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Method for detecting content of mycotoxins in wheat

A mycotoxin and detection method technology, which is applied in the field of analytical chemistry, can solve problems such as failure to achieve ideal control effects and serious threat of disease epidemics, and achieve the effects of increased sensitivity and accurate toxin content

Inactive Publication Date: 2012-04-04
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The research on the control of scab in my country has gone through decades. Although important achievements have been made in pesticide control and disease-resistant breeding, the ideal control effect has not been achieved. Especially in the Huanghuai River Basin, the main wheat producing area in my country, wheat varieties are absolutely Most of them are sensitive to scab, and the epidemic threat is serious

Method used

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  • Method for detecting content of mycotoxins in wheat
  • Method for detecting content of mycotoxins in wheat
  • Method for detecting content of mycotoxins in wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The content of DON toxin in wheat samples was determined by the national standard GB / T 23503-2009 Determination of deoxynivalenol in food - immunoaffinity chromatography purification high performance liquid chromatography.

[0026] Grind the wheat sample with a high-speed universal grinder and pass it through a 1mm aperture test sieve. Weigh 25.00 g of the sample into a 100 mL volumetric flask. The experiment is repeated five times. Add 5.0 g of polyethylene glycol respectively, and dilute to the mark with water , mix well, transfer to a homogenizing cup, stir at high speed for 2 min, filter with quantitative filter paper and then filter with glass fiber filter paper until clarified, collect the filtrate, pipette 2 mL of the filtrate and add it to the DON toxin immunoaffinity column, add 1 drop Pass the immunoaffinity column at a flow rate of / s, wash the immunoaffinity column successively with 5 mLPBS and 5 mL water until the small column is drained;

[0027] Add 1.0 m...

Embodiment 2

[0031] The content of T-2 toxin in wheat samples was determined by the national standard GB / T 23501-2009 Determination of T-2 toxin in food - immunoaffinity chromatography purification high performance liquid chromatography.

[0032] Grind the wheat sample with a high-speed universal grinder and pass it through a 1mm aperture test sieve. Weigh 25.00 g of the sample into a 100 mL volumetric flask. The experiment is repeated five times. Extraction solution: methanol + water (volume ratio of 80:20 ) water to the mark, mix well, transfer to a homogenizing cup, stir at high speed for 2 min, filter with quantitative filter paper, pipette 10.0 mL of filtrate into a 50 mL volumetric flask, add water to make up to the mark, and filter with glass fiber filter paper to Clarify, collect the filtrate, add 10 mL of the filtrate to the T-2 toxin immunoaffinity column, pass through the immunoaffinity column at a flow rate of 1 drop / s, rinse the immunoaffinity column with 10 mL of water until t...

Embodiment 3

[0037] The content of ZEN toxin in wheat samples was detected by GB / T 23504-2009 Determination of Zearalenone in Foodstuffs-Immunoaffinity Chromatography Purification High Performance Liquid Chromatography.

[0038] Grind the wheat sample with a high-speed universal grinder and pass it through a 1mm aperture test sieve. Weigh 50.00 g of the sample into a 100 mL volumetric flask. The experiment is repeated five times. Add 5 g of sodium chloride and use the extraction solution: acetonitrile + water (Volume ratio is 90:10) Dilute to the mark, mix well, transfer to a homogenizing cup, stir at high speed for 2 min, filter with quantitative filter paper, pipette 10.0 mL of filtrate into a 50 mL volumetric flask, add water to make up to the mark, After mixing, filter with glass fiber filter paper until clear, collect the filtrate, add 10 mL of the filtrate to the ZEN toxin immunoaffinity column, wash the immunoaffinity column with 10 mL of PBS washing buffer and 10 mL of water in turn...

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Abstract

The invention relates to a method for detecting the content of mycotoxins in wheat. The mycotoxins in the wheat are detected by utilizing high performance liquid chromatography-tandem mass spectrometry, and the mycotoxins related to the detection are deoxynivalenol (DON), 3-acetyl (Ac)-DON, 15-Ac-DON, zearalenone (ZEN) and T-2 respectively. The detection method specifically comprises the following steps of: crushing wheat samples by using a crusher, and obtaining coarse extract by using an extracting solution; obtaining a purified toxin extracting solution by using an amino column; preparing mixed toxin standard liquid with different content; detecting each mycotoxin in the toxin extracting solution by adopting the high performance liquid chromatography-tandem mass spectrometry; and obtaining a regression equation of mycotoxin content relative to a peak area according to detection results of the mixed toxin standard liquid, and calculating the content of the DON, the 3-Ac-DON, the 15-Ac-DON, the ZEN and the T-2 in the wheat sample.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and specifically relates to a detection method for multiple toxins in wheat, especially a detection method for DON, 3-Ac-DON, 15-Ac-DON, ZEN and T-2 toxins in wheat. Background technique [0002] Wheat head blight is an important plant disease in my country's wheat producing areas. It not only causes serious yield loss, but also produces many mycotoxins in grains due to the pathogen Fusarium graminearum, which seriously affects the health of humans and animals. Such toxins mainly include deoxynivalenol (DON), 3Ac-DON, 15Ac-DON, T-2 and zearalenone (ZEN). The research on the control of scab in my country has gone through decades. Although important achievements have been made in pesticide control and disease-resistant breeding, the ideal control effect has not been achieved. Especially in the Huanghuai River Basin, the main wheat producing area in my country, wheat varieties are absolutely Mos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 徐剑宏祭芳史建荣殷宪超
Owner JIANGSU ACAD OF AGRI SCI
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