23 results about "Lysine fermentation" patented technology

The invention provides a method for increasing the fermentation yield of L-lysine, and belongs to the field of L-amino acid fermentation. The method for improving the fermentation yield of L-lysine comprises the following steps: preparing a shake-flask culture solution, preparing a seed solution, and carrying out fermentation culture. According to the method for improving the fermentation yield of the L-lysine, the tolerance of thalli to high osmotic pressure can be effectively improved, the acid production performance in the later period of fermentation is improved, and the single-tank yield and the sugar-acid conversion rate of the L-lysine are improved; meanwhile, the adverse effect of the osmotic pressure regulator on the fermentation stability of the L-lysine is further reduced, and the problems of inhibition of thallus metabolism, prolonging of the fermentation production period and reduction of the fermentation efficiency due to excessive addition of the osmotic pressure regulator are effectively avoided.

The present invention provides a method for increasing the fermentation yield of L-lysine, and belongs to the field of L-amino acid fermentation. The method for increasing the fermentation yield of L-lysine is composed of the following steps: preparing a shake flask culture solution, preparing a seed solution, and fermenting and culturing. The method for increasing the fermentation yield of L-lysine of the present invention can effectively improve the tolerance of the bacterial body to high osmotic pressure, improve the acid production performance in the late fermentation period, and improve the single-tank yield and sugar-acid conversion rate of L-lysine; at the same time, further reduce the adverse effects of osmotic pressure regulators on the fermentation stability of L-lysine, and effectively avoid the problem of excessive addition of osmotic pressure regulators inhibiting bacterial metabolism, extending the fermentation production cycle, and reducing fermentation efficiency.

The invention discloses a process for preparing a liquid fertilizer from a lysine fermentation waste liquid, and relates to the technical field of fertilizer preparation, and the process has the advantages that a specific metal organic framework (MOFs) derived porous carbon material is adopted as an adsorption material to realize efficient purification of lysine in the lysine fermentation waste liquid, the porous carbon material obtained after ZIF-8 is subjected to carbonization and other treatment has a high specific surface area and a rich pore structure, a large number of sites are provided for adsorption of lysine, amino is introduced through ammonia gas plasma treatment and can form hydrogen bonds or electrostatic interaction with carboxyl of lysine and the like, carboxyl is introduced through oxygen plasma treatment, and the adsorption efficiency of lysine is improved. By means of the precise design, lysine is effectively separated from other impurities, a high-purity lysine resource is obtained, the content and quality of effective components in subsequent liquid fertilizer can be improved, and meanwhile the comprehensive utilization efficiency and economic benefits of the resource are greatly improved.

The invention discloses a method for preparing a fertilizer from lysine fermentation waste liquid based on ultrafiltration pretreatment, and relates to the technical field of fertilizer preparation, and the method has the advantages that lignin is modified by a sodium hydroxide solution, phenolic hydroxyl groups react with sodium hydroxide under an alkaline condition to be converted into a carboxylic acid sodium salt structure, and the fertilizer is prepared. Secondly, the mesoporous alumina has a uniform mesoporous channel structure, the contact area with the lignin is increased due to the high specific surface area of the mesoporous alumina, lignin molecules are arranged in order due to the confinement effect of channels, the stability and the activity of the lignin molecules are improved, loading of the lignin is facilitated, and finally, the loading capacity of the lignin is improved. When the composite material is used for treating the lysine fermentation waste liquid, lysine molecules in the waste liquid can be efficiently adsorbed, so that the specific composite material can be used for effectively treating the lysine fermentation waste liquid, the resource utilization rate of the fermentation waste liquid is greatly improved, and the raw material supply for preparing the liquid fertilizer is ensured.

The invention relates to a method for efficiently synthesizing lysine by corynebacterium glutamicum based on multi-level metabolism reconstruction, and belongs to the technical field of metabolic engineering. The method is not limited to single gene or single path transformation, but carries out system integrated regulation and control from multiple levels of lysine main synthesis pathway, central carbon metabolism redistribution, by-product synthesis inhibition and lysine transport and tolerance mechanism, and constructs a ''flux-energy-tolerance'' synergistically matched integral metabolism network; the method is a fundamental technical framework for realizing 240g / L high yield. On the premise that genetic stability of engineering bacteria and controllability of the fermentation process are guaranteed, stable and efficient synthesis of lysine under the high-density fermentation condition is achieved, the final fermentation concentration of lysine stably reaches 240 g / L or above, meanwhile, high carbon source conversion efficiency and low by-product generation level are both considered, and the method is suitable for industrial production. Therefore, the technical bottleneck that the existing lysine biological manufacturing technology is limited to the platform stage of yield of about 200 g / L for a long time is broken through.

