289 results about "L-threonine" patented technology

There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.

L-threonine or L-isoleucine is produced by culturing a bacterium which belongs to the genus Escherichia and has an ability to produce L-threonine or L-isoleucine, and wherein expression of a threonine operon is directed by its native promoter, and from which at least a leader sequence and an attenuator are deleted, in a medium and collecting the L-threonine or L-isoleucine from the medium.

PCT No. PCT / JP96 / 02503 Sec. 371 Date Mar. 2, 1998 Sec. 102(e) Date Mar. 2, 1998 PCT Filed Sep. 4, 1996 PCT Pub. No. WO97 / 08946 PCT Pub. Date Mar. 13, 1997The present invention aims to provide a medium composition for in vitro fertilization, in particular, a composition usable in the culture of ova or early embryos which are fertilized eggs, the preparation or culture of sperm, and the pre-treatment of ova or sperm. The composition comprises, as its essential components, L-phenylalanine, L-tryptophan, L-lysine, L-threonine, L-valine, L-methionine, L-isoleucine, L-leucine, L-proline, glycine, L-alanine, L-tyrosine, L-histidine, L-arginine, L-taurine, L-aspartic acid, L-serine, L-asparagine, L-glutamic acid, L-glutamine and L-cystine, provided that at least a part of the L-cystine may be replaced by L-cysteine.

Provided are compositions and methods for increasing diet induced thermogenesis. Typically, the compositions are comprised of L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-valine and L-threonine.

The invention provides a low protein ration premix compound for growing pigs and a using method thereof. The low protein ration premix compound consists of mountain flour, calcium hydrophosphate, salt, sodium bicarbonate, L-lysine hydrochloride, DL-methionine, L-threonine hydrochloride, L-tryptophan hydrochloride, an antioxidant and the like. The crude protein content of the prepared growing and fattening pig ration is respectively reduced from 16.4 percent to 14.5 percent and from 15.4 percent to 12.5 percent. The invention aims to add commodity amino acid to produce the low protein ration premix compound to enlarge the applicability of the low protein ration technology and improves the conversion efficiency of the ration, reduces the use of the protein raw materials, reduces the feeding cost and reduces discharge by reducing the concentration of the crude protein of the prepared ration.

The invention discloses a special gestation compound feed for sows at later stage of pregnancy. The special gestation compound feed is prepared from the following raw materials: corn, bran, rice bran meal, DDGS (dried distiller grains with soluble), swelling soya dregs, swelling soybean flour, fermented soybean meal, compound sugar, mountain flour, calcium hydrogen phosphate, choline chloride, common salt, compound premix, L-lysine hydrochloride, L-threonine, acidifier, clostridium butyricum and yeast cell wall polysaccharides. The invention, starts from nutrition of the sows at the later stage of the pregnancy, gives consideration to the growth of fetuses and the physiological characteristics of the sows per se, designs daily ration with high protein, high amino acid and low energy, adds the compound sugar constituted by different monosaccharides and disaccharides, and can provide energy which can be utilized quickly and improve the birth weight and the uniformity of piglets; and by adding the clostridium butyricum and the yeast cell wall polysaccharides, the special gestation compound feed can improve the intestinal health of the sows, improve immunity, regulate intestinal peristalsis, reduce the constipation incidence of the sows, shorten the labor stage of the sows, reduce the incidence rate of mastitis and endometritis of the sows, and assist the sows in rapid postpartum recovery.

In its several embodiments, this invention discloses a pharmaceutical formulation comprising at least one antineoplastic agent or a pharmaceutically acceptable salt thereof, and at least one dissolution enhancing agent sufficient to substantially dissolve said at least one antineoplastic agent in at least one aqueous diluent, wherein said dissolution enhancing agent is urea, L-histidine, L-threonine, L-asparagine, L-serine, L-glutamine or mixtures thereof; a lyophilized powder comprising said pharmaceutical formulation, and articles of manufacture thereof.

A method for producing an L-amino acid is described, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan, or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of a wild-type alcohol dehydrogenase encoded by the adhE gene or a mutant alcohol dehydrogenase which is resistant to aerobic inactivation.

