30results about How to "Increase the amount of fermentation" patented technology

The invention provides a method for producing L-lysine by fermentation. The method comprises the following steps: modifying a gene for coding an NCBI reference sequence NP_601029.1 and / or NP_599350.1 on a corynebacterium lilium bacterial chromosome to enable the activity and / or expression quantity of NP_601029.1 and / or NP_599350.1 to be reduced; fermenting germs obtained by modified to produce the L-lysine. Furthermore, the invention further provides a method derived from the method and application, and a germ applied to the methods and the application.

The invention discloses a composite type animal intestine regulating preparation and a preparation method thereof. The composite type animal intestine regulating preparation is mainly compounded from probiotics and prebiotics, wherein the probiotics comprise clostridium butyricum and lactobacillus acidophilus; and the prebiotics comprise lichee polyphenol and glutamine. According to the composite microecological preparation disclosed by the invention, lichee polyphenol and glutamine are utilized to promote clostridium butyricum and lactobacillus acidophilus to proliferate in intestinal tracts, so that the effect of regulating intestinal flora in a two-way mode is brought into play. The composite microecological preparation is relatively good in stability, can regulate intestinal flora of animals, improves disease resistance of the animals, reduces a diarrhea rate and a death rate, can keep a relatively high survival rate, and is suitable for the needs of the feed industry and the livestock breeding industry.

The present invention provides a method for producing L-lysine by fermentation, which comprises modifying a wild-type ppc gene on the corynebacterium glutamicum chromosome; and fermenting the L-lysinewith the modified corynebacterium glutamicum. In addition, the present invention also provides a method and use derived by the method of the invention, as well as corynebacterium glutamicum, polynucleotides and the like which can be used in such methods and use.

The invention provides a method for fermentation production of L-tryptophan. The method comprises: transforming bacteria to reduce their tdcD enzyme expression quantity and / or enzyme activity, even to enable their tdcD enzyme expression quantity and / or enzyme activity to completely disappear; and, applying the transformed bacteria to fermentation production of L-tryptophan. In addition, the invention also provides methods derived from the method and application, as well as polynucleotide, carriers and bacteria usable in the methods and application.

The invention provides a method for fermentation production for L-tryptophan. The method comprises the following steps of: transforming a wild-type regulation component for tdcD genes on a bacterial chromosome so that the expression starting capacity of the regulation component for bacteria obtained by transforming, for the downstream enzyme genes (for example, tdcD genes) of the bacteria is weakened but not disappears; and fermenting by the transformed bacteria to produce L-tryptophan. Additionally, the invention further provides methods and applications derived from the method, and polynucleotides, carriers and bacteria capable of being used in the methods and applications.

The invention provides an improved L-lysine fermentation preparation method. The method comprises the step of modifying a gene encoding DNA methylase on a chromosome of a corynebacterium bacterium, and reducing the activity and / or expression of the DNA methylase; and, fermenting the bacterium obtained by the modification to produce lysine. In addition, the present invention also provides a methodand an application derived from the method, as well as bacteria and the like that can be used in the method and the application.

The invention provides a method for fermenting to produce L-lysine. The method includes replacing the promoter of one or more genes of the chromosome of the coryneform bacterium with the EP5 promoter, and modifying L-lysine produced by the bacterial fermentation. In addition, the invention also provides methods and applications derived from the method, as well as bacteria and promoters that can be used in these methods and applications.

The invention provides a method for producing L-lysine by fermentation. The method comprises the following steps: modifying a gene for coding an NCBI reference sequence NP_601029.1 and / or NP_599350.1 on a corynebacterium lilium bacterial chromosome to enable the activity and / or expression quantity of NP_601029.1 and / or NP_599350.1 to be reduced; fermenting germs obtained by modified to produce the L-lysine. Furthermore, the invention further provides a method derived from the method and application, and a germ applied to the methods and the application.

The invention relates to the field of food waste deodorization equipment, in particular to a deodorization device for composting and fermentation of food waste, including a main body mechanism, a composting mechanism, a feeding mechanism, a material spreading mechanism and a shaking mechanism; The board runs through the side of the box body, slide the slider on the side wall of the board to the inside of the fixing seat on the side wall of the chain, the stepper motor is powered on to realize that the board for the food waste is lowered to a vacant position, and the next fixing seat is connected with the chain. Corresponding to the feed port, it is easy to install the next placing board. According to this method, several placing boards are installed to store the food waste for fermentation. After the garbage on the surface of the placing board at the bottom of the box is fermented into fertilizer, the bottom cover of the box can be opened The board realizes the cleaning of the fertilizer at the bottom of the box, so as to solve the problem of stratification of the traditional meal waste during the fermentation of the food waste, it can avoid that the fermented waste cannot be processed.

