477 results about "Ancylobacter sp." patented technology

The invention discloses a multiple primer group for detecting pathogens of reproductive system diseases and a kit thereof. The kit comprises a combination of amplification primers and fluorescent probes of 10 pathogens, the pathogens comprise molluscum contagiosum virus, Snethia bacteria, gardnerella vaginalis, prevotella genus, campylobacter genus, giant globules genus, Atortobacter vaginalis, lactobacillus jensenii, lactobacillus crispatus and inert lactobacillus. The kit contains UDG enzyme and is used for preventing pollution of a PCR amplification product, and the accuracy of a detection result is ensured. The kit provided by the invention contains an internal reference control, can display the occurrence of false negative, and is used for monitoring the collection, transportation, extraction and amplification processes of a to-be-detected sample. According to the kit, the specificity is 100%, the detection sensitivity reaches 10 copies / mL, the specificity is good, and the detection efficiency is high. The kit is used for identifying the reproductive system disease pathogens of a to-be-detected sample, the to-be-detected sample can be vaginal secretion, the operation steps are simple, and the detection period is short.

The invention discloses a high-efficiency lespedeza growth-promoting bacillus Q4 (Bacillus atrophaeus) capable of improving the abundance of dominant bacteria and a preparation method of the high-efficiency lespedeza growth-promoting bacillus Q4. Belongs to the technical field of microorganisms. According to the bacillus atrophaeus Q4 provided by the invention, the conversion from total-effect nutrients to effective nutrients is improved, the consumption of lespedeza to soil nutrients is enhanced, and the abundance of dominant strain groups is increased, so that the effect of promoting the growth of lespedeza is achieved. After the bacillus atrophaeus Q4 provided by the invention is applied, the soil enzyme activity is remarkably improved, the conversion of available nutrients in soil is remarkably enhanced, and the nutrient consumption capability of lespedeza is enhanced. By applying the bacillus atrophaeus Q4, growth and development of lespedeza are promoted, the content of soil hydrolytic nitrogen and available phosphorus is remarkably increased, conversion to soil nutrients is improved, and the conductivity and organic carbon are increased to the maximum extent and are remarkably increased by 142.77% and 64.26% respectively. In the genus level, after being treated by the bacillus atrophaeus Q4, the bacillus (Bacillus) is obviously increased from 0.84% to 2.47%, and the average relative abundance of the pseudomonas (Fictibacillus) is obviously increased from 0.59% to 1.16%. By inoculating the Q4 inoculant, the structure of a soil colony is greatly improved, the relative abundance of a dominant flora is influenced, and the obvious increase of the relative abundance of Bacillus and Fictibacillus is possibly an important factor for promoting plant growth.

The invention relates to the technical field of biomineralization, and particularly discloses an alkali-resistant urease-producing microbial flora for biomineralization of construction joints and a preparation method of the alkali-resistant urease-producing microbial flora. The microbial flora disclosed by the invention is prepared from the following strains with relative abundance: 4.4 to 5.2 percent of bacillus, 0.39 to 0.46 percent of enterococcus, 4.8 to 5.7 percent of alcaligenes, 0.50 to 0.58 percent of cellulomonas, 22.4 to 24.2 percent of lysobacter, 1.7 to 2.4 percent of pseudomonas, 10.8 to 11.9 percent of Savagea, 15.1 to 16.2 percent of sarcina, 30.8 to 32.2 percent of chloridomonas and 0.41 to 0.48 percent of bacillus. The alkali-resistant microbial compound flora with high urease activity in the limited oxygen and normal environment can effectively overcome the defects that urease microorganisms are low in mineralization efficiency under the anoxic condition, and the strength of an obtained consolidated body is not high.

