Natural plant fermentation liquor as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of biological fermentation, and discloses a natural plant fermentation liquid which is prepared from the following raw materials: radix acanthopanacis senticosi, radix angelicae sinensis, fructus rosae laevigatae, phellinus linteus, tea leaves, water, mixed enzyme and a mixed bacterial agent. Wherein the mixed enzyme comprises cellulase, pectinase and tannase, and the mixed microbial agent comprises plant lactobacillus, bifida and lactobacillus acidophilus. The natural plant fermentation liquor is high in safety and good in clarity after the concentration of crude drugs is adjusted, and can be added into cosmetic matrixes. The preparation method is simple, and effective active ingredients of natural plants and tea leaves are extracted to the maximum extent through the synergistic effect of bacteria and enzymes. In addition, the invention also discloses application of the natural plant fermentation liquor in preparation of cosmetics for treating oily sensitive skin.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological fermentation, and particularly relates to a natural plant fermentation broth, a preparation method thereof, and an application thereof. Background Art

[0002] Oily sensitive skin is a special type of skin, which has the characteristics of significantly increased sebum secretion by sebaceous glands and being more sensitive to environmental or physical stimuli. Oily sensitive skin has different manifestations in different areas of the face. The skin in the T-zone of the face, including the forehead, nose, chin and other parts, is prone to oiliness, with a shiny skin surface, larger pores, and is prone to problems such as acne and comedones; the skin in the U-zone of the face, that is, on both sides of the cheeks, often feels dry and sensitive, may show erythema and desquamation, and is accompanied by itching, burning, stinging, dryness or a feeling of tightness. Under physiological or pathological conditions, oily sensitive skin shows a high degree of reactivity. When stimulated physically, chemically, mentally, etc., it is prone to discomfort symptoms such as burning, stinging, itching, and a feeling of tightness, and even objective signs such as erythema, scales, and telangiectasia may appear. The improvement of the skin condition can be achieved by repairing the skin barrier, regulating sebum secretion, and soothing skin inflammation.

[0003] In recent years, the proportion of the population with oily sensitive skin has been continuously rising. In 2022, the incidence rate of oily sensitive skin in the population was as high as 48.5%. Developing new cosmetic raw materials for treating oily sensitive skin has become a current hotspot. China has rich plant resources. With the development of enzyme extraction methods and biological fermentation technologies, by screening and combining natural plants, enzymes and strains to exert the synergistic effect among microorganisms, and then improving the efficiency of extracting effective components from natural plants, it has a positive effect on exploring a natural plant fermentation broth that can be used to treat oily sensitive skin. Summary of the Invention

[0004] The first object of the present invention is to provide a natural plant fermentation broth to solve at least one of the above technical problems.

[0005] The second object of the present invention is to provide a preparation method of a natural plant fermentation broth to solve at least one of the above technical problems.

[0006] The third object of the present invention is to provide an application of the natural plant fermentation broth in the preparation of cosmetics to solve at least one of the above technical problems.

[0007] According to the first aspect of the present invention, there is provided a natural plant fermentation broth, which is prepared from acanthopanax senticosus, angelica acutiloba, rosa laevigata, fomes officinalis, tea leaves, water, a mixed enzyme, and a mixed bacterial agent; wherein, the mixed enzyme includes cellulase, pectinase, and tannase, and the mixed bacterial agent includes lactobacillus plantarum, schizosaccharomyces pombe, and lactobacillus acidophilus.

[0008] In some embodiments, the tea leaves may be selected from at least one of green tea, black tea, white tea, yellow tea, oolong tea, and dark tea.

[0009] Among them, Eleutherococcus senticosus is the dried root, rhizome or stem of the plant Eleutherococcus senticosus (Rupr. et Maxim.) Harms of the Araliaceae family; Angelica acutiloba is the dried root of the plant Angelica acutiloba (Siebet Zucc) Kitag of the Apiaceae family; Rosa laevigata is the dried ripe fruit of the plant Rosa laevigata Michx. of the Rosaceae family; Phellinus linteus is the dried fruiting body of the fungus Phellinus officinalis (Vill. Ex Fr.) Ames. of the Polyporaceae family; green tea is the fresh leaves of the plant Camellia sinensis (L.) O. Kuntze of the Theaceae family that have undergone the fixation process.

[0010] The natural plant fermentation broth of the present invention uses natural plants, Phellinus linteus, tea leaves, a mixed enzyme, and a mixed bacterial agent as raw materials, and has high safety. Among them, Eleutherococcus senticosus and Angelica acutiloba are used as medicine from the rhizome part and contain a lot of cellulose; Rosa laevigata is used as medicine from the fruit; Phellinus linteus is used as medicine from the fruiting body; the tea leaves have a high tannin content. The mixed enzyme composed of cellulase, pectinase, and tannase can destroy the dense structure of the natural plant cell wall, enhance the permeability of barriers such as cell walls and cell interstitium, and promote the diffusion of active ingredients from inside the cells to the extracellular medium. The mixed bacterial agent composed of Lactobacillus plantarum, Schizosaccharomyces pombe, and Lactobacillus acidophilus degrades the macromolecular active ingredients of natural plants such as Eleutherococcus senticosus, Angelica acutiloba, and Rosa laevigata, Phellinus linteus, and tea leaves into small molecular active ingredients, greatly increasing the absorption rate of the active ingredients in natural plants, Phellinus linteus, and tea leaves by the human body. The two perform fungal and enzymatic co-fermentation on natural plants, Phellinus linteus, and tea leaves, and extract the effective active ingredients from natural plants to the greatest extent, thereby improving the medicinal efficacy of the natural plant fermentation broth.

[0011] In some embodiments, by mass, Eleutherococcus senticosus is 1 - 5 parts, Angelica acutiloba is 1 - 5 parts, Rosa laevigata is 1 - 5 parts, Phellinus linteus is 1 - 5 parts, tea leaves are 1 - 5 parts, and water is 6 - 8 parts.

[0012] In some embodiments, by mass, Eleutherococcus senticosus is 1 part, Angelica acutiloba is 1 part, Rosa laevigata is 1 part, Phellinus linteus is 1 part, tea leaves are 1 part, and water is 6 parts.

[0013] In some embodiments, the mass of the mixed enzyme can be 0.3% - 0.5% of the total mass of Eleutherococcus senticosus, Angelica acutiloba, Rosa laevigata, Phellinus linteus, tea leaves, and water; among them, the mass ratio of cellulase, pectinase, and tannase in the mixed enzyme can be 1:1:2.