The invention provides a promoter nucleic acid sequence of sdaA gene, a recombinant strain containing the nucleic acid sequence and an application of the recombinant strain. The promoter is obtained by introducing a point mutation into a wild-type promoter sequence of sdaA gene. The recombinant strain is used for fermenting and producing L-lysine to further improve the yield of L-lysine, has no conflict with modification sites of other existing modified strains producing L-lysine, and realizes a new mode for improving the fermentation yield of L-lysine, and thus popularization and applicationare facilitated.

The invention provides a thermophilic alkaline pectin lyase gene, an engineering bacterium, thermophilic alkaline pectin lyase and application thereof and belongs to the technical field of biological engineering. The preservation number of the engineering bacterium is CGMCC No.11460, the engineering bacterium is named as Escherichia coli through classification, the depository authority is the General Microbiology Center of China Committee for Culture Collection of Microorganisms, and the preservation date is September 29, 2015. The invention mainly discloses the thermophilic alkaline pectin lyase, the optimum reaction temperature is 70 DEG C, the optimal Ca2+ concentration is 0.8 mM, a substrate is 0.2% (w / v) polygalacturonic acid, and pH is 9.5. The specific activity of the lyase is 900 U / mg under the optimal conditions that the pH is 9.5, the Ca2+ concentration is 0.8 mM and the temperature is 70 DEG C; heat preservation is performed at the temperature of 70 DEG C, and a half-life period is 6 h; after nickel column purification treatment is performed, CbPelC can be up to 50 mg / L (1 L fermented liquid). The heat resistance and yield of the thermophilic alkaline pectin lyase are remarkably improved, the thermophilic alkaline pectin lyase more adapts to the modern industrial production requirements, and an effective method is provided for industrial mass production of the alkaline pectinase.

The invention provides a method for producing L-threonine through fermentation. The method comprises the steps of transforming the wild type regulatory element of an acnA gene on bacterial chromosome, so that the expression quantity of the aconitase A is lowered, but not disappear; and producing L-threonine through fermentation of the transformed bacteria. In addition, the invention also provides methods and applications derived from the method for producing L-threonine through fermentation, and bacteria used in the methods and applications.

The invention provides a method for fermentatively producing l-threonine, which comprises transforming bacteria to reduce the expression level and / or enzyme activity of aconitase A but not disappearing; and, fermenting and producing l-threonine with the transformed bacteria acid. In addition, the present invention also provides methods and uses derived from this method, as well as polynucleotides, vectors and bacteria that can be used in these methods and applications.

The invention provides a method for fermentatively producing l-threonine, which comprises transforming the wild-type regulatory element of the acnA gene on the bacterial chromosome so that the expression level of aconitase A is reduced but not eliminated; and, using the transformed bacterium Fermentation produces l-threonine. In addition, the present invention also provides methods and applications derived from this method, as well as bacteria and the like that can be used in these methods and applications.

The present invention provides a method for fermentatively producing L-glutamic acid, which comprises transforming the gene encoding DNA methylase on the chromosome of bacteria of the genus Corynebacterium, reducing the activity and / or expression of DNA methylase; and, using The modified bacteria ferment to produce L-glutamic acid. In addition, the invention also provides a treatment method for glutamic acid fermentation broth.

The invention provides a method for producing L-lysine through fermentation. The method comprises the steps of transforming a promoter region of a wild-type ppc gene on a corynebacterium glutamicum chromosome and using corynebacterium glutamicum obtained through transformation to produce the L-lysine through fermentation. In addition, the invention further provides methods and application derivedfrom the method, as well as corynebacterium glutamicum, polynucleotides and the like which can be applied to the methods and application.

The invention provides a method for fermentatively producing L-tryptophan, which comprises transforming the bacterial chromosome fbp The wild-type regulatory element of the gene, so that the regulatory element of the transformed bacterium has its downstream enzyme gene (such as, fbp The ability to initiate expression of the gene) is weakened but not disappeared; and, L-tryptophan is produced by fermentation with the transformed bacteria. In addition, the present invention also provides methods and uses derived from this method, as well as polynucleotides, vectors and bacteria that can be used in these methods and applications.