The invention discloses a bacillus strain H7 and an application thereof. The invention provides bacillus velezensis H7 or a progeny of the bacillus velezensis H7, and the preservation number of the bacillus velezensis H7 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M 2025630. The bacillus velezensis H7 disclosed by the invention has a very good antibacterial effect on various common clinical pathogenic bacteria (including klebsiella pneumoniae, escherichia coli, candida albicans, enterobacter cloacae complex, acinetobacter baumannii and the like), and particularly shows a very high inhibition rate on some drug-resistant clinical pathogenic bacteria; therefore, the compound has important application value in development of drug and non-drug antibacterial products (such as medical antibacterial materials and the like), a new choice can be provided for drug and non-drug prevention and control of clinical pathogenic bacterium infection, and particularly, the compound has a supplementary effect in the aspects of reducing drug-resistant bacterium propagation risk and reducing drug dependence.

The invention provides a high-temperature-resistant cellulose degrading bacterium and application thereof, belongs to bacillus (Bacillus), and is named as Bacillus licheniformis C1. The high-temperature-resistant cellulose degrading bacterium has the advantages that the high-temperature-resistant cellulose degrading bacterium can be used for degrading high-temperature-resistant cellulose; according to the invention, continuous enrichment culture is carried out in a cellulose degradation culture medium under a high-temperature condition, a high-temperature-resistant cellulose degradation bacterium C1 is separated, and the strain C1 is a species of bacillus through preliminary identification of multiphase taxonomy. The filter paper enzyme activity of C1 obtained through screening at the temperature of 50 DEG C reaches 14.014 + / -0.438 U / ml, the endo-cellulase activity reaches 1.664 + / -0.225 U / ml, the exo-cellulase activity reaches 3.909 + / -0.374 U / ml, and the C1 can play a role in lignocellulose treatment and fertilizer preparation.

The invention provides an environment-friendly microbial strain and a water treatment agent preparation process, and relates to the field of water treatment. The environment-friendly microbial strain is prepared from the following raw materials in parts by weight: 20 parts of nitrifying bacteria, 20 parts of denitrifying bacteria, 35 parts of bacillus and 25 parts of EM bacteria, the nitrifying bacteria comprise nitrosomas and nitrobacteria, and the denitrifying bacteria comprise nitrosomas and nitrobacteria. The denitrifying bacteria comprise denitrifying bacilli and sternobacter, the bacillus comprises bacillus subtilis and bacillus licheniformis, and the EM bacteria comprise rhodopseudomonas, lactococcus lactis and saccharomyces cerevisiae. Through flora screening and culture, the culture efficiency is improved, through a fermentation process, fermentation liquor overflow and insufficient dissolved oxygen caused by excessive foam are effectively prevented, the stability of the fermentation process is ensured, through preparation preparation, a good packaging material and a sealing technology, moisture absorption and pollution of a microbial inoculum are prevented, and the quality and safety of a product are ensured.

The present invention addresses the problem of providing a microorganism which has less load on the environment, has excellent capability of controlling various plant diseases, and can be effectively utilized for the control of plant diseases and the like in the form of cells, a culture product of the cells, or a culture supernatant contained in the culture product.　The problem can be solved by using Bacillus sp. 201106_1 strain (NITE BP-03884 strain).

The invention discloses a bacillus megaterium Q7 for promoting the growth of leucaena leucocephala and improving the abundance of a probiotic microbial population. The bacillus megaterium Q7 is a bacillus megaterium for promoting the growth of leucaena leucocephala. Belongs to the technical field of microorganisms. The bacillus megatherium Q7 provided by the invention improves the conversion from total-effect nutrients to effective nutrients, and enhances the consumption of the leucaena leucocephala on soil nutrients, thereby achieving the effect of promoting the growth of the leucaena leucocephala. After the bacillus megatherium Q7 provided by the invention is applied, the enzyme activity of soil is remarkably improved, the conversion of available nutrients in the soil is remarkably enhanced, and the nutrient consumption capacity of leucaena leucocephala is enhanced. The bacillus megaterium Q7 is applied to promote the growth and development of the leucaena leucocephala, so that the contents of soil hydrolytic nitrogen and available phosphorus are remarkably increased, and the conversion to soil nutrients is enhanced. In the genus level, the average relative abundance of bacillus treated by the bacillus atrophaeus Q7 is increased to 9.97% from 1.33%, the bacillus pseudobecomes the leucaena leucocephala soil community dominant bacteria genus, and the relative abundance of Fictibacillus is increased by about 6 times, which indicates that the inoculation of the strain Q7 enables the leucaena leucocephala soil community structure to be changed, and the leucaena leucocephala soil community dominant bacteria genus is changed. And the increase of the relative abundance of dominant bacteria is promoted. Wherein the bacillus and the Fictibacillus belong to obvious increase of relative abundance and are possibly an important factor for promoting plant growth.

