409 results about "Ancylobacter sp." patented technology

The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. (As one skilled in the art knows, Xenorhabdus toxin proteins tend to be more desirable for controlling lepidopterans while Photorhabdus toxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.

The present invention relates to a novel strain of Bacillus sp. D747 (deposited as FERM BP-8234) and methods for controlling plant diseases and insect pests, comprising administering cultures of Bacillus sp. D747 (including the viable bacteria) or viable bacteria isolated by culturing, on the plant parts such as roots, stems, leaves, seeds, and the like, or in the culture soil.

A cellular constituent is lysed from, produced by and / or isolated from one or more bacteria from the genus Bacteroides, and the cellular constituent, a derivative thereof, and / or one or more bacteria from the genus Bacteroides, or a modified form thereof, is employed in compositions and methods for modulating an inflammatory response. Such methods include methods of treating, delaying the onset of or reducing the symptoms of one or more inflammatory conditions / diseases, including corporal or gastrointestinal inflammation, for example, Irritable Bowel Syndrome, Crohn's Disease, or colitis, and / or associated diseases such diabetes, asthma, multiple sclerosis, cancer, rheumatoid arthritis, gingivitis, atopic diseases, for example, hay fever, food allergies, eczema, rhinitis, dermatitis, conjunctivitis, atopic syndrome and keratosis pelaris, ocular inflammatory disease, strokes, cardiovascular disease, depression, atherosclerosis and hypertension, and comprise administering a composition comprising one or more natural and / or modified bacteria of the genus Bacteroides, and / or a cellular constituent lysed from, produced by, or isolated from one or more natural and / or modified bacteria from the genus Bacteroides, or a derivative thereof.

The invention provides a method for producing L-lysine by fermentation. The method comprises the following steps: modifying a gene for coding an NCBI reference sequence NP_601029.1 and / or NP_599350.1 on a corynebacterium lilium bacterial chromosome to enable the activity and / or expression quantity of NP_601029.1 and / or NP_599350.1 to be reduced; fermenting germs obtained by modified to produce the L-lysine. Furthermore, the invention further provides a method derived from the method and application, and a germ applied to the methods and the application.

The invention relates to a microbial agent for repairing petroleum-polluted saline alkali soil and a preparation method thereof, and belongs to the technical field of resource environment. According to a principle of microbial ecology, microbial community is extracted and screened from soils with high salt and alkali and high petroleum pollution to prepare the microbial agent, wherein obligate petroleum degrading bacteria can be sphingolipid bacteria, comamonas, chitin autophagy bacteria and bacillus. According to culture medium and soil environment experiments, the microbial agent can guarantee that the extracted microorganism can be survived in the soils with the salt content of 5,000mg / kg, and meanwhile, the degradation capability to the polluted soils with the petroleum content of 10-50g / kg can reach 80%. The microbial agent has the advantages of simple process, rich microorganisms, obvious dominant bacteria, good stability and capability of facilitating obligate petroleum degrading bacteria to adapt to a new pollution environment, increasing and improving the micro-ecological environment quality of the soils and avoiding secondary pollution to the environment, and can obviously improve the degradation efficiency of petroleum hydrocarbon in the saline alkali soil.

The invention relates to a bacillus S-12 of high yield ocean microorganism lysozyme which separated from sea mud of The East China Sea.It is conserved at the type cultivating matter protect center of HuHan China, with CCTCC NO:M204089.The ratio that this S-12 and Bacillus bacterial homology is between 98% and 99%.It is a new fungus ilk with a filiation.The bacteriolysin molecular weight produced by this fungus is 39KD,consisted of 354 aminophenolls. It can be widely used in cosmetic, medicament, disinfector and breeding fields.

The invention discloses a microbiological bacterium for processing waste water in oil refining, its preparing process and use. The bacterium comprises Rhodococcus, Microbacterium spp, bacillus, Candida and Brachybacterium by the ratio of 0-10:0-10:0-10:0-10:0-10:0-10. The bacterium can be activated for treating waste water.