The invention provides a thermophilic alkaline pectin lyase gene, an engineering bacterium, thermophilic alkaline pectin lyase and application thereof and belongs to the technical field of biological engineering. The preservation number of the engineering bacterium is CGMCC No.11460, the engineering bacterium is named as Escherichia coli through classification, the depository authority is the General Microbiology Center of China Committee for Culture Collection of Microorganisms, and the preservation date is September 29, 2015. The invention mainly discloses the thermophilic alkaline pectin lyase, the optimum reaction temperature is 70 DEG C, the optimal Ca2+ concentration is 0.8 mM, a substrate is 0.2% (w / v) polygalacturonic acid, and pH is 9.5. The specific activity of the lyase is 900 U / mg under the optimal conditions that the pH is 9.5, the Ca2+ concentration is 0.8 mM and the temperature is 70 DEG C; heat preservation is performed at the temperature of 70 DEG C, and a half-life period is 6 h; after nickel column purification treatment is performed, CbPelC can be up to 50 mg / L (1 L fermented liquid). The heat resistance and yield of the thermophilic alkaline pectin lyase are remarkably improved, the thermophilic alkaline pectin lyase more adapts to the modern industrial production requirements, and an effective method is provided for industrial mass production of the alkaline pectinase.

The invention provides a method for producing L-lysine through fermentation. The method comprises the following steps: modifying the bacteria to weaken but not vanish the expression of aconitase A and / or enzymatic activity, and producing L-lysine by using the modified bacteria through fermentation. In addition, the invention further provides a method and an application derived from the method, and polynucleotide, carriers and bacteria which can be applied to the methods and the application.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a glucosamine fermentation production method by high-density Escherichia coli culture. The glucosamine fermentation production method by high-density Escherichia coli culture has the advantages that only conventional Escherichia coli in the prior art is used as a fermentation strain, seed culture media and fermentation culture media are optimized, and accordingly the bacterium count of the Escherichia coli can be effectively increased; the content of glucosamine in fermentation broth can be effectively increased without genetic engineering modification on the strain.

The invention provides a method for fermentation production of L-tryptophan. The method comprises: transforming bacteria to reduce their tdcD enzyme expression quantity and / or enzyme activity, even to enable their tdcD enzyme expression quantity and / or enzyme activity to completely disappear; and, applying the transformed bacteria to fermentation production of L-tryptophan. In addition, the invention also provides methods derived from the method and application, as well as polynucleotide, carriers and bacteria usable in the methods and application.

The invention provides a fermenting machine used for broussonetia papyrifera fermented feed, which belongs to the technical field of agricultural product processing. The fermenting machine comprises acharge hopper and a fermenting tank, wherein the charge hopper faces towards the fermenting tank; the fermenting tank comprises a cover body and a cylindrical inner barrel body; partition plates arearranged in the inner barrel body for dividing the inner barrel body into at least eight fermenting rooms which are distributed in a radial direction; discharge holes are separately formed in bottomsof the fermenting rooms; each discharge hole is connected with a discharge tube with a valve; an inner cover is arranged on the top of the inner barrel body; an inner-layer charge hole is formed in aposition, which corresponds to each fermenting room, of the inner cover; the cover body sleeves the outer part of the inner barrel body and is rotatably connected with the inner barrel body; and at least one outer-layer charge hole which is matched with the inner-layer charge hole is formed in the cover body, and the charge hopper is directly connected with the outer-layer charge hole.

The invention discloses a preparation method of Paecilomyces lilacinus fungus agent, which comprises the following steps: (1) mixing diatomaceous earth, corn flour and water at a mass ratio of 3:3:4 to obtain a solid medium; (2) fermenting: super Add every 200ml of PDB seed bacteria solution to 0.5kg solid medium in the clean bench. The spore concentration in the bacteria solution is 1010-1011cfu / ml. After stirring evenly, put it into a container with a thickness of 2.4-2.6cm, seal the container and ventilate it with oxygen. Sterilize, cultivate at a temperature of 25-28°C and a humidity of 25%-35%, and ferment for 7d-10d to obtain a solid fermented product; (3) make it into granules. At the same time, a fermentation device for Paecilomyces lilacinus agent is also disclosed, in which small holes are set on the side wall of the shallow dish, the small holes are blocked by sterile cotton balls or silica gel plugs, and the plastic film is hooped on the mouth of the shallow dish with rubber bands Edges sealed. The preparation method of the invention has simple process and low cost, is suitable for popularization and application, has simple device and is convenient to use.