90 results about "Upconversion nanoparticle" patented technology

The invention provides 'core-shell-shell' rare earth upconversion nanoparticles (UCNPs) using Nd as a sensitizing agent. The rare earth UCNPs are characterized in that the shell layer of the rare earth UCNPs contains Nd<3+> and each layer contains Yb<3+>. The special microscopic nanostructure solves the problem about the quenching effect on upconversion fluorescence in an Nd doping process, so bright upconversion fluorescence can be obtained. Compared with the traditional Yb-sensitized UCNPs, the Nd-sensitized UCNPs have higher infrared absorption intensity so as to greatly improve upconversion efficiency and fluorescence. The Nd-sensitized UCNPs adopt 800-nanometer instead of 980-nanometer laser as an exciting light source, so the Nd-sensitized UCNPs have smaller overheating effect and stronger biological tissue penetrating power. Therefore, the novel rare earth upconversion material is expected to be widely applied in the field of biomedicines.

The present invention relates to rare earth upconversion nanoparticles, a preparation method and uses thereof, wherein the rare earth upconversion nanoparticles sequentially comprise an activator shell layer, an energy transfer agent shell layer and a sensitizing agent shell layer from inside to outside, each layer contains Yb<3+>, and the doping concentration of Yb<3+> is gradually reduced from inside to outside. According to the present invention, the ytterbium ion doping concentration gradient is set to effectively transfer the excitation state energy of the sensitizing agent to the activator, such that the upconversion luminescence quantum yield of the rare earth upconversion nanoparticles is effectively improved and achieves 0.22%, and the 800 nm continuous laser excitation at the excitation light source intensity of 2W/cm<2> is achieved; and the rare earth upconversion nanoparticles can produce strong upconversion luminescence under the infrared LED light source excitation, and the potable detection equipment based on the rare earth upconversion nanoparticle luminescence probe can be designed by using the unique packaging and array technology of the infrared LED light source so as to further expand the application of the upconversion luminescence material in the biomedical field.

The invention relates to a microarray design of hybrid upconversion nanoparticles on a nanoporous anodized alumina membrane heterogeneous assay for simultaneous detection of multiple oligonucleotides, for example, oligonucleotides from different types of viruses.

The invention discloses an internally and externally refined composite nano photosensitizer as well as a preparation method and application thereof and belongs to the technical field of nanometer biomedicine. The preparation method disclosed by the invention comprises the following steps: removing a surface ligand from rare-earth upconversion nanoparticles, and modifying by a photosensitizer to obtain an upconversion nano photosensitizer; and coating luminescence molecules and the upconversion nano photosensitizer by virtue of hydrophobic-hydrophobic interactions by adopting amphiphilic polymers, thereby obtaining the composite nano photosensitizer. The composite nano photosensitizer is capable of simultaneously responding to stimulus of a tumor microenvironment so as to realize internal photodynamic force and external photodynamic force under near-infrared light excitation. By combining the characteristic that hydrogen peroxide and the like is highly expressed in the tumor microenvironment with the characteristic of upconversion emission of near-infrared light excitation, tumors are inhibited in both modes in an internally and externally refined manner. After a covalent or chemical coordination manner on the surface of the rare-earth upconversion nanoparticles is combined with the photosensitizer, the energy transfer efficiency can be improved, the singlet oxygen yield is increased, and the photodynamics therapy effect under external excitation can be effectively improved.

The invention discloses a method for preparing a nanometer system for multi-model diagnosis and treatment integration. The method comprises the following steps: adopting an in-situ growth nanogold modified rare-earth upconversion nanoparticle method to perform ligand exchange modification on oil-soluble upconversion luminescence nanoparticles of citric acid with a core-shell structure to be water-soluble, and performing citric acid ligand modification on the surfaces of the nanoparticles; uniformly growing nanogold particles on the surface through an in-situ crystal growth method so as to obtain in-situ growth nanogold modified upconversion luminescence nanoparticles; and linking polyethylene glycol-adriamycin hydrochloride containing hydrazone bonds and thiol onto the nanogold surface, thereby obtaining the rare-earth upconversion nanometer system. The invention further provides the nanometer system and application thereof. The nanometer system provided by the invention is uniform in size, high in stability and high in biocompatibility, has the effects of chemotherapy and photo-thermal physiotherapy and can be applied to the fields of upconversion fluorescence imaging, magnetic resonance imaging, anti-cancer drug delivery, photo-thermal physiotherapy of cancers and the like.

