7 results about "Bacterial isolate" patented technology

The present invention relates to a method for reducing WST-8 to formazan based on NAD(P)H for determining the susceptibility of certain bacterial isolates to infection by certain bacteriophages, thereby allowing direct observation of the results measured as cell death of the target bacteria. The reduction of tetrazolium salts to formazan by NAD(P)H is used to determine the metabolic activity of cells and as an indicator of cell viability; whereas WST-8 is used to qualitatively measure the bacteriophage killing activity of the target bacteria. The present invention thus belongs to the field of medical biology, in particular to in vitro laboratory test methods.

The present invention relates to the field of probiotics, especially lactic acid bacterial strains such as newly identified lactic acid bacterial isolates or extracts therefrom for use as active ingredients suitable for compositions for administration. The present invention further relates to use of these active ingredients for treatment, preventive and / or general beneficial or prophylactic effect of subjects suffering with or affected by a condition, disease or syndrome caused by or associated with lipid metabolism such as impaired lipid metabolism. The active ingredients have in particular been shown to have an adipolysis effect.

This disclosure contemplates detecting antimicrobial heteroresistance to guide antibiotic or other antimicrobial therapy. In certain embodiments, this disclosure contemplates avoiding a monotherapy with antibiotics to which an individual bacterial isolate is heteroresistant, because this can lead to treatment failure. Thus, in certain embodiments, one uses a combination of antibiotics to which a bacterium is individually heteroresistant.

The systems, methods, and devices disclosed herein provide antibiotic resistance prediction using a pan-antibiotic resistance prediction (PARP) model. The PARP model includes a machine learning system trained with a training dataset of genetic information associated with multiple bacterial species and / or antibiotic signature information associated with multiple antibiotics. The PARP model is deployed to a cloud-based service for scalability, providing access to the PARP model for clinic, hospital, and / or laboratory devices. For example, a web-based portal of the cloud-based service receives genomic sequences associated with specific bacterial isolates uploaded via a remote device. The PARP model outputs a predictive index of antibiotic resistance for the specific bacterial isolate. The predictive index may include a bar graph (e.g., displayed in a graphical user interface) showing susceptibility / resistance predictions for multiple antibiotics for the specific bacterial isolate.

The present disclosure provides a bioprocess for the simultaneous production of Polyhydroxybutyrate (PHB) and Violacein pigment in a single fermentation using a Himalayan bacterial isolate Iodobacter sp. PCH 194, having accession number MTCC 25171. PHB has plastic properties, renewable origin and bio-degradable nature. It can be used for various packaging applications replacing the petrochemicals-based plastics, thus providing greener alternative to environment. Violacein pigment has anti-oxidant, anti-tumoral, anti-bacterial, and photo-protective properties and can be used in cosmetics and pharmaceuticals applications. The present disclosure provides production process of two valuable products i.e. PHB and violacein pigment in a single bioprocess, therefore, is economically attractive, time saving, and commercially feasible process.

Provided herein is the first commensal microbial gene encoding an enzyme that catalyzes the conversion of androstenedione to epitestosterone in the gut bacterium Clostridium scindens (desF). Also provided is an important and unrecognized capability for epitestosterone in androgen receptor-dependent prostate cancer cell proliferation and the potential clinical relevance of this bacterial enzymatic pathway (desF) in prostate cancer. Additionally, it was discovered that bacterial isolates from urine or prostatectomy tissue are capable of androgen production and that urinary tract bacteria can exhibit 17β-hydroxysteroid dehydrogenase activity The gene in urinary isolates encoding 17β-hydroxysteroid dehydrogenase that catalyzes the conversion of androstenedione to testosterone was identified and named desG.

In some embodiments, the present disclosure provides a computer-implemented system for predicting the inhibitory effect of a bacteriophage cocktail on the growth of bacterial isolates, wherein the system comprises a training module, configured to train machine learning models to identify patterns and data structures from interaction data between different bacteriophage cocktails and bacterial isolates at times t and t+1 to fit prediction parameters; and, a prediction module, configured to predict the interaction between a bacteriophage cocktail and a bacterium at time t+1 from the interaction data at time t using the trained learning models. In some embodiments, the present disclosure further comprises a pre-processing module, configured to pre-process interaction data between a bacteriophage cocktail and a bacterial isolate for normalization using filters and / or transformations, and a post-processing module configured to produce rankings and comparisons between the different bacteriophage cocktails and bacterial isolates analyzed. Another embodiment of the present disclosure provides a computer-implemented method for predicting the inhibitory effect of a bacteriophage cocktail on the growth of bacterial isolates. Another embodiment of the present disclosure provides a use of the computer- implemented system for predicting the inhibitory effect of a bacteriophage cocktail on the growth of bacterial isolates.