Disclosed herein are a method of preparing an L-methionine production strain by overexpressing proteins involved in L-methionine biosynthesis in an L-threonine production strain, a strain prepared by the method, and a method of producing L-methionine using the strain.

There is disclosed a method for producing L-threonine using bacterium belonging to the genus Escherichia wherein the bacterium has been modified to enhance an activity of aspartate-β-semialdehyde dehydrogenase.

The present invention provides L-threonine genetic engineering production bacteria with the preservation number of CCTCC M 2015233. The constructed L-threonine genetic engineering production bacteria can implement effective accumulation of L-threonine in a fermented liquid in the fermentation process, L-threonine yield and sugar acid conversion rate can be improved, no any amino acid is added in the fermentation process, production cost is low, and the method has wide industrial application prospect.

The invention provides a method for preparing (2S, 3R)-p-methyl sulfone phenyl ethyl serinate from the cheap and easily available methylsulfonyl benzaldehyde and L-threonine under the action of transaldolase. The amino acid intermediate then undergoes ethyl esterification to obtain a target product (2S, 3R)-p-methyl sulfone phenyl ethyl serinate.

There is disclosed a method for producing an L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan, or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of N-acetylglucosamine permease encoded by the nagE gene.

The invention discloses a low-protein weanling piglet feed which comprises the following raw materials: corns, bean pulp, calcium hydrogen phosphate, dried porcine soluble, fermented bean pulp, fish meal, soybean oil, a premix, sodium chloride, phytase, a compound antioxidant, a compound acidifier, a compound enzyme preparation, a microecological preparation, limestone, L-lysine hydrochloride, L-threonine, DL-methionine, L-tryptophan and a compound traditional Chinese medicine preparation. According to the feed, the crude protein content of piglet full-price daily ration is reduced to 16% from 19%; the compound acidifier, the compound enzyme preparation, the microecological preparation and the compound traditional Chinese medicine preparation are added, so that the immunity of piglets is comprehensively improved and the use of antibiotic drugs is reduced; low-protein high-growth piglet ration is prepared, so that the misunderstanding to the feed consumption caused by farmers is eliminated, the low-protein ration formula technology is really popularized in local, the use of protein raw materials and the diarrhea of the piglets are reduced, and the cost and the N discharge are reduced.

The invention relates to piglet creep feed, including the following components: 53 to 58 parts of expended maize, 8 to 12 parts of whey powder, 14 to 16 parts of fermented soybean meal, 1.8 to 2.2 parts of bean oil, 2.7 to 3.4 parts of glucose, 4.5 to 5.5 parts of fish meal, 4.5 to 5.5 parts of blood plasma protein powder, 2.3 to 2.7 parts of intestinal membrane albumen powder, 0.1 to 0.15 parts of DL-methionine, 0.35 to 0.4 parts of lysine salt, 0.1 to 0.15 parts of L-threonine, 0.25 to 0.35 parts of compound organic acid, 0.15 to 0.25 parts of choline chloride, 0.08 to 0.15 parts of calcium propionate, 0.06 to 0.10 parts of copper sulfate, 0.022 to 0.026 parts of ferrous sulphate, 0.02 to 0.04 parts of ferrous fumarate, 0.03 to 0.04 parts of zinc sulfate, 0.014 to 0.018 parts of manganese sulfate, 0.004 to 0.008 parts of calcium iodate, 0.004 to 0.005 parts of sodium selenite and 0.03 to 0.05 parts of piglet multi-vitamin; and the total amount is 100 percent. The invention takes the palatability and the high digestion rate of the feed into consideration, increases the energy level of the feed, pays attention to the balance of effective amino acid and microelements, enhances the capability of disease resistance of piglet, effectively eliminates the ablactation stress of the piglet, and enables the piglet not to lose weight after ablactation and to smoothly pass the ablactation period, thereby increasing the survival rate.

