375 results about "Ancylobacter sp." patented technology

The present invention relates to a novel strain of Bacillus sp. D747 (deposited as FERM BP-8234) and methods for controlling plant diseases and insect pests, comprising administering cultures of Bacillus sp. D747 (including the viable bacteria) or viable bacteria isolated by culturing, on the plant parts such as roots, stems, leaves, seeds, and the like, or in the culture soil.

The present invention relates to a vaccine for the prevention of lactic acidosis in a vertebrate, said vaccine comprising at least one isolated microorganism, or fragment or fragments thereof, wherein said microorganism is capable of producing lactic acid within the gut of said vertebrate, and wherein said microorganism is selected from the group consisting of: Clostridium-like species, Prevotella-like species, Bacteroides-like species, Enterococcus-like species, Selenomonas species, non-dextran slime producing Streptococcus species and non-slime producing lactic acid bacterial isolates.

The invention discloses bacillus aryabhattai, microbial inoculum thereof, a preparation method and application. The bacillus aryabhattai BC104 is beta-cypermethrin degrading bacteria, and the preservation unit is the China General Microbiological Culture Collection center (CGMCC), the preservation date is 15th, Dec, 2015, and the preservation number is CGMCC No.11664; the bacillus aryabhattai belongs to mycomonera, phylum firmicutes, bacillus class, bacilli bacteria, bacillaceae and bacillus. The microbial inoculum with the bacillus aryabhattai as an active ingredient is the microbial inoculum obtained by preforming activation culture and fermentation culture on the bacillus aryabhattai BC104. The preparation method comprises the steps of activation culture and fermentation culture; the application of the microbial inoculum is to prepare a medicine for degrading beta-cypermethrin. The strain disclosed by the invention has the advantages of simple preparation process of the microbial inoculum, low cost, convenience in use and good application prospect.

The present invention relates to thermostable proteinases from thermophilic Bacillus species and their uses in the preparation of nucleic acid samples. The enzymes of the invention are stable and active at 65-80 ° C., but are readily autolysed or denatured above 90° C.

The invention discloses an air purifying material of microorganism absorption degradation, a preparation method and applications thereof. The air purifying material of microorganism absorption degradation is obtained by embedding biological grains with 2 to 4mm grain diameter of pseudomonades or acinetobacter which are cultured and acclimatized in a crosswise way in calcium alginate gel. The air purifying material of microorganism absorption degradation can be used in the long time purification of formaldehyde and benzene series pollution in rooms with obvious effect. The product has the advantages of high activity of immobilized cells, good stability, effectively reducing the content of chemical pollution of formaldehyde and benzene series pollution in rooms, being beneficial for realizing the recycling, regenerating and reusing of biocatalysts, and seriously avoiding the pollution to room environment by microorganisms. The raw material has low cost, the preparation process is simple, easy and convenient in operation, and product can be applied in different reactors.

The invention discloses a promoter with a nucleotide sequence as follows: 1) a nucleotide sequence shown in sequence 1 in a sequence table; 2) a nucleotide sequence which hybridizes with the nucleotide sequence in 1) under strict conditions and has promoter activity; or 3) a nucleotide sequence which has homology of more than 70% with the nucleotide sequence in 1) and has promoter activity. The promoter provided by the invention can be applied to the biotechnology field with corynebacterium or escherichia bacteria as industrial microbes. Experiments prove a fragment with promoter activity from SEQ ID NO:1 has the activity higher than that of a trc promoter (SEQ ID NO:2).

The invention discloses denitrifying phosphorus removal bacteria bacillus cereus H-hrb01, and belongs to the technical fields of biological engineering and environmental engineering, wherein bacillus cereus H-hrb01 belongs to bacillus genus by identification and is conserved in the general microbiology centre of the China committee for culture collection of microorganisms, the conservation date is March 27th 2012, and the conservation number is CGMCC NO.5939; bacillus cereus H-hrb01 can take various organic matters as carbon sources, has good denitrifying and phosphorus-removing efficiency, and can be used for sewage biological denitrification and dephosphorization process; bacillus cereus H-hrb01 is activated and cultivated, and fed into artificial wastewater to mix and then introduced into an SBR (styrene butadiene rubber) biofortification device, and each effluent index of the reactor is superior to that of a contrast system which is not fed with the bacterial strain. The removal rates of the COD (chemical oxygen demand), ammonia nitrogen and dissoluble phosphorus in the sewage are respectively up to 93.90%, 97.70% and 96.25%.

The invention discloses bacteria capable of synchronously removing organic substances and ammonia nitrogen in water of a slightly polluted water source under low-temperature and aerobic conditions and a screening and domesticating method, which relate to bacteria and a screening and domesticating method and solve the problem that the prior bacteria are unsuitable for treating the slightly polluted water source as well as low temperature treatment. The bacteria SRA10 of the invention are acinetobacter lwoffi which belong to acinetobacter baumannii and are preserved in China General Microbiological Culture Collection Center on January 19th, 2009, with a preservation number of CGMCC No.2889. The screening method comprises the following steps: I, separating and purifying; II, preparing bacterial solution; III, screening and cultivating a bacterial solution; IV, screening and cultivating a bacterial solution with a live strain; V, repeating the step IV for 1 to 2 times, and stepwise domesticating the live strain; and VI, taking a bacterial solution of the domesticated strain, and rescreening the bacterial solution to obtain the bacteria. The bacteria obtained by screening and domesticating can process water of the slightly polluted water source under a condition of a low temperature of 2 to 10 DEG C.

