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Method for determining 16 mycotoxins in tea leaves by ultra-high performance liquid chromatography-tandem mass spectrometry

A high-performance liquid chromatography and ultra-high-performance liquid chromatography technology, applied in the field of analytical chemistry, can solve the problems of unstable detection methods of various mycotoxins, inability to detect multiple mycotoxins at the same time, low detection efficiency, etc., and achieve low cost and high extraction efficiency. The effect of shortening the time and improving the recovery rate

Pending Publication Date: 2020-07-03
福建省农业科学院农业质量标准与检测技术研究所
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Problems solved by technology

[0006] At present, the detection methods of multiple mycotoxins in tea at home and abroad are unstable and have low sensitivity. They can only detect one or several toxins at the same time, and the detection efficiency is low. Multiple Mycotoxins in Tea, Different Degrees of Fermented Tea

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  • Method for determining 16 mycotoxins in tea leaves by ultra-high performance liquid chromatography-tandem mass spectrometry
  • Method for determining 16 mycotoxins in tea leaves by ultra-high performance liquid chromatography-tandem mass spectrometry
  • Method for determining 16 mycotoxins in tea leaves by ultra-high performance liquid chromatography-tandem mass spectrometry

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Embodiment 1

[0059] 1 Materials and methods

[0060] 1.1 Instruments and reagents

[0061] UPLC H-Class ultra-high performance liquid chromatography-tandem mass spectrometer (Waters, USA), Waters HSS T3 column (100.0 mm × 2.1 mm, particle size 1.7 μm), TDL-5-A low-speed large-capacity centrifuge (Shanghai An Pavilion Scientific Instruments), KQ-500DE CNC Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.), Type pure water instrument (China Millipore Co., Ltd.), HD-2500 multi-tube vortex mixer (Hangzhou Youning Instrument Co., Ltd.).

[0062] QuEChERS extraction salt bag (4g magnesium sulfate, 1g sodium chloride, 1g sodium citrate, 0.5g disodium citrate salt hydrate, American Waters company), dSPE purification tube (150mg magnesium sulfate, 25mg PSA, 25mg C18, 7mg GCB , American Waters Company), Oasis PRIME HLB small column (3cc, 150mg, American Waters Company), GHP filter membrane (American Pall Company).

[0063] Methanol, acetonitrile (LC-MS grade, Merck Company, Germany), a...

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Abstract

The invention provides a method for determining 16 mycotoxins in tea leaves by ultra-high performance liquid chromatography-tandem mass spectrometry, and belongs to the technical field of analytical chemistry. The method comprises: extracting a sample by formic acid / acetonitrile (10: 90); adding a QuEChERS salt packet, shaking and centrifuging; treating an extracting solution through an OASIS PRIME HLB small column and a Dspe purification pipe, separating by using a Waters HSS T3 chromatographic column, adopting a multi-reaction ion monitoring (MRM) mode of simultaneously scanning positive ions and negative ions, matching a tea substrate with a standard solution, and quantifying by using an isotope internal standard method. The method is stable, accurate, sensitive and rapid, and can meetthe requirements of analyzing various toxin residues in various tea leaves.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for determining 16 kinds of mycotoxins in tea by ultra-high performance liquid chromatography-tandem mass spectrometry. Background technique [0002] Mycotoxins are secondary toxic metabolites produced by certain fungi under certain environmental conditions and harmful to humans and animals. More than 400 kinds of mycotoxins have been found so far, mainly including aflatoxin, ochratoxin A, patulin, deoxynivalenol and T-2 toxin in trichothecene toxins, zearalenone, voltaic acid Horse toxin, versicolor, citrinin and so on. These toxins are acutely toxic to humans and animals through oral or skin inhalation, and can also act on the liver, kidney, nervous system, endocrine system and immune system, and have teratogenic, carcinogenic, mutagenic, toxic kidney damage, hepatotoxicity, Chronic toxicity including immunosuppression and reproductive disturbances. Chi...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/7266G01N30/8637G01N30/8679G01N2030/045G01N2030/062
Inventor 刘文静傅建炜黄彪韦航
Owner 福建省农业科学院农业质量标准与检测技术研究所
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