The present invention relates to a vaccine for the prevention of lactic acidosis in a vertebrate, said vaccine comprising at least one isolated microorganism, or fragment or fragments thereof, wherein said microorganism is capable of producing lactic acid within the gut of said vertebrate, and wherein said microorganism is selected from the group consisting of: Clostridium-like species, Prevotella-like species, Bacteroides-like species, Enterococcus-like species, Selenomonas species, non-dextran slime producing Streptococcus species and non-slime producing lactic acid bacterial isolates.

The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.

Novel strains Paenibacillus sp. BS-0048, Paenibacillus sp. BS-0074, Paenibacillus polymyxa BS-0105 and Paenibacillus sp. BS-0277 and Fusaricidin A, Fusaricidin B and novel compounds 3 and 4 produced thereby have an activity of inducing resistance to plant diseases. Thus, they can protect plants from infections with fungi, bacteria, viruses and so on and, as a result, effectively control plant diseases.

A plant growth promoting composition includes beneficial microbes and microbial activators. The beneficial microbes may include Bacillus spp., Azotobacter spp., Trichoderma spp., Saccharomyches spp., or combinations of these, the microtrial activators include enzymes, metabolites, carbohydrate yeast, humic material, staich, amino acids and micornutrients. The plant growth promoting composition may also include organic and inorganic fertilizers.

The invention discloses bacillus aryabhattai, microbial inoculum thereof, a preparation method and application. The bacillus aryabhattai BC104 is beta-cypermethrin degrading bacteria, and the preservation unit is the China General Microbiological Culture Collection center (CGMCC), the preservation date is 15th, Dec, 2015, and the preservation number is CGMCC No.11664; the bacillus aryabhattai belongs to mycomonera, phylum firmicutes, bacillus class, bacilli bacteria, bacillaceae and bacillus. The microbial inoculum with the bacillus aryabhattai as an active ingredient is the microbial inoculum obtained by preforming activation culture and fermentation culture on the bacillus aryabhattai BC104. The preparation method comprises the steps of activation culture and fermentation culture; the application of the microbial inoculum is to prepare a medicine for degrading beta-cypermethrin. The strain disclosed by the invention has the advantages of simple preparation process of the microbial inoculum, low cost, convenience in use and good application prospect.

The present invention relates to thermostable proteinases from thermophilic Bacillus species and their uses in the preparation of nucleic acid samples. The enzymes of the invention are stable and active at 65-80 ° C., but are readily autolysed or denatured above 90° C.

The invention discloses application of a Harmine derivative to preparation of antibacterial medicines. The bacteria is selected from Acinetobacter, Bacillus, Campylobacter, Chlamydia, Chlamydia trachomatis, Clostridium, Citrobacter, Escherichia, enterohemorrhagic escherichia coli, enteric bacteria, Enterococcus, Francisella, Haemophilus, helicobacter, Klebsiella Bacillus, Lester monocytogenes, Moraxella, Mycobacterium, Neisseria, proteus, Pseudomonas, Salmonella, shewanella oneidensis, Shigella, Stenotrophomonas, Staphylococcus, Streptococcus and Yersinia.

The invention discloses an air purifying material of microorganism absorption degradation, a preparation method and applications thereof. The air purifying material of microorganism absorption degradation is obtained by embedding biological grains with 2 to 4mm grain diameter of pseudomonades or acinetobacter which are cultured and acclimatized in a crosswise way in calcium alginate gel. The air purifying material of microorganism absorption degradation can be used in the long time purification of formaldehyde and benzene series pollution in rooms with obvious effect. The product has the advantages of high activity of immobilized cells, good stability, effectively reducing the content of chemical pollution of formaldehyde and benzene series pollution in rooms, being beneficial for realizing the recycling, regenerating and reusing of biocatalysts, and seriously avoiding the pollution to room environment by microorganisms. The raw material has low cost, the preparation process is simple, easy and convenient in operation, and product can be applied in different reactors.