Provided are a mixture of three species of Lactobacillus fermentum strain and Lactobacillus plantarum strain and use thereof. A composition according to one aspect including, as an active ingredient, a mixture of Lactobacillus fermentum strains GB102 and GB103 and Lactobacillus plantarum strain GB104; or a lysate or culture solution thereof, or an extract of the culture solution, can be usefully used for the prevention or treatment of muscle-related disorders or obesity by alleviating the decrease in grip strength induced by aging or reducing the change in intestinal microbial environment induced by aging.

The invention relates to a method for producing irisin. The method comprises the following steps: providing an expression vector containing a nucleotide sequence for coding the irisin; inserting the expression vector into at least one first bacterium belonging to the Agrobacterium genus, thereby obtaining a first Agrobacterium comprising the expression vector; treating at least one plant material with the first agrobacterium comprising the expression vector, thereby obtaining a treated plant material; incubating the treated plant material so that it expresses the irisin; and extracting the irisin. The invention also relates to irisin contained in liposomes, their use as a medicament and route of administration, as well as a synthetic gene comprising a sequence encoding irisin and additional elements.

Provided is a composition for controlling uptake of microparticles into a body, the composition containing a lactic acid bacterium. The lactic acid bacterium is preferably any one selected from bacteria belonging to the genus Lactobacillus and bacteria belonging to the genus Streptococcus. For example, the lactic acid bacterium is a bacterium belonging to Lactobacillus delbrueckii, Lactobacillus paracasei, Lactobacillus gasseri, or Streptococcus thermophilus.

The invention discloses a pseudoarthrobacter sp. T64 for promoting soybean growth and wheat yield increase in saline-alkali soil and application of the pseudoarthrobacter sp. T64. The classification name of the pseudoarthrobacter sp. Is pseudoarthrobacter sp., the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on October 20, 2025, and the preservation number of the strain is CGMCC No.36271. The invention further discloses a preparation method of the pseudoarthrobacter sp. The addition of the pseudoarthrobacter T64 can effectively promote soybean growth and wheat yield increase in the saline-alkali soil.

The invention discloses stropharia rugoso-annulata polysaccharide as well as a preparation method and application thereof. The monosaccharide of the stropharia rugoso-annulata polysaccharide disclosed by the invention comprises glucose, galactose, mannose and galacturonic acid; the weight-average molecular weight of the stropharia rugoso-annulata polysaccharide is 1.4712 * 10 < 4 > to 1.623 * 10 < 4 > Da; the main chain of the stropharia rugoso-annulata polysaccharide contains-> 6)-alpha-D-(O-Me)-Galp-(1->, 1, 6-Glc, 1, 3-Man and 1.3-Gal exist in the main chain or a side chain, and terminal residues contain T-Glc and T-Gal. According to the invention, by constructing a DSS induced UC mouse model, the influence of Stropharia rugosoannulata polysaccharide (SRP1) on UC mice is investigated. Results show that the stropharia rugoso-annulata polysaccharide has a certain treatment effect on ulcerative colitis. In-vivo experiments show that the stropharia rugoso-annulata polysaccharide disclosed by the invention can increase abundance of bacteroides and thick-wall funguses and reduce abundance of proteobacteria and deferribacterium funguses; in-vitro experiments show that the stropharia rugoso-annulata polysaccharide can increase the abundance of beneficial bacteria bacteroides, reduce the abundance of harmful bacteria Escherichia-Shigella and Enterococcus, and increase the content of short-chain fatty acids.