The invention discloses a fermentation-membrane separation coupling production method of high-purity lysine, and belongs to the technical field of amino acids, the method comprises the following steps: fermentation: inoculating a corynebacterium glutamicum seed culture solution in a lysine fermentation culture medium, carrying out fermentation culture at 32-34 DEG C for 28-30 hours, and when the fermentation culture is carried out for 17-19 hours, carrying out membrane separation on the fermentation culture medium to obtain the high-purity lysine. N-(4-aminobutyl) acetamide is added; performing membrane separation: performing cross-flow filtration on the fermentation liquor by adopting a surface modified membrane, controlling the inlet pressure to be 0.4-0.5 MPa, the outlet pressure to be 0.2-0.3 MPa and the temperature to be 30-35 DEG C, and obtaining a lysine crude product after the membrane separation is finished; post-treatment: carrying out ion exchange and crystallization on the lysine crude product to obtain a high-purity lysine product; the release of viscous pollutants is reduced from a fermentation source, the pollution resistance of the membrane is improved, the flux recovery rate of the membrane is improved, and efficient coupling of fermentation and separation is realized.

The invention belongs to the technical field of biological agriculture, and particularly discloses L-lysine as well as a production method and application thereof. The method comprises the following steps: S1, carrying out aerobic fermentation on actinobacillus succinogenes, and centrifuging and filtering after fermentation to obtain sterile fermentation liquor; s2, corynebacterium glutamicum is inoculated into a fermentation culture medium for fermentation culture, after the corynebacterium glutamicum is cultured to an acid production period, microalgae active substances and the fermentation liquor obtained in the S1 are added, fermentation culture continues to be conducted for 36-48 h, and L-lysine fermentation liquor is obtained; s3, performing centrifugation, ultrafiltration and concentration on the L-lysine fermentation liquor obtained in the S2, and purifying the L-lysine through ion exchange resin to obtain the L-lysine. The invention discloses L-lysine and a production method and application thereof, the method can significantly improve the yield and purity of L-lysine, can be efficiently and stably applied to preparation of feed additives, and meets the requirements of the feed industry for high quality and safety of amino acid additives.

Disclosed is the use of a PNcgl1969(C200T) promoter and a biomaterial related thereto in the construction of a high-yield L-lysine engineered strain. The promoter is a DNA molecule obtained by means of mutating cytosine at position 200 in the PNcgl1969 promoter to thymine. By means of performing L-lysine fermentation experiments on the constructed engineered strains L-PNcgl1969 and Y-PNcgl1969 containing the PNcgl1969(C200T) promoter, it is found that a point mutation of the PNcgl1969 promoter region in Corynebacterium glutamicum is conducive to improving the yield of L-lysine. The present invention is of great significance for increasing the yield of L-lysine produced by means of bacterial fermentation.

The invention relates to the technical field of microorganisms, and particularly discloses a phosphoenolpyruvate carboxykinase mutant and application thereof in lysine production. The research finds that the 377th amino acid of phosphoenolpyruvate carboxykinase is mutated from proline to serine, so that the yield of lysine produced by zymophyte can be increased, and therefore, the phosphoenolpyruvate carboxykinase mutant is provided, which takes an amino acid sequence as shown in SEQ ID NO.7 or 9 as a reference sequence; the phosphoenolpyruvate carboxykinase mutant contains a 377th amino acid mutation which is mutated from proline to serine. According to the phosphoenolpyruvate carboxykinase mutant provided by the invention, the yield of lysine fermentation can be increased, and a new effective method is provided for lysine production.

The invention discloses a production process of L-lysine hydrochloride based on mother liquor backflow and constant flow distribution, and belongs to the technical field of biological fermentation. The process comprises the following steps: inoculating an L-lysine producing strain seed solution into a fermentation culture medium, carrying out aerobic culture, heating, and adjusting the pH value to obtain an L-lysine fermentation solution; filtering the L-lysine fermentation liquid through a ceramic membrane to obtain a clear liquid, and carrying out ion exchange to obtain an L-lysine purified clear liquid; sequentially neutralizing the L-lysine purified clear liquid with hydrochloric acid, crystallizing, concentrating and centrifuging to obtain mother liquor; finally, dividing the mother liquor into mother liquor 1 and mother liquor 2, and refluxing the mother liquor 1 to the ion exchange process to obtain the mother liquor 1 after ion exchange; and mixing the mother liquor 2 and the mother liquor 1 after ion exchange to obtain a mixed mother liquor, refluxing the mixed mother liquor to a crystallization process, and carrying out heat preservation crystallization after constant-current distribution to obtain the L-lysine hydrochloride. The crystallization stability and the production efficiency of the L-lysine hydrochloride are effectively improved through mother liquor backflow and constant flow distribution.

