New type marine microorganism of lysozyme and bacillus 5-12 of producing lysozyme in high yield

A technology of marine microorganisms, bacillus, applied in the field of marine microorganisms

Inactive Publication Date: 2005-10-26
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a single lysozyme has not been reported
As a kind of protein, lysozyme can be used in cosmetics without restriction in dosage, and it c

Method used

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  • New type marine microorganism of lysozyme and bacillus 5-12 of producing lysozyme in high yield
  • New type marine microorganism of lysozyme and bacillus 5-12 of producing lysozyme in high yield
  • New type marine microorganism of lysozyme and bacillus 5-12 of producing lysozyme in high yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0140] Preliminary screening: Spread the appropriately diluted sea mud suspension from the East China Sea on a plate containing Staphylococcus aureus, incubate at a constant temperature of 30°C for 2 days, inoculate the strain with a larger transparent circle into the liquid fermentation medium for secondary screening, and select the fermentation broth The strains with strong lytic activity were stored in milk tubes, recorded as S-12, wherein the liquid fermentation medium % (wt): beef extract powder 0.3, peptone 0.5, NaCl 0.5, tap water preparation.

[0141] The strain S-12 was first protoplastized and the cell concentration was adjusted. First, it was irradiated with guanidine nitrite for 20 hours, and then irradiated with external light for 1 minute. It was spread on a regeneration plate and cultured for screening. Finally, the mutant strain of strain S-12-86 was obtained.

[0142] Preparation of crude enzyme solution:

[0143] Take one loop of the S-12-86 mutant strain pre...

Embodiment 2

[0155] The method step of embodiment 2 is the same as that of embodiment 1, and the difference is that the fermentation and culture conditions of the conical flask are to use dextrin 2%, peanut meal 2% as carbon source, and NH 4 CL 1%, (NH 4 ) 2 SO 4 1%. As a nitrogen source, the initial pH value is 7, inoculated with 6% of 20h-old seeds, and cultivated in a rotary shaker for 27h under the conditions of 25°C and 250r / min.

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Abstract

The invention relates to a bacillus S-12 of high yield ocean microorganism lysozyme which separated from sea mud of The East China Sea.It is conserved at the type cultivating matter protect center of HuHan China, with CCTCC NO:M204089.The ratio that this S-12 and Bacillus bacterial homology is between 98% and 99%.It is a new fungus ilk with a filiation.The bacteriolysin molecular weight produced by this fungus is 39KD,consisted of 354 aminophenolls. It can be widely used in cosmetic, medicament, disinfector and breeding fields.

Description

technical field [0001] The invention relates to marine microorganisms, in particular to a novel marine microorganism lysozyme and its high-yield lysozyme-producing bacillus S-12. Background technique [0002] The ocean is vast and rich in microbial resources. However, due to the unique environment of the ocean, including high salinity, high pressure, low nutrition, and low temperature, marine microorganisms have many specificities that are different from terrestrial microorganisms, such as difficult cultivation, diverse forms, and preservation. It is not conducive to the in-depth research and development of marine microorganisms, so it is urgent to establish some simple, convenient, easy-to-operate and to a certain extent more scientific, more accurate and faster to find and isolate Obtain useful microbial strains and lay the foundation for the development and utilization of marine microbial resources. At present, macromolecular rRNA has become a molecular index, which is w...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 孙谧王跃军张琇袁翠
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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