The invention discloses a promoter with a nucleotide sequence as follows: 1) a nucleotide sequence shown in sequence 1 in a sequence table; 2) a nucleotide sequence which hybridizes with the nucleotide sequence in 1) under strict conditions and has promoter activity; or 3) a nucleotide sequence which has homology of more than 70% with the nucleotide sequence in 1) and has promoter activity. The promoter provided by the invention can be applied to the biotechnology field with corynebacterium or escherichia bacteria as industrial microbes. Experiments prove a fragment with promoter activity from SEQ ID NO:1 has the activity higher than that of a trc promoter (SEQ ID NO:2).

The invention discloses bacteria capable of synchronously removing organic substances and ammonia nitrogen in water of a slightly polluted water source under low-temperature and aerobic conditions and a screening and domesticating method, which relate to bacteria and a screening and domesticating method and solve the problem that the prior bacteria are unsuitable for treating the slightly polluted water source as well as low temperature treatment. The bacteria SRA10 of the invention are acinetobacter lwoffi which belong to acinetobacter baumannii and are preserved in China General Microbiological Culture Collection Center on January 19th, 2009, with a preservation number of CGMCC No.2889. The screening method comprises the following steps: I, separating and purifying; II, preparing bacterial solution; III, screening and cultivating a bacterial solution; IV, screening and cultivating a bacterial solution with a live strain; V, repeating the step IV for 1 to 2 times, and stepwise domesticating the live strain; and VI, taking a bacterial solution of the domesticated strain, and rescreening the bacterial solution to obtain the bacteria. The bacteria obtained by screening and domesticating can process water of the slightly polluted water source under a condition of a low temperature of 2 to 10 DEG C.

The invention relates to a Bacillus siamensis YJ15 and an application thereof in biological control of blueberry gray mold. Bacillus siamensis strain YJ15 of that invention belong to the genus Bacillus sp., Deposited in the Common Microorganism Center of China Microorganism Culture Collection Management Committee (CGMCC), strain collection No. CGMCC No. 15952. Bacillus siamensis YJ15 is inoculatedin LB liquid culture medium to obtain fermentation seed liquid, and then the fermentation seed liquid is inoculated in fermentation culture liquid to obtain fermentation broth or supernatant of fermentation broth to obtain biological microbial agent. The biological bacterial agent prepared by the invention can control the blueberry gray mold and has good control effect equivalent to carbendazim.

The present invention relates to novel mixtures comprising, as active components, at least one isolated bacterial strain, which is a member of the genus Paenibacillus, or a cell-free extract thereof or at least one metabolite thereof, and at least one other biopesticide. The present invention also relates to compositions comprising at least one of such bacterial strains, whole culture broth or a cell-free extract or a fraction thereof or at least one metabolite thereof, and at least one other biopesticide. The present invention also relates to a method of controlling or suppressing plant pathogens or preventing plant pathogen infections by applying such composition. The present invention also relates to mixtures of fusaricidins which are pesticidal metabolites produced by the abovementioned strains, and other biopesticides.

The invention belongs to the technical field of microorganisms, particularly relates to a method for preparing Iturin A and homolugues thereof. The method comprises the following steps of: culturing bacillus strains and genetic improved strains thereof into a first-stage liquid culture medium, wherein the bacillus strains and genetic improved strains thereof are capable of producing the Iturin A and homolugues thereof; then, using as seed liquid and inoculating into a second-stage liquid culture medium; adding a certain proportion of adsorption and fixing cell material and thickening agent into the second-stage liquid culture medium before inoculating the seed liquid; and carrying out fermentation culture and feeding and supplying culture on the second-stage liquid culture medium after inoculating the seed liquid; and finally collecting the Iturin A and homolugues thereof from the fermentation culture liquid after the fermentation is finished. The invention has the advantages of simple process, convenient operation, high yield, easy industrialization, and the like.

