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Production method of lysine

A production method, lysine technology, applied in the field of lysine production, can solve the problems of limited lysine production capacity, low economic benefits, slow rate, etc., to increase the end-point lysine content, improve economic benefits, reduce The effect of production costs

Inactive Publication Date: 2012-07-04
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the fermentation proceeds, and with the addition of carbon and nitrogen sources and feeding during the fermentation process, the concentration of the lysine fermenting strains is gradually diluted and the amount of the lysine fermenting strains gradually decreases, and the fermentation yield The acid rate showed a downward trend, and finally the tank was released due to the slow rate of fermentation acid production, which limited the production capacity of lysine, high cost and low economic benefit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] This example is used to illustrate the production method of lysine provided by the present invention.

[0058] (1) Machining 100 parts by weight of harvested corn to pulverize corn grains, so that the passing rate of corn flour through a 30-mesh sieve is 80%.

[0059](2) Add water to the pulverized product to 12Bé°, add 20 enzyme activity units of amylase (Novozymes, α-amylase) relative to the dry weight of each gram of the pulverized product, at 90°C, Enzymolysis was carried out for 100 minutes under the condition of pH 5.5 to obtain an enzymolysis product. Wherein, the enzymolysis product is filtered by a hydraulic plate and frame filter press, and the enzymolysis clear liquid (solid content is 20% by weight) is separated; 115 enzyme activity units of glucoamylase (α-1, 4 -glucose hydrolase, Novozymes), enzymolysis at 60° C. and pH 4.5 for 420 minutes to obtain saccharified supernatant liquid of starchy raw material.

[0060] (3) use the saccharification clear liqui...

Embodiment 2

[0065] This example is used to illustrate the production method of lysine provided by the present invention.

[0066] The preparation method of starchy raw material saccharification liquid, the formula of seed tank medium, the method of culturing mature seed liquid in seed tank, and the formula of fermenter medium are all the same as in Example 1.

[0067] The medium volume in the fermentor was 40% of the fermentor volume. The mature seed liquid is inserted into the culture medium of the fermenter for fermentation and cultivation, and the inoculum amount of the seed liquid is 12% by volume based on the inoculated fermentation medium. After the inoculation, the obtained starchy raw material saccharification liquid and ammonium sulfate were fed continuously, and the amount of the obtained starchy raw material saccharified liquid was fed to control the concentration of reducing sugar in the fermentation broth at 5-7 grams per liter. Add the amount of ammonium sulfate to control ...

Embodiment 3

[0070] This example is used to illustrate the production method of lysine provided by the present invention.

[0071] The preparation method of starchy raw material saccharification liquid, the formula of seed tank medium, the method of culturing mature seed liquid in seed tank, and the formula of fermenter medium are all the same as in Example 1.

[0072] The medium volume in the fermentor was 60% of the fermentor volume. The mature seed liquid is inserted into the culture medium of the fermenter for fermentation and cultivation, and the inoculum amount of the seed liquid is 18% by volume based on the inoculated fermentation medium. After the inoculation, the obtained starchy raw material saccharification clear liquid and ammonium sulfate were fed continuously, and the amount of the obtained starchy raw material saccharified clear liquid was fed to control the concentration of reducing sugar in the fermentation broth at 8-10 grams per liter. Add the amount of ammonium sulfat...

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PUM

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Abstract

The invention discloses a production method of lysine. The method comprises the step of inoculating lysine fermentation strains into lysine fermentation media for fermentation culture under the condition of feeding a carbon source and a nitrogen source. The method is characterized by refilling lysine fermentation strains into fermentation liquor within 30-48 hours of fermentation culture. The method has the following advantages: the fermentation time can be lengthened and the content of final lysine, the acid supply quantity per tank and the conversion rate can be improved, thus lowering the production cost and improving the economic benefits; and by adopting the optimized embodiment, namely refilling the lysine fermentation strains extracted from the emptied fermentation liquor within 15-40 hours of fermentation culture, the lysine fermentation strains in the emptied fermentation liquor can be fully utilized, thus further lowering the production cost and improving the economic benefits.

Description

technical field [0001] The invention relates to a production method of lysine. Background technique [0002] Lysine is mostly prepared by microbial fermentation. Each tank is put into the fermentation medium in a certain proportion and inserted into a certain lysine fermentation strain. Under the conditions of feeding carbon source and nitrogen source, the Fermentation culture. As the fermentation proceeds, and with the addition of carbon and nitrogen sources and feeding during the fermentation process, the concentration of the lysine fermenting strains is gradually diluted and the amount of the lysine fermenting strains gradually decreases, and the fermentation yield The acid rate showed a downward trend, and finally the tank was released due to the slow rate of fermentation acid production, which limited the production capacity of lysine, high cost and low economic benefit. Contents of the invention [0003] The purpose of the present invention is to provide a new prod...

Claims

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Application Information

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IPC IPC(8): C12P13/08C12R1/13C12R1/15C12R1/19
Inventor 熊结青卢宗梅张继学章辉平朱继成
Owner COFCO BIOTECHNOLOGY CO LTD
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