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Recycling method of lysine fermented waste liquid

A technology for lysine fermentation and fermentation waste liquid, which is applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of limited use of wastewater and high cost of medium raw materials, and achieves high utilization rate and crude oil. The effect of high protein content and enhanced enzyme activity

Active Publication Date: 2017-09-15
齐齐哈尔龙江阜丰生物科技有限公司
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AI Technical Summary

Problems solved by technology

The Chinese patented technology "Method for producing Debaria hansenii for feeding by fermenting high-salt amino acid waste water" discloses a method for recycling and utilizing amino acid waste water to ferment and produce bacterial protein. The basic components are, high-salt amino acid sewage: 125g / L-330g / L, carbon source: 30-100g / L, potassium dihydrogen phosphate (KH 2 PO 4 ): 5-15g / L, yeast powder: 0-10g / L, magnesium sulfate (MgSO 4 ): 3-9g / L, calcium carbonate (CaCO 3 ): 0.75-2g / L, ferrous sulfate (FeSO 4 ): 1.5-4g / L, and the pH of the medium is 3.5-7.5. This method effectively treats wastewater and prepares bacterial protein; however, this method also has some defects: the cost of medium raw materials is high, and the amount of wastewater used is limited, etc. defect

Method used

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  • Recycling method of lysine fermented waste liquid

Examples

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Effect test

Embodiment 1

[0030] A method for recycling lysine fermentation waste liquid, comprising the steps of:

[0031] Step 1) Adjust the pH: add a sulfuric acid solution with a concentration of 2M to the fermentation waste liquid, and adjust the pH of the fermentation waste liquid to 6.8;

[0032] Step 2) Prepare strain culture medium: take 1L as an example, take glucose 90g, yeast powder 30g, MgSO 4 12g, K 2 HPO 4 5g, KH 2 PO 4 5g, FeSO 4 1g and CaCO 3 1g, add to the fermentation waste liquid obtained in step 1), adjust the pH to 6.8, and set the volume to 1L;

[0033] Step 3) Preparation of compound bacterial liquid: Bacillus subtilis seed liquid (1×10 8 individual / ml) and Bacillus licheniformis seed solution (1×10 8 per ml) were mixed according to the volume ratio of 3:1, and then transferred to the strain culture solution according to the volume ratio of 6%, and cultivated at 32°C for 12 hours to obtain the composite bacterial solution;

[0034] Step 4) Prepare algae culture sol...

Embodiment 2

[0040] A method for recycling lysine fermentation waste liquid, comprising the steps of:

[0041] Step 1) Adjust the pH: add a sulfuric acid solution with a concentration of 3M to the fermentation waste liquid, and adjust the pH of the fermentation waste liquid to 6.8;

[0042] Step 2) Prepare strain culture medium: take 1L as an example, take glucose 90g, yeast powder 30g, MgSO 4 12g, K 2 HPO 4 5g, KH 2 PO 4 5g, FeSO 4 1g and CaCO 3 1g, add to the fermentation waste liquid obtained in step 1), adjust the pH to 6.8, and set the volume to 1L;

[0043] Step 3) Preparation of compound bacterial liquid: Bacillus subtilis seed liquid (1×10 8 individual / ml) and Bacillus licheniformis seed solution (1×10 8 per ml) were mixed according to the volume ratio of 4:1, and then transferred to the strain culture solution according to the volume ratio of 6%, and cultivated at 32°C for 12 hours to obtain the composite bacterial solution;

[0044] Step 4) Prepare algae culture sol...

Embodiment 3

[0050] The lysine fermentation waste liquid used in the present invention is the raffinate after extracting lysine and bacteria from the fermentation liquid, and the content of each main component is: sugar 15-20g / L, fatty substance 3-5 g / L L. Crude protein 20-30g / L, COD 1500-2000mg / L, ammonia nitrogen 200-300mg / L and SS 150-200mg / L.

[0051] Taking Example 1 as an example, the components in the fermented liquid obtained in the analysis step 7) are: COD is 27 mg / L, ammonia nitrogen is 11 mg / L and SS is 18 mg / L; It can reach more than 100mg / L, and at the same time, the level of pollutants in the liquid is greatly reduced.

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Abstract

The invention belongs to the technical field of environmental protection, and discloses a recycling method of lysine fermented waste liquid. The method comprises the following steps: 1) regulating pH value; 2) preparing a strain culture solution; 3) preparing composite bacterium liquid; 4) preparing an algae culture solution; 5) preparing an enlarged culture algae solution; 6) preparing a fermentation culture solution; and 7) implementing fermenting and filtering. According to the method provided by the invention, effective utilization of the fermented waste liquid and agricultural wastes can be achieved; water quality is purified while mycoprotein is obtained; load of a sewage treatment device is reduced; and obvious ecological and economic benefits are guaranteed.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a recycling method for lysine fermentation waste liquid. Background technique [0002] Lysine is colorless and is one of the essential amino acids for the human body. It can promote human development, enhance immune function, and improve the function of central nervous tissue. Since the content of lysine in grain food is very low, and it is easily destroyed and lacked during processing, it is called the first limiting amino acid. Free lysine is easy to deliquesce, turn yellow and deteriorate, but its hydrochloride is stable and easy to store, so it is generally supplied as its hydrochloride in the market. At present, the total annual production capacity of lysine in the world reaches 1.6 million tons, of which the annual production capacity in my country is 600,000 tons (only the output of feed grade lysine is 450,000 tons), and the market is still...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P13/08C12R1/10C12R1/125C12R1/89
CPCC12P13/08C12P39/00
Inventor 王峰汲广习唐永强沈兆胜聂庆胜
Owner 齐齐哈尔龙江阜丰生物科技有限公司
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