Method for preparing lysine

A lysine and lysine fermentation technology, applied in the field of lysine preparation, can solve the problems of low conversion rate, low acid supply in a single tank, and uncontinuous production, so as to achieve an increase in conversion rate and acid supply in a single tank The effect of increasing the quantity and reducing the production cost

Inactive Publication Date: 2012-04-04
COFCO BIOTECHNOLOGY CO LTD
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a new lysine in order to overcome the low conversion rate of the preparation method of lysine in the prior art and the low acid supply amount of a single tank, and the defects that the production cannot be carried out continuously after the seed tank is infected with bacteria. Preparation

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  • Method for preparing lysine
  • Method for preparing lysine

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preparation example Construction

[0013] The invention provides a method for preparing lysine, which comprises the steps of inserting lysine fermentation strains into lysine fermentation culture liquid, and carrying out fermentation culture under the conditions of feeding carbon source and nitrogen source The lysine fermenting strain contains strains fermented and cultured for 8-30 hours under the lysine fermentation culture condition.

[0014] According to the present invention, the purpose of the present invention can be achieved by inserting the strains containing 8-30 hours of fermentation culture under lysine fermentation culture conditions into the culture liquid for fermentation culture, namely improving conversion rate and single tank acid supply amount, and the production can be carried out continuously, but in order to facilitate the collection of strains and make full use of the strains cultured by fermentation, preferably, the culture solution is loaded in one or more groups of fermentors, each grou...

Embodiment 1

[0059] This example is used to illustrate the preparation method of lysine provided by the present invention.

[0060] (1) Machining 100 parts by weight of harvested corn to pulverize corn grains, so that the passing rate of corn flour through a 30-mesh sieve is 80%.

[0061](2) Add water to the pulverized product to 12Bé°, add 20 enzyme activity units of amylase (Novozymes, α-amylase) relative to the dry weight of each gram of the pulverized product, at 90°C, Enzymolysis was carried out for 100 minutes under the condition of pH 5.5 to obtain an enzymolysis product. Wherein, the enzymolysis product is filtered by a hydraulic plate and frame filter press, and the enzymolysis clear liquid (solid content is 20% by weight) is separated; 115 enzyme activity units of glucoamylase (α-1, 4 -glucose hydrolase, Novozymes), enzymolysis at 60° C. and pH 4.5 for 420 minutes to obtain saccharified supernatant liquid of starchy raw materials.

[0062] (3) use the starchy raw material sacch...

Embodiment 2

[0068] This example is used to illustrate the preparation method of lysine provided by the present invention.

[0069] The preparation method of the starchy raw material saccharification liquid, the formula of the seed tank culture medium, and the formula of the fermenter culture liquid are all the same as in Example 1.

[0070] Insert the activated and proliferated Brevibacterium flavum strains (the original strain FB42 was purchased from Jiangnan University) into the seed tank for cultivation. During the cultivation process, samples were taken for microscopic examination every 120 minutes and the OD value was determined. When normal and the OD value reached 0.8, the culture was stopped.

[0071] The volume of the culture solution in the 1# fermenter is 40% of the volume of the 1# fermenter. The seed solution cultivated in the seed tank was inserted into the culture solution of the 1# fermenter for fermentation and cultivation. Taking the inoculated culture solution as a ben...

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Abstract

The invention provides a method for preparing lysine. The method comprises the following steps of: inoculating lysine fermentation strains into lysine fermentation culture solution, and performing fermentation culture under the condition of a feeding carbon source and a feeding nitrogen source. The method is characterized in that the lysine fermentation strains contain strains which are subjected to the fermentation culture for 8 to 30 hours under the condition of the fermentation culture of the lysine. By the method, the conversion rate and single-tank acid supply amount are improved substantially, the production can be performed continuously, the production capacity is improved, and the production cost is reduced.

Description

technical field [0001] The invention relates to a preparation method of lysine. Background technique [0002] Lysine is one of the eight essential amino acids for human and animal nutrition. It plays an important role in regulating the metabolic balance in the body, improving the absorption of cereal protein in the body, improving human dietary nutrition and animal nutrition, and promoting growth and development. [0003] At present, it is mainly used in medicine, food and feed industry. From the perspective of consumption structure, the consumption of lysine in feed accounts for nearly 90%, while the consumption of food and pharmaceutical intermediates only accounts for 10%. [0004] Lysine is mostly prepared by microbial fermentation, generally by inoculating lysine fermentation strains into fermenters to produce lysine. In industrial production, fermenters generally include one or more parallel fermenters, and the lysine fermentation strains in each fermenter are obtain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08C12R1/13C12R1/15C12R1/19
Inventor 周勇卢宗梅吴晓艳钟华廖四祥
Owner COFCO BIOTECHNOLOGY CO LTD
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