tumor targeting nano material. According to the application, a multifunctional nano platform integrating targeted enrichment, double-response precise drug release, chemotherapy-chemical kinetics-immune collaborative treatment and up-conversion imaging tracing is constructed, and specific efficient killing of tumors and inhibition of metastasis and relapse of the tumors are realized.

The invention discloses a three-layer rare earth fluoride, an up-conversion nanoparticle, a tumor in-situ vaccine and application. The three-layer rare earth fluoride has a core-shell structure and comprises a core nanocrystal, a first shell layer and a second shell layer from inside to outside, wherein the crystal phase of the core nanocrystal is alpha-NaYbF4, and the core nanocrystal comprises a doping element; the doping element comprises Tm; the composition of the first shell layer comprises NaYbF4; and the composition of the second shell layer comprises NaYF4. The three-layer rare earth fluoride can realize conversion between near-infrared light and ultraviolet light. Furthermore, the up-conversion nanoparticles containing the three layers of rare earth fluorides and the photo-crosslinking agent can be used for efficiently capturing and enriching tumor antigens in situ under the near-infrared light activation condition, and excellent tumor antigen presentation efficiency is ensured; in addition, the tumor in-situ vaccine containing the three-layer rare earth fluoride, the photo-crosslinking agent and the active medicine can obviously improve the in-situ treatment effect.

The invention belongs to the technical field of environmental photocatalytic environmental governance, and particularly relates to a plasma enhanced magnetic nano composite photocatalyst with a memory catalysis effect, a preparation method and application, and the nano composite photocatalyst is a NaYF4: Yb, Tm-coated TiO2 / Fe3O4 core-shell structure nano composite material deposited by plasma enhanced magnetic Au. According to the nano composite photocatalyst, TiO2 is used as a matrix, and efficient conversion from visible light to ultraviolet light is realized by coating NaYF4: Yb, Tm up-conversion nanoparticles capable of being excited by near-infrared light; fe3O4 is compounded to endow the material with magnetic recovery performance; au nano-particles deposited on the surface of the core shell enhance the surface plasma effect and carrier separation, and the photocatalytic activity is further improved. The plasma enhanced magnetic nano-composite photocatalyst with the memory catalysis effect can efficiently degrade organic pollutants under the illumination condition, can perform continuous catalytic degradation after illumination is stopped, and provides a breakthrough technical path for solving the problem of sustainable degradation of the current photocatalysis technology under the complex environment condition.

The present invention discloses an upconversion composite nanomaterial for detecting triclosan, its preparation method and application, and belongs to the technical field of triclosan detection. The upconversion composite nanomaterial provided by the present invention comprises carbon dots, upconversion nanoparticles and ruthenium salt; the upconversion nanoparticles are core-shell structures, which are NaYF4:Yb 3+ / Er 3+ The nanoparticles are the core, and the surface of the core is coated with NaYF4:Eu 3+ The carbon dots and upconversion nanoparticles are connected via an amide bond. The upconversion composite nanomaterial provided by the present invention is low-cost, has a relatively simple preparation method, and exhibits high fluorescence emission intensity. It can be used to detect triclosan concentration in a simple, convenient, and low-cost manner, while enabling on-site detection and real-time monitoring.

The present invention discloses a method for detecting antibiotics based on an upconversion nanoparticle - silk fibroin nanoprobe system. The method includes preparing upconversion nanoparticles synthesized from lanthanide elements, coating water - soluble silica on the upconversion nanoparticles, followed by washing, collecting, and vacuum drying. Fibrous silk fibroin is dissolved, filtered, dialyzed, and concentrated to obtain an aqueous solution of silk fibroin. An antibiotic and a chromogenic agent are mixed to obtain an aqueous solution of a chromogenic agent - antibiotic complex. The upconversion nanoparticles, the aqueous solution of silk fibroin, and the aqueous solution of the chromogenic agent - antibiotic complex are mixed to obtain upconversion nanoparticles coated with silk fibroin and antibiotics. The obtained upconversion nanoparticles are deionized and recovered, and then the fluorescence intensity is measured. The concentration of the antibiotic to be detected is judged according to the fluorescence intensity to achieve the detection of antibiotics. The process of the present invention is simple, easy to operate, low in cost, and high in sensitivity, and has broad application prospects in the fields of quantitative detection of pollutants, antibiotic drugs, etc.