The invention relates to a fluorescent bioprobe for upconverting nanoparticle-labeled aptamers, which belongs to the technical field of biomolecular detection. In order to overcome the technical problems of existing QDs-labeled aptamer biological probes, including damage to the nucleic acid of the organism where the target molecule is located, high background signal of QDs, potential toxicity, "blinking" effect and chemical instability, provide A fluorescent bioprobe for up-conversion nanoparticle-labeled aptamers, including aptamers labeled with up-conversion nanoparticles as fluorescent donors, and DNA strands complementary to the aptamers, which are connected with fluorescent The donor undergoes a FRET effect as the fluorescent acceptor dye molecule. The fluorescent donors used in the present invention are UCNPs, which have the advantages of low background signal, non-toxicity, no "blinking" effect and strong chemical stability.

The invention provides a preparation method for upconversion nanoparticles wrapped by mesoporous silica loading indocyanine green. Upconversion is wrapped by mesoporous silica, and the biosecurity ofthe particles and the loading capacity of the indocyanine green are improved. The size ofthe prepared upconversion nanoparticles wrapped by the mesoporous silica loading the indocyanine green is 80-400 nanometers; after the upconversion nanoparticles wrapped by the mesoporous silica loading the indocyanine green (ICG) are added, the survival rate of cells is within the range of 85-95%; a confocalmicroscope can observe cytophagy of the particles in the cells;after particle addition through laser radiation, the death rate of the cells is within the range of 40-75%.

The invention provides a nano carrier for near-infrared light triggered release of a chemotherapy drug and a preparation method thereof. The preparation method comprises the steps: polymerizing a photosensitive monomer, a hydrophilic monomer and a hydrophobic monomer, to prepare a photosensitive polymer; providing oleic acid modified upconversion nanoparticles, mixing the photosensitive polymer with the upconversion nanoparticles, and allowing the photosensitive polymer to act on the upconversion nanoparticles, to prepare the nano carrier. The nano carrier for near-infrared light triggered release of the chemotherapy drug is composite upconversion nanoparticles having good hydrophilia, and has a structure with a single-layer shell wrapping multiple cores, so that the nanoparticles can quickly response to near-infrared light and accurately release the drug in vivo, and the problem of low release efficiency of general drug loaded nanoparticles is overcome.

The invention belongs to the field of micromolecular detection, and relates to a method and a kit for fluorescence detection of micromolecular mycotoxin based on a metal organic framework and upconversion nanoparticles. The method comprises the following steps: S1, obtaining an up-conversion nanoparticle probe modified with a micromolecular mycotoxin aptamer; S2, carrying out synthesis and activation of MIL-101 (Cr); S3, combining the micromolecular mycotoxin with the aptamer; and S4, carrying out signal detection: detecting a fluorescence signal of a product obtained by the reaction in the step S3 by adopting a fluorospectro photometer. The upconversion nanoparticles doped with rare earth are adopted, the fluorescence intensity is stable, the background value is low, and compared with other methods, the fluorescence detection kit does not need complex operation steps, is high in applicability, high in detection result sensitivity and good in specificity, and can be applied to rapid detection of on-site samples.

The invention provides a method for preparing a clenbuterol hydrochloride molecularly imprinted membrane electrochemical light emitting sensor. The method is characterized in that the surface of a glassy carbon electrode is wrapped by a composite film, and the composite film consists of reduced oxidized graphene, upconversion nanoparticles and molecular imprinting polymers. The method has the advantages that electrochemical light emission of the upconversion nanoparticles with stable cathode signals and high light emission strength are combined with the molecular imprinting polymers with goodselectivity, and trace detection on clenbuterol hydrochloride in animal derived foods can be implemented.

The invention relates to a preparation method and application of a gold-upconversion nanoparticle tripolymer, belonging to the technical field of analytical chemistry. The preparation method mainly comprises the following steps: synthesis of gold nanoparticles with the particle size of 20nm, modification of Mucin-1 aptamer on gold nanoparticles, modification of AFP aptamer on upconversion nanoparticles, modification of AFP and Mucin-1 aptamer partial complementary sequences on gold nanoparticles, assembly of gold-upconversion nanoparticle tripolymer, and fluorescent and Raman detection. After the AFP is applied, the fluorescence can be enhanced; and after the Mucin-1 is applied, the Raman signal becomes weak, thereby establishing the standard curve between fluorescent signals and AFP concentration and the standard curve between Raman signals and Mucin-1 concentration. The invention provides a multiplex supersensitive detection method for cancer biomarkers by using the gold-upconversion nanoparticle tripolymer. Compared with the traditional detection methods, the multiplex supersensitive detection method has the advantages of low cost and high sensitivity, is convenient and quick, and has favorable practical application prospects.