The invention relates to the technical field of biological fermentation, and discloses a clean production process for improving the fermentation quality and efficiency of L-lysine, which comprises the steps of strain screening, seed culture, fermentation culture, fermentation liquor preparation and product refining. Vitamin compounds are added into the seed culture medium, nutrition is supplemented, seed culture time is shortened, seed vitality is improved, and a good foundation is laid for fermentation. The whole production process is efficient and clean, the production efficiency is high, the energy consumption is reduced, the production process is simple and convenient, the stability of the production process is ensured, and the product quality is improved.

The present application provides a method for producing amino acid and nucleoside by microbial reinforced fermentation, which is achieved by analyzing lysine fermentation process, screening excellent fermentation strains, and adding the preferred E. coli (or adding the preferred C. glutamicum) in a timely and quantitative manner in the fermentation process based on C. glutamicum (or in the fermentation process based on E. coli), so as to obviously improve the yield and conversion rate of lysine. The present application can effectively solve the problems of unstable product quality and difficult effective control of production process in traditional lysine fermentation, and has important significance for improving fermentation efficiency and conversion rate.

The present invention relates to the technical field of microorganisms, and provides a phosphoenolpyruvate carboxykinase mutant and an application thereof in the production of lysine. Studies have found that mutating an amino acid at position 377 of phosphoenolpyruvate carboxykinase from proline to serine increases yield of lysine produced by a fermentation strain. Therefore, provided is a phosphoenolpyruvate carboxykinase mutant, wherein an amino acid sequence shown in SEQ ID NO. 7 or 9 is used as a reference sequence, and the phosphoenolpyruvate carboxykinase mutant comprises a mutation in which the amino acid at position 377 is mutated from proline to serine. The provided phosphoenolpyruvate carboxykinase mutant increases the fermentation yield of lysine and provides an effective new method for lysine production.

This invention discloses a fermentation method for L-lysine, belonging to the field of L-lysine fermentation technology. The fermentation method involves inoculating a secondary *Corynebacterium glutamicum* seed culture at an inoculation rate of 9.8-10.2% into a fermenter containing 1L of fermentation medium. The temperature inside the fermenter is maintained at 34-36℃, the pressure at 0.07-0.08 MPa, and the dissolved oxygen at 20-25%. The pH is maintained at 7-7.2 by adding ammonia. When the residual sugar decreases to 2g / L, a glucose aqueous solution is added to control the residual sugar to 5-8g / L. After 8 hours of fermentation, a betaine supplement is added. After 10 hours of fermentation, a culture medium supplement is added. This fermentation method can improve the L-lysine production rate, L-lysine yield, and sugar-acid conversion rate of *Corynebacterium glutamicum*.

The invention belongs to the technical field of food fermentation production, and particularly relates to streptomyces noursei and application thereof in preparation of epsilon-polylysine. The Streptomyces noorsei QPL-9 is preserved in the China General Microbiological Culture Collection Center on November 27, 2024, the preservation number is CGMCC No.32818, the Streptomyces noorsei is classified and named as Streptomyces noorsei, and the preservation address is Beijing in China. According to the streptomyces noursei, mannitol is used as a carbon source, lysine fermentation waste liquid is used as a nitrogen source for fermentation to prepare the epsilon-polylysine, and the yield of the epsilon-polylysine exceeds 80 g / L.

The invention discloses a lysine fermentation wastewater and waste gas treatment method.The lysine fermentation wastewater and waste gas treatment method comprises the following steps of 1, material adding, 2, drying through a drying tower, 3, secondary drying and 4, gas deodorization. The method is environmentally friendly, efficient, simple in process, stable in heat supply and high in heat efficiency.

The invention relates to the technical field of bioengineering, in particular to a modified corynebacterium microorganism and application thereof in lysine fermentation production. The acetyl coenzyme A synthetase and / or the inactivated NCg11988 gene or the homologous gene thereof are / is heterologously expressed in the corynebacterium, so that synthesis and accumulation of lysine are remarkably promoted, the lysine production capacity of the corynebacterium is effectively improved, and the lysine yield and / or conversion rate of the corynebacterium are / is remarkably improved. The transformation can be used for construction of lysine production strains, the lysine production capacity of the strains is effectively improved, and the method has important significance for improving the production efficiency of lysine and reducing the production cost.

