Method for preparing lysine through fermenting

A lysine and lysine fermentation technology, which is applied in the field of fermentation to prepare lysine, can solve the problems of reducing extraction costs, shortening extraction process routes, and large consumption of raw and auxiliary materials, so as to reduce extraction costs, shorten extraction process routes, The effect of reducing the consumption of raw materials

Inactive Publication Date: 2015-06-24
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome defects such as the long process route of the lysine fermentation liquid extraction process in the prior art, the consumption of raw and auxiliary materials, and the generation of a large amount of difficult-to-handle waste water and the high...

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  • Method for preparing lysine through fermenting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] This example is used to illustrate the method for preparing lysine by fermentation provided by the present invention.

[0058] (1) Machining 100 parts by weight of harvested corn to pulverize corn grains so that the passing rate of corn flour through a 30-mesh sieve is 80%.

[0059] (2) Add water to the pulverized product to 12Bé°, add 20 enzyme activity units of amylase (α-amylase, Novozymes) relative to the dry weight of each gram of the pulverized product, at 90°C, Enzymolysis was carried out for 100 minutes under the condition of pH 5.5 to obtain an enzymolysis product. Among them, the enzymatic hydrolysis product is filtered through a hydraulic plate and frame filter press to separate the enzymatic hydrolysis liquid (solid content is 20% by weight); after that, 115 enzyme activity units of glucoamylase (α-1,4 - Glucose hydrolase, Novozymes), enzymatically hydrolyze for 420 minutes under the conditions of 60°C and pH 4.5 to obtain the saccharification liquid of sta...

Embodiment 2

[0065] This example is used to illustrate the method for preparing lysine by fermentation provided by the present invention.

[0066] The preparation method of starchy raw material saccharification liquid, the formula of seed tank medium, and the method of culturing mature seed liquid in seed tank are the same as in Example 1.

[0067] Use the saccharification clear liquid of the starchy material obtained to prepare the fermentation medium, with respect to every liter of fermentation medium, the consumption of ammonium sulfate is 15 grams, the consumption of magnesium sulfate is 0.15 grams, other components and their consumptions are all the same as those in Example 1. same. The culture medium was heated to 121°C for 30 minutes, then cooled to 31°C and kept constant, and the pH was adjusted to 6.9 with ammonia water. Determination of SO in the prepared fermentation medium (ie initial medium) 4 2- The content is 11.03 g / L.

[0068] The medium volume in the fermenter was 40%...

Embodiment 3

[0071] This example is used to illustrate the method for preparing lysine by fermentation provided by the present invention.

[0072] The preparation method of starchy raw material saccharification liquid, the formula of seed tank medium, and the method of culturing mature seed liquid in seed tank are the same as in Example 1.

[0073] Use the starchy raw material saccharification clear liquid that makes to prepare fermentation medium, with respect to every liter of fermentation medium, the consumption of ammonium sulfate is 21 grams, the consumption of magnesium sulfate is 0.42 grams, other components and their consumptions are all the same as that of Example 1. same. The culture medium was heated to 121°C for 30 minutes, then cooled to 31°C and kept constant, and the pH was adjusted to 6.9 with ammonia water. Determination of SO in the prepared fermentation medium (ie initial medium) 4 2- The content is 15.61 g / L.

[0074] The medium volume in the fermenter was 60% of th...

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Abstract

The invention discloses a method for preparing lysine through fermenting. The method is characterized by comprising the steps: inoculating a lysine fermentation strain to a lysine fermentation culture medium under the conditions of producing lysine, and carrying out fermentation culture under the conditions of feeding a carbon source and feeding ammonium chloride, wherein the content of SO4<2-> in an initial culture medium is 10-16g/l. According to the method disclosed by the invention, the lysine fermentation liquor, which is obtained through collecting after the fermentation ends, can be subjected to concentrated crystallization directly after thalli are removed, so as to obtain a lysine finished product, and working procedures, such as ion exchange and deamination, are not required to be carried out, so that an extracting process route is shortened, the consumption of raw and auxiliary materials is lowered, no wastewater is generated, the extracting cost is reduced, and the production capacity of lysine is not affected.

Description

technical field [0001] The invention relates to a method for preparing lysine by fermentation. Background technique [0002] As the first limiting amino acid in animals, lysine is not only an indispensable component for protein synthesis, but also participates in energy metabolism in the body, can promote growth and development, enhance immune function, and improve the function of central nervous tissue. [0003] Since pure lysine is difficult to crystallize in the air and is easy to deliquesce, lysine products on the market generally exist in the form of lysine hydrochloride. [0004] Lysine is mainly produced by fermentation, and a certain amount of carbon and nitrogen sources need to be supplemented during the fermentation process to meet the needs of bacterial growth and metabolism to produce lysine. Inorganic nitrogen sources are very important for the growth of microorganisms, especially ammonium nitrogen is an available nitrogen source for microorganisms. In the pri...

Claims

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Application Information

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IPC IPC(8): C12P13/08C12R1/15C12R1/13C12R1/19
Inventor 张军华罗虎熊结青吴晓艳
Owner COFCO BIOTECHNOLOGY CO LTD
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