The invention discloses a precise construction strategy of a high-sensitivity DNA circulation inducible fluorescent nanorobot, which specifically comprises the following steps: A1, constructing a DNA nanostructure framework which is composed of a modularly designed recognition domain, a signal conversion domain and a circulation trigger domain, the recognition domain comprises an aptamer or a hairpin structure and is used for specifically combining with a target molecule, and the circulation trigger domain is used for specifically combining with the target molecule; the invention relates to the technical field of gene engineering. According to the precise construction strategy for the high-sensitivity DNA circulating inducible fluorescent nano-robot, through integration of a modular DNA tetrahedral framework and an enzyme digestion-strand displacement synergistic amplification system, cyclic utilization and signal exponential gain (such as CHA cascade amplification combined with CRISPR-Cas12a trans-cleavage) of a target object are achieved, the detection limit breaks through the 0.1 am level, and the accuracy of the high-sensitivity DNA circulating inducible fluorescent nano-robot is greatly improved, so that the accuracy of the high-sensitivity DNA circulating inducible fluorescent nano-robot is improved, and the accuracy of the high-sensitivity DNA circulating inducible fluorescent nano-robot is improved. Compared with a traditional single enzyme digestion or linear amplification method, the sensitivity is improved by at least three orders of magnitude. Meanwhile, time-resolved upconversion nanoparticles (UCNPs) are combined with a magnetic bead separation-lock type probe noise reduction technology.

The present invention discloses a white-light-emitting upconversion rare-earth-doped nanomaterial and a preparation method thereof. The core-shell structured LiYF4:xHo@LiYbF4@LiYF4 upconversion nanoparticles (0 < x ≤ 0.02) with a particle size of 28-38 nm are synthesized by the high-temperature co-precipitation method. They have good dispersibility and obvious upconversion luminescence. Under 980 nm laser excitation, they exhibit upconversion fluorescence at 480 nm, 540 nm and 650 nm, making their overall color show white light, and having good application prospects in the fields of biomedicine, solar cells, temperature sensors and photocatalytic degradation, etc.

The present invention discloses a reversible photoelectrochemical microsensor based on optical regulation. A reversibly identifiable small molecule probe is introduced into the microsensor as the recognition unit of the photoelectrochemical microsensor and a regulator of fluorescence resonance energy transfer efficiency. The small molecule probe acts as an energy acceptor and combines with energy donor upconversion nanoparticles through the principle of fluorescence resonance energy transfer (FRET) to form a fluorescent probe. The luminescence of the FRET fluorescent probe serves as the excitation light source of the reversible photoelectrochemical microsensor. The reversible response of the small molecule probe to the target SO2 is utilized to regulate the fluorescence emission intensity of the FRET probe, thereby achieving reversible output of the photoelectric signal. The present invention achieves reversible detection, which is currently unattainable in photoelectrochemical sensors due to the limitations of the response mechanism of the recognition element. Furthermore, due to the introduction of a small molecule probe with highly selective chemical recognition as the recognition element, the photoelectrochemical microsensor proposed by the present invention has higher specificity than the recognition element of traditional photoelectrochemical sensors and can detect non-electroactive substances.

The application belongs to the technical field of nanometer luminescent materials, and particularly relates to a near-infrared light response upconversion nanocomposite material, a preparation method and application thereof. The nanocomposite material comprises a rare earth upconversion nanoparticle inner core, a porphyrin metal-organic framework shell layer, a near-infrared dye loaded in the porphyrin metal-organic framework pores, and an amphiphilic phospholipid polymer modified on the outer surface of the porphyrin metal-organic framework. The metal ion in the porphyrin metal-organic framework shell layer is a metal ion with a heavy atom effect, and the metal ion is selected from at least one of Pt(II), Pd(II), Cu(II), Mn(II) or Ir(Ⅳ). The nanocomposite material has excellent singlet oxygen production and photodynamic sterilization effect.

