Production method of lysine

A technology for lysine and lysine fermentation, applied in the field of lysine production, can solve the problems of low economic benefit, high cost, limited lysine production capacity, etc. The effect of amino acid content

Active Publication Date: 2012-07-04
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the fermentation proceeds, the acidity of lysine increases gradually, and with the feeding of carbon and nitrogen sources and feeding during the fermentation process, the concentration of nutrients other than carbon and nitrogen in the fermenter gradually decreases, and lysine Premature senescence and autolysis of acid bacteria strains lead to a decline in metabolic capacity, and the rate of fermentation acid production is on a downward trend. Finally, due to the slow rate of fermentation acid production, the tank is put into storage, which limits the production capacity of lysine, resulting in higher costs and lower economic benefits.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] This example is used to illustrate the production method of lysine provided by the present invention.

[0056] (1) Machining 100 parts by weight of harvested corn to pulverize corn grains, so that the passing rate of corn flour through a 30-mesh sieve is 80%.

[0057] (2) Add water to the pulverized product to 12Bé°, add 20 enzyme activity units of amylase (Novozymes, α-amylase) relative to the dry weight of each gram of the pulverized product, at 90°C, Enzymolysis was carried out for 100 minutes under the condition of pH 5.5 to obtain an enzymolysis product. Wherein, the enzymolysis product is filtered by a hydraulic plate and frame filter press, and the enzymolysis clear liquid (solid content is 20% by weight) is separated; 115 enzyme activity units of glucoamylase (α-1, 4 -glucose hydrolase, Novozymes), enzymolysis at 60° C. and pH 4.5 for 420 minutes to obtain saccharified supernatant liquid of starchy raw materials.

[0058] (3) use the starchy raw material sacch...

Embodiment 2

[0063] This example is used to illustrate the production method of lysine provided by the present invention.

[0064]The preparation method of starchy raw material saccharification liquid, the formula of seed tank medium, the method of culturing mature seed liquid in seed tank, and the formula of fermenter medium are all the same as in Example 1.

[0065] The medium volume in the fermentor was 40% of the fermentor volume. The mature seed liquid is inserted into the culture medium of the fermenter for fermentation and cultivation, and the inoculum amount of the seed liquid is 12% by volume based on the inoculated fermentation medium. After the inoculation, the obtained starchy raw material saccharification liquid and ammonium sulfate were fed continuously, and the amount of the obtained starchy raw material saccharified liquid was fed to control the concentration of reducing sugar in the fermentation broth at 5-7 grams per liter. Add the amount of ammonium sulfate to control t...

Embodiment 3

[0068] This example is used to illustrate the production method of lysine provided by the present invention.

[0069] The preparation method of starchy raw material saccharification liquid, the formula of seed tank medium, the method of culturing mature seed liquid in seed tank, and the formula of fermenter medium are all the same as in Example 1.

[0070] The medium volume in the fermentor was 60% of the fermentor volume. The mature seed liquid is inserted into the culture medium of the fermenter for fermentation and cultivation, and the inoculum amount of the seed liquid is 18% by volume based on the inoculated fermentation medium. After the inoculation, the obtained starchy raw material saccharification clear liquid and ammonium sulfate were fed continuously, and the amount of the obtained starchy raw material saccharified clear liquid was fed to control the concentration of reducing sugar in the fermentation broth at 8-10 grams per liter. Add the amount of ammonium sulfat...

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PUM

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Abstract

The invention discloses a production method of lysine. The method comprises the step of inoculating lysine fermentation strains into lysine fermentation media for fermentation culture under the condition of feeding a carbon source and a nitrogen source. The method is characterized by feeding nutrient salts into fermentation liquor after fermentation culture for 15 hours, wherein the nutrient salts comprise potassium chloride, magnesium sulfate and manganese sulfate. The production method has the following advantages: the content of final lysine, the acid supply quantity per tank and the conversion rate can be improved, namely improving the yield of lysine, thus improving the economic benefits.

Description

technical field [0001] The invention relates to a production method of lysine. Background technique [0002] At present, my country's lysine fermentation adopts the method of batch fermentation. The fermentation uses a certain proportion of each tank to put into the fermentation medium and insert a certain lysine fermentation strain. Under the conditions of feeding carbon source and nitrogen source , for fermentation. As the fermentation proceeds, the acidity of lysine increases gradually, and with the feeding of carbon and nitrogen sources and feeding during the fermentation process, the concentration of nutrients other than carbon and nitrogen in the fermenter gradually decreases, and lysine Premature senescence and autolysis of acid bacteria strains lead to a decrease in metabolic capacity, and the rate of fermentation acid production shows a downward trend. Finally, due to the slow rate of fermentation acid production, the tank is put into storage, which limits the produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08C12R1/13C12R1/15C12R1/19
Inventor 周勇熊结青卢宗梅王梅章辉平
Owner COFCO BIOTECHNOLOGY CO LTD
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