Method for producing high-lysine fermented feed through fermentation of vinegar residue using two-step method

A high-lysine, fermented feed technology, applied in animal feed, animal feed, application, etc., can solve the problems of unbalanced amino acid composition, difficult comprehensive utilization, unfavorable environmental protection, etc., to improve amino acid composition and intestinal microflora. Ecology, the effect of improving the added value of products

Inactive Publication Date: 2010-06-30
TIANJIN SHENGJI GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the perspective of nutrition, vinegar grains are not an ideal feed protein source, the protein content is low, and the composition of amino acids is unbalanced. There is also a process to extract effective substances such as protein from vinegar grains, but the efficiency is low and the cost is high
The remaining substances are sti

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for producing high-lysine fermented feed by fermenting vinegar grains in two steps, comprising the following steps:

[0020] (1) Prepare potato culture medium, take 20% of potatoes by mass, 2% of sucrose, and the balance is water, and sterilize at a temperature of 121°C for 13 minutes; inoculate Aspergillus niger strains into potato culture medium to make the The Aspergillus niger species accounted for 2% of the quality of the potato culture medium, cultivated under ventilated conditions for 32 hours at 28°C;

[0021] (2) prepare lysine seed culture medium, take glucose 2.0% by mass, (NH 4 ) 2 SO 4 0.4%, urea 0.1%, K 2 HPO 4 0.1%, corn steep liquor 2%, soybean cake hydrolase 2%, MgSO 4 0.025%, the balance is water, adjust the pH to be 7.0, and sterilize at 0.1Mpa for 15min; Corynebacterium glutamicum bacterial classification is inoculated in the lysine seed culture medium, makes described Corynebacterium glutamicum bacterial classification occupy lysine ...

Embodiment 2

[0026] A method for producing high-lysine fermented feed by fermenting vinegar grains in two steps, comprising the following steps:

[0027] (1) Prepare potato culture medium, take by weight 20% of potatoes, 2% of sucrose, and the balance is water, and sterilize at 121°C for 12 minutes; inoculate Aspergillus niger strains into potato culture medium, and make the The above-mentioned Aspergillus niger species accounted for 5% of the quality of the potato culture medium, cultivated under ventilated conditions for 18 hours at 35°C;

[0028] (2) prepare lysine seed culture medium, take glucose 2.0% by mass, (NH 4 ) 2 SO 4 0.4%, urea 0.1%, K 2 HPO 4 0.1%, corn steep liquor 2%, soybean cake hydrolase 2%, MgSO 4 0.025%, the balance is water, adjust the pH to be 7.0, and sterilize at 0.1Mpa for 15min; Corynebacterium glutamicum bacterial classification is inoculated in the lysine seed culture medium, makes described Corynebacterium glutamicum bacterial classification occupy lysin...

Embodiment 3

[0033] A method for producing high-lysine fermented feed by fermenting vinegar grains in two steps, comprising the following steps:

[0034] (1) Prepare potato culture medium, take by weight 20% of potatoes, 2% of sucrose, and the balance is water, and sterilize at 121°C for 10 minutes; inoculate Aspergillus niger strains into potato culture medium to make the The above-mentioned Aspergillus niger species accounted for 3% of the quality of the potato culture medium, cultivated under ventilated conditions for 48 hours at 25°C;

[0035] (2) prepare lysine seed culture medium, take glucose 2.0% by mass, (NH 4 ) 2 SO 4 0.4%, urea 0.1%, K 2 HPO 40.1%, corn steep liquor 2%, soybean cake hydrolase 2%, MgSO 4 0.025%, the balance is water, adjust the pH to be 7.0, and sterilize at 0.1Mpa for 15min; Corynebacterium glutamicum bacterial classification is inoculated in the lysine seed culture medium, makes described Corynebacterium glutamicum bacterial classification occupy lysine ...

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PUM

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Abstract

The invention discloses a method for producing a high-lysine fermented feed through fermentation of vinegar residue using a two-step method, comprising the following steps: (1) preparing potato culture medium, inoculating aspergillus niger strains onto the potato culture medium to cultivate; (2) preparing lysine seed culture medium; inoculating corynebacterium glutamicum strains onto the lysine seed culture medium to cultivate; (3) inoculating the aspergillus niger strains onto the vinegar residue to ferment and obtain a pretreatment material through the fermentation of the vinegar residue by the aspergillus niger; (4) inoculating the corynebacterium glutamicum strains onto the pretreatment material which is fermented by the aspergillus niger to ferment and cultivate; and (5) drying at a temperature not higher than 85 DEG C until the water content is below 15% so as to obtain the high-lysine fermented feed through the fermentation of the vinegar residue. The feed produced through the method of the invention has high lysine content and a variety of microbial enzymes, etc. The substances has obvious effects on digestion and absorption, growth and development, and body immunity of animals, and can increase beneficial bacteria in animal intestines and improve intestinal microecology.

Description

technical field [0001] The invention relates to a microbial solid-state fermentation technology, in particular to a two-step method for fermenting vinegar grains to produce high-lysine fermented feed. Background technique [0002] Vinegar grains are the largest by-product of vinegar production. At present, the whereabouts of vinegar grains are mainly used as feed for aquaculture. Vinegar grains can be directly used as animal feed, and these feeds are often directly fed to various animals after simple crushing, cooking and other processing. In this way, the utilization rate of active ingredients in the feed is low, causing a lot of waste. Moreover, long-term consumption of such feed by animals will cause malnutrition and low immunity. Slow down the growth rate of the animal or reduce the egg production rate of the animal. From the perspective of nutrition, vinegar grains are not an ideal feed protein source, with low protein content and unbalanced amino acid composition. ...

Claims

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Application Information

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IPC IPC(8): A23K1/06A23K10/38
CPCY02P60/87
Inventor 田杏芳王连民
Owner TIANJIN SHENGJI GRP CO LTD
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