A bacterium which belongs to the Enterobacteriaceae family and has an ability to produce an L-amino acid such as L-lysine, L-threonine and L-tryptophan and is modified to enhance glutamic acid decarboxylase activity is cultured in a medium to produce and accumulate the L-amino acid in the medium or cells of the bacterium. Then, the L-amino acid is collected from the medium or the cells.

The invention relates to big pig fattening compound feed for improving carcass quality, and the feed is prepared from the following components: corn, wheat, wheat middlings, rice bran meal, puffed soybean meal, fermented soybean meal, DDGS (distillers dried grains with soluble), soybean lecithin, soybean oil, zeolite powder, attapulgite, mountain powder for feed, edible salt, choline chloride, big pig compound premix, L-lysine hydrochloride, L-threonine, phytase, wheat enzyme, saccharin sodium salt and additive. The big pig fattening compound feed provided by the invention utilizes multiple raw materials for realizing nutrition complementary in the aspect of nutritional regulation, designs nutrient-balanced daily ration, and can be used for improving the feed utilization rate, reducing the feed conversion ratio by 0.08-0.2, regulating and controlling the pig meat quality by adopting combination of multiple functional additives, reducing the backfat thickness and improving the carcass quality.

The invention provides a constipation-resisting complete formula feed for sows at the latter half of gestation. The complete formula feed is prepared from the following components: 19%-21% of corn, 10%-12% of soybean meal, 1.9%-2.5% of soybean peel, 28%-32% of wheat, 8%-12% of alfalfa powder, 13%-18% of barley, 1.5%-1.8% of soybean oil, 3%-8% of rice bran, 0.4%-0.7% of L-lysine sulfate, 0.20%-0.40% of L-threonine, 0.15%-0.20% of glycine betaine, 1.5%-2.0% of calcium hydrophosphate, 0.85%-1% of calcium carbonate, 0.01%-0.05% of sodium butyrate, 0.3%-0.5% of table salt, 0.5% of a vitamin premix, 0.3% of a microelement premix, 0.03% of a compound enzyme preparation, 0.02% of high-temperature-resisting phytase and the balance being other auxiliary additives. According to the constipation-resisting complete formula feed, the digestible rate of the feed can be increased and the digestion load of intestinal tracts is reduced; and high-quality fiber components and micro-ecological preparations are added so that the excessive absorption of water, caused by the fact that the digestion function is reduced, is avoided and the constipation of the sows at the latter half of gestation can be reduced.

The invention relates to a granular fermentation-broth-based feed additive, comprising an L-amino acid selected from the group consisting of L-lysine, L-methionine, L-threonine, L-tryptophan, L-valine or mixtures of these, suitable for the preparation of feeds for animals kept in aquaculture, where the content of the L-amino acid or the mixtures thereof amount to 20% by weight of the total composition of the granular feed additive; where at least 70% by weight of the particles have a particle diameter of from >63 μm to <300 μm, where the biomass content amounts to ≧5% by weight and where the surface of the particles is fully or partially coated with an edible oil, wherein the L-amino acid is L-lysine, is present as a sulphate salt and the molar ratio of sulphate to L-lysine amounts to at least 0.5.

A method for producing an L-amino acid, such as L-histidine, L-threonine, L-lysine, L-glutamic acid, and L-tryptophan, using bacterium belonging to the genus Escherichia which has increased expression of genes, such as those of the xylABFGHR locus, which encode the xylose utilization enzymes, is disclosed. The method includes cultivating the L-amino acid producing bacterium in a culture medium containing xylose, and collecting the L-amino acid from the culture medium.

The invention discloses a preparation method for imipenem medicine intermediate 4AA. The preparation method comprises making 4-substituted aniline into an intermediate A, perform epoxidation on L-threonine to produce (2R, 3R)-epoxy butyric acid; enabling (2R, 3R)-epoxy butyric acid and the intermediate A to undergo a coupling reaction, obtaining an intermediate B, enabling the intermediate B to undergo a cyclization reaction, obtaining an intermediate C, enabling the intermediate C to undergo a hydroxyl protection reaction, obtaining an intermediate D, enabling the intermediate D to be oxidized to form an acetoxy group, and enabling an oxidized product to undergo an ozonation reaction, wherein G is H, F, Cl, Br, a methoxy group, oxethyl or an amino group; and R is H, straight chain alkyl of C1-C6, cyclopropyl, isopropyl, tert-butyl, a phenyl group, p-chlorophenyl, o-chlorophenyl, p-bromophenyl, o-bromophenyl, p-methoxyphenyl, o-methoxyphenyl or m-methoxyphenyl. According to the preparation method, raw materials are cheap and easy to obtain, reaction conditions are mild, the conversion rate and the yield rate are high, and the preparation method is suitable for industrial production.