The present invention provides an upconversion luminescent material and its preparation method and application, which belongs to the field of luminescent materials. The present invention provides an upconversion luminescent material, which is a core-shell structure, wherein the core is Ho 3+ Ion doping AR2(BO4)4 or Ho 3+ Ion doped ABO4, shell is Yb 3+ Ion doping AR2(BO4)4 or Yb 3+ Ion doping ABO4; A is a divalent transition metal ion or a divalent main group metal ion; R is a trivalent rare earth metal ion; B is Mo 6+ or W 6+ The present invention converts Yb 3+ ions are doped into the matrix material and encapsulated with Ho 3+ The core layer doped with luminescent center ions increases the distance between the luminescent center and the surface defects of the upconversion nanoparticles, reducing the influence of surface defects and surface organic molecular vibration on upconversion luminescence; at the same time, the Yb 3+ The shell layer enhances the absorption of excitation light by the upconversion luminescent material.

The application discloses a kind of lysosome-targeting protein modulators based on upconversion nanomaterial-DNA nanostructure and preparation method thereof.The lysosome-targeting protein modulators are combined by covalent bond from carboxyl functionalized upconversion nanoparticles and amino-modified DNA nanostructure, mainly enter cancer cells through folate receptor, after near infrared light illumination and glutathione response, the target protein combined is transported into lysosome and degraded.The lysosome-targeting protein modulators of the application have the advantages of light affinity, high controllability and storage stability, are easy to be metabolized by organism, and have good application prospect in the field of cell biology and cancer targeted therapy.

The invention relates to the technical field of medical implantable optical and electrical integrated devices, in particular to a composite functional degradable optical fiber and a preparation method and application thereof. The composite functional degradable optical fiber provided by the invention has the characteristics of biodegradability and high flexibility, permanent foreign body reaction can be avoided, and the biocompatibility is greatly improved. According to the composite functional degradable optical fiber provided by the invention, based on the unique physical characteristics of the up-conversion nanoparticles, the inherent synergistic treatment of PBM and PDT under the driving of a single near-infrared light source is realized. According to the composite function degradable optical fiber provided by the invention, a treatment-feedback closed-loop system architecture of minimally invasive monitoring is integrated, and a good hardware basis is provided for realizing self-adaptive treatment. The composite functional degradable optical fiber provided by the invention can be applied to the field of medical implantation instruments, the field of neural engineering or the field of biological materials, and particularly can be applied to brain implantation materials or brain degenerative disease self-adaptive treatment systems.

The invention provides nanoparticles for tumor photodynamic therapy and a preparation method, a pharmaceutical composition and application thereof, and belongs to the technical field of nano biomedicine. The nanoparticle for tumor photodynamic therapy comprises: an up-conversion nanoparticle; the phospholipid layer is used for coating the up-conversion nano-particles; the nucleic acid aptamer and the endoplasmic reticulum targeting peptide are modified on the surface of the phospholipid layer; and a photosensitizer supported on the surface of the nanoparticle. The nucleic acid aptamer and the endoplasmic reticulum targeting peptide are further co-modified on the basis of the surface of the upconversion nano-particle subjected to phospholipidation treatment, so that the nano-particle can actively recognize tumor cells and deliver the tumor cells to the endoplasmic reticulum, and dual targeting on the tumor cells is realized. The loaded photosensitizer can generate reactive oxygen species (ROS) under excitation of near-infrared light, endoplasmic reticulum stress is specifically induced, then a cell apoptosis pathway is activated, and tumor cells are killed.

The invention provides a high-transparency infrared contact lens and a preparation method thereof, and belongs to the technical field of infrared contact lenses. The high-transparency infrared contact lens is prepared from the following raw materials: organic hydrogel and modified up-conversion nanoparticles, the mass ratio of the organic hydrogel to the modified upconversion nanoparticles is 100: (3-8); the organic hydrogel is poly (2-hydroxyethyl methacrylate) hydrogel or silicon-based hydrogel; according to the invention, a specific type of organic hydrogel is combined with the modified up-conversion nanoparticles, so that near-infrared light invisible to naked eyes can be converted into visible light, and near-infrared light detection and sensing functions are realized; the light transmittance and transparency are good, and the visible light transmittance is not influenced.

This invention relates to the field of drug delivery systems, and discloses a nanoparticle for photodynamic therapy and its preparation method. The nanoparticle has a core-shell-corona composite structure, comprising, from the inside out: a core layer composed of NaYF4 upconversion nanoparticles; a middle layer: a mesoporous Zr-MOF layer; a shell layer: a pH-responsive polymer layer formed by linking folic acid to polyethylene glycol-polycaprolactone block copolymer via hydrazone bonds; and a targeting corona layer comprising dual targeting ligands, namely a bicyclic [6.1.0]nonyne-functionalized iRGD peptide and an azide-functionalized anti-EGFR nanobody 7D12. The nanoparticles of this invention possess advantages such as high stability, strong targeting, and high photosensitizer loading, and can efficiently generate reactive oxygen species.