The invention provides application of a probiotic composition in preparation of a medicine for preventing and / or treating Alzheimer's disease, and belongs to the technical field of probiotic medical application. The probiotic composition disclosed by the invention is prepared from lactobacillus rhamnosus, bifidobacterium lactis and bifidobacterium bifidum. According to the probiotic composition disclosed by the invention, on the phylum level, the relative abundance of Verrucomicrobiota (verruca microflora phylum) is improved by the probiotic composition, and the relative abundance of the verrucomicrobiota (verruca microflora phylum) is improved by the probiotic composition; in the level of genus, the probiotic composition increases the relative abundance of the beneficial bacteria genus Akkermansia (Ackermania genus) and Bifidobacterium (Bifidobacterium genus), and in the level of genus Akkermania (Ackermania genus) and Bifidobacterium (Bifidobacterium genus); the behavioral cognition level can be remarkably improved, neuron injury is improved, and intestinal barrier injury is improved.

The invention belongs to the technical field of microorganisms, and particularly relates to an arthrobacter strain and application thereof. The invention provides an arthrobacter strain Arthrobacter pokaliiC2, which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 10, 2024, the preservation date is CGMCC No.31933, and the preservation number is CGMCC No.31933. The arthrobacter strain provided by the invention has the advantages that the arthrobacter strain can be used for preparing a bacterial strain; the arthrobacter strain C2 disclosed by the invention has a relatively strong function of producing indoleacetic acid, and the content of indoleacetic acid in plants can be increased, so that the stress resistance of the plants is improved; dissolution of insoluble phosphorus and potassium in soil can be promoted, soil nutrients are improved, plant growth is promoted, and plant quality is improved; meanwhile, the arthrobacter strain C2 has a certain antagonistic effect on bitter gourd fusarium wilt pathogenic bacteria and can be used for preventing and treating bitter gourd fusarium wilt.

The present invention relates to microencapsulated preparations comprising biopesticide, bio-fungicide, and plant growth promoting agents and uses thereof, as well as to methods for controlling pests and for producing said preparations for managing insect pests. In particular, the invention relates to microencapsulated preparations comprising at least one insect-pathogenic (entomopathogenic) fungus selected from Metarhizium spp. and Beauveria bassiana, and at least one pathogenic Bacillus spp. bacterial strain, preparations and particles comprising these, their use on plant material, methods for controlling pests therewith, and processes for their production.

The invention discloses a preparation method of an aminophilic aminobacter capable of efficiently degrading glyphosate and a microbial inoculum of the aminophilic aminobacter, the aminophilic aminobacter is classified and named as aminophilic aminobacter K2, the aminophilic aminobacter is preserved in the Gansu District Center of the China Industrial Microbiological Culture Collection Center, and the preservation number is GSICC30126. The invention further discloses a preparation method of the aminophilic aminobacter capable of efficiently degrading glyphosate and a microbial inoculum of the aminophilic aminobacter capable of efficiently degrading glyphosate. The Aminobacter sp. Gram-negative bacterium provided by the invention can grow and breed by taking glyphosate as a unique carbon source; the glyphosate can be degraded in a complex matrix environment, can continuously and stably play a role in a complex matrix environment, not only shows high glyphosate degradation efficiency, but also has certain inorganic phosphorus degradation capacity. The strain K2 disclosed by the invention has a wide application prospect in repairing an environment polluted by glyphosate.

The present invention discloses a chitinase-producing desert bacillus and its application, and relates to the field of microbial technology. The present invention discovers a new microbial strain, which is identified as belonging to the genus Desert Bacillus and is named Desert Bacillus sp.MYHB_202305ChI. Experimental verification shows that the strain has the ability to tolerate high salt and secrete chitinase.