There is disclosed a method for producing an L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of L-arabinose permease.

The invention discloses a medicine combination which contains 15 amino acids and the preparation method thereof; the medicine combination is characterized in that a compound amino acid injecta with different concentrations are prepared by the 15 amino acids which serve as raw materials and admixture according to the following parts by weight: 6.1-10.8 parts of L-Isoleucine, 8.8-16.6 parts of L-Leucine, 4.6-10.4 parts of L-Lysine Acetate, 0.8-3.0 parts of L-Methionine, 0.8-3.9 parts of L-Phenylalanine, 1.6-5.4 parts of L-threonine, 0.5-1.1 parts of L-Tryptophan, 6.7-10.7 parts of L-Valine, 3.2-9.3 parts of L-alanine, 4.6-7.2 parts of L-arginine, 1.2-2.9 parts of L-Histidine, 5.0-9.6 parts of L-proline, 2.6-6.0 parts of L-serine, 2.6-10.8 parts of glycin, 0.1-1.0 parts of L-Cysteine hydrochloride, 0.1-0.5 parts of citric acid and moderate water for injection. The injecta does not contain sulphite type chemical inhibitor, thoroughly solves the harm of sulphite type on human body, and ensures that the obtained products are safer. The PH value of the injecta is 5.5-7.0. By the accelerated test and quality test, the results show that the stability of the medicine combination which contains 15 amino acids is the same as or better than like products which contains sulphite type sold on market.

An alcohol dehydrogenase (ADH) from hyperthermophilic archaeon Thermococcus guaymasensis was purified to homogeneity and was found to be a homotetramer with a subunit size of 40±1 kDa. The gene encoding the enzyme was cloned and sequenced, and found to have significant sequence homology to known zinc-containing ADHs and L-threonine dehydrogenases with both binding motifs of catalytic zinc and NADP+. The wild-type enzyme is a primary-secondary ADH that exhibits a substrate preference for secondary alcohols and corresponding ketones, and exhibits unusual stereoselectivity. The wild-type enzyme was found to have outstanding thermostability, demonstrating 60% activity after incubation at 80° C. for 40 hours. Site-directed mutagenesis was used to substitute the cyteine residue at position 56 with a serine, to provide the TgADH(C56S) mutant. In the assays that we carried out, we found virtually no difference in enzyme activity and oxygen-sensitivity between the mutant TgADH (C56S) and wild type TgADH.

A process for the production of L-amino acids, in particular L-threonine, in which the following steps are carried out: (a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which the aceA gene or nucleotide sequences coding therefor are attenuated, in particular are switched off, (b) enrichment of the L-amino acid in the medium or in the cells of the bacteria, and (c) isolation of the L-amino acid.

The invention discloses a composition for relieving physical fatigue and enhancing an immunologic function, a preparation method thereof and application thereof. The composite comprises 10 to 100 weight parts of guarana extract, 100 to 800 weight parts of taurine, 1 to 10 weight parts of L-lysine hydrochloride, 1 to 10 weight parts of L-isoleucine, 0.1 to 5 weight parts of L-leucine, 0.2 to 5 weight parts of L-valine, 1 to 15 weight parts of L-aspartate, 0.01 to 0.3 weight part of L-tryptophan, 0.01 to 0.3 weight part of L-phenylalanine, 0.01 to 0.3 weight part of L-threonine, 0.01 to 0.3 weight part of L-methionine, 10 to 100 weight parts of L-alanine, 10 to 100 weight parts of glycine, 1 to 15 weight parts of tea polyphenol and 1 to 20 weight parts of American ginseng extract.

