Method for enhancing fermentation yield of lysine

A technology for lysine fermentation and yield, applied in the field of microbial fermentation, can solve the problem of high cost of glucose, and achieve the effects of low price, easy crushing, and increased yield

Inactive Publication Date: 2015-04-08
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a new method for improving lysine fermentation yield in order t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 conventional lysine fermentation production method

[0020] Conventional seed tank medium is: glucose 25g / L, corn steep liquor 3g / L, KH 2 PO 4 ·3H 2 O15g / L, MgSO 4 ·7H 2 O 0.4g / L, urea 4g / L, FeSO 4 ·7H 2 O 0.01g / L, MnSO 4 ·H 2 O 0.01g / L, the culture conditions used for seed tank culture are: inoculum size 1‰, temperature 37°C, ventilation ratio 1:0.5vvm, tank pressure 0.05Mpa, full control of DO30% or more, pH6.90, cycle 12h.

[0021] The seed tank seed liquid of conventional culture is inserted into conventional fermenter culture medium (glucose 140g / L, ammonium sulfate 50g / L, KH 2 PO 4 ·3H 2 O 4.5g / L, MgSO 4 ·7H 2 O 0.5g / L, FeSO 4 ·7H 2 O 0.015g / L, MnSO 4 ·H 2 O 0.015g / L, bean cake hydrolyzate 30g / L, vitamin H 150μg / L, vitamin B11mg / L, pH 6.5-7.0; sterilized at 121°C for 30 minutes and cooled to 37°C), at a pressure of 0.1MPa and an air volume of 0.3- 0.8vvm, dissolved oxygen 30-60% under the condition of fermentation culture. 13h after ...

Embodiment 2

[0022] Embodiment 2 improves the method for lysine fermentation yield

[0023] The seed tank medium for cassava liquefaction liquid culture is: cassava liquefaction liquid 100g / L, corn steep liquor 3g / L, KH 2 PO 4 ·3H 2 O 15g / L, MgSO 4 ·7H 2 O 0.4g / L, urea 4g / L, FeSO 4 ·7H 2 O 0.01g / L, MnSO 4 ·H 2 O 0.01g / L, the culture conditions used for seed tank culture are: inoculum size 1‰, temperature 37°C, ventilation ratio 1:0.5vvm, tank pressure 0.05Mpa, full control of DO30% or more, pH6.90, cycle 13h.

[0024] The seed tank seed liquid cultivated with cassava liquefaction liquid is inserted into the fermenter culture medium (cassava liquefaction liquid 140g / L, ammonium sulfate 50g / L, KH 2 PO 4 ·3H 2 O 4.5g / L, MgSO 4 ·7H 2 O 0.5g / L, FeSO 4 ·7H 2 O 0.015g / L, MnSO 4 ·H2 O 0.015g / L, bean cake hydrolyzate 30g / L, vitamin H 150μg / L, vitamin B11mg / L, pH 6.5-7.0; sterilized at 121°C for 30 minutes and cooled to 37°C), at a pressure of 0.1MPa and an air volume of 0.3- 0.8vvm,...

Embodiment 3

[0025] Embodiment 3 improves the method for lysine fermentation yield

[0026] The seed tank medium for cassava liquefaction liquid culture is: cassava liquefaction liquid 100g / L, corn steep liquor 3g / L, KH 2 PO 4 ·3H 2 O 15g / L, MgSO 4 ·7H 2 O 0.4g / L, urea 4g / L, FeSO 4 ·7H 2 O 0.01g / L, MnSO 4 ·H 2 O 0.01g / L, the cultivation conditions adopted for primary seed tank cultivation are: inoculum size 1‰, temperature 37°C, ventilation ratio 1:0.5vvm, tank pressure 0.05Mpa, full control of DO30% or more, PH6.90, cycle 13h.

[0027] The seed tank seed liquid cultivated with cassava liquefaction liquid is inserted into the fermenter culture medium (cassava liquefaction liquid 140g / L, ammonium sulfate 50g / L, KH 2 PO 4 ·3H 2 O 4.5g / L, MgSO 4 ·7H 2 O 0.5g / L, FeSO 4 ·7H 2 O 0.015g / L, MnSO 4 ·H 2 O 0.015g / L, bean cake hydrolyzate 30g / L, vitamin H 150μg / L, vitamin B11mg / L, pH 6.5-7.0; sterilized at 121°C for 30 minutes and cooled to 37°C), at a pressure of 0.1MPa and an air v...

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PUM

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Abstract

The invention provides a novel method for enhancing fermentation yield of lysine, which comprises the following steps: by using Corynebacterium glutamicum as a lysine fermentation strain, converting the glucose in the seed tank culture medium and fermentation tank culture medium into equivalent cassava liquefaction solution, and carrying out fermentation culture under the conditions of feeding a carbon source, a nitrogen source and minor elements. The cheap accessible cassava is used as the raw material, and the liquefaction substance of the cassava is used as the carbon source instead of the glucose in the lysine seed tank and fermentation tank culture medium, thereby enhancing the lysine yield, increasing the conversion rate, reducing the raw material expenses and further lowering the cost.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for increasing the yield of lysine fermentation. Background technique [0002] Lysine is one of the essential amino acids for the human body, which can promote human development, enhance immune function, and improve the function of central nervous tissue. Lysine is a basic essential amino acid. Since the content of lysine in grain food is very low, and it is easily destroyed and lacked during processing, it is called the first limiting amino acid. [0003] Lysine is an amino acid needed by the human body, an indispensable nutrient. As we all know, protein is the main component of human cells. After entering the human body, protein in food is first decomposed into amino acids after digestion, and then the human body uses these amino acids to synthesize new human proteins, such as immune antibodies, digestive enzymes, plasma proteins, and growth hormones. etc. are ...

Claims

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Application Information

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IPC IPC(8): C12P13/08C12R1/15
Inventor 尚海涛徐斌杨为华邓远德戴嘉张雪锋穆晓玲
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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