12 results about "Bacillus species" patented technology

This invention discloses a Bacillus NWAFU20-20 and its application in the prevention and control of plant diseases and the promotion of plant growth, belonging to the field of microbial application technology, and particularly relating to a Bacillus ( Bacillus Bacillus sp. NWAFU20-20 was deposited at the China Center for Type Culture Collection (CCTCC) on January 23, 2026, with accession number CCTCC NO: M 2026209. This Bacillus sp. NWAFU20-20 is used in the control of plant diseases. The Bacillus sp. NWAFU20-20 provided by this invention can alleviate garlic leaf blight, significantly promote the growth of garlic, cucumber, and tomato seedlings, and exhibits good broad-spectrum resistance to plant diseases.

The invention discloses a method for preventing and controlling epimedium colletotrichum gloeosporioides by bacillus velezensis and application. The method comprises the following steps: step 1, preparing a bacillus velezensis seed solution; step 2, preparing a bacillus velezensis sterile fermentation broth; step 3, preparing raw materials; 4, preparing a biopesticide mother solution; step 5, filtering and sub-packaging; step 6, applying a biopesticide; the biological pesticide is used for preventing and controlling the epimedium colletotrichum gloeosporioides, and the antibacterial extract is matched with the bacillus velezensis sterile fermentation liquor with extremely high inhibition on the epimedium colletotrichum gloeosporioides to serve as a core antibacterial component to synergistically inhibit growth of pathogenic bacteria and disease diffusion; according to the biopesticide, the stability of a pesticide system is improved through the stabilizing aid, the active ingredients are promoted to be uniformly dispersed through the dispersing aid, the spreadability of leaves is enhanced, the acting time of the active ingredients on the leaves is prolonged through the moisturizing aid, the raw materials are green and environment-friendly, pollution to plants and the ecological environment is avoided, and high prevention and control efficiency and environmental safety are both considered.

The invention relates to the technical field of microorganisms and biological medicines, in particular to triple probiotics as well as a preparation method and application thereof, and the triple probiotics comprise at least one probiotic of bacillus, lactobacillus acidophilus and bifidobacterium longum. The triple probiotics provided by the invention form an'oxygen consumption-enzyme production-acid production 'synergistic chain, and through synergistic degradation of uric acid, inhibition of excessive growth of conditioned pathogens and enhancement of intestinal barrier functions, the blood uric acid level of patients with asymptomatic hyperuricemia is reduced, the alpha-diversity of intestinal tracts is improved, the abundance of butyric acid producing bacteria is improved, and the content of the butyric acid producing bacteria is improved. The ratio of escherichia coli to shigella is reduced; sIBO is effectively relieved or reversed, and digestive tract symptoms such as abdominal distension and exhaust are reduced. In addition, the triple probiotics avoid liver and kidney toxicity caused by chemical drugs, and are suitable for long-term administration.

Disclosed herein are compositions comprising A) compound of Formula One (F1) or Formula Two (F2); and B) biological agent selected from the group consisting of Bacillus species, Baclovirus species, Pseudomonas species, Beauveria species, Mycobacterium species, Mycorrhizal fungi, Trichoderma species, and Metarhizium species.

This invention discloses a method for improving the high yield of extracellular polysaccharides from Bacillus belye on agar plates based on agricultural waste, belonging to the fields of microbial fermentation technology and resource utilization of agricultural waste. The method involves inoculating Bacillus belye seed culture into agar plates containing agricultural waste, culturing at 36-38°C to obtain fermentation products, and extracting the extracellular polysaccharides from the fermentation products. The agar plate containing agricultural waste comprises: peptone 9-11 g / L, yeast extract 4.5-5.5 g / L, NaCl 9.5-10.5 g / L, agar 17-19 g / L, agricultural waste powder 10-15 g / L, and pH 7.1-7.3. This method solves the problems of low yield, high cost, and high energy consumption associated with traditional liquid shake-flask culture, while simultaneously achieving high-value utilization of agricultural waste.

This invention provides a Bacillus strain and its applications, belonging to the field of microbial germplasm resource development and utilization technology. The Bacillus strain of this invention includes *Bacillus belyssae* BEV2 and / or *Bacillus amyloliquefaciens* BAM7. The *Bacillus belyssae* BEV2 has the accession number CGMCC NO. 26741, and the *Bacillus amyloliquefaciens* BAM7 has the accession number CGMCC NO. 26742. The Bacillus strain of this invention has a significant inhibitory effect on *Potato scab* pathogens and promotes potato growth. It also has significant inhibitory effects on *Anthracis coccidioides*, *Helicobacter pylori*, *Alternaria alternata*, *Rhizoctonia solani*, and *Fusarium oxysporum*, and also has a phosphorus-solubilizing effect.

The present invention relates to the use of a particular bacterial species as a biosolution in the production and cultivation of tomatoes. More particularly, the invention relates to the use of a Bacillus siamensis strain, deposited with the National Collection of Cultures of Microorganisms (CNCM) under the accession number CNCM I-5921, in the stimulation of the development and growth of seeds and of tomato plants or the improvement of the yields of tomato crops, and in the improvement of the resistance and tolerance of seeds, tomato plants and tomato crops to abiotic stress.

The application provides an animal microecological preparation with stress resistance and a preparation method thereof, and belongs to the technical field of microecological preparations, and comprises the following steps: culturing Bacillus subtilis and lactic acid bacteria to obtain Bacillus subtilis seed liquid and lactic acid bacteria seed liquid, carrying out aerobic fermentation on the Bacillus subtilis seed liquid, and adding the lactic acid bacteria seed liquid to carry out micro-aerobic fermentation; culturing Saccharomyces cerevisiae to obtain yeast seed liquid; after the fermentation product and the yeast seed liquid are mixed, centrifugation and freeze-drying are sequentially carried out to obtain freeze-dried bacterial powder; the freeze-dried bacterial powder, prebiotics, chlorogenic acid, L-glutamine, yeast beta-glucan and deionized water are mixed to obtain a core solution; after the core solution and a sodium alginate solution are mixed, the mixture is dropped into a calcium chloride solution to carry out cross-linking, the mixture is soaked in a chitosan solution, and then the mixture is soaked in a trisodium phosphate solution to carry out cross-linking, and finally, the mixture is subjected to spray drying. Through the composite embedding of multi-strain synergistic fermentation and functional components, the animal microecological preparation is improved in the stress resistance.

The invention discloses a preparation method of a microbial fertilizer, and belongs to the technical field of microbial fertilizers, the preparation method comprises the following steps: preparing a bacillus subtilis seed solution, preparing a paenibacillus polymyxