3 results about "Bacterial isolate" patented technology

The present invention relates to a method for reducing WST-8 to formazan based on NAD(P)H for determining the susceptibility of certain bacterial isolates to infection by certain bacteriophages, thereby allowing direct observation of the results measured as cell death of the target bacteria. The reduction of tetrazolium salts to formazan by NAD(P)H is used to determine the metabolic activity of cells and as an indicator of cell viability; whereas WST-8 is used to qualitatively measure the bacteriophage killing activity of the target bacteria. The present invention thus belongs to the field of medical biology, in particular to in vitro laboratory test methods.

The present disclosure provides a bioprocess for the simultaneous production of Polyhydroxybutyrate (PHB) and Violacein pigment in a single fermentation using a Himalayan bacterial isolate Iodobacter sp. PCH 194, having accession number MTCC 25171. PHB has plastic properties, renewable origin and bio-degradable nature. It can be used for various packaging applications replacing the petrochemicals-based plastics, thus providing greener alternative to environment. Violacein pigment has anti-oxidant, anti-tumoral, anti-bacterial, and photo-protective properties and can be used in cosmetics and pharmaceuticals applications. The present disclosure provides production process of two valuable products i.e. PHB and violacein pigment in a single bioprocess, therefore, is economically attractive, time saving, and commercially feasible process.

Provided herein is the first commensal microbial gene encoding an enzyme that catalyzes the conversion of androstenedione to epitestosterone in the gut bacterium Clostridium scindens (desF). Also provided is an important and unrecognized capability for epitestosterone in androgen receptor-dependent prostate cancer cell proliferation and the potential clinical relevance of this bacterial enzymatic pathway (desF) in prostate cancer. Additionally, it was discovered that bacterial isolates from urine or prostatectomy tissue are capable of androgen production and that urinary tract bacteria can exhibit 17β-hydroxysteroid dehydrogenase activity The gene in urinary isolates encoding 17β-hydroxysteroid dehydrogenase that catalyzes the conversion of androstenedione to testosterone was identified and named desG.