The invention discloses a fluorescence detection test strip for simultaneously detecting four types of pathogenic bacteria, and a preparation method and application thereof. The bottom layer of the test strip is a supporting plate; the supporting plate is pasted with a sample cushion, a combination cushion, a nitrocellulose membrane and an adsorption cushion in sequence, wherein the sample cushion, the combination cushion, the nitrocellulose membrane and the adsorption cushion are tightly connected; the nitrocellulose membrane is provided with four detection lines and one quality control linein sequence; the combination cushion is coated with an upconversion nanoparticle-pathogenic bacteria first aptamer compound; the detection line is independently coated with a pathogenic bacteria second aptamer-streptavidin compound, i.e., a capture probe; and the quality control line is coated with a polyT-sequence-streptavidin compound, i.e., a quality control probe. The test strip has the advantages of high sensitivity, good specificity and low cost, can realize the simultaneous detection of four types of pathogenic bacteria in a complex sample, and has a good application prospect.

The invention discloses a preparation method of a rare earth upconversion nanoparticle/bismuth vanadate nano composite material with an antitumor effect, and relates to a synthesis method of an antitumor nano material. The invention aims to solve the problems that BiVO4 synthesized by the existing method is large in size and the application of BiVO4 in the field of biomedicine is limited. The preparation method comprises the following steps: 1, preparing synthetic core structure nanoparticles; 2, synthesizing core-shell structure nanoparticles; 3, preparing hydrophilic up-conversion nanoparticles; 4, synthesizing zinc nitrophthalocyanine; 5, synthesizing amino zinc phthalocyanine; 6, synthesizing a BiVO4 nanosheet by a high-temperature pyrolysis method; 7, connecting UCNPs with zinc aminophthalocyanine; and 8, preparing and modifying the composite material PVP/UCNPs-ZnPc@BiVO4. The preparation method is used for preparing the rare earth upconversion nanoparticle/bismuth vanadate nano composite material with the antitumor effect.

The invention discloses a rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material. The rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material is acuprous oxide modified rare earth upconversion nanocomposite prepared by the following steps: using oil-soluble upconversion nanoparticles (UCNPs) as cores; and then, allowing epitaxial growth of cuprous oxide (Cu(2)O) on surfaces of the UCNPs so as to form shells, thereby obtaining the cuprous oxide modified rare earth upconversion nanocomposite grown by interface nucleation. The Cu(2)O can transfer fluorescence resonance energy with upconversion emission of the UCNPs excited by near-infrared light so as to produce sufficient active oxygen, thereby meeting needs of photodynamic therapy; and moreover, the Cu(2)O can synchronously cooperate with the oil-soluble UCNPs so as to realize integrated diagnosis and treatment. The invention further provides a preparation method of the rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material. The preparation method of the rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material comprises the following steps: using the oil-soluble UCNPs as cores; and then, allowing interface nucleation growth of the (Cu(2)O) on the surfaces of the UCNPs. The rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material prepared by the preparation method is uniform in size, good in stability, and excellent in biocompatibility; and thus, the nanocomposite material can be used in imaging diagnosis guided photodynamic therapy so as to meet needs of clinical diagnosis and treatment integration.

The invention discloses a preparation method of a GdF3:Yb3+ and Er3+ to upconversion fluorescence nanomaterial. The preparation method comprises the following steps that (a) anhydrous ethanol, deionized water and a thiohydracrylic acid solution are mixed; (b) Gd(NO3)3.6H2O and Yb(NO3)3.5H2O powder is weighed and put in the mixed solution, and then a Er(NO3)3.5H2O solution is added; (c) an NH4F solid is weighed and added into the mixed solution in the step (b); (d) the liquid prepared in the step (c) is transferred to a polytetrafluoroethylene hydrothermal high-pressure reaction kettle, and continuous heating is performed at high temperature; (e) after the reaction kettle is cooled to room temperature, centrifugation is performed, and a precipitate is washed with ionized water and anhydrous ethanol and is dissolved in the deionized water; (f) the solution obtained in the step (e) is centrifuged, and the white precipitate is dried. Thiohydracrylic acid is used as a surface modification agent, GdF3:Yb3+ and Er3+ to upconversion nanoparticles are synthesized by adopting a one-step hydrothermal method, the varieties of modification agents for surface modification of upconversion nanoparticles are enriched, and a theoretical and experimental basis is provided for further probe establishment and targeted delivery of anti-cancer drugs.

