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A technology of pectin lyase and genetically engineered bacteria, which is applied in the field of bioengineering, can solve the problems of few reports on thermophilic pectinase, and achieve the effect of increasing the fermentation capacity
Inactive Publication Date: 2016-03-30
JILIN UNIV
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[0005] At present, pectin lyases are mainly alkaline enzymes, and most of them are normal temperature enzymes, and there are
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Embodiment 1
[0035] Example 1: Construction of thermophilic engineering bacteria and expression of enzymes
[0036] (1) Primer design and acquisition of thermophilic alkaline pectin lyase gene PelC by PCR method
[0037] Using the genome of Fusobacterium thermofusiformis CaldicellulosiruptorbesciiDSM6725 as a template, design primers, amplify PelC gene by PCR method, carry out agarose gel electrophoresis to the amplified product, recover and purify 1300bp, sequencing shows that the size of this fragment is 1305bp, and its sequence is as SEQ ID NO .1 shown.
[0038]The sequences of the upstream primers are as follows:
[0062] Embodiment 2: Characteristic of recombinant thermophilic alkaline pectin lyase
[0063] The enzyme activity assay method of thermophilic alkaline pectin lyase described in embodiment 1 is specifically as follows:
[0064] Substrate preparation: Weigh 0.2g of polygalacturonic acid and place it in a 100mL beaker, add about 80mL of 50mM Gly and stir to dissolve it, adjust the pH to 9.5 with 1mol / L NaOH, and dilute the solution to 100mL with a volumetric flask , dubbed 0.2% (w / v) of the substrate.
[0065] Control group: add 100μL, 0.8mM, CaCl to 800μL substrate 2 , preheat at 70°C for 1 min, add 100 μL, 20 mM, pH 8.0 Tris-HCl, and measure the absorbance at 235 nm within 2 min;
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Abstract
The invention provides a thermophilic alkaline pectin lyase gene, an engineering bacterium, thermophilic alkaline pectin lyase and application thereof and belongs to the technical field of biological engineering. The preservation number of the engineering bacterium is CGMCC No.11460, the engineering bacterium is named as Escherichia coli through classification, the depository authority is the General Microbiology Center of China Committee for Culture Collection of Microorganisms, and the preservation date is September 29, 2015. The invention mainly discloses the thermophilic alkaline pectin lyase, the optimum reaction temperature is 70 DEG C, the optimal Ca2+ concentration is 0.8 mM, a substrate is 0.2% (w/v) polygalacturonic acid, and pH is 9.5. The specific activity of the lyase is 900 U/mg under the optimal conditions that the pH is 9.5, the Ca2+ concentration is 0.8 mM and the temperature is 70 DEG C; heat preservation is performed at the temperature of 70 DEG C, and a half-life period is 6 h; after nickel column purification treatment is performed, CbPelC can be up to 50 mg/L (1 L fermented liquid). The heat resistance and yield of the thermophilic alkaline pectin lyase are remarkably improved, the thermophilic alkaline pectin lyase more adapts to the modern industrial production requirements, and an effective method is provided for industrial mass production of the alkaline pectinase.
Description
technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a thermophilic alkaline pectin lyase gene, engineering bacteria, thermophilic alkaline pectin lyase and the application of the enzyme in degrading pectin substances. Background technique [0002] Pectin substances are mainly composed of polygalacturonic acid (GalA), and the side chains often contain trehalose, rhamnose, arabinose, etc., which are widely present in primary plant cell walls. [0003] Pectinase is a general term for a series of enzymes that decompose pectin substances, and is widely distributed in higher plants and microorganisms. During the process of plant maturation, pectinase makes the plant cell wall softer, and also plays an important role in the balance of plant tissue corruption and regeneration. Pectinases can be divided into two categories according to their mode of action, namely esterases and depolymerases, and depolymerases are furth...
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