A method for cultivating Escherichia coli to ferment and produce glucosamine

A technology of glucosamine and Escherichia coli, which is applied in the field of microbial fermentation, can solve the problems of low fermentation density and low ammonia sugar production, and achieve the effects of reducing stimulation, increasing fermentation density, and improving fermentation activity

Inactive Publication Date: 2020-11-03
成都本则生科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] For this reason, the technical problem to be solved by the present invention is to provide a method for cultivating Escherichia coli to ferment and produce glucosamine, so as to solve the problem in the prior art that the fermentation density of bacterial strains is low in the process of producing ammonia sugar by microbial fermentation, which leads to low ammonia sugar production.

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  • A method for cultivating Escherichia coli to ferment and produce glucosamine
  • A method for cultivating Escherichia coli to ferment and produce glucosamine

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Embodiment 15

[0026] Embodiment 1 5L fermentation tank fermentation

[0027] The method for cultivating Escherichia coli to ferment and produce glucosamine described in this embodiment comprises the following steps:

[0028] (1) Inoculate one ring of the Escherichia coli that has been activated and cultivated through the LB plate medium into the seed medium for seed activation, control the culture temperature to 35°C, control the shaker speed to 200rpm, and cultivate for 10 hours to obtain seed liquid;

[0029] The seed culture medium includes: peptone 10g / L, yeast extract 25g / L, glycerin 2g / L, glycine 8g / L, biotin 0.1g / L, adjust pH to 7.0-7.2;

[0030] (2) Inoculate the activated seed liquid into a 5L fermenter with 2.5L fermentation medium according to the inoculum size of 10% to carry out fermentation culture. The control culture temperature is 35°C, the stirring speed is 300rpm, and the ventilation rate is 1.2vvm , culture time is 40h, obtains the fermented liquid containing glucosami...

Embodiment 25

[0033] Embodiment 2 5L fermentation tank fermentation

[0034] The method for cultivating Escherichia coli to ferment and produce glucosamine described in this embodiment comprises the following steps:

[0035] (1) Inoculate one ring of the Escherichia coli that has been activated and cultivated through the LB plate medium into the seed medium for seed activation, control the culture temperature to 37°C, control the shaking table speed to 220rpm, and cultivate for 10h to obtain seed liquid;

[0036] The seed culture medium includes: peptone 20g / L, yeast extract 15g / L, glycerin 4g / L, glycine 3g / L, biotin 0.3g / L, pH 7.0-7.2;

[0037] (2) Inoculate the activated seed liquid into a 5L fermenter with 2.5L fermentation medium according to the inoculation amount of 10% and carry out fermentation culture. The control culture temperature is 37°C, the stirring speed is 250rpm, and the ventilation volume is 1.8vvm , culture time is 40h, obtains the fermented liquid containing glucosami...

Embodiment 35

[0040] Example 3 Fermentation in a 5L fermenter

[0041] The method for cultivating Escherichia coli to ferment and produce glucosamine described in this embodiment comprises the following steps:

[0042] (1) Inoculate one ring of the Escherichia coli that has been activated and cultured through the LB plate medium into the seed medium for seed activation, control the culture temperature to 35° C., control the shaking table speed to 220 rpm, and cultivate for 10 hours to obtain seed liquid;

[0043] The seed culture medium includes: peptone 15g / L, yeast extract 20g / L, glycerin 3g / L, glycine 5g / L, biotin 0.2g / L, adjust pH 7.0-7.2;

[0044] (2) Inoculate the activated seed liquid into a 5L fermenter with 2.5L fermentation medium according to the inoculum size of 10% and carry out fermentation culture. The control culture temperature is 32°C, the stirring speed is 280rpm, and the air flow is 1.5vvm , the culture time is 42h, and the fermentation liquid containing glucosamine is...

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Abstract

The invention belongs to the technical field of microbial fermentation, and particularly relates to a glucosamine fermentation production method by high-density Escherichia coli culture. The glucosamine fermentation production method by high-density Escherichia coli culture has the advantages that only conventional Escherichia coli in the prior art is used as a fermentation strain, seed culture media and fermentation culture media are optimized, and accordingly the bacterium count of the Escherichia coli can be effectively increased; the content of glucosamine in fermentation broth can be effectively increased without genetic engineering modification on the strain.

Description

technical field [0001] The technical field of microbial fermentation of the present invention particularly relates to a method for cultivating Escherichia coli to ferment and produce glucosamine. Background technique [0002] Glucosamine (GlcN), also known as ammonia sugar, glucosamine or glucosamine, is a compound in which one hydroxyl group of glucose is replaced by an amino group. It is an important functional monosaccharide and the first amino group whose structure has been confirmed. Monosaccharide. Glucosamine is easily soluble in water and hydrophilic solvents, and its derivative N-acetylglucosamine (GlcNAc) can be obtained by acid hydrolysis. GlcN exists in almost all organisms, including bacteria, yeast, filamentous fungi, plants and animals, and is the main component of glycoproteins and proteoglycans, as well as chitosan and chitin. [0003] Glucosamine is widely used in the fields of medicine, food and health care. Because glucosamine can specifically act on a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/26C12P19/02C12R1/19
CPCC12N1/20C12P19/02C12P19/26
Inventor 吴红光
Owner 成都本则生科技有限公司
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