[0014] In some other embodiments, the mass of the mixed enzyme can be 0.4% of the total mass of Eleutherococcus senticosus, Angelica acutiloba, Rosa laevigata, Phellinus linteus, tea leaves and water.

[0015] In some embodiments, the dosage of the mixed microbial agent can be 0.5% - 0.7% of the total mass of Eleutherococcus senticosus, Angelica acutiloba, Rosa laevigata, Phellinus linteus, tea leaves and water; preferably, the dosage of the mixed microbial agent can be 0.5% of the total mass of Eleutherococcus senticosus, Angelica acutiloba, Rosa laevigata, Phellinus linteus, tea leaves and water.

[0016] In some embodiments, the mass ratio of Lactiplantibacillus plantarum, Saccharomyces boulardii and Lactobacillus acidophilus can be 1:1:1; Lactiplantibacillus plantarum can be Lactiplantibacillus plantarum Lp - G18, Saccharomyces boulardii can be Saccharomyces boulardii BB536, and Lactobacillus acidophilus can be Lactobacillus acidophilus LA - G80.

[0017] In some embodiments, the raw materials of the natural plant fermentation broth further include sucrose and rice flour. Specifically, sucrose and rice flour provide energy for Lactiplantibacillus plantarum, Saccharomyces boulardii and Lactobacillus acidophilus.

[0018] In some embodiments, the dosage of sucrose can be 7% - 8% of the total mass of Eleutherococcus senticosus, Angelica acutiloba, Rosa laevigata, Phellinus linteus, tea leaves and water, and the dosage of rice flour can be 3% - 5% of the total mass of Eleutherococcus senticosus, Angelica acutiloba, Rosa laevigata, Phellinus linteus, tea leaves and water.

[0019] In some embodiments, the dosage of sucrose can be 7.5% of the total mass of Eleutherococcus senticosus, Angelica acutiloba, Rosa laevigata, Phellinus linteus, tea leaves and water, and the dosage of rice flour can be 3% of the total mass of Eleutherococcus senticosus, Angelica acutiloba, Rosa laevigata, Phellinus linteus, tea leaves and water.

[0020] According to the second aspect of the present invention, there is provided a method for preparing a natural plant fermentation broth, comprising the following steps:

[0021] (1) Mix and grind Eleutherococcus senticosus, Angelica acutiloba, Rosa laevigata, Phellinus linteus, tea leaves with water to obtain a slurry;

[0022] (2) Add the mixed enzyme to the slurry and carry out enzymatic hydrolysis at 50 - 60 °C to obtain an enzymatic hydrolysate;

[0023] (3) Dissolve sucrose and rice flour in the enzymatic hydrolysate to obtain a mixed solution;

[0024] (4) Filter the mixed solution and seal the filtrate, and carry out high - pressure steam sterilization on the sealed filtrate and then cool it;

[0025] (5) Activate Lactobacillus plantarum, Saccharomyces boulardii, and Lactobacillus acidophilus, and mix the activated Lactobacillus plantarum, Saccharomyces boulardii, and Lactobacillus acidophilus to obtain a mixed microbial agent;

[0026] (6) Inoculate the mixed microbial agent into the cooled filtrate;

[0027] (7) Ferment the inoculated filtrate at 35 - 50 °C for 6 - 8 days to obtain the product.

[0028] The preparation method of the natural plant fermentation broth of the present invention first preliminarily destroys the cell wall of natural plants by grinding, and then adds a mixed enzyme for enzymatic hydrolysis reaction to further destroy the dense structure of the plant cell wall, promoting the diffusion of macromolecular active ingredients of natural plants such as Acanthopanax senticosus, Angelica acutiloba, Rosa laevigata, medicinal Phellinus, and tea from inside the cells into the enzymatic hydrolysate. Then, nutrients such as sucrose and rice flour are added to provide nutrition for the mixed microbial agent. Finally, the mixed microbial agent is used for fermentation to degrade the macromolecular active ingredients into small molecular active ingredients. The preparation method is simple, which not only improves the extraction efficiency of the active ingredients of natural plants, medicinal Phellinus, and tea, but also increases the absorption rate of the human body to the active ingredients of natural plants, thereby enhancing the medicinal efficacy of the natural plant fermentation broth.

[0029] In some embodiments, in step (2), the treatment time of enzymatic hydrolysis can be 3 - 5 h.

[0030] In some embodiments, in step (4), the filtration method can be to filter the mixed solution using a clean gauze.

[0031] In some embodiments, in step (4), the method of sealing the filtrate can be to dispense the filtrate into 50 mL conical flasks, and then seal the conical flask mouths with a sterile culture container sealing film. Specifically, it can effectively prevent external miscellaneous bacteria from contaminating the filtrate.

[0032] In some embodiments, in step (4), the method of high-pressure steam sterilization can be to sterilize at a temperature of 100 - 110 °C and a pressure of 102 - 105 kPa for 10 - 15 min.

[0033] In some embodiments, in step (5), the activation method can be to take out the cryopreservation tubes containing Lactobacillus plantarum, Saccharomyces boulardii, and Lactobacillus acidophilus from the low-temperature environment, and then gently shake the cryopreservation tubes in a water bath environment at 36 - 37 °C until the ice in the cryopreservation tubes completely melts.

[0034] According to the third aspect of the present invention, there is provided the use of the natural plant fermentation broth in the preparation of cosmetics. Among them, the cosmetics are cosmetics having at least one of the following functions:

[0035] (1) Improve the skin's water-locking function;

[0036] (2) Improve the skin barrier function;

[0037] (3) Improve skin roughness and gloss;

[0038] (4) Antihistamine and anti-allergy soothing effects;

[0039] (5) Moisturize and control oil.

[0040] The microbial indexes of the natural plant fermentation broth of the present invention comply with the hygiene standards of cosmetics, have high safety, and the natural plant fermentation broth after adjusting the crude drug concentration has a light color and high clarity, and is suitable for adding to a cosmetic matrix to prepare cosmetics.

[0041] In some embodiments, the application can be the application of the natural plant fermentation broth in the preparation of cosmetics for treating oily and sensitive skin. Specifically, the natural plant fermentation broth is added as a raw material to the cosmetics, and the cosmetics are safe and non-irritating, and can exert the characteristics of easy absorption and drug efficacy of the natural plant fermentation broth.