The invention discloses an up-conversion luminescent marker based on an up-conversion luminescent marker, a protein chip and a detection method. The array of biologically active molecules on a glass slide or other carrier medium has a detection antibody fixed at the detection site, which can detect It specifically binds to the target antigen of the immune complex, and the quality inspection antibody is immobilized on the quality inspection site, which will specifically bind to the antigen of the quality inspection substance in the pretreatment solution; the excitation device of the line scan is a near-infrared light source. The dichroic mirror is irradiated on the protein chip, and the detection device of the line scanning technology is a line array camera, which simultaneously collects the visible light intensity signals emitted by each point on the line. The UCNP of the present invention cross-links bioactive molecules in a covalent manner, which improves the reliability and stability of the system on the premise of ensuring the detection sensitivity.

The invention discloses a preparation method of Ca2YREF7@TiO2 nanocomposite powder for inhibiting bacteria and cancer, relates to an antitumor medicament, in particular to a method of synthesizing titanium dioxide-coated rare earth ion-doped yttrium calcium fluoride composite nanopowder by a two-step hydrothermal method. The method comprises the following steps of preparing rare earth ion-doped yttrium calcium fluoride upconversion nanoparticle powder; preparing Ca2YREF7@TiO2 nanocomposite powder with a core-shell structure. According to the preparation method disclosed by the invention, the defects that the upconversion efficiency is reduced, a preparation process is troublesome, the production period is prolonged, and the cost is increased existing in the method of preparing upconversion titanium dioxide composite powder in the prior art are overcome.

The invention discloses a self-cleaning electrode for electrochemical biosensing. The electrode comprises a conductive substrate, wherein reduced graphite oxide and photosensitizer-upconversion nanoparticle composite particles are sequentially deposited on the surface of the conductive substrate; the photosensitizer-upconversion nanoparticle composite particles comprise upconversion nanoparticles,the outer surfaces of the upconversion nanoparticles are sequentially coated with dense silicon dioxide layers and mesoporous silicon dioxide layers, and photosensitizer molecules are adsorbed in mesopores of the mesoporous silicon dioxide layers. When the self-cleaning electrode for electrochemical biosensing is irradiated by near infrared rays with high transmittance, visible light can be emitted by the upconversion nanoparticles in the photosensitizer-upconversion nanoparticle composite particles deposited on the surface to activate the photosensitizer molecules to generate oxygen free radicals with high reactivity to implement in-situ self-cleaning of the electrode. Moreover, during cleaning, the electrode is irradiated by virtue of the near infrared rays with high transmittance, so that the electrode is not required to be repeatedly disassembled and assembled, and damage to an organism is prevented.

The invention discloses a method for detecting HE4 based on fluorescence resonance energy transfer of an up-conversion nano material and a BHQ1 quenching group. The preparation method comprises the following steps of synthesizing polyacrylic acid modified up-conversion nanoparticles by a solvothermal method, and coupling and immobilizing an aptamer of which the 5 '-end is modified with amino and the 3'-end is modified with BHQ1 onto the surfaces of the particles by amidation reaction to construct an up-conversion nanoparticle probe. As fluorescence resonance energy transfer can occur between the BHQ1 and the up-conversion nanoparticles, fluorescence of the up-conversion nanoparticles can be quenched by the BHQ1; after the target object is added, affinity between the target object and the aptamer is stronger, the distance between an energy donor and an energy receptor is increased, fluorescence resonance energy transfer is blocked, and then fluorescence of the up-conversion nanoparticles is recovered; quantitative determination of the tumor marker HE4 can be realized according to the fluorescence intensity recovery degree of the upconversion nanoparticles under different concentrations of a target object. The method disclosed by the invention is simple and convenient to operate, high in sensitivity and strong in specificity, and is expected to be used for early diagnosis of ovarian malignant tumors.