The invention discloses hydrogel for osteoporotic bone defect repair and a preparation method of the hydrogel. The hydrogel comprises basic hydrogel and mineralized photosynthetic microorganisms, the mineralized photosynthetic microorganism comprises a photosynthetic microorganism, a mineralized shell layer arranged on the surface of the photosynthetic microorganism, and rare earth up-conversion nano-particles loaded in cells of the photosynthetic microorganism, and the rare earth up-conversion nano-particles can convert near-infrared light into visible light. According to the present invention, the oxygen production type mineralized microalgae composite hydrogel with characteristics of deep tissue activation, controllable oxygen production and simultaneous realization of four-effect synergy of acid neutralization, continuous oxygen supply, angiogenesis promotion and osteogenesis mineralization can be provided, such that osteoblast activity can be promoted, osteoclast activity can be inhibited, in-vivo osteogenesis can be promoted, and the technical support can be provided for osteoporotic bone defect repair.

The invention discloses an up-conversion fluorescent probe, an isoniazide detection method and a portable isoniazide detection sensor, and belongs to the field of biological detection. The up-conversion fluorescent probe is composed of lanthanide doped core-shell structure up-conversion nanoparticles subjected to acid pickling treatment and p-dimethylaminobenzaldehyde, the core particle size of the up-conversion nanoparticles ranges from 25.85 nm to 28.85 nm, and the thickness of a shell layer ranges from 0.98 nm to 1.38 nm. The lanthanide doped core-shell structure up-conversion nanoparticles are used as energy donors, p-dimethylaminobenzaldehyde is used as a specific recognition molecule, and ratio-type fluorescence detection is realized through an inner filter effect. Isoniazide reacts with DMAAB to generate Schiff base, so that fluorescence at 475 nm is quenched while fluorescence at 655 nm is unchanged, and the color of the fluorescence is changed from blue to red. Due to the near-infrared excitation characteristic of the up-conversion nanoparticles, the up-conversion nanoparticles are not easily interfered by background fluorescence, so that the rapid quantitative detection of isoniazide in whole blood is realized, and the detection limit is as low as 2.4 mu m. The method is easy and convenient to operate, high in anti-interference performance and suitable for clinical individualized medication monitoring.

The application provides a kind of molecular self-assembly triplet-triplet annihilation (TTA) photonic upconversion hydrogel and its preparation method and application, select polyethylene oxide-polypropylene oxide-polyethylene oxide (PEO-PPO-PEO) F127 three-block polymer to encapsulate upconversion dye pair, form nanomicelles.Utilize three-block polymer hydrophilic section PEO and hydrophobic section PPO in aqueous solution self-assembly to form nanoparticles, hydrophilic end is exposed outside, form dense oxygen barrier shell, hydrophobic end and hydrophobic luminescent material in core, avoid being quenched by oxygen in air.Again, add aqueous solution containing upconversion nanoparticles into acrylamide aqueous solution, directly polymerize, obtain upconversion hydrogel.The strategy is not only simple operation, without complex modification to luminescent material, and does not contain volatile organic solvent, and polyacrylamide hydrogel also has good biocompatibility, so it has great application prospect in biological field.

This invention discloses a method for preparing and applying nanochloroplasts with targeted mitochondrial repair function, belonging to the field of biomedical technology. The preparation method includes the following steps: mixing energy module nanoparticles and catalytic module nanoparticles with a cell membrane solution, and co-encapsulating them in a compartmentalized manner using membrane extrusion technology to form a nanochloroplast core; then, co-modifying the surface of the nanochloroplast core with upconversion nanoparticles through asymmetric modification. The nanochloroplasts of this invention can simulate the partitioned structure of natural chloroplasts, achieving phototaxis, energy supply, and carbon fixation functions, and can target mitochondria for restoring mitochondrial metabolism. This system can reconstruct the mitochondrial oxygen-carbon metabolism network in an ischemic model and is expected to develop into a novel nanomaterial for more effective treatment of ischemic diseases.