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Method for fermentation production for L-tryptophan by bacteria transforming tdcD enzyme regulation component

A regulatory element and bacterial fermentation technology, applied in the field of amino acid fermentation, can solve the problems such as no transformation of tdcD enzyme regulatory element, no attention to tdcD enzyme encoding gene, etc., and achieve the effect of facilitating popularization and application and improving yield

Active Publication Date: 2013-10-09
NINGXIA EPPEN BIOTECH
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Problems solved by technology

In the current literature reports, the attention through genetic engineering is mainly focused on mtr, aroP, tnaA, trpB, gnd, and traB In terms of isogenes (see Chinese patents 85101270, 93117586, 96111972, 200580043246, 200710056966, 201010598350, etc.), no attention has been paid to the tdcD enzyme and its coding gene for the production of L-tryptophan, let alone the modification of the regulatory elements of the tdcD enzyme , in fact the international patent WO2012135389 discloses the production of oxidation products of aromatic amino acids, which even requires the additional expression of heterologous tdc enzymes

Method used

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Examples

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Embodiment Construction

[0035] The following examples further illustrate the content of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well-known to those skilled in the art and commonly available instruments and reagents on the market. See "Molecular Cloning Experiment Guide (3rd Edition)" (Science Press), Reference to "Microbiology Experiment (4th Edition)" (Higher Education Press) and manufacturer's instructions for corresponding instruments and reagents.

[0036]

[0037] Construction Example Replaced with a promoter with weaker transcriptional activity tdcD Upstream regulatory sequence

[0038] By E. coli tdcD In the upstream sequence analysis, we designed a weak transcription promoter (sequence shown in SEQ ID No: 2) and commissioned the synthesis and construction of the pMD-19T plasmid (available from Dalian Bao Biological Company) by the Institute of Microbiology, Chinese Academy of Sciences to replace the strain tdcD The wild-...

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Abstract

The invention provides a method for fermentation production for L-tryptophan. The method comprises the following steps of: transforming a wild-type regulation component for tdcD genes on a bacterial chromosome so that the expression starting capacity of the regulation component for bacteria obtained by transforming, for the downstream enzyme genes (for example, tdcD genes) of the bacteria is weakened but not disappears; and fermenting by the transformed bacteria to produce L-tryptophan. Additionally, the invention further provides methods and applications derived from the method, and polynucleotides, carriers and bacteria capable of being used in the methods and applications.

Description

technical field [0001] The present invention belongs to the field of amino acid fermentation, in particular, the present invention relates to a method for fermentative production of l-tryptophan and its derivation method and application, as well as polynucleotides, vectors and bacteria that can be used in these methods and applications. Background technique [0002] Production of l-tryptophan by fermentation of l-tryptophan-producing bacteria (eg, Escherichia coli of the genus Escherichia and rod-shaped bacteria of the genus Corynebacterium) has been industrially applied. These bacteria can be bacteria isolated from nature, bacteria obtained through mutagenesis or genetic engineering, or both. In the current literature reports, the attention through genetic engineering is mainly focused on mtr, aroP, tnaA, trpB, gnd, and traB In terms of isogenes (see Chinese patents 85101270, 93117586, 96111972, 200580043246, 200710056966, 201010598350, etc.), no attention has been paid to...

Claims

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Application Information

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IPC IPC(8): C12P13/22C12N15/70C12N1/21C12N15/11C12R1/19
Inventor 马吉银贾慧萍孟刚魏爱英马风勇李小刚杨立鹏
Owner NINGXIA EPPEN BIOTECH
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