The invention relates to a Bacillus siamensis YJ15 and an application thereof in biological control of blueberry gray mold. Bacillus siamensis strain YJ15 of that invention belong to the genus Bacillus sp., Deposited in the Common Microorganism Center of China Microorganism Culture Collection Management Committee (CGMCC), strain collection No. CGMCC No. 15952. Bacillus siamensis YJ15 is inoculatedin LB liquid culture medium to obtain fermentation seed liquid, and then the fermentation seed liquid is inoculated in fermentation culture liquid to obtain fermentation broth or supernatant of fermentation broth to obtain biological microbial agent. The biological bacterial agent prepared by the invention can control the blueberry gray mold and has good control effect equivalent to carbendazim.

The invention belongs to the technical field of microorganisms, particularly relates to a method for preparing Iturin A and homolugues thereof. The method comprises the following steps of: culturing bacillus strains and genetic improved strains thereof into a first-stage liquid culture medium, wherein the bacillus strains and genetic improved strains thereof are capable of producing the Iturin A and homolugues thereof; then, using as seed liquid and inoculating into a second-stage liquid culture medium; adding a certain proportion of adsorption and fixing cell material and thickening agent into the second-stage liquid culture medium before inoculating the seed liquid; and carrying out fermentation culture and feeding and supplying culture on the second-stage liquid culture medium after inoculating the seed liquid; and finally collecting the Iturin A and homolugues thereof from the fermentation culture liquid after the fermentation is finished. The invention has the advantages of simple process, convenient operation, high yield, easy industrialization, and the like.

The invention discloses a bacillus strain for degrading aflatoxin B1 and screening and application thereof. The class of the strain is named bacillus aryabhattai DT and perserved in the China GeneralMicrobiological Culture Collection Center, with a perservation number of CGMCC 14949. A treatment method comprises: strain screening and separation by using a medium taking coumarin as a unique carbonsource, and 16S rDNA authentication. Meanwhile, the degradation effect of the fermentation culture solution of the strain on aflatoxin B1 is explored. A single colony of bacillus aryabhattai on a solid medium is picked and inoculated in 50mL of growth medium and then vibration is performed for 12-24h on a shaking table at 30-40 DEG C; the fermentation culture solution of the bacillus aryabhattaireacts with 2mu g/mL aflatoxin B1 for 3-4d in a dark place to realize a degradation rate of 82.98%. The screened bacillus aryabhattai realizes an obvious degradation effect on aflatoxin B1 and has thecharacteristics of high efficiency, energy saving, environmental friendliness and the like, and can be applied to the preparation of relevant aflatoxin detoxification agents.

The present invention relates to Salmonella enterica comprising at least one pgl operon of Campylobacter jejuni or a functional derivative thereof and presenting at least one N-glycan of Campylobacter jejuni or N-glycan derivative thereof on its cell surface. In addition, it is directed to medical uses and pharmaceutical compositions thereof as well as methods for treating and/or preventing Campylobacter and optionally Salmonella infections and methods for producing these Salmonella strains.

The invention discloses a method for improving a paper strengthening effect of a bacterial cellulose-based paper strengthening agent. Bacterial cellulose disclosed by the invention is cellulose secreted by bacterial microbes and synthesized under different culture conditions, and has an English name of Bacterial Cellulose (BC for short). The microbes secreting the cellulose comprise acetobacter, agrobacterium, pseudomonas, achrombacter, alcaligenes, aerobacter, azotobacter, rhizabium, sarcina and the like. The paper strengthening agent refers to an assistant which is added in the paper making process of wood fibers, non-wood vegetable fibers or secondary fiber pulp and can be used for strengthening physical properties of paper. According to the method for improving the effect of the bacterial cellulose-based paper strengthening agent, disclosed by the invention, quaternary ammonium salt cationic modification is performed on the bacterial cellulose, so that the effect of strengthening the physical properties of the paper by the bacterial cellulose can be greatly improved or the consumption of the bacterial cellulose is reduced while an equivalent strengthening effect is achieved.

The invention discloses application of elsholtzia volatile oil in anti-bacteria and antibiotic resistant bacteria. The elsholtzia volatile oil is extracted by steam distillation. The elsholtzia volatile oil has certain functions of killing and/or inhibiting growth on bacteria of staphylococcus, escherichia coli, candida, pseudomonas, enterobacter, streptococcus and the like and fungi. The elsholtzia volatile oil has certain functions of killing and/or inhibiting growth on bacteria of drug-resistant staphylococcus, drug-resistant escherichia coli, drug-resistant enterobacter, drug-resistant klebsiella, drug-resistant pseudomonas, drug-resistant acinetobacter, drug-resistant streptococcus, and drug-resistant enterococcus and the like. The elsholtzia volatile oil can be used in industries offood, medicine, health care products, cosmetics and the like and can be used for preparing various forms of products with antibacterial and antibiotic resistant bacteria functions, and belongs to thenew application field of the elsholtzia volatile oil.

The present invention provides a strain of Acinetobacter bacteria NJAU-3 having an effective crop growth promoting function, and applications thereof. According to the present invention, the strain is isolated from the Hebei corn field rhizosphere soil and is classified into the Acinetobacter sp bacteria based on the physiological and biochemical characteristics and the 16S rDNA analysis; the qualitative and quantitative analysis results show that the strain NJAU-3 has strong inorganic phosphorus solubilizing ability; and the seedling raising test results of the biological seedling raising matrix developed by using the strain show that the product developed based on the strain can effectively promote the growth of cucumber plants.

The present invention relates to a composition comprising a biologically pure culture of a fungicidal Paenibacillus sp. strain comprising a variant fusaricidin synthetase lacking a functional adenylation domain in the third module. The present invention also provides a composition comprising a biologically pure culture of a fungicidal Paenibacillus sp. strain or a cell-free extract thereof comprising at least one Paeniserine and at least one Paeniprolixin. Also provided are isolated compounds and methods of treating a plant to control a plant disease with the disclosed compositions and compounds.