The present disclosure relates to Bacillus velezensis (B. velezensis) and a use thereof, and belongs to the technical field of microorganisms. The present disclosure solves one of the problems of the Bacillus in the art such as poor broad-spectrum activity for fungi and bacteria, low inhibitory activity, and limited inhibition range. The B. velezensis QMHF-G5 has an excellent inhibitory activity for pathogenic fungi and bacteria, and can inhibit a variety of pathogenic fungi and bacteria, with a prominent broad-spectrum activity. In addition, the B. velezensis has a high inhibitory activity for a variety of pathogenic fungi and bacteria detected, with a high inhibition rate.

The invention discloses bacillus, a fungicide and application of the fungicide in disease control and plant growth promotion, and relates to the technical field of biocontrol bacteria. The preservation number of the bacillus is CGMCC (China General Microbiological Culture Collection Center) No.3265. The bacillus RD22S2 strain of bacillus is separated and screened from rhizosphere soil of eucommia ulmoides, and experiments prove that the bacillus RD22S2 strain can effectively prevent and control gray mold of eucommia ulmoides leaves and has broad-spectrum fungal disease resistance and growth promoting functions. Therefore, the bacillus provided by the invention not only can be used for preventing and treating plant diseases caused by fungi, but also can be used for promoting the growth of various plants and improving the yield and quality of the plants. Good application prospects of preventing and treating plant diseases and promoting plant growth are realized.

The invention relates to an antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID No. 1. The antibacterial peptide has excellent broad-spectrum antibacterial activity, for example, the antibacterial peptide has antibacterial activity on bacteria of the genus Staphylococcus (Staphylococcus), the genus Acinetobacter (Acinetobacter) and the genus Bacillus (Bacillus). Under the background that the global bacterial drug resistance problem is increasingly severe and traditional antibiotics gradually lose efficacy on multi-drug-resistant bacteria, a new treatment means is provided for treating bacteria such as methicillin-resistant staphylococcus aureus, carbapenem-resistant acinetobacter baumannii and bacillus subtilis which bring huge threats to public health. And a new possibility is provided for solving the problem that no medicine can be used clinically.

Bacillus strains useful for preventing and / or controlling Clostridium perfringens infections and necrotic enteritis in monogastric animals, such as swine and poultry, and for improving the overall health, feed conversion rates and body weight gain of such animals, as well as compositions comprising the Bacillus strains and various methods of using the Bacillus strains in animal feed and animal feed additives.

The invention discloses a microbial complex microbial inoculant, a preparation method thereof and application of the microbial complex microbial inoculant in red mud treatment, and relates to the technical field of mine ecological restoration. The invention provides a composite microbial inoculant which comprises a microbial inoculant microorganism A, a microbial inoculant microorganism B and a microbial inoculant microorganism C in a weight ratio of (1-3): (1-4): 1, the microbial inoculum microorganism A is at least one of rhizobium, azotobacter, pseudomonas, bacillus, burkholderia and glomus; the microbial inoculum microorganism A is at least one of rhizobium, azotobacter, pseudomonas, bacillus, burkholderia and glomus; the microbial agent microorganism B is at least one of desulfurization vibrio, streptococcus, acetobacter and acidophilic thiobacillus; the microbial agent microorganism C is at least one of agrobacterium, enterobacter, streptomyces and klebsiella. The microbial agent disclosed by the invention can effectively solve the problems of high salt and alkali content of the red mud and heavy metal toxicity, and also can create conditions suitable for plant growth on the red mud, so that the ecological restoration effect is improved.

The present invention provides FPR2 agonists. Specifically, an outer membrane vesicle derived from at least one type of lactic acid bacteria selected from the group consisting of the genus Lactobacillus, the genus Lactobacillus casei, the genus Lactobacillus plantarum, the genus Lactobacillus conjuncticus, the genus Lactococcus, and the subsp. Thermophilus of Streptococcus salivarius is compounded as an active ingredient of the FPR2 agonist, and a method for preparing the FPR2 agonist containing the outer membrane vesicle derived from the outer membrane vesicle derived from at least one type of lactic acid bacteria derived from the outer membrane vesicle derived from the at least one type of lactic acid bacteria.

