A preparation method and device of Paecilomyces lilacinus fungicide

Abstract

The invention discloses a preparation method of Paecilomyces lilacinus fungus agent, which comprises the following steps: (1) mixing diatomaceous earth, corn flour and water at a mass ratio of 3:3:4 to obtain a solid medium; (2) fermenting: super Add every 200ml of PDB seed bacteria solution to 0.5kg solid medium in the clean bench. The spore concentration in the bacteria solution is 1010-1011cfu / ml. After stirring evenly, put it into a container with a thickness of 2.4-2.6cm, seal the container and ventilate it with oxygen. Sterilize, cultivate at a temperature of 25-28°C and a humidity of 25%-35%, and ferment for 7d-10d to obtain a solid fermented product; (3) make it into granules. At the same time, a fermentation device for Paecilomyces lilacinus agent is also disclosed, in which small holes are set on the side wall of the shallow dish, the small holes are blocked by sterile cotton balls or silica gel plugs, and the plastic film is hooped on the mouth of the shallow dish with rubber bands Edges sealed. The preparation method of the invention has simple process and low cost, is suitable for popularization and application, has simple device and is convenient to use.

Description

technical field [0001] The invention relates to the field of disease prevention and control of crops, and more specifically relates to a preparation method of a Paecilomyces lilacinus fungus agent. The preparation process of the invention is simple, low in cost, suitable for popularization and application, and also relates to a Paecilomyces lilacinus fungus The preparation device of the agent is simple and convenient to use. Background technique [0002] Paecilomyces lilacinus ( Paecilomyces lilacinus ) as an important biocontrol fungus, both entomopathogens and nematode pathogens. Paecilomyces lilacinus has a wide control spectrum. In addition to controlling root-knot nematodes and cyst nematodes, Paecilomyces lilacinus can control common soil-borne diseases such as cotton fusarium wilt (Wang Mingzu et al., 1996), rice blight (Xiao Yannong et al., 1996), etc. Higher control effect has better application prospect. At the same time, because Paecilomyces lilacinus fungicide...

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Problems solved by technology

[0003] At present, the production process and equipment of Paecilomyces lilacinus agent use open solid fermentation. The disadvantage is that it is difficult to control the water content and air humidity of the material during the fermentation process, and it is easy to be polluted by miscellaneous bacteria, which leads to fermentation failure. Therefore, it is necessary to develop a short fermentation time. , a preparation method with stable fermentation process and low production cost and a device suitable for the preparation method

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