30results about How to "Increase the amount of fermentation" patented technology

The invention provides a method for producing L-lysine by fermentation. The method comprises the following steps: modifying a gene for coding an NCBI reference sequence NP_601029.1 and/or NP_599350.1 on a corynebacterium lilium bacterial chromosome to enable the activity and/or expression quantity of NP_601029.1 and/or NP_599350.1 to be reduced; fermenting germs obtained by modified to produce the L-lysine. Furthermore, the invention further provides a method derived from the method and application, and a germ applied to the methods and the application.

The invention discloses a composite type animal intestine regulating preparation and a preparation method thereof. The composite type animal intestine regulating preparation is mainly compounded from probiotics and prebiotics, wherein the probiotics comprise clostridium butyricum and lactobacillus acidophilus; and the prebiotics comprise lichee polyphenol and glutamine. According to the composite microecological preparation disclosed by the invention, lichee polyphenol and glutamine are utilized to promote clostridium butyricum and lactobacillus acidophilus to proliferate in intestinal tracts, so that the effect of regulating intestinal flora in a two-way mode is brought into play. The composite microecological preparation is relatively good in stability, can regulate intestinal flora of animals, improves disease resistance of the animals, reduces a diarrhea rate and a death rate, can keep a relatively high survival rate, and is suitable for the needs of the feed industry and the livestock breeding industry.

The present invention provides a method for producing L-lysine by fermentation, which comprises modifying a wild-type ppc gene on the corynebacterium glutamicum chromosome; and fermenting the L-lysinewith the modified corynebacterium glutamicum. In addition, the present invention also provides a method and use derived by the method of the invention, as well as corynebacterium glutamicum, polynucleotides and the like which can be used in such methods and use.

The invention provides a method for fermentation production of L-tryptophan. The method comprises: transforming bacteria to reduce their tdcD enzyme expression quantity and/or enzyme activity, even to enable their tdcD enzyme expression quantity and/or enzyme activity to completely disappear; and, applying the transformed bacteria to fermentation production of L-tryptophan. In addition, the invention also provides methods derived from the method and application, as well as polynucleotide, carriers and bacteria usable in the methods and application.

The invention provides a method for fermentation production for L-tryptophan. The method comprises the following steps of: transforming a wild-type regulation component for tdcD genes on a bacterial chromosome so that the expression starting capacity of the regulation component for bacteria obtained by transforming, for the downstream enzyme genes (for example, tdcD genes) of the bacteria is weakened but not disappears; and fermenting by the transformed bacteria to produce L-tryptophan. Additionally, the invention further provides methods and applications derived from the method, and polynucleotides, carriers and bacteria capable of being used in the methods and applications.

The invention provides a thermophilic alkaline pectin lyase gene, an engineering bacterium, thermophilic alkaline pectin lyase and application thereof and belongs to the technical field of biological engineering. The preservation number of the engineering bacterium is CGMCC No.11460, the engineering bacterium is named as Escherichia coli through classification, the depository authority is the General Microbiology Center of China Committee for Culture Collection of Microorganisms, and the preservation date is September 29, 2015. The invention mainly discloses the thermophilic alkaline pectin lyase, the optimum reaction temperature is 70 DEG C, the optimal Ca2+ concentration is 0.8 mM, a substrate is 0.2% (w/v) polygalacturonic acid, and pH is 9.5. The specific activity of the lyase is 900 U/mg under the optimal conditions that the pH is 9.5, the Ca2+ concentration is 0.8 mM and the temperature is 70 DEG C; heat preservation is performed at the temperature of 70 DEG C, and a half-life period is 6 h; after nickel column purification treatment is performed, CbPelC can be up to 50 mg/L (1 L fermented liquid). The heat resistance and yield of the thermophilic alkaline pectin lyase are remarkably improved, the thermophilic alkaline pectin lyase more adapts to the modern industrial production requirements, and an effective method is provided for industrial mass production of the alkaline pectinase.

The present invention provides a method for fermentatively producing L-glutamic acid, which comprises transforming the gene encoding DNA methylase on the chromosome of bacteria of the genus Corynebacterium, reducing the activity and/or expression of DNA methylase; and, using The modified bacteria ferment to produce L-glutamic acid. In addition, the invention also provides a treatment method for glutamic acid fermentation broth.

The invention provides a method for producing L-lysine through fermentation. The method comprises the following steps: modifying the bacteria to weaken but not vanish the expression of aconitase A and/or enzymatic activity, and producing L-lysine by using the modified bacteria through fermentation. In addition, the invention further provides a method and an application derived from the method, and polynucleotide, carriers and bacteria which can be applied to the methods and the application.

The invention provides a fermenting machine used for broussonetia papyrifera fermented feed, which belongs to the technical field of agricultural product processing. The fermenting machine comprises acharge hopper and a fermenting tank, wherein the charge hopper faces towards the fermenting tank; the fermenting tank comprises a cover body and a cylindrical inner barrel body; partition plates arearranged in the inner barrel body for dividing the inner barrel body into at least eight fermenting rooms which are distributed in a radial direction; discharge holes are separately formed in bottomsof the fermenting rooms; each discharge hole is connected with a discharge tube with a valve; an inner cover is arranged on the top of the inner barrel body; an inner-layer charge hole is formed in aposition, which corresponds to each fermenting room, of the inner cover; the cover body sleeves the outer part of the inner barrel body and is rotatably connected with the inner barrel body; and at least one outer-layer charge hole which is matched with the inner-layer charge hole is formed in the cover body, and the charge hopper is directly connected with the outer-layer charge hole.