One embodiment of the present invention provides a psicose epimerase expression cassette comprising a polynucleotide encoding a psicose epimerase and a promoter operably linked thereto and modulating the expression of the psicose epimerase in a Corynebacterium strain. The promoter consists of a base sequence as shown in a sequence 1, a base sequence as shown in a sequence 9, a base sequence as shown in a sequence 10 or a base sequence as shown in a sequence 11. The psicose epimerase expression cassette disclosed by the embodiment of the invention is almost not started in a normal environment for culturing a recombinant corynebacterium strain, and does not induce growth inhibition of the recombinant corynebacterium strain. Therefore, the recombinant corynebacterium strain transformed by the psicose epimerase expression cassette of one embodiment of the present invention can normally grow and proliferate to the logarithmic phase, and stably and highly express psicose epimerase after the logarithmic phase.

Modified bacterial strain of the Pseudomonas sp. genus, the strain of Pseudomonas stutzeri deposited under Deposit Accession No. RGM3505 and a modified bacterial strain of the Bacillus sp. genus, a strain of Bacillus velezensis deposited under Accession No. RGM3504. The strains may be combined at a Pseudomonas stutzeri to Bacillus velezensis ratio from 1 :10 to 10:1 CFU / mL. The strains were modified to have greater chemotactic attraction towards plant roots and to improve the beneficial features of crops, for example, of legumes.

The invention provides compositions comprising Achromobacter vitis and methods of using such compositions for preventing and / or treating infections of a plant with a Xylella sp, e.g., Xylella fastidiosa.

The present invention addresses the problem of controlling bacteria of the genus Fusobacterium by a method which is considered to have less impact on beneficial bacteria. The present invention provides a composition for controlling bacteria belonging to the genus Fusobacterium in a digestive tract, particularly in the oral cavity or intestinal flora, the composition containing any one substance selected from the group consisting of a flavonoid that releases a compound represented by formula I in the digestive tract, a transglycosylation product thereof, and a compound represented by formula I.

To provide a promoter for proliferation of nitrate-reducing bacteria in the oral cavity, which enables selective proliferation of nitrate-reducing bacteria in the oral cavity.SOLUTION: A promoter for proliferation of nitrate-reducing bacteria in the oral cavity comprises one or more selected from the group consisting of glycyrrhetinic acid, glycyrrhizic acid, and salts thereof. Preferably, the nitrate-reducing bacteria to be proliferated include one or more species selected from the group consisting of bacteria of the genus Neisseria and bacteria of the genus Haemophilus. The promoter further comprises a nitrate ion-supplying compound. The promoter serves as an agent for preventing or improving dental caries or an agent for periodontal disease.SELECTED DRAWING: None

The objective of this invention is to provide: aerobic-tolerant Bifidobacterium spp. capable of being cultured under aerobic conditions, a method for culturing the same and a method for screening it, and a composition comprising aerobic-tolerant Bifidobacterium spp. and a method for manufacturing the same. The present invention that solves this problem is a method for culturing Bifidobacterium bacteria, comprising the step of culturing Bifidobacterium bacteria having any one of the following (1) to (7) under aerobic conditions: (1) a gene consisting of a base sequence shown in sequence number 1 or 2; (2) a gene having more than 90% identity with a base sequence shown in sequence number 1 or 2; (3) a gene consisting of a base sequence in which 1 to several bases are missing, substituted, or added to a base sequence shown in sequence number 1 or 2; (4) a gene consisting of a base sequence of DNA that can hybridize under strict conditions with a DNA consisting of a complementary sequence of a base sequence shown in sequence number 1 or 2; (5) a gene consisting of a degenerate isomer of a base sequence shown in sequence number 1 or 2; (6) a gene encoding a protein consisting of an amino acid sequence shown in sequence number 3 or 4; (7) a gene encoding a protein consisting of an amino acid sequence in which 1 to several amino acids are missing, substituted, or added to an amino acid sequence shown in sequence number 3 or 4.