Disclosed is the use of a PNcgl1855 (A244C) promoter and a biomaterial related thereto in preparation of L-lysine. The promoter is a DNA molecule obtained by mutating adenine at position 244 of a PNcgl1855 promoter into cytosine. L-lysine fermentation experiments performed on constructed engineered strains, i.e. L-PNcgl1855 and Y-PNcgl1855, containing the PNcgl1855 (A244C) promoter show that introducing a point mutation into the promoter region of the Ncgl1855 gene in Corynebacterium glutamicum contributes to an increase in the yield of L-lysine. The present invention is of great significance for increasing the yield of L-lysine in bacterial fermentation.

The application discloses a mutant NCgl2165 promoter and application thereof in L-lysine production, and belongs to the field of bioengineering and fermentation engineering.The mutant NCgl2165 promoter is subjected to base substitution at the positions from 477 to 488 in the-10 region relative to the original promoter.The application further discloses an expression cassette, a vector, a host cell containing the promoter and a recombinant corynebacterium glutamicum driven by the promoter pyc The application discloses a mutant NCgl2165 promoter and application thereof in L-lysine production, and belongs to the field of bioengineering and fermentation engineering.The mutant NCgl2165 promoter is subjected to base substitution at the positions from 477 to 488 in the-10 region relative to the original promoter.The application further discloses an expression cassette, a vector, a host cell containing the promoter and a recombinant corynebacterium glutamicum driven by the promoter pyc Gene expression can improve the L-lysine fermentation yield of the corynebacterium glutamicum.In a 5L fermenter, the L-lysine yield of the mutant promoter recombinant strain reaches 157.0 g / L, which is about 22.8% higher than that of the original strain.

A continuous crystallization system suitable for lysine fermentation liquor comprises a storage tank, a first delivery pump, a first-effect falling film evaporator, a second delivery pump, a second-effect falling film evaporator, a third delivery pump, a forced circulation evaporation crystallizer, a crystal separator, a centrifugal machine and a crystal storage tank, and an air inlet of the first-effect falling film evaporator is connected with a steam delivery pipeline through a pipeline. According to the continuous crystallization system suitable for the lysine fermentation liquor, after the lysine fermentation liquor is pre-concentrated by adopting the first-effect falling film evaporator and the second-effect falling film evaporator, the lysine fermentation liquor with higher concentration is finally concentrated and crystallized by the forced circulation evaporation crystallizer, so that excessive scale deposition and excessive energy consumption can be effectively avoided; the crystallization efficiency is high, and the product granularity is uniform; the centrifugal machine is adopted to separate liquid in crystals, the product purity is improved, the separated liquid is conveyed to the forced circulation evaporation crystallizer again to be treated, and the product yield of the lysine fermentation liquor is improved.

The invention discloses a lysine fermentation production pressure control device, which comprises a fermentation tank, the volume of the fermentation tank can be enlarged and reduced by changing the volume of the fermentation tank or the size of a cavity of a container communicated with the fermentation tank; the fermentation tank is provided with a communicated pressure connecting pipe, and the other end of the connecting pipe is provided with a contraction chamber; a spring is arranged in the contraction cavity; and a control push rod is arranged at the other end of the contraction chamber. The defects in the prior art are effectively overcome.

The invention relates to the technical field of lysine evaporative crystallization, in particular to a lysine evaporative crystallization device which comprises a stirring crystallization device, centrifugal separation equipment is arranged on one side of the stirring crystallization device, the centrifugal separation equipment is used for centrifugal separation of lysine crystals, and cold and heat exchange equipment is arranged on one side of the stirring crystallization device. One side of the stirring crystallization device is fixedly connected with an evaporation concentration device, the evaporation concentration device is connected with cold and heat exchange equipment, one side of the stirring crystallization device is rotatably connected with a transmission mechanism, one end of the transmission mechanism extends into the stirring crystallization device and is meshed with the stirring crystallization device, and the stirring crystallization device is rotatably connected with an intermittent device. The intermittent device is matched with the transmission mechanism, a feeding device is arranged at the other end of the evaporation and concentration device, one end of the intermittent device extends into the feeding device and is fixedly connected with the feeding device, and the feeding device is used for conveying, filtering and removing impurities of the lysine fermentation liquor.

The present application relates to the field of microbial technology, and specifically discloses a recombinant microorganism and application of reducing expression amount of aromatic amino acid transporter in fermentation production. The present application finds that inactivation of aromatic amino acid transporter aroP can improve the production efficiency of lysine. Further, a recombinant microorganism is provided, wherein the expression amount of aromatic amino acid transporter aroP is reduced compared with a starting strain; the starting strain is corynebacterium. The present application provides a new and effective way for lysine fermentation production.