Lysine preparing method

A technology for lysine and lysine production, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as low fermentation efficiency, and achieve the effects of improving fermentation efficiency and reducing production costs.

Active Publication Date: 2012-07-11
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the defects of low lysine production and fermentation efficiency existing in the prior art, and provide a method for preparing lysine with high fermentation efficiency

Method used

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  • Lysine preparing method

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preparation example Construction

[0010] The invention provides a method for preparing lysine. The method comprises: inoculating lysine-producing microorganisms into fermentation broth and fermenting to produce lysine. During the fermentation process, according to the amount of carbon source and nitrogen source in the fermentation broth , adding carbon source and / or nitrogen source, wherein, the process of fermenting and producing lysine includes: the rising stage of the number of lysine-producing microorganisms, the first decline stage of the number of lysine-producing microorganisms, the increase of the number of lysine-producing microorganisms The maintenance stage and the second decline stage of the number of lysine-producing microorganisms; wherein, in the rising stage of the number of lysine-producing microorganisms, the ventilation rate increases with the increase of the fermentation broth volume; During the first decline phase and the maintenance phase of the lysine-producing microbial population, the a...

Embodiment 1

[0043] This example is used to illustrate the preparation method of lysine provided by the present invention.

[0044] (1) Machining 100 parts by weight of harvested corn to pulverize corn grains, so that the passing rate of corn flour through a 30-mesh sieve is 80%.

[0045] (2) Add water to the pulverized product to 12Be°, add 20 enzyme activity units of amylase (Novozymes, a-amylase) relative to the dry weight of each gram of the pulverized product, and heat it at 85°C, Enzymolysis was carried out for 100 minutes under the condition of pH 5.5 to obtain an enzymolysis product. Wherein, the enzymolysis product is filtered by a hydraulic plate and frame filter press, and the enzymolysis clear liquid (solid content is 20% by weight) is separated; 115 enzyme activity units of glucoamylase (α-1, 4 - Glucose hydrolase, Novozymes), enzymolysis at 60°C and pH 4.5 for 420 minutes to obtain saccharification serum A1 of starchy raw materials.

[0046] (3) Use the starchy raw material...

Embodiment 2

[0050] This example is used to illustrate the preparation method of lysine provided by the present invention.

[0051]Prepare lysine according to the method for embodiment 1, difference is: the initial total volume of fermented liquid is 120 cubic meters, and initial ventilation is 30 cubic meters of air / minute (the unit ventilation is 0.67 cubic meters of air / cubic meter broth / min). The tank pressure is 0.08MPa, the fermentation temperature is controlled at 36°C, and the pH is adjusted with liquid ammonia to maintain it at 6.8. During the fermentation process, the number of lysine-producing microorganisms was measured several times, and the two adjacent values ​​were compared to determine the stage of fermentation (from the 0th hour to the 14th hour, every 30 minutes, and the 14th hour From then on to the 24th hour, check once every 60 minutes, from the 24th hour to the 48th hour, check once every 120 minutes, from the 48th hour to the end of fermentation, check once every 6...

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Abstract

The invention provides a lysine preparing method which ferments the lysine through the product of dry sugar manufacturing, divides the fermenting process into four steps, namely, increasing step of the number of lysine-producing microbe, first decreasing step of the number of lysine-producing microbe, retaining step of the number of lysine-producing microbe and second decreasing step of the number of lysine-producing microbe, adopts respective ventilation control, so as to enable the lysine-producing microbe to better produce the lysine, improve the fermenting efficiency of the lysine, and reduce the production cost of the lysine. Furthermore, as the control of the fermentation process is adjusted, the product of the dry sugar manufacturing can effectively ferment, so as to highlight the low cost of the dry sugar manufacturing, and integrally improve the economy of the preparing technology of the lysine.

Description

technical field [0001] The invention relates to a preparation method of lysine. Background technique [0002] L-lysine (hereinafter referred to as lysine) is one of the eight essential amino acids for human and animal nutrition. It plays an important role in regulating the metabolic balance in the body, improving the absorption of cereal protein in the body, improving human dietary nutrition and animal nutrition, and promoting growth and development. [0003] At present, it is mainly used in medicine, food and feed industry. From the perspective of consumption structure, the consumption of lysine in feed accounts for nearly 90%, while the consumption of food and pharmaceutical intermediates only accounts for 10%. [0004] Most lysine is produced by microbial fermentation. Generally, lysine-producing microorganisms are inoculated into the fermentation broth, and air is continuously introduced to ferment lysine. According to the carbon and nitrogen sources in the fermentation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08C12R1/19C12R1/15C12R1/13
Inventor 周永生卢宗梅郭翠钟华
Owner COFCO BIOTECHNOLOGY CO LTD
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