Method for detecting content of mycotoxins in wheat

A mycotoxin and detection method technology, which is applied in the field of analytical chemistry, can solve problems such as failure to achieve ideal control effects and serious threat of disease epidemics, and achieve the effects of increased sensitivity and accurate toxin content
CN102401820AInactive Publication Date: 2012-04-04JIANGSU ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU ACAD OF AGRI SCI
Publication Date
2012-04-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for detecting the content of mycotoxins in wheat. The mycotoxins in the wheat are detected by utilizing high performance liquid chromatography-tandem mass spectrometry, and the mycotoxins related to the detection are deoxynivalenol (DON), 3-acetyl (Ac)-DON, 15-Ac-DON, zearalenone (ZEN) and T-2 respectively. The detection method specifically comprises the following steps of: crushing wheat samples by using a crusher, and obtaining coarse extract by using an extracting solution; obtaining a purified toxin extracting solution by using an amino column; preparing mixed toxin standard liquid with different content; detecting each mycotoxin in the toxin extracting solution by adopting the high performance liquid chromatography-tandem mass spectrometry; and obtaining a regression equation of mycotoxin content relative to a peak area according to detection results of the mixed toxin standard liquid, and calculating the content of the DON, the 3-Ac-DON, the 15-Ac-DON, the ZEN and the T-2 in the wheat sample.
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Description

technical field

[0001] The invention belongs to the field of analytical chemistry, and specifically relates to a detection method for multiple toxins in wheat, especially a detection method for DON, 3-Ac-DON, 15-Ac-DON, ZEN and T-2 toxins in wheat. Background technique

[0002] Wheat head blight is an important plant disease in my country's wheat producing areas. It not only causes serious yield loss, but also produces many mycotoxins in grains due to the pathogen Fusarium graminearum, which seriously affects the health of humans and animals. Such toxins mainly include deoxynivalenol (DON), 3Ac-DON, 15Ac-DON, T-2 and zearalenone (ZEN). The research on the control of scab in my country has gone through decades. Although important achievements have been made in pesticide control and disease-resistant breeding, the ideal control effect has not been achieved. Especially in the Huanghuai River Basin, the main wheat producing area in my country, wheat varieties are absolutely Mos...

Claims

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