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Denitrifying phosphorus removal bacteria bacillus cereus H-hrb01 and screening method and application

A technology of denitrifying phosphorus-accumulating bacteria and Bacillus cereus, which is applied in chemical instruments and methods, biochemical equipment and methods, and methods based on microorganisms, can solve the problems of limited practical application research and single strains, and achieve strong The effect of phosphorus capacity on denitrifying bacteria

Active Publication Date: 2012-12-19
HIT YIXING ACAD OF ENVIRONMENTAL PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for effective treatment of wastewater containing harmful substances like phosphonium or other compounds that may be found in waterways such as septic tanks. It uses various types of organisms called Bacillussynucleate (Bcc) which help reduce pollution from these sources. By combining this with an indicator controlling process, it becomes more efficient at treating contaminated waters compared to traditional methods involving biological processes alone.

Problems solved by technology

This patented technical solution described by this patents addresses two issues: firstly, there may exist contradicting factors that make removing nitrosis or phosphorous effectively challenges; secondly, existing methods have limitations for effective treatment of waste waters containing both types of contaminants.

Method used

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  • Denitrifying phosphorus removal bacteria bacillus cereus H-hrb01 and screening method and application
  • Denitrifying phosphorus removal bacteria bacillus cereus H-hrb01 and screening method and application
  • Denitrifying phosphorus removal bacteria bacillus cereus H-hrb01 and screening method and application

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specific Embodiment approach 1

[0022] Specific implementation mode 1: The activated sludge at the end of the anoxic section of a certain period when the SBR reactor is running stably is used as the separation sludge. Make a bacterial suspension from the activated sludge and add it to the denitrification medium. After the medium is solidified, invert the culture dish and incubate it in a constant temperature incubator at 30°C for 2 to 3 days. Select typical colonies with different shapes and clear colonies. , mark and pick a single colony on the plate medium for three-section line separation, repeat 3 to 4 times to a single colony with the same colony characteristics, and finally pick a single colony and transfer it to the prepared test tube slant for future use. All manipulations were performed under sterile conditions. The isolated strains were tested for phosphorus uptake in phosphorus-rich medium, and nitrate reduction gas production test, heterochromatic particle staining (methylene blue staining method...

specific Embodiment approach 2

[0027] By amplifying the 16S rDNA of the strain, the 16S rDNA sequence with a length of 1518 bp was obtained. The bacterial general primers BA101F 5'-TGGCGGACGGGTGAGTAA-3' and BA534R 5'-ATTACCGCGGCTGCTGCTGCTGG-3' were used as PCR primers. The amplification reaction was performed with a PCR instrument (GeneAmp, PCR System 9700). The amplification reaction was performed with a PCR instrument (GeneAmp, PCR System 9700). PCR reaction system (25 μL): 2.5 μL 10×PCR Buffer, 2 μL dNTPs (concentration: 2.5 mmol / L), 0.5 μL each of the two primers, 0.5 μL DNA template, 0.5 μL r Taq DNA polymerase (5U / μL), sterile deionized water 18.5μL. Negative samples were set as a reference in each PCR reaction, that is, DNA templates were replaced with sterile ultrapure water. PCR amplification program: The PCR amplification program for the full sequence of 16S rRNA is: 95°C pre-denaturation for 5 minutes, 94°C for 1 minute, 58°C for 30 seconds, 72°C for 3 minutes, a total of 30 cycles, and fina...

specific Embodiment approach 3

[0054] Preparation of strains Bacillus cereus Resting cells of H-hrb01: the cultured strain Bacillus cereus H-hrb01 was centrifuged at 8000 r / min at 4°C for 10 min, washed 3 times with phosphate buffer, and the pellet was resuspended with the same buffer to make the concentration of resting cells 10 mg / l, and frozen at 4°C. Standby, that is, the prepared strain Bacillus cereus Resting cells of H-hrb01.

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Abstract

The invention discloses denitrifying phosphorus removal bacteria bacillus cereus H-hrb01, and belongs to the technical fields of biological engineering and environmental engineering, wherein bacillus cereus H-hrb01 belongs to bacillus genus by identification and is conserved in the general microbiology centre of the China committee for culture collection of microorganisms, the conservation date is March 27th 2012, and the conservation number is CGMCC NO.5939; bacillus cereus H-hrb01 can take various organic matters as carbon sources, has good denitrifying and phosphorus-removing efficiency, and can be used for sewage biological denitrification and dephosphorization process; bacillus cereus H-hrb01 is activated and cultivated, and fed into artificial wastewater to mix and then introduced into an SBR (styrene butadiene rubber) biofortification device, and each effluent index of the reactor is superior to that of a contrast system which is not fed with the bacterial strain. The removal rates of the COD (chemical oxygen demand), ammonia nitrogen and dissoluble phosphorus in the sewage are respectively up to 93.90%, 97.70% and 96.25%.

Description

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Claims

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Application Information

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Owner HIT YIXING ACAD OF ENVIRONMENTAL PROTECTION
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