3results about How to "Reduce toxic effects" patented technology

The present application relates to the technical field of fluorescent probe, and discloses a fluorescent probe locked by viscosity and glycosidase, and a preparation method and application thereof.The present application provides a compound, the fluorescence emission intensity of which is enhanced with the enhancement of the solution viscosity; meanwhile, after incubation with beta-galactosidase, the fluorescence intensity of the compound at 640nm is enhanced.Therefore, in the early diagnosis imaging of ovarian cancer, the compound can be used as a fluorescent probe locked by viscosity and glycosidase, and is used for accurate imaging of ovarian cancer.The fluorescent probe of the present application greatly improves the selectivity of the probe by using a double activation mechanism, effectively avoids non-specific activation to normal tissues, and is particularly suitable for targeted imaging of specific pathological environments such as tumors.

The invention provides a manioc waste liquid feed preparation process based on microbial fermentation, and belongs to the technical field of feed. According to the application, the nano calcium phosphate loaded high-valence ferric salt and the metal ion doped hydroxyapatite are introduced to solve the problems of excessive metal ions and anti-nutrient substance residues; the nano calcium phosphate loaded high-valence ferric salt efficiently oxidizes and removes cyanide ions and tannin in the fermentation liquor through ferrate loaded on the surface; oxidation products and part of ferric ions are fixed, so that inhibition on microorganisms is avoided; the metal ion-doped hydroxyapatite effectively captures excessive metal ions through ion exchange and complexation, reduces the free concentration of the metal ions, prevents adverse effects on microbial metabolism, can compete with anions in a fermentation liquid, inhibits the generation of toxic complexes, and reduces the toxicity to a fermentation system; the synergistic effect of removal of anti-nutrient substances and dynamic regulation and control of metal ions is achieved, and the nutritional value and safety of the final feed are improved.

The present application relates to the field of bioengineering, and particularly relates to a mutant of mu-conotoxin and a preparation method and an enzyme composition thereof.The present application provides a mutant of mu-conotoxin, which has an amino acid sequence as shown in SEQ ID NO:1, or an amino acid sequence obtained by substituting, deleting or adding one or more amino acids in the amino acid sequence, and an amino acid sequence which is functionally identical or similar to the amino acid sequence, or an amino acid sequence which is at least 80% identical to the amino acid sequence.The technical route and process provided by the present application greatly improve the production efficiency and quality of mu-conotoxin, are suitable for industrial large-scale production, and have a wide application prospect.