Method for fermenting lysine

A technology of lysine fermentation and fermentation method, which is applied in the field of biological fermentation engineering, can solve the problems of long fermentation time and large power consumption, and achieve the effects of high fermentation acid production, reduced cooling water consumption, and convenient lysine metabolism

Inactive Publication Date: 2008-01-16
NINGXIA EPPEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For the secondary species, because the young bacteria have changed the previous preparation method, the young bacteria can get nutrient-rich substances in the medium, and their growth is vigorous, and the metabolic cult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment one: a kind of method of lysine fermentation, this method comprises the following steps: bacterial classification is moved to secondary seed tank after culturing 9 hours from inclined test tube to big seed bottle, seed amount is 0.2%, and culture temperature is: 38-40°C, after 15 hours of cultivation, move to the third-level seed tank with a seed volume of 5%, and after 9 hours of cultivation, move to a 350m3 fermenter for fermentation. After the third-level seeds are inserted, due to the low sugar osmotic pressure, the seeds grow vigorously and produce a large The heat speeds up the fermentation speed. After 3 hours of cultivation, when the residual sugar in the base material drops from 2% to below 1.4%, it starts to add sugar continuously. The sugar added is glucose, with a volume content of 70% and a seed content of 16%. , The flow acceleration rate in the early stage of sugar feeding is slow, about 1.0 tons of sugar is added per hour; , due to the rapid r...

Embodiment 2

[0022] Embodiment two: a kind of method of lysine fermentation, this method comprises the steps: bacterial classification is moved to secondary seed tank after culturing 11 hours from inclined test tube to big seed bottle, seed amount is 0.25%, and culture temperature is: 38-40°C, after 17 hours of cultivation, move to the third-level seed tank, the seed volume is 6%, and after 11 hours of cultivation, move to a 350m3 fermenter for fermentation. After the third-level seeds are inserted, due to the low sugar osmotic pressure, the seeds grow vigorously and produce a large amount The heat speeds up the fermentation speed. After 4 hours of cultivation, when the residual sugar in the bottom material drops from 4% to below 1.4%, the sugar is added continuously. The sugar added is glucose, the volume content is 60%, and the seed amount is 18%. 1. The flow acceleration rate in the early stage of sugar feeding is slow, about 1.0 tons of sugar is added per hour; , due to the rapid repro...

Embodiment 3

[0023] Embodiment three: a kind of method of lysine fermentation, this method comprises the following steps: bacterial classification is moved to secondary seed tank after culturing 10 hours from inclined test tube to large seed bottle, seed amount is 3%, and culture temperature is: 38-40°C, after 16 hours of cultivation, move into the third-level seed tank with a seed volume of 5.2%, and after 10 hours of cultivation, move into a 350m3 fermenter for fermentation. After the third-level seeds are inserted, due to the low sugar osmotic pressure, the seeds grow vigorously and produce a large amount The heat speeds up the fermentation speed. After 5 hours of cultivation, when the residual sugar in the bottom material drops from 6% to below 1.4%, the continuous flow of sugar is started. The added sugar is glucose, the volume content is 65%, and the seed amount is 14%. , The flow acceleration rate in the early stage of sugar feeding is slow, about 1.0 tons of sugar is added per hour,...

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Abstract

The invention relates to a biology ferment technology field, in particular to a lysine fermentation method. The invention is characterized in that: the method includes the following steps: strains fostered 9 to 11 hours from a slant tube to a large seed bottle are transferred to a secondary seed pot with a seed rate of 0.2 to 0.3 percent and a foster temperature of 38 to 40 DEG C, which are transferred into third seed pot after 15 to 17 hours with a seed rate of 5 to 6 percent, and then transferred to ferment pot after fostering 9 to 11 hours to be fermented. After strains are inoculated to ferment pot, sugar and organic nitrogen are fed continuously, with a seed rate of 14 to 18 percent and a ferment L-sorbose of 2 to 8 percent. The invention is unique in technique with short ferment time which can save energy consumption by three steps of large seed rate intermittent feeding of sugar and nitrogen, with lysine produced at a rate more than 16.5 percent , glucose acid invert ratio above 62 percent, a single batch feeding volume 420 cubic meter by a 350 cubic meter ferment pot , and a ferment period 45 hours.

Description

technical field [0001] The invention relates to the technical field of biological fermentation engineering, in particular to a method for lysine fermentation. Background technique [0002] In the process of lysine production, lysine must first be synthesized by microbial strains. To make cells accumulate lysine excessively, it is necessary to artificially change the metabolic regulation of the strains according to the metabolic characteristics of the strains, so that the metabolism follows the human Carry out in the required direction, accumulate lysine and release it from the bacterial cells, and achieve the best effect of sugar conversion. [0003] In order to meet the needs of large-scale industrial production, there must be a sufficient amount of vigorous purebred seeds. After having the purebred seeds, the seeds must be expanded and cultivated step by step. Only by cultivating the seeds well can the fermentation acid production be high, the conversion high, and satisf...

Claims

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Application Information

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IPC IPC(8): C12P13/08
Inventor 马吉银
Owner NINGXIA EPPEN BIOTECH
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