Method for preparing bio-organic fertilizer by utilizing corn steep liquor and lysine fermentation waste liquid

A technology of lysine fermentation and bio-organic fertilizer, which is applied in microorganism-based methods, preparation of organic fertilizers, treatment of bio-organic parts, etc. The effect of income

Inactive Publication Date: 2019-02-05
SHOUGUANG GOLDEN FAR EAST MODIFIED STARCH CO LTD +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology uses both corn steeping liquors (which are used for making alcohol) from brewery processes and lysis residues produced during production. It also involves preparing biofertilizers through hydrolysis with ammonia water instead of traditional methods like chemically treatments that have negative environmental impacts such as pollution concerns associated therewith. Overall this method provides an efficient way to utilizing these sources without causing harmful side-effects while producing high quality products at low cost.

Problems solved by technology

This patented technical solution describes methods used during corn brewing operations to treat residual or spent yeast extract (YE). These solutions involve concentrating YE soluble components into an aqueous phase followed by precipitation/elecrotic lyte reduction techniques such as centrifugations, membrane separation, ion exchange resin columns, etc., resulting in pure yellowish green liver distillers' grains called XLVG. Additionally, these technologies require complicated handling and may produce odors when applied over long periods of storage due to their volatility properties.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Put the lysine fermentation waste liquid in the separation equipment, mix, cool down and generate supersaturation, and precipitate crystals to obtain liquid fertilizer mother liquor; Bacillus subtilis and Bacillus licheniformis are mixed according to the weight ratio of 1:1 to obtain mixed biological bacteria agent;

[0024] 2) Using corn steep liquor and liquid fertilizer mother liquor to prepare a liquid medium, the medium includes the following raw materials according to weight percentage: corn steep liquor 17%, liquid fertilizer mother liquor 12%, calcium chloride 0.58%, glucose 6%, and the balance is water;

[0025] 3) Transfer the culture medium into a fermenter, add 0.001% medium quality mixed biological agent, and cultivate it for 18 hours at 37°C, with a pressure of 0.1Mpa and an air volume of 0.2vvm, to obtain a spore content of 10 billion / g bacterial liquid;

[0026] 4) Mix corn steep liquor, liquid fertilizer mother liquor, and bacterial liquor according...

Embodiment 2

[0028] 1) Put the lysine fermentation waste liquid in the separation equipment, mix, cool down and generate supersaturation, and precipitate crystals to obtain liquid fertilizer mother liquor; Bacillus subtilis and Bacillus licheniformis are mixed according to the weight ratio of 3:4 to obtain mixed biological bacteria agent;

[0029] 2) Using corn steep liquor and liquid fertilizer mother liquor to prepare liquid culture medium, the culture medium includes the following raw materials according to weight percentage: corn steep liquor 18%, liquid fertilizer mother liquor 9%, calcium chloride 0.48%, glucose 4.7%, and the balance is water;

[0030] 3) Transfer the culture medium into a fermenter, add 0.001% of the medium quality mixed biological agent, and cultivate it for 18 hours at 41°C, with a pressure of 0.1Mpa and an air volume of 0.2vvm, to obtain a spore content of 11.5 billion / g bacterial liquid;

[0031] 4) Mix corn steep liquor, liquid fertilizer mother liquor, and ba...

Embodiment 3

[0033] 1) Put the lysine fermentation waste liquid in the separation equipment, mix, cool down and generate supersaturation, and precipitate crystals to obtain liquid fertilizer mother liquor; mix Bacillus subtilis and Bacillus licheniformis according to the weight ratio of 3:2 to obtain mixed biological bacteria agent;

[0034] 2) Using corn steep liquor and liquid fertilizer mother liquor to prepare a liquid medium, the medium includes the following raw materials according to weight percentage: 20% corn steep liquor, 10% liquid fertilizer mother liquor, 0.5% calcium chloride, 5% glucose, and the balance is water;

[0035] 3) Transfer the culture medium into a fermenter, add 0.001% medium quality mixed biological agent, and cultivate it for 18 hours at 40°C, with a pressure of 0.1Mpa and an air volume of 0.2vvm, to obtain a spore content of 12 billion / g bacterial liquid;

[0036] 4) Mix corn steep liquor, liquid fertilizer mother liquor, and bacterial liquor according to the...

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PUM

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Abstract

The invention discloses a method for preparing bio-organic fertilizer by utilizing corn steep liquor and lysine fermentation waste liquid, relates to the field of agricultural production waste treatment, and mainly solves the problem that an existing corn steep liquor and lysine fermentation waste liquid treatment technology is complicated. The method comprises the following steps: (1) putting thelysine fermentation waste liquid in separation equipment, and separating out crystals, thus obtaining a liquid fertilizer mother solution; (2) preparing a liquid culture medium by utilizing the cornsteep liquor and the liquid fertilizer mother solution; (3) inoculating a mixed biological bacterial agent to the liquid culture medium for culturing, thus obtaining a bacteria solution; (4) uniformlymixing the corn steep liquor, the liquid fertilizer mother solution and the bacteria solution, and then carrying out decomposition fermentation, thus obtaining the bio-organic fertilizer. According to the method disclosed by the invention, the corn steep liquor and the lysine fermentation waste liquid can be fully utilized, and waste of resources is avoided; meanwhile, plenty of treatment steps are not required, sewage and exhaust gas cannot be generated, the whole process is environment-friendly, the technological process is simple and environment-friendly, and the corn steep liquor and theliquid fertilizer mother solution can be treated to a large scale.

Description

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Claims

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Application Information

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Owner SHOUGUANG GOLDEN FAR EAST MODIFIED STARCH CO LTD
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