The invention discloses a bacillus strain for degrading aflatoxin B1 and screening and application thereof. The class of the strain is named bacillus aryabhattai DT and perserved in the China GeneralMicrobiological Culture Collection Center, with a perservation number of CGMCC 14949. A treatment method comprises: strain screening and separation by using a medium taking coumarin as a unique carbonsource, and 16S rDNA authentication. Meanwhile, the degradation effect of the fermentation culture solution of the strain on aflatoxin B1 is explored. A single colony of bacillus aryabhattai on a solid medium is picked and inoculated in 50mL of growth medium and then vibration is performed for 12-24h on a shaking table at 30-40 DEG C; the fermentation culture solution of the bacillus aryabhattaireacts with 2mu g / mL aflatoxin B1 for 3-4d in a dark place to realize a degradation rate of 82.98%. The screened bacillus aryabhattai realizes an obvious degradation effect on aflatoxin B1 and has thecharacteristics of high efficiency, energy saving, environmental friendliness and the like, and can be applied to the preparation of relevant aflatoxin detoxification agents.

Flavor composition suitable for imparting a yogurt flavor to a food product and obtained by fermentation, characterised by a flavor so concentrated that the composition should be diluted at least 2 times, preferably at least 10 times, more preferably at least 50 times to equal the flavor of regular yogurt, which can be obtained by a process, characterised by the following stepsa. fermenting a cheese whey containing medium with a bacterium belonging to the genus Propionibacterium,b. pasteurising the fermentation broth,c. fermenting the pasteurised broth with a yeast preferably one which is unable to ferment lactose,d. pasteurising the fermentation broth,e. optionally adding diacetyl.

The present invention relates to Salmonella enterica comprising at least one pgl operon of Campylobacter jejuni or a functional derivative thereof and presenting at least one N-glycan of Campylobacter jejuni or N-glycan derivative thereof on its cell surface. In addition, it is directed to medical uses and pharmaceutical compositions thereof as well as methods for treating and / or preventing Campylobacter and optionally Salmonella infections and methods for producing these Salmonella strains.

The invention discloses a method for improving a paper strengthening effect of a bacterial cellulose-based paper strengthening agent. Bacterial cellulose disclosed by the invention is cellulose secreted by bacterial microbes and synthesized under different culture conditions, and has an English name of Bacterial Cellulose (BC for short). The microbes secreting the cellulose comprise acetobacter, agrobacterium, pseudomonas, achrombacter, alcaligenes, aerobacter, azotobacter, rhizabium, sarcina and the like. The paper strengthening agent refers to an assistant which is added in the paper making process of wood fibers, non-wood vegetable fibers or secondary fiber pulp and can be used for strengthening physical properties of paper. According to the method for improving the effect of the bacterial cellulose-based paper strengthening agent, disclosed by the invention, quaternary ammonium salt cationic modification is performed on the bacterial cellulose, so that the effect of strengthening the physical properties of the paper by the bacterial cellulose can be greatly improved or the consumption of the bacterial cellulose is reduced while an equivalent strengthening effect is achieved.

The invention discloses a preparation method and application of high-efficiency aerobic denitrification phosphorus-accumulating organism (DPAO) immobilized globules. The method comprises the followingsteps: 1) employing high-efficiency aerobic DPAO, named as Acinetobacter sp., belonging to acinetobacter, and having the preservation number of CGMCC No.17456; 2) selecting aerobic DPAO with preserved slopes, inoculating the aerobic DPAO into a liquid LB culture medium, and carrying out shake cultivation to obtain a bacterial suspension; and 3) embedding the bacterial suspension with a sodium alginate solution to obtain the immobilized spheres. The immobilized spheres are added into domestic sewage according to the adding amount of 20-60 g / l for nitrogen and phosphorus removal treatment, thenitrogen removal rate exceeds 80%, and the phosphorus removal rate exceeds 90%. The object for embedding the spheres is a single strain, and the embedding material is only sodium alginate. According to the process, nitrification liquid backflow is unnecessary, synchronous nitrogen and phosphorus removal can be achieved, the treatment effect is high, the operation process is simple, the productioncost is low, and popularization is easy.