A method for efficiently extracting glucosamine from fermentation broth

A technology of glucosamine and fermentation liquid, which is applied in the direction of amino sugar, chemical instruments and methods, sugar derivatives, etc., can solve the problem of low extraction rate of glucosamine, achieve the effects of shortening the saturation adsorption time, improving efficiency, and reducing time

Inactive Publication Date: 2020-01-14
成都本则生科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] For this reason, the technical problem to be solved by the present invention is to provide a method for efficiently extracting glucosamine from fermented liquid to solve the problem of low extraction rate of glucosamine in fermented liquid in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The fermentation broth for extracting the glucosamine in this example is obtained by fermentation according to the method in the prior art, that is, the fermentation broth containing glucosamine and N-acetylglucosamine is obtained by fermentation with Escherichia coli BL21. The culture medium and culture conditions can refer to the culture method recorded in Jiangnan University Liang Fang's "Glucosamine Fermentation Pilot Scale-up and Extraction Technology Research".

[0031] The method for efficiently extracting glucosamine from the fermented liquid described in this embodiment specifically includes the following steps:

[0032] (1) Take glucosamine-containing fermentation broth and add 0.1mol / L hydrochloric acid to deacetylate N-acetylglucosamine; and take the above-mentioned treated fermentation broth for high-speed centrifugation to remove bacteria, collect supernatant, and add silicon Celite (mass ratio is 100:4) to remove impurities such as pigments contained in t...

Embodiment 2

[0038] The fermentation broth for extracting the glucosamine in this example is the same as that in Example 1.

[0039] The method for efficiently extracting glucosamine from the fermented liquid described in this embodiment specifically includes the following steps:

[0040] (1) Take glucosamine-containing fermentation broth and add 0.1mol / L hydrochloric acid to deacetylate N-acetylglucosamine; and take the above-mentioned treated fermentation broth for high-speed centrifugation to remove bacteria, collect supernatant, and add silicon Celite (mass ratio is 100:4) to remove impurities such as pigments contained in the fermentation broth, centrifugal filtration to remove diatomaceous earth to obtain a clarified fermentation broth for subsequent use; after testing, the content of glucosamine in the fermentation broth is 32.7 g / L;

[0041] (2) Get the 10g strongly acidic cation exchange resin 001 * 8 after pretreatment, be placed in 75ml damping fluid and carry out activation eq...

Embodiment 3

[0046] The fermentation broth for extracting the glucosamine in this example is the same as that in Example 1.

[0047] The method for efficiently extracting glucosamine from the fermented liquid described in this embodiment specifically includes the following steps:

[0048] (1) Take glucosamine-containing fermentation broth and add 0.1mol / L hydrochloric acid to deacetylate N-acetylglucosamine; and take the above-mentioned treated fermentation broth for high-speed centrifugation to remove bacteria, collect supernatant, and add silicon Celite (mass ratio is 100:4) to remove impurities such as pigments contained in the fermentation broth, centrifugal filtration to remove diatomaceous earth to obtain a clarified fermentation broth for subsequent use; after testing, the content of glucosamine in the fermentation broth is 32.7 g / L;

[0049] (2) Get the 10g strongly acidic cation exchange resin 001 * 8 after pretreatment, be placed in 75ml damping fluid and carry out activation eq...

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Abstract

The invention belongs to the technical field of microbial fermentation product extraction, and particularly relates to a method for efficiently extracting glucosamine from fermentation broth. On the basis of a method of extracting glucosamine with cation exchange resin in the prior art, a buffer solution of equilibrium activated resin is improved to effectively improve the adsorption efficiency ofthe resin to glucosamine, the saturated adsorption time of the resin is effectively shortened, the time of adsorption treatment is shortened, and the adsorption efficiency is effectively improved. Atthe same time, by improving an eluent in the existing method, glycine can effectively promote the connection of an amino group and the resin and helps to elute the target product, glucosamine, so asto effectively improve the efficiency of desorption process and improve the extraction rate of glucosamine in the fermentation broth on the whole.

Description

technical field [0001] The invention belongs to the technical field of extraction of microbial fermentation products, and in particular relates to a method for efficiently extracting glucosamine from fermentation broth. Background technique [0002] Glucosamine (GlcN), also known as ammonia sugar, glucosamine or glucosamine, is a compound in which one hydroxyl group of glucose is replaced by an amino group. It is an important functional monosaccharide and the first amino group whose structure has been confirmed. Monosaccharide. Glucosamine is easily soluble in water and hydrophilic solvents, and its derivative N-acetylglucosamine (GlcNAc) can be obtained by acid hydrolysis. GlcN exists in almost all organisms, including bacteria, yeast, filamentous fungi, plants and animals, and is the main component of glycoproteins and proteoglycans, as well as chitosan and chitin. [0003] Glucosamine is widely used in the fields of medicine, food and health care. Because glucosamine c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H5/06C07H1/06
CPCC07H1/06C07H5/06
Inventor 吴红光
Owner 成都本则生科技有限公司
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