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Method for improving poly-malic acid yield

A technology of polymalic acid and yield, applied in the field of biochemistry, can solve the problems that the influence of polymalic acid yield has not been reported, and achieve the effects of high yield, increased yield, and low fermentation cost

Active Publication Date: 2014-05-14
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Prior art reports that 0.1%, 0.5% and 1% Tween-80 can improve the ability of Aureobasidium pullulan to produce pullulan, but the impact of Tween on the yield of polymalic acid has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Aureobasidium pullulans with the preservation number CCTCC NO: M2012223 was inoculated in a shake flask containing 50 mL of seed medium (the volume of the shake flask was 500 mL), and cultured for 48 hours at a temperature of 25 ° C and a rotation speed of 220 rpm Then take the culture solution and inoculate it in a 500mL shake flask according to the inoculum size of 10%. The shake flask contains 50mL of the fermentation medium containing 0.1g / L Tween-80, and then the temperature is 25°C and the rotation speed is 220rpm. 96 hours; then detect the content of polymalic acid and pullulan in the culture medium. The results showed that the yield of polymalic acid in this example was 25.69g / L, and the yield of pullulan was 13.99g / L.

[0025] At the same time, a comparative test was carried out according to the same method as above, except that Tween-80 was not added to the fermentation medium, and the contents of polymalic acid and pullulan in the culture medium were detected...

Embodiment 2

[0030] Aureobasidium pullulans with the preservation number CCTCC NO: M2012223 was inoculated into a shake flask containing 50 mL of seed medium (the volume of the shake flask was 500 mL), and cultured at a temperature of 25°C and a rotation speed of 220 rpm for 48 hours; Then take the culture solution and inoculate it into a 500mL shake flask according to the inoculum size of 10%. hours; detect the content of polymalic acid and pullulan in the culture medium. The results showed that the yield of polymalic acid was 25.84g / L, and that of pullulan was 13.69g / L.

[0031] Simultaneously carry out comparative test according to the same method as above, the difference is that Tween-80 is not added in the fermentation medium, and the content of polymalic acid and pullulan in the detection culture fluid after fermentation is finished, the content of polymalic acid in this comparative example The yield was 21.95g / L, and the yield of pullulan was 23.04g / L.

[0032] Compared with the c...

Embodiment 3

[0036] Aureobasidium pullulans with the preservation number CCTCC NO: M2012223 was inoculated in a shake flask containing 50 mL of seed medium (the volume of the shake flask was 500 mL), and cultured for 48 hours at a temperature of 25 ° C and a rotation speed of 220 rpm Then get the culture solution and inoculate it in a 500mL shake flask according to the inoculum size of 10%. The shake flask contains 50mL of fermentation medium containing 0.3g / L Tween-40, and then the temperature is 25°C and the rotation speed is 220rpm. 96 hours; then detect the content of polymalic acid and pullulan in the culture medium. The results showed that the yield of polymalic acid was 25.39g / L, and the yield of pullulan was 15.53g / L.

[0037] At the same time, a comparative test was carried out according to the same method as above, except that Tween-40 was not added to the fermentation medium, and the contents of polymalic acid and pullulan in the culture medium were detected after fermentation. ...

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PUM

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Abstract

The invention discloses a method for improving the poly-malic acid yield in aureobasidium pullulans through surface active agent tween or span. The method particularly comprises the steps that after the aureobasidium pullulans is activated, the aureobasidium pullulans is fermented under the condition containing 0.1 g / L to 0.5 g / L tween or span. The method is simple, the tween or the span can be directly added into a fermentation medium, the carbon metabolism flow direction of synthesis of the poly-malic acid and Pulullan can be adjusted, the poly-malic acid yield can be improved to a large extent, and the method has the advantages of being low in fermentation cost, high in yield, strong in technical economical efficiency, capable of being applied to poly-malic acid industrial production and the like.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a method for increasing the yield of polymalic acid by using surfactant Tween or Span. Background technique [0002] Aureobasidum pullulan is a kind of yeast fungus, which has two forms of mycelium and yeast-like. This species exists widely in nature. Polymalic acid and pullulan were the main polymer products fermented by Aureobasidium pullulans. [0003] Polymalic acid (Polymalic acid) is polymerized in vivo with L-malic acid as the only monomer. It is a new type of fully biodegradable polymer material with high biocompatibility, biodegradability and bioabsorbability. Etc. It can be used in drug sustained and controlled release carriers, food processing and other fields; Pullulan is an extracellular water-soluble mucopolysaccharide similar to dextran and xanthan gum, and can be used as a thickener and coating in the food field agent. [0004] Usually, two macromolecu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12P19/10C12R1/645
Inventor 邹祥涂光伟王永康
Owner SOUTHWEST UNIV
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