In its several embodiments, this invention discloses a pharmaceutical formulation comprising at least one antineoplastic agent or a pharmaceutically acceptable salt thereof, and at least one dissolution enhancing agent sufficient to substantially dissolve said at least one antineoplastic agent in at least one aqueous diluent, wherein said dissolution enhancing agent is urea, L-histidine, L-threonine, L-asparagine, L-serine, L-glutamine or mixtures thereof; a lyophilized powder comprising said pharmaceutical formulation, and articles of manufacture thereof.

An anti-diarrhea complete formula feed for piglets comprises, by weight, 55-56 parts of extruded corn, 6-10 parts of broken rice, 13-18 parts of enzymatic soybean meal, 9.5-10 parts of low-protein whey powder containing 3% of CP, 3-5 parts of Peruvian steam fishmeal, 0.8-1 part of L-lysine hydrochloride, 0.13-0.18 parts of DL-methionine, 0.09-0.13 parts of L-tryptophan, 0.35-0.4 parts of L-threonine, 0.25-0.3 parts of L-valine, 0.1-0.15 parts of L-isoleucine, 0.18-0.2 parts of L-cystine, 0.08-0.12 parts of choline chloride, 0.83-0.88 parts of calcium hydrogen phosphate, 0.95-1 part of calcium carbonate, 0.8-0.88 parts of salt, 0.04 parts of a vitamin premix, 0.4 parts of a trace elements premix and 0.03-0.05 parts of other auxiliary additives. A feed nutrient ration is reduced, and reasonable burdening is carried out by treating an amount required by an NRC2012 piglet as a standard, so the digestible nutrient content of the feed is improved, thereby the feed resource is reduced, the nutrient draining amount of an animal is reduced, the intestinal digestion burden is reduced, and the diarrhea is mitigated.

The invention relates to a fermentation medium for fermenting and producing glutamic acid from a thermo-sensitive type strain. The components and the final concentration of the fermentation medium in the formula are as follows: 165-205g / L corn starch amylolysis, 15-30g / L maize slurry, 10-18g / L molasses, 0.5-1.5g / L glycine betaine, 5-10g / L bean pulp hydrolysate, 3-8g / L H3PO4, 1.25-1.75g / L MgSO4.7H2O, 0.064-0.084g / L MnSO4.7H2O, 0.055-0.085g / L FeSO4.7H2O, 3-6g / L KCl, 0.08-0.15g / L threonine, 0.8-1.2g / L succinic acid, and 0.1-0.3g / L defoamer, and the solvent is water, the pH value of the fermentation medium is 6.8-7.0 when the phosphoric acid is neutralized by alkali. Due to the appropriate carbon source, the nitrogen source, the carbon nitrogen ratio and the phosphorus content in the medium, the acid production rate is increased, the acid production of the thallus is promoted, the final acid production level is obviously improved by about 5.5% and the glutamic acid yield and the glucose acid conversion ratio are increased.

The invention discloses Chinese herbal medicine complete antimicrobial-free feed special for laying hens in the egg producing period and a preparation method thereof, and belongs to the technical field of animal feed. The defect that antibiotics are included in existing feed is overcome. The feed is prepared from, by weight, 420-580 parts of corn, 40-80 parts of extruded bean pulp, 40-100 parts of bran, 20-50 parts of rice bran, 50-100 parts of maize germ meal, 50-100 parts of DDGS, 50-100 parts of corn gluten meal, 20-40 parts of peanut meal, 5-10 parts of soya-bean oil, 7-9 parts of particle stone powder, 7-9 parts of shell powder, 4-6 parts of calcium hydrophosphate, 3-4 parts of salt, 0.5-1 part of choline chloride, 5-7 parts of L-lysine hydrochloride, 0.5-2 parts of DL-methionine, 0.5-3 parts of L-threonine, 10 parts of compound premix, 1 part of microecologic, 1 part of complex enzyme preparation and 30 parts of Chinese herbal medicine additive. The feed is suitable for being eaten by the laying hens in the egg producing period.