Method for producing l-tryptophan by bacterial fermentation with altered tdcd enzyme regulatory elements

Abstract

The invention provides a method for fermentatively producing L-tryptophan, which comprises transforming the bacterial chromosome wxya The wild-type regulatory element of the gene, so that the regulatory element of the transformed bacterium has its downstream enzyme gene (such as, wxya The ability to initiate expression of the gene) is weakened but not disappeared; and, L-tryptophan is produced by fermentation with the transformed bacteria. In addition, the present invention also provides methods and uses derived from this method, as well as polynucleotides, vectors and bacteria that can be used in these methods and applications.

Description

technical field [0001] The present invention belongs to the field of amino acid fermentation, in particular, the present invention relates to a method for fermentative production of l-tryptophan and its derivation method and application, as well as polynucleotides, vectors and bacteria that can be used in these methods and applications. Background technique [0002] Production of l-tryptophan by fermentation of l-tryptophan-producing bacteria (eg, Escherichia coli of the genus Escherichia and rod-shaped bacteria of the genus Corynebacterium) has been industrially applied. These bacteria can be bacteria isolated from nature, bacteria obtained through mutagenesis or genetic engineering, or both. In the current literature reports, the attention through genetic engineering is mainly focused on mtr, aroP, tnaA, trpB, gnd, and traB In terms of isogenes (see Chinese patents 85101270, 93117586, 96111972, 200580043246, 200710056966, 201010598350, etc.), no attention has been paid to...

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Problems solved by technology

In the current literature reports, the attention through genetic engineering is mainly focused on mtr, aroP, tnaA, trpB, gnd, and traB In terms of isogenes (see Chinese patents 85101270, 93117586, 96111972, 200580043246, 200710056966, 201010598350, etc.), no attention has been paid to the tdcD enzyme and its coding gene for the production of L-tryptophan, let alone the modification of the regulatory elements of the tdcD enzyme , in fact the international patent WO2012135389 discloses the production of oxidation products of aromatic amino acids, which even requires the additional expression of heterologous tdc enzymes