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Preparation method and application of lactobacillus plantarum for feed

A Lactobacillus plantarum and feeding technology, applied in the application, animal feed, animal feed and other directions, can solve problems such as difficulty in further improvement, and achieve the effects of low fermentation cost, short cycle and low energy consumption

Inactive Publication Date: 2013-07-03
北京资源亚太饲料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional carbon and nitrogen source optimization method can make Lactobacillus plantarum reach 10 9 After CFU / ml, it is difficult to further increase the content of carbon and nitrogen sources, indicating that the traditional carbon and nitrogen sources have satisfied its growth

Method used

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  • Preparation method and application of lactobacillus plantarum for feed
  • Preparation method and application of lactobacillus plantarum for feed
  • Preparation method and application of lactobacillus plantarum for feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 The number of viable bacteria in the fermentation process of Lactobacillus plantarum (figure is shown in the accompanying drawing of the description)

[0023] The number of viable bacteria in the fermentation broth of Lactobacillus plantarum (see the attached figure in the manual)

Embodiment 2

[0024] Example 2 Application of Lactobacillus plantarum in weaned piglets

[0025] 1. Materials and methods

[0026] 1.1 Experimental animals and grouping: three-way hybrid pigs (male parent: Duroc, female parent: growing up), 40 weaned piglets at the age of 28 days, were randomly divided into two groups, each group had 2 replicates, and each replicated 10 pigs. Half male and half female. 1000ppm Lactobacillus plantarum was added to the feed of piglets in the test group; the control group was given a blank diet. The feed is self-prepared with 4% premix (without antibiotics), and the formula is made by resource formulator software

[0027] 1.2 Test product Lactobacillus plantarum

[0028] 1.3 Test time and place

[0029] Test time: June 9, 2010 - July 9, 2010

[0030] Test location: Beijing Resources Asia-Pacific Breeding Pig Co., Ltd.

[0031] 1.4 Test method

[0032] Feeding and management: breeding in pens, ad libitum feeding, free drinking water, good ventilation, va...

Embodiment 3

[0046] Application of embodiment 3 lactobacillus plantarum in poultry

[0047] 1. Materials and methods

[0048] 1.1 Test animals and grouping Test animals and diets Test chickens use 1-day-old AA commercial meat substitute male-female mixed chicks '1000; test diets refer to the National Research Council Standards (NRC, 1994) and AA Chicken Feeding Standards. With feed.

[0049] 1.2 Test product Lactobacillus plantarum

[0050] 1000 test AA broiler chickens were randomly divided into 5 groups, 200 in each group, 5 repetitions, 40 in each repetition, free to drink water and feed. Control group: blank control group, fed with basal diet; antibiotic group: basal diet+antibiotics (10mg / kg): 0, 0.01%, 0.05%, 0.1% Lactobacillus plantarum were added to the basal diet.

[0051] 2. Results and discussion

[0052] 2.1 The test results are shown in the table below

[0053]

[0054]

[0055] The same lowercase letters in the same column indicate no significant difference (P>0.05...

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Abstract

The invention provides a preparation method for lactobacillus plantarum used for a feed. To overcome the problems of abuse of antibiotics in farming, generation of drug resistance and the like at present, application of a novel antibiotic substitute product, i.e., probiotics, is a trend of modern farming. The probiotics is prepared through deep liquid state fermentation, has the advantages of low production cost and a high viable count and is a stable, highly-efficient and green feed additive.

Description

technical field [0001] The invention relates to a preparation method and application of a green feed additive probiotic Lactobacillus plantarum. technical background [0002] The application of antibiotics has produced huge economic benefits in animal husbandry. The survey shows that the application of antibiotics in animal husbandry can increase pork production by 4%. However, the negative effects associated with the extensive use of antibiotics are becoming more and more prominent, mainly manifested in the emergence of drug resistance of pathogenic microorganisms and drug residues in animals, thus causing direct or potential harm to human health. The application of antibiotics in feed encounters severe challenges. Therefore, the research and development of antibiotic substitutes (Lactobacillus plantarum and acid preparations, etc.) has become a hot spot in green feed additives in recent years, and the application of Lactobacillus plantarum has attracted much attention. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/17A23K20/195
Inventor 莫少华张江王晓娟
Owner 北京资源亚太饲料科技有限公司
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