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Composite type animal intestine regulating preparation and preparation method thereof

A compound, animal-based technology, applied in animal feed, animal feed, bacteria used in food preparation, etc., can solve the problems of preventing animal diseases at the beginning, to enhance intestinal immunity, improve disease resistance, and reduce diarrhea rate and mortality effects

Inactive Publication Date: 2017-11-28
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While the health benefits of lychee polyphenols and glutamine are well accepted by the general public, research in animal disease prevention is still in its infancy

Method used

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  • Composite type animal intestine regulating preparation and preparation method thereof
  • Composite type animal intestine regulating preparation and preparation method thereof
  • Composite type animal intestine regulating preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0028] Implementation Case 1 Preparation of a Compound Animal Intestinal Regulatory Preparation

[0029] 1.1 Material preparation

[0030] (1) strain

[0031] Clostridium butyricum, with the preservation number GIMCC GIM 1.676, and Lactobacillus acidophilus, with the preservation number GIMCC GIM 1.730 were purchased from the Culture Collection Center of Guangdong Institute of Microbiology.

[0032] (2) Lychee polyphenols

[0033] Extracted from lychee shell, the purity is over 90%.

[0034] (3) culture medium

[0035] ①Lactobacillus acidophilus liquid medium formula is: peptone 10.0g, beef extract powder 8.0g, yeast extract powder 4.0g, glucose 20.0g, K 2 HPO 4 2.0g, Na 3 C 6 h 5 o 7 2.0g, CH 3 COONa·3H 2 O 5.0g, MgSO 4 .7H 2 O 0.2g, MnSO 4 4H 2 O0.05g, Tween 80 1ml and distilled water 1L, pH 5.5-6.0, autoclaved at 121°C for 15-20min.

[0036] ②Clostridium enrichment liquid medium: peptone 10.0g, beef extract powder 10.0g, cysteine ​​0.5g, yeast extract powd...

Embodiment example 2

[0046] Implementation Case 2 Application of Compound Probiotics in the Prevention and Treatment of Animal Diarrhea

[0047] 2.1 Diarrhea experiment in mice

[0048] (1) Experimental treatment

[0049] SPF grade Kunming mice were rested for 7 days and randomly divided into 4 experimental treatment groups: model group, Clostridium butyricum group, and Lactobacillus acidophilus conditioning preparation group. The experimental period is 14 days, and the dose is 400mg / kg from the 1st day to the 14th day of the experiment . bw was given the test sample, and as a comparison, the model group was given the same amount of normal saline. On the 10th day of the experiment, the mice were gavaged with castor oil to establish a mouse model of diarrhea, and the indicators of diarrhea were measured. On the 14th day, blood was collected to separate serum, ileum was collected and feces were collected aseptically. Serum was used for the determination of antioxidant indexes (SOD, GSH-Px and CA...

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Abstract

The invention discloses a composite type animal intestine regulating preparation and a preparation method thereof. The composite type animal intestine regulating preparation is mainly compounded from probiotics and prebiotics, wherein the probiotics comprise clostridium butyricum and lactobacillus acidophilus; and the prebiotics comprise lichee polyphenol and glutamine. According to the composite microecological preparation disclosed by the invention, lichee polyphenol and glutamine are utilized to promote clostridium butyricum and lactobacillus acidophilus to proliferate in intestinal tracts, so that the effect of regulating intestinal flora in a two-way mode is brought into play. The composite microecological preparation is relatively good in stability, can regulate intestinal flora of animals, improves disease resistance of the animals, reduces a diarrhea rate and a death rate, can keep a relatively high survival rate, and is suitable for the needs of the feed industry and the livestock breeding industry.

Description

technical field [0001] The invention belongs to the technical field of microbial feed additives, and in particular relates to a compound animal intestine regulating preparation and a preparation method thereof. Background technique [0002] For a long time, the abuse of antibiotics has brought serious threats to animal husbandry and human health. As an emerging feed additive and antibiotic substitute, microecological preparations can directly supplement exogenous beneficial bacteria, regulate the microecological balance of the animal's own intestinal tract, inhibit harmful bacteria, and enhance the body's immunity, thereby promoting the healthy growth of animals And so on. Scholars at home and abroad have done a lot of research on it, and applied it to animal breeding. Many studies and clinical applications have found that probiotics can regulate the balance of animal intestinal microbial flora, prevent diseases, improve feed conversion, improve animal immunity, and imp...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K20/10A23K20/142A23K50/50
CPCA23K10/18A23K20/10A23K20/142A23K50/50A23V2400/113
Inventor 涂杜张军丽徐志宏吕殿红周秀蓉
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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