Method for continuously fermenting lysine

A technology of lysine fermentation and lysine, which is applied in the field of lysine fermentation, can solve the problems of low use efficiency, achieve the effect of prolonging the time, increasing the amount of acid supply, and improving the metabolic capacity

Inactive Publication Date: 2012-07-04
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a new method for lysine fer...

Method used

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  • Method for continuously fermenting lysine

Examples

Experimental program
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Effect test

Embodiment 1

[0057] This example is used to illustrate the preparation method of lysine provided by the present invention.

[0058] (1) Machining 100 parts by weight of harvested corn to pulverize corn grains, so that the passing rate of corn flour through a 30-mesh sieve is 80%.

[0059] (2) Add water to the pulverized product to 12Bé°, add 20 enzyme activity units of amylase (Novozymes, α-amylase) relative to the dry weight of each gram of the pulverized product, and heat it at 90°C, Enzymolysis was carried out for 100 minutes under the condition of pH 5.5 to obtain an enzymolysis product. Wherein, the enzymolysis product is filtered through a hydraulic plate and frame filter press, and the enzymolysis clear liquid (solid content is 20% by weight) is separated; 115 enzyme activity units of glucoamylase (α-1, 4 -glucose hydrolase, Novozymes), enzymolysis at 60° C. and pH 4.5 for 420 minutes to obtain saccharified supernatant liquid of starchy raw materials.

[0060] (3) use the sacchari...

Embodiment 2

[0065] This example is used to illustrate the preparation method of lysine provided by the present invention.

[0066] The preparation method of starchy raw material saccharification liquid, the formula of seed tank medium, the method of culturing mature seed liquid in seed tank, and the formula of fermenter medium are all the same as in Example 1.

[0067] The medium volume in the fermentor was 40% of the fermentor volume. The mature seed liquid is inserted into the culture medium of the fermenter for fermentation and cultivation, and the inoculum amount of the seed liquid is 12% by volume based on the inoculated fermentation medium. After the inoculation, the obtained starchy raw material saccharification liquid and ammonium sulfate were fed continuously, and the amount of the obtained starchy raw material saccharified liquid was fed to control the concentration of reducing sugar in the fermentation broth at 5-7 grams per liter. Add the amount of ammonium sulfate to control...

Embodiment 3

[0070] This example is used to illustrate the preparation method of lysine provided by the present invention.

[0071] The preparation method of starchy raw material saccharification liquid, the formula of seed tank medium, the method of culturing mature seed liquid in seed tank, and the formula of fermenter medium are all the same as in Example 1.

[0072] The medium volume in the fermentor was 60% of the fermentor volume. The mature seed liquid is inserted into the culture medium of the fermenter for fermentation and cultivation, and the inoculum amount of the seed liquid is 18% by volume based on the inoculated fermentation medium. After the inoculation, the obtained starchy raw material saccharification clear liquid and ammonium sulfate were fed continuously, and the amount of the obtained starchy raw material saccharified clear liquid was fed to control the concentration of reducing sugar in the fermentation broth at 8-10 grams per liter. Add the amount of ammonium sulfa...

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Abstract

The invention discloses a method for continuously fermenting lysine, which comprises the following steps: inoculating a lysine fermentation strain into a lysine fermentation medium; under the conditions of a flow-feed carbon source and a flow-feed nitrogen source, and carrying out fermentation and culture. The invention is characterized in that nutrient substances required by lysine fermentation and culture are added into the fermentation liquid 20 hours after the fermentation and culture are started. The method disclosed by the invention greatly prolongs the fermentation and culture time, enhances the metabolic capability of the lysine strain, can carry out fermentation all along under the conditions that the equipment does not goes wrong and the fermentation medium is not contaminated by bacteria, increases the acid supply amount per unit time, and enhances the equipment utilization rate, thereby lowering the lysine fermentation cost.

Description

technical field [0001] The invention relates to a lysine fermentation method, in particular to a lysine continuous fermentation method. Background technique [0002] At present, my country's lysine fermentation adopts the method of batch fermentation. The fermentation uses a certain proportion of each tank to put into the fermentation medium and insert a certain lysine fermentation strain. Under the conditions of feeding carbon source and nitrogen source , for fermentation. As the fermentation progressed, the acidity of lysine increased gradually, the total volume increased, the concentration of nutrients decreased gradually, and the rate of acid production by fermentation showed a downward trend. When the nutrients are reduced to a certain level, the fermentation acid production rate is reduced to a certain level, which is judged as the end point of the fermentation, and the extraction process is entered after the fermentation is completed. [0003] As the fermentation pro...

Claims

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Application Information

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IPC IPC(8): C12P13/08C12R1/13C12R1/15C12R1/19
Inventor 陈修卢宗梅吴晓艳钟华邵引刚
Owner COFCO BIOTECHNOLOGY CO LTD
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