[0042] According to the fourth aspect of the present invention, there is provided a cosmetic containing the above natural plant fermentation broth, which is also a cosmetic having at least one of the following functions:

[0043] (1) Improve the skin water-locking function;

[0044] (2) Improve the skin barrier function;

[0045] (3) Improve skin roughness and gloss;

[0046] (4) Antihistamine and anti-allergy soothing effects;

[0047] (5) Moisturize and control oil.

[0048] In some embodiments, the cosmetic can be essence, lotion, cream, mask, aqueous solution. Specifically, the essence containing the natural plant fermentation broth prepared from the natural plant fermentation broth as a raw material is non-irritating. Tests show that the essence not only has good antihistamine and anti-allergy skin soothing effects, but also has a significant moisturizing and oil control effect on the skin, and shows good tolerance in the treatment of combination and oily skin.

[0049] The technical effects of the present invention are:

[0050] (1) The natural plant fermentation broth of the present invention uses acanthopanax senticosus, angelica acutiloba, rosa laevigata, fomes officinalis, tea leaves, mixed enzymes and mixed bactericides as raw materials, has high effective active ingredients, and has good drug efficacy;

[0051] (2) The preparation method of the natural plant fermentation broth of the present invention promotes the diffusion of the macromolecular active ingredients of natural plants, Phellinus linteus, and tea from inside the cells to outside the cells by successively adding a mixed enzyme and a mixed bacterial agent, and degrades the macromolecular active ingredients into small molecular active ingredients. This not only improves the extraction efficiency of the active ingredients in natural plants, Phellinus linteus, and tea, but also increases the absorption rate of the active ingredients of natural plants by the human body, thereby enhancing the medicinal efficacy of the natural plant fermentation broth.

[0052] (3) After adjusting the crude drug concentration, the natural plant fermentation broth of the present invention has high clarity and is suitable for adding to a cosmetic matrix to prepare cosmetics. The prepared cosmetics are safe and non-irritating. The essence prepared from the natural plant fermentation broth has a significant moisturizing and oil-control effect on facial skin and has a good soothing effect on antihistamine and anti-allergy skin. Description of the Drawings

[0053] Figure 1 Pictures of the products of Example 1, Comparative Example 1, and Comparative Example 2 after adjusting the crude drug concentration. Figure 1 (A) Picture of the natural plant extract of Comparative Example 1 after adjusting the crude drug concentration. Figure 1 (B) Picture of the natural plant fermentation broth of Example 1 after adjusting the crude drug concentration. Figure 1 (C) Picture of the natural plant extract of Comparative Example 2 after adjusting the crude drug concentration.

[0054] Figure 2 Magnified pictures of the skin of the subjects before and after using the essence containing the natural plant fermentation broth in Test Example 2. Figure 2 (A) Magnified picture of the skin of the subject before antihistamine treatment with the essence containing the natural plant fermentation broth. Figure 2 (B) Magnified picture of the skin of the subject after antihistamine treatment with the essence containing the natural plant fermentation broth.

[0055] Figure 3 Pictures of the changes in the facial skin of the subjects before and after treatment in Test Example 3. Figure 3 (A) Picture of the skin of the subject before treatment with the essence containing the natural plant fermentation broth. Figure 3 (B) Picture of the skin of the subject 14 days after treatment with the essence containing the natural plant fermentation broth. Detailed Embodiments

[0056] The present invention will be further described in detail below with reference to the accompanying drawings, but the embodiments of the present invention are not limited thereto. The raw materials and reagents involved in the following examples can be obtained from commercial channels.

[0057] The acanthopanax senticosus, angelica acutiloba, and rosa laevigata in the present invention were purchased from Kangmei Pharmaceutical Company; the fomes officinalis was purchased from the Qingping Chinese Medicinal Materials Professional Market in Guangzhou; the green tea was purchased from Guangshan Hanshi Tea Co., Ltd. in Henan Province. The above medicinal materials were all identified as genuine products by Chief Pharmacist Qiu Zhenwen of the First Affiliated Hospital of Guangzhou University of Chinese Medicine and complied with the standards of the Chinese Pharmacopoeia or the Xinjiang Local Pharmacopoeia. The Lactiplantibacillus plantarum Lp-G18, Saccharomyces boulardii BB536, and Lactobacillus acidophilus LA-G80 were all purchased from the China Center for Type Culture Collection; the cellulase (200 U / mg), pectinase (100 U / mg), and tannase (100 U / mg) were purchased from Shandong Longkete Enzyme Preparation Co., Ltd.

[0058] It should be noted that green tea is only one implementation mode of tea varieties. Tea varieties can also be selected as black tea, white tea, yellow tea, oolong tea, or dark tea according to needs.

[0059] The homogenizing and emulsifying equipment used for emulsification in the present invention is the FV-30L FISCO vacuum homogenizing emulsifier produced by Shanghai Fluke Fluid Machinery Manufacturing Co., Ltd., which has functions such as homogenization, stirring, and temperature control. The homogenizing and emulsifying equipment produced by other manufacturers for the preparation of cosmetics can also be used in the present invention, as long as it is operated strictly according to the process parameters described in the present invention, the effects described in the present invention can be achieved.

[0060] Example 1

[0061] This example provides a preparation method for a natural plant fermentation broth, including the following steps:

[0062] (1) Weigh 50 grams each of acanthopanax senticosus, angelica acutiloba, rosa laevigata, fomes officinalis, and green tea respectively, crush them, add 300 grams of water and mix, then grind to obtain a slurry.

[0063] (2) Mix cellulase, pectinase, and tannase in a mass ratio of 1:1:2 to obtain a mixed enzyme. Under the condition of 55 °C, add the mixed enzyme with a dosage of 0.4% of the total mass of the slurry to the slurry, and enzymatically hydrolyze for 4 hours to obtain an enzymolysis solution.

[0064] (3) Add sucrose with a dosage of 7.5% of the total mass of the slurry and rice flour with a dosage of 3% of the total mass of the slurry to the enzymolysis solution, and mix evenly until the sucrose and rice flour are completely dissolved in the enzymolysis solution to obtain a mixed solution.

[0065] (4) Filter the mixed solution with a clean gauze, then sub-pack the filtrate obtained after filtration into 50 mL conical flasks, and seal the bottle mouths with a sterile culture container sealing film; then sterilize the conical flasks at 105 °C and a pressure of 103.4 kPa for 10 minutes, cool to room temperature, and then transfer the conical flasks to a laminar flow hood.

[0066] (5) Take out the cryopreservation tubes containing Lactobacillus plantarum Lp-G18, Saccharomyces boulardii BB536, and Lactobacillus acidophilus LA-G80 from the cryopreservation environment, place them in 37°C, and gently shake the cryopreservation tubes continuously until the ice is completely dissolved. Then, use a sterile micropipette to respectively aspirate the activated Lactobacillus plantarum Lp-G18 bacterial liquid, Saccharomyces boulardii BB536 bacterial liquid, and Lactobacillus acidophilus LA-G80 bacterial liquid from the cryopreservation tubes, and mix the Lactobacillus plantarum Lp-G18 bacterial liquid, Saccharomyces boulardii BB536 bacterial liquid, and Lactobacillus acidophilus LA-G80 bacterial liquid in a mass ratio of 1:1:1 to obtain a mixed bacterial agent;

[0067] (6) Inoculate the mixed bacterial agent into the mixed liquid in the conical flask, and the dosage of the mixed bacterial agent is 0.5% of the total mass of the slurry;

[0068] (7) Place the conical flask into a constant temperature and humidity incubator, and carry out airtight fermentation at a temperature of 40°C for 7 days to obtain a natural plant fermentation broth.

[0069] Comparative Example 1

[0070] This comparative example provides a natural plant extract, and its preparation method includes the following steps:

[0071] Weigh 50 grams each of Eleutherococcus senticosus, Angelica acutiloba, Rosa laevigata, Phellinus linteus, and green tea respectively, crush them, then add 300 grams of 70% ethanol, heat to 100°C and decoct for 30 minutes, and extract the filtrate once to obtain it.

[0072] Comparative Example 2

[0073] This comparative example provides a natural plant extract, and its preparation method includes the following steps:

[0074] Weigh 50 grams each of Eleutherococcus senticosus, Angelica acutiloba, Rosa laevigata, Phellinus linteus, and green tea respectively, crush them, then add 300 grams of water, heat to 100°C and decoct for 30 minutes, and extract the filtrate once to obtain it.

[0075] Adjust the natural plant fermentation broth prepared in Example 1, the natural plant extract in Comparative Example 1, and the natural plant extract in Comparative Example 2 to a crude drug concentration of 0.1 g of crude drug / mL, and then compare the colors and clarities of the three. The results are as Figure 1 shown, where Figure 1 (A) is a picture of the natural plant extract in Comparative Example 1 after adjusting the crude drug concentration, Figure 1 (B) is a picture of the natural plant fermentation broth in Example 1 after adjusting the crude drug concentration, Figure 1(C) is a picture of the natural plant extract of Comparative Example 2 after adjusting the crude drug concentration. It can be observed that after adjusting the crude drug concentration, the natural plant fermentation broth of Example 1 has the lightest color and good clarity, and is suitable for adding to the cosmetic matrix.

[0076] Application Example 1

[0077] This application example provides the application of the natural plant fermentation broth in the preparation of essence, and the formula composition of the essence containing the natural plant fermentation broth is shown in Table 1.

[0078] Table 1 Formula table of essence containing natural plant fermentation broth

[0079] Raw material name Dosage added (%) Manufacturer Natural plant fermentation broth 70 Prepared by the preparation method of Example 1 Water The balance 1,2-Pentanediol 5 Yuanli Chemical Group Butanediol 3 Showa Denko K.K. (Japan) Low molecular weight hyaluronic acid 0.05 Bloomage Biotechnology Co., Ltd. Allantoin 0.1 Samp Biochemical Co., Ltd. Lubrajel 1 FTC Co., Ltd. (South Korea) Ethylhexylglycerin 0.1 Suzhou Huaxinxu Chemical Technology Co., Ltd. p-Hydroxyacetophenone 0.5 Shaanxi Xiazhou Biotechnology Co., Ltd. Xanthan gum 0.5 Meihua Biotechnology Co., Ltd. Polyhexamethylene biguanide 0.4 Lonza Group

[0080] The production process of this essence includes the following steps:

[0081] According to the formula table in Table 1, wet the low-molecular-weight hyaluronic acid and xanthan gum with water first and fully swell them for 12 h, then add the swollen low-molecular-weight hyaluronic acid and xanthan gum into water and stir to disperse evenly to obtain a mixture, and then add other raw materials in Table 1 except polyhexamethylene biguanide and p-hydroxyacetophenone to the mixture in turn, heat up to 85 °C, stir at a speed of 500 r / min for 30 minutes, then cool down to 40 °C, add polyhexamethylene biguanide and p-hydroxyacetophenone, and continue to emulsify at a speed of 500 r / min for 15 minutes to obtain the essence containing the natural plant fermentation broth.

[0082] It should be noted that the selection of the raw materials and addition amounts used in Table 1 is the most preferred for exerting the efficacy of the natural plant fermentation broth of the present invention, but the selection of the cosmetic matrix of the essence containing the natural plant fermentation broth of the present invention is not limited to this. The common cosmetic matrices that can be applied to cosmetics produced by other manufacturers can also be used as the formula raw materials of the essence containing the natural plant fermentation broth, and the dosage only needs to meet the national cosmetic additive dosage standard. It should also be noted that by exerting its own drug efficacy through the natural plant fermentation broth, the corresponding skin care effect can be achieved in cosmetics. The preparation of essence is only one of the implementation methods. By selecting the cosmetic matrix and adjusting the dosage, the natural plant fermentation broth can also be used as a raw material to prepare cosmetics such as emulsion, cream, mask, and aqueous solution.

[0083] Test Example 1

[0084] This test example detects the number of microorganisms in the natural plant fermentation broth of Example 1, and the results are shown in Table 2.

[0085] Table 2 Microbial indicators of natural plant fermentation broth

[0086] Microorganism Index Coliform group <100 (MPN / 100g) Mold <50 (CFU / g) Yeast <50 (CFU / g) Pathogenic bacteria (Salmonella, Staphylococcus aureus, Shigella) Not detected

[0087] As can be seen from Table 2, in the natural plant fermentation broth prepared in Example 1, the most probable number (MPN) of microorganisms per 100 grams of the sample for coliform group is less than 100, the total mold count is less than 50 CFU / g, the total yeast count is less than 50 CFU / g, and no pathogenic bacteria are detected. This indicates that the microbial indicators of the natural plant fermentation broth meet the hygiene standards for cosmetics, are suitable for use on the skin, and have high safety.

[0088] Test Example 2

[0089] In this test example, the essence containing the natural plant fermentation broth of Application Example 1 was used for a clinical trial to study the effect of the essence containing the natural plant fermentation broth of the present invention on moisturizing and repairing facial skin.

[0090] 1.1 Inclusion criteria for subjects

[0091] (1) Healthy female subjects aged 20 - 55 years, with dry skin, water content less than 60, positive in the lactic acid sting test screening, and no definite systemic organic diseases; (2) The subjects are willing not to use other skin care products, cosmetics and receive other facial treatments (such as: drugs, chemical peeling, laser, etc.) during the whole research period; (3) Comply with the test requirements and complete the whole test during the whole research period, and be able to complete the follow-up as required; (4) Have signed the informed consent form and are willing to complete the process of this research.

[0092] 1.2 Exclusion criteria for subjects

[0093] (1) Those who are allergic to cosmetic preparations and components or have a history of severe skin allergies; (2) Those who cannot visit the doctor on time or apply other hormone-containing drugs externally by themselves during the treatment period; (3) Pregnant or lactating women.

[0094] 1.3 General information of subjects

[0095] Deputy Chief Physician Xiao Wei of Guangzhou Zihetang Xinghe Traditional Chinese Medicine Clinic was entrusted to recruit 82 healthy female subjects who met the requirements of this study, with an average age of (36.15 ± 7.23) years.

[0096] 1.4 Test method

[0097] Referring to the method described in the literature (Lei Cuiting, Zheng Muchuang, Chen Chuwei. Discussion on the efficacy evaluation method of skin repair cosmetics [J]. Detergent & Cosmetics, 2021, 44(12): 28 - 30), this clinical trial adopted a self - before - and - after control. The right cheek of the subject was selected as the test area. After cleaning the skin, it was applied in a circular motion, avoiding easily allergic parts such as the corners of the eyes, and gently massaged until the remaining essence was completely absorbed, once in the morning, once at noon, and once in the evening, 5 g each time, for 14 consecutive days, a total of 1 treatment course. During this period, no other similar products were allowed to be replaced or used in combination.

[0098] Precautions: During the trial, it is recommended that the subjects change their lifestyle and behavior, such as ensuring sufficient sleep, quitting smoking and alcohol, avoiding spicy, raw, cold and greasy foods, preventing dust irritation and strenuous exercise, and protecting from sun exposure.

[0099] 1.5 Observation indicators

[0100] 1.5.1 Detection of skin physiological function indicators

[0101] Before treatment, 14 days after treatment, and 28 days after treatment, the transepidermal water loss (TEWL) and the water content of the stratum corneum of the facial skin of the subjects were measured using the MPA9 multi-functional skin tester of Courage Khazaka, Germany, and the skin pH value was measured using the Skin-pH meter pH905 tester of Courage Khazaka, Germany. The test environment was a dedicated skin function test room with no ventilation, no direct sunlight, a room temperature of 22 - 24°C, and a relative humidity of 45% - 55%. The specific method was that on the test day, the subjects did not use any facial topical medications or cosmetics, cleaned their faces routinely, and after resting in the skin function test room for 30 minutes to adapt to the environment, the tests were conducted. According to the instrument operation regulations, each of the 3 indicators of the same detection site of the subjects was tested 3 times, with an interval of about 5 minutes in between, and the average value was taken as the result of this determination.

[0102] 1.5.2 Lactic acid sting value score

[0103] The subjects cleaned their faces and dried them with lint-free paper. After resting in the skin function test room for 30 minutes to adapt to the environment, the test was conducted. 50 μL of 10% lactic acid aqueous solution was applied to either side of the nasolabial groove on the cheek, and the subjects evaluated the discomfort degrees of itching, stinging, and burning pain at the test site at 30 s, 2.5 min, and 5 min respectively. The scoring was based on a 4-point scale (0 points: no sensation, 1 point: mild, 2 points: moderate, 3 points: severe). If the cumulative score of the stinging sensation at 30 s, 2.5 min, and 5 min was greater than or equal to 3 points, it was considered positive for lactic acid sting.

[0104] 1.5.3 Human patch test

[0105] A total of 30 female volunteers meeting the subject inclusion criteria were selected, with an average age of (30.56 ± 5.63) years. Qualified patch test equipment was selected. Using the closed patch test method, approximately 0.02 - 0.025 mL of the test substance was placed in the patch test device, and the patch test device was applied to the back of the subjects with a low-allergy tape. After 24 h, the test substance was removed, and the skin reactions were observed at 0.5 / 24 / 48 h after removal. The results were recorded according to the skin reaction grading standard in the "Technical Specifications for Cosmetics Safety (2015 Edition)".

[0106] 1.5.4 Self - satisfaction evaluation of the subjects

[0107] The subjects evaluated their self - satisfaction with the overall improvement of skin indicators such as skin dryness and skin gloss according to their own skin conditions. Compared with the baseline (skin condition before treatment), the self - satisfaction scores included very satisfied (improvement degree ≥ 90%), satisfied (60% ≤ improvement degree < 90%), average (20% ≤ improvement degree < 60%), and dissatisfied (improvement degree < 20%). The satisfaction rate = (number of very satisfied + satisfied cases) / total number of cases × 100%.

[0108] 1.6 Adverse reactions

[0109] By asking questions or during follow - up visits, observe whether there are any adverse reaction symptoms and signs such as facial allergies in the subjects before and after treatment.

[0110] 1.7 Statistical methods

[0111] The data was entered into SPSS 25.0 software after double - checking by two people and statistical analysis was carried out. Measurement data were expressed as mean ± standard deviation and independent - sample t - test was used for pairwise comparison between groups, and paired t - test was used for within - group comparison. The clinical efficacy evaluation was rank data, expressed as frequency and frequency, and rank - sum test was used for analysis. When P < 0.05, the difference was considered statistically significant.

[0112] 2 Results

[0113] 2.1 Comparison of skin physiological function detection indexes of the subjects before and after treatment

[0114] Table 3 shows the skin physiological function detection indexes of the subjects before and after treatment. The data shows that compared with before treatment, the water content of the stratum corneum of the subjects increased significantly 14 days after treatment, and the TEWL decreased significantly, with statistical difference (P < 0.01), and the pH value had no obvious change. 28 days after treatment, the water content of the stratum corneum of the subjects increased significantly, and the TEWL decreased significantly, with statistical difference (P < 0.01), and the pH value had no obvious change.

[0115] Table 3 Skin physiological function detection indexes of the subjects before and after treatment

[0116]

[0117] 2.2 Lactic acid stinging score

[0118] The skin lactic acid stinging score values of the subjects before and after treatment are shown in Table 4. It can be seen that compared with before treatment, the lactic acid stinging scores of the subjects 14 days and 28 days after treatment decreased significantly, with statistical difference (P < 0.01).

[0119] Table 4 Skin Lactic Acid Prickling Score Values of Subjects before and after Treatment

[0120]

[0121] 2.3 Human Patch Test Results

[0122] Table 5 shows the human skin patch test results of the essence containing natural plant fermentation broth. The results show that none of the 82 subjects had symptoms such as faint erythema, erythema, edematous erythema, or significant edema, indicating that under certain environments, usage methods, and dosages, Hengmeifu Balancing Moisturizing Lotion does not affect the barrier function of the human body and has no obvious irritation to human skin.

[0123] Summary of Human Skin Patch Test Results in Table 5

[0124]

[0125] 2.4 Comparison of Self-Satisfaction Evaluations before and after Treatment

[0126] Table 6 shows the comparison of self-satisfaction evaluations of subjects before and after treatment. The questionnaire survey results show that the subjects think the product has a good texture, is easy to apply, absorbs well, the skin is not sticky after use, is moisturizing, and the dryness and tightness are relieved, and the phenomena of skin dehydration, fine lines, skin sensitivity, and flushing have also improved. As shown in Table 3, the satisfaction of the subjects before treatment was 2.44%, the satisfaction after 14 days of treatment was 58.33%, and the satisfaction after 28 days of treatment was 86.59%, with a statistically significant difference (P < 0.01). 82 subjects were satisfied or relatively satisfied with both their suitable skin types and the overall satisfaction of the product, and within the acceptable price range, they were all willing to purchase the product.

[0127] Table 6 Comparison of Self-Satisfaction Evaluations of Subjects before and after Treatment N(p / %)

[0128]

[0129] 2.5 Adverse Reactions

[0130] After 28 days of treatment and observation, no one had adverse reactions, and all the enrolled subjects completed the treatment of their respective course of treatment. The compliance was good during the entire research period, and there was no dropout during the research process.

[0131] 3 Conclusions

[0132] The results of this test example show that the essence containing natural plant fermentation broth of the present invention can significantly improve the water-locking and barrier functions of the skin, effectively improve the roughness and glossiness of the facial skin of the subjects. The results of the skin lactic acid stinging score show that after the subjects used the essence containing natural plant fermentation broth of the present invention for 28 days, their skin's ability to respond to external stimuli was significantly enhanced, and the skin tolerance increased by 4.09 times. The results of the human patch test show that the formula of the essence containing natural plant fermentation broth of the present invention is safe and non-irritating or slightly irritating to human skin.

[0133] Test Example 3

[0134] In this test example, the essence containing natural plant fermentation broth of Application Example 1 was used for a histamine experiment to study the anti-histamine soothing effect of the essence containing natural plant fermentation broth of the present invention.

[0135] 1.1 Test method

[0136] Referring to the method described in the literature (Cai Yiwen, Liu Yuan, Song Chuang, etc. Research progress of anti-allergic safety evaluation models for cosmetics [J]. Daily Chemicals Science, 2021, 44(3): 48-54), histamine was applied to the skin to simulate an acute skin allergic reaction, and then the essence containing natural plant fermentation broth of Application Example 1 was applied to the skin experimental area to test the soothing effect of the essence containing natural plant fermentation broth of the present invention on allergic skin.

[0137] 1.2 Test conditions and test steps

[0138] 1.2.1 Test conditions

[0139] Test instrument: German CK skin tester Cutometer dual MPA580; Probe used: Skin red pigment test probe; Test environmental conditions: Indoors, without strong sunlight or lights; Test environmental temperature is 20-25°C, humidity is 40%-60%, and real-time dynamic monitoring is carried out.

[0140] 1.2.2 Test steps

[0141] The subjects washed the inner elbows with clean water, dried them with paper towels, and marked two 2×2 cm areas on the inner sides of the left and right elbows during the experiment. 2Test area: Take a photo and test the red pigment with a CK instrument, and record it in the red pigment record form for the antihistamine experiment; Drop 10 μL of 5% histamine solution on the marked skin surface, massage and rub to absorb it, and record the time of applying histamine; Record the peak time of redness, swelling and itching within 15 minutes, take a photo and test the red pigment with a CK instrument, and record it in the red pigment record form for the antihistamine experiment; Apply the essence containing natural plant fermentation broth of the present invention on one of the test skins, and apply deionized water on the other test skin as a blank control, and record the time of applying the sample; Test the change value of the red pigment with a CK instrument at 15 min, 30 min, 60 min, and 90 min after applying the test product, and record it in the red pigment record form for the antihistamine experiment; The subject feels the itching relieved starting from dropping the test product, and record the time, observe the red halo, and the regression area of the papules is greater than 1 / 2, and record the time, and record it in the test record form for the antihistamine soothing effect.

[0142] 2 Results

[0143] The skin of the subject before and after using the essence containing natural plant fermentation broth is as Figure 2 shown, Figure 2 (A) is a magnified picture of the subject's skin before antihistamine using the essence containing natural plant fermentation broth, Figure 2 (B) is a magnified picture of the subject's skin after antihistamine using the essence containing natural plant fermentation broth. It can be observed that the subject's skin had obvious redness and swelling symptoms before using the essence containing natural plant fermentation broth, and the skin condition of the subject had an obvious fading effect after use. The antihistamine soothing effect is shown in Table 7.

[0144] Table 7 Antihistamine Soothing Effect of Subjects

[0145] Itching relief time (min) Swelling reduction time (min) Redness fading time (min) 8.22±1.43 15.11±4.02 23.10±5.22

[0146] 3 Conclusions

[0147] The results of this test example show that the essence containing natural plant fermentation broth has an obvious effect of reducing swelling and redness on the skin. Compared with the blank area, the red pigment value in the skin test area where the subject applied the essence containing natural plant fermentation broth shows a more obvious downward trend. After the subject externally applied the essence containing natural plant fermentation broth of the present invention, the itching sensation can be significantly relieved in about 8 minutes, the swelling can be significantly reduced in 15 minutes, and the redness begins to fade after 23 minutes. In summary, the essence containing natural plant fermentation broth of the present invention has a good soothing effect on antihistamine and anti-allergy skin.

[0148] Test Example 4

[0149] In this test example, the essence containing natural plant fermentation broth of Application Example 1 was used for a clinical trial on facial skin to study the clinical efficacy of the essence containing natural plant fermentation broth of the present invention in moisturizing and controlling oil on facial skin.

[0150] 1.1 Subject inclusion criteria

[0151] (1) Healthy subjects aged 18 - 40 years old with oily facial skin texture; (2) Subjects who can cooperate well with the experimenters and maintain a regular lifestyle during the study; (3) Subjects who can read and understand all the contents of the informed consent form and voluntarily sign the informed consent form; (4) During the experiment, subjects are required to stop using skin care products, no longer participate in clinical trials at any other research centers, and agree not to use any cosmetics, drugs, and health products that may affect the results; (5) When testing the oil control effect, it is required that the subjects have combination or oily skin on the face and the initial amount of sebum on the forehead on the day of inclusion in the test is greater than 150 μg / cm 2 。

[0152] 1.2 Subject exclusion criteria

[0153] (1) Subjects with a history of severe skin allergies; (2) Subjects with infectious or inflammatory diseases on the face; (3) Subjects who have received any facial beauty treatments in the past six months; (4) Pregnant or lactating women.

[0154] 1.3 General information of subjects

[0155] 40 volunteers diagnosed and treated by Deputy Chief Physician Xiao Wei of Guangzhou Zihetang Xinghe Traditional Chinese Medicine Clinic at Guangzhou Zihetang Xinghe Traditional Chinese Medicine Clinic, aged 20.25 - 30.72 (23.52 ± 4.61) years old. Before the start of the experiment, all volunteers signed the informed consent form.

[0156] 1.4 Test methods

[0157] Referring to the method described in the reference (China Industry - University - Research Cooperation Promotion Association. Test methods for seven functions of cosmetics: anti - wrinkle, firming, moisturizing, oil control, repair, nourishment, and soothing [J]. Group standard of China Industry - University - Research Cooperation Promotion Association, T / CAB 0152 - 2022, issued on June 24, 2022), this clinical trial adopts a self - controlled before - and - after design. After cleaning the skin every day, the subjects apply the essence containing natural plant fermentation broth of the present invention on the right face, 3 mL each time, 2 times a day, for 14 consecutive days, with a total of 28 applications. During this period, other similar products are not allowed to be replaced or used in combination.

[0158] Precautions: During the experiment, the researchers recommend that the subjects change their lifestyle and behavior patterns, such as ensuring sufficient sleep, quitting smoking and drinking, avoiding spicy, raw, cold, and greasy foods, avoiding dust irritation and strenuous exercise, and preventing sun exposure.

[0159] 1.5 Detection of skin barrier function indicators of subjects

[0160] The researchers measured the transepidermal water loss (TEWL), water content of the stratum corneum, and sebum content of the facial skin of the subjects using the MPA9 multi-functional skin tester produced by Courage Khazaka, Germany, before treatment and 14 days after treatment. The test environment was a dedicated skin function test room with no ventilation, no direct sunlight, a room temperature of 22 - 24°C, and a relative humidity of 45% - 55%. The specific method was that on the day of the test, the subjects did not use any facial topical medications or cosmetics, moisturized their faces routinely, and after resting in the skin function test room for 30 minutes to adapt to the environment, the test was conducted. The researchers tested each of the three indicators at the same detection site of the subjects three times according to the instrument operation regulations, with an interval of about 5 minutes in between, and the average value was taken as the result of this determination.

[0161] 1.6 Subjective evaluation

[0162] A questionnaire survey method was adopted to let the subjects self-evaluate the skin condition of the test site. 1 point means dissatisfied, 2 points means relatively dissatisfied, 3 points means average, 4 points means satisfied, and 5 points means completely satisfied. The number of people with a statistical score > 3 points was the effective number, and the satisfaction rate = (effective number / effective data) × 100%.

[0163] 1.7 Statistical methods

[0164] The data were double-checked and then entered into the SPSS 25.0 software for statistical analysis. Measurement data were expressed as (x ± s), and paired t-tests were used for comparison before and after drug administration in the same group. One-way analysis of variance was used for comparison among multiple groups, and independent sample t-tests were used for pairwise comparison between groups. Count data were expressed as rates, and chi-square tests were used. P < 0.05 indicated that the difference was statistically significant.

[0165] 2 Results

[0166] 2.1 Clinical efficacy

[0167] The changes in the facial skin of the subjects before and after treatment were as Figure 3 shown, Figure 3 (A) is a picture of the skin of the subject before using the essence containing natural plant fermentation broth for treatment, Figure 3 (B) is a picture of the skin of the subject 14 days after using the essence containing natural plant fermentation broth for treatment.

[0168] After statistics, among the 40 subjects 14 days after using the essence containing natural plant fermentation broth for treatment, 20 people showed significant improvement in skin oiliness and greasiness, accounting for 50.00% of the total number of subjects; 15 people were effective, accounting for 37.50% of the total number of subjects, and the total effective rate was 87.50%. This indicates that the essence containing natural plant fermentation broth of the present invention has a good moisturizing and oil-control effect.

[0169] 2.2 Detection indexes of the skin barrier function of the subjects before and after treatment

[0170] The detection indexes of the skin barrier function before and after treatment are shown in Table 8. The data in Table 8 show that, compared with before treatment, the water content of the stratum corneum of the subjects after treatment increased significantly, while the TEWL and sebum amount decreased significantly, with statistical differences (P < 0.01).

[0171] Table 8 Detection indexes of the skin barrier function of the subjects before and after treatment

[0172]

[0173] 2.3 Statistical results of the subjective evaluation of the subjects

[0174] The self-evaluation results of the subjects on the facial skin conditions are shown in Table 9. It can be seen from Table 9 that after 14 days of treatment with the essence containing natural plant fermentation broth of the present invention, the overall skin satisfaction of the subjects was 90.00%.

[0175] Table 9 Self-evaluation results of the subjects on the facial skin conditions

[0176] Evaluation dimension Satisfaction degree (%) Ease of use 95 Moisturizing effect during use 95 Moisturizing effect after 2h of use 90 Oil control effect after 2h of use 85 Improvement effect of skin glossiness 90 Overall satisfaction degree 90

[0177] 2.5 Adverse reactions

[0178] After 14 days of treatment and observation, no one had adverse reactions. All the enrolled subjects completed the treatment of their respective treatment courses. The compliance was good during the whole research period, and there was no dropout during the research process.

[0179] 3 Conclusion

[0180] The research results of this test example show that when the facial skin is of a mixed or oily type, the essence containing natural plant fermentation broth of the present invention has a significant moisturizing and oil-control effect on the facial skin, is non-irritating, the subjects have good tolerance, no side effects occurred in any case, and the compliance is high.

[0181] To sum up, the beneficial effects of the present invention compared with the prior art are as follows:

[0182] (1) The preparation method of the natural plant fermentation broth of the present invention is simple. By adding a mixed enzyme and a mixed bacterium agent successively, the extraction efficiency of the effective components of the natural plant is improved, thereby enhancing the medicinal efficacy of the natural plant fermentation broth. After detection, the microbial indexes of the natural plant fermentation broth prepared by the preparation method of the present invention meet the hygiene standards of cosmetics, and it has high safety. At the same time, compared with the extraction liquids obtained by other extraction methods, after adjusting the crude drug concentration, the natural plant fermentation broth of the present invention has the lightest color and the highest clarity, and is suitable for being added to the cosmetic matrix as a raw material;

[0183] (2) The natural plant fermentation broth of the present invention can be added as a raw material to a cosmetic matrix to prepare a cosmetic for treating oily and sensitive skin. The essence containing the natural plant fermentation broth prepared from the natural plant fermentation broth is non-irritating. Through experiments, it is found that the essence can improve the water-locking and barrier functions of the skin, effectively improve the roughness, glossiness of the facial skin of the subjects, and the skin lactic acid stinging score. It not only has a good soothing effect on allergic skin, but also has a significant moisturizing and oil-control effect in the treatment of mixed and oily facial skin, with good tolerance, no side effects, high compliance, and can treat oily and sensitive skin.

[0184] The above are only some embodiments of the present invention. For those of ordinary skill in the art, without departing from the inventive concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention.

Claims

1. Natural plant fermentation liquid, characterized in that, The invention is prepared with Acanthopanax senticosus, Angelica sinensis, Rosa laevigata, medicinal phyllotoxin, tea leaves, water, mixed enzyme and mixed bacterial agent as raw materials; wherein the mixed enzyme comprises cellulase, pectinase and tannase, and the mixed bacterial agent comprises Lactobacillus plantarum, Bifidobacterium and Lactobacillus acidophilus.

2. The natural plant fermentation liquid according to claim 1, characterized in that: Calculated by weight, the content of Acanthopanax senticosus is 1-5 parts, the content of Angelica sinensis is 1-5 parts, the content of Rosa laevigata is 1-5 parts, the content of Phyllostachys medica is 1-5 parts, the content of tea leaves is 1-5 parts, and the content of water is 6-8 parts.

3. The natural plant fermentation liquid according to claim 2, characterized in that: The mass of the mixed enzyme can be 0.3% to 0.5% of the total mass of Acanthopanax senticosus, Angelica sinensis, Rosa laevigata, Phyllostachys medica, tea leaves and water; wherein the mass ratio of cellulase, pectinase and tannase in the mixed enzyme can be 1:1:

2.

4. The natural plant fermentation liquid according to claim 2, characterized in that: The dosage of the mixed bacterial agent can be 0.5% to 0.7% of the total mass of Acanthopanax senticosus, Angelica sinensis, Rosa laevigata, Phylloscopus medica, tea leaves and water; the mass ratio of Lactobacillus plantarum, Bifida saccharomyces cerevisiae and Lactobacillus acidophilus in the mixed bacterial agent is 1:1:

1.

5. The natural plant fermentation liquid according to any one of claims 2 to 4, characterized in that: The raw materials of the natural plant fermentation liquid also include sucrose and rice flour. The amount of sucrose is 7% to 8% of the total mass of the acanthopanax senticosus, angelica sinensis, rose hip, medicinal pore fungus, tea leaves and water. The amount of rice flour is 3% to 5% of the total mass of the acanthopanax senticosus, angelica sinensis, rose hip, medicinal pore fungus, tea leaves and water.

6. The method for preparing the natural plant fermentation liquid according to any one of claims 1 to 5, characterized in that: The following steps are involved: (1) mixing and grinding Acanthopanax senticosus, Angelica sinensis, Rosa laevigata, Facies medica, and tea leaves with water to obtain a slurry; (2) adding a mixed enzyme to the slurry at 50 to 60° C. to perform enzymolysis to obtain an enzymolysis solution; (3) dissolving sucrose and rice flour in the enzymolysis solution to obtain a mixed solution; (4) filtering the mixed solution and sealing the filtrate, and sterilizing the sealed filtrate with high pressure steam, cool down; (5) activating Lactobacillus plantarum, bifid yeast and Lactobacillus acidophilus, and mixing the activated Lactobacillus plantarum, bifid yeast and Lactobacillus acidophilus to obtain a mixed bacterial agent; (6) inoculating the mixed bacterial agent into the cooled filtrate; (7) Ferment the inoculated filtrate at 35-50° C. for 6-8 days to obtain the product.

7. The preparation method according to claim 6, characterized in that: In step (4), the filtering method is to filter the mixed solution using clean gauze; The method for sealing the filtrate is to dispense the filtrate into a conical flask and then seal the conical flask mouth with a sterile culture container sealing film.

8. The preparation method according to claim 6, characterized in that: In step (5), the activation method is to take out the cryogenic storage tubes respectively containing Lactobacillus plantarum, Saccharomyces cerevisiae and Lactobacillus acidophilus from the low temperature environment, and then gently shake the cryogenic storage tubes in a water bath environment at 36-37°C until the ice in the cryogenic storage tubes is completely dissolved.

9. Use of the natural plant fermentation liquid according to any one of claims 1 to 5 in the preparation of cosmetics, wherein the cosmetics have at least one of the following functions: (1) Improve the skin's water-locking function; (2) Improve skin barrier function; (3) Improve skin roughness and glossiness; (4) Antihistamine, anti-allergic and soothing effects; (5) Moisturizing and oil controlling.

10. A cosmetic, characterized in that: A cosmetic containing the natural plant fermentation liquid according to any one of claims 1 to 5, and having at least one of the following functions: (1) Improve the skin's water-locking function; (2) Improve skin barrier function; (3) Improve skin roughness and glossiness; (4) Antihistamine, anti-allergic and soothing effects; (5) Moisturizing and oil controlling.

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