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Improved QuEChERS-LC-MS/MS method for rapidly determining 13 bactericides in gastrodia elata

A technology for rapid determination and bactericide, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of enhancing penetration, reducing matrix interference, and accelerating dissolution and extraction

Pending Publication Date: 2021-12-03
CHANGCHUN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has many applications in fruits, vegetables, fungi, tobacco, etc., but there are few reports on the application in Gastrodia elata

Method used

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  • Improved QuEChERS-LC-MS/MS method for rapidly determining 13 bactericides in gastrodia elata
  • Improved QuEChERS-LC-MS/MS method for rapidly determining 13 bactericides in gastrodia elata
  • Improved QuEChERS-LC-MS/MS method for rapidly determining 13 bactericides in gastrodia elata

Examples

Experimental program
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Effect test

Embodiment 1

[0039] 1.1 Standard solution preparation

[0040] Accurately measure 10 μL (concentration of 1000 μg·mL -1 ) of the above 13 kinds of fungicide standard solutions, dilute to 10mL with methanol, and prepare a concentration of 1μg·mL -1 The mixed standard stock solution is stored in a -20°C refrigerator and diluted to a concentration of 0.001, 0.01, 0.1, 1, 10, 25, 50, 100 (μg·mL -1 ) standard solution, to be tested.

[0041] 1.2 Preparation of the test solution

[0042] Extraction: Accurately weigh 1g of Gastrodia elata sample powder (through No. 3 sieve), put it in a 50mL polystyrene centrifuge tube, add 5mL of ultrapure water, vortex and mix well, let it stand for 30 minutes, add 5mL of 0.1% formic acid acetonitrile, and shake Vigorously shake on the shaker (500 times / min) for 2 minutes, add 2g of anhydrous magnesium sulfate and 0.5g of sodium chloride mixture, shake it up immediately, shake vigorously on the shaker for 2 minutes, and centrifuge at 10000r / min at 4°C for 10...

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Abstract

The invention relates to an improved QuEChERS-LC-MS / MS method for rapidly determining 13 bactericides in gastrodia elata, and belongs to the field of pesticide residue detection. The method is improved on the basis of an original method, and medicinal materials are soaked in water before being extracted, so that the infiltration capacity of an extraction solvent is enhanced, and dissolution and extraction of a target compound are accelerated. Due to the fact that the physicochemical properties of different compounds differ greatly, in order to achieve simultaneous detection and meet the requirement for the recovery rate, the volume ratio of medicinal materials, water and acetonitrile and the concentration of formic acid are optimized, the final optimization result is that 1 g of medicinal materials, 5 mL of water and 5 mL of 0.1% formic acid acetonitrile are adopted, the average recovery rate of all the compounds ranges from 83% to 112% through detection, and detection requirements are met. According to the method, an improved QuEChERS method is used for extracting and purifying a sample, the residues of the 13 bactericides in the gastrodia elata are detected in combination with triple quadrupole tandem mass spectrometry, and the method has the advantages of being rapid, simple, convenient, environmentally friendly, low in cost and the like.

Description

technical field [0001] The invention belongs to the field of pesticide residue detection, and in particular relates to an improved QuEChERS-LC-MS / MS method for rapidly determining 13 fungicides in Gastrodia elata. Background technique [0002] Gastrodia elata as a traditional Chinese medicinal material is the dried tuber of the orchid plant Gastrodiaelata Bl., which has the effects of relieving wind and spasm, calming liver yang, dispelling wind and dredging collaterals. The National Health Commission of the People's Republic of China has included Gastrodia elata in the list of traditional Chinese medicinal materials with the same origin as medicine and food. As a traditional Chinese medicinal material with the same origin as medicine and food, Gastrodia elata is very popular among consumers. [0003] At present, the research on Gastrodia elata mainly focuses on its chemical composition and pharmacological effects, while the determination and analysis of its residual pestici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/04G01N30/14G01N2030/042G01N2030/146
Inventor 陈丽娜彭韵洁刘春明时东方王晶张语迟王奇冉文倩
Owner CHANGCHUN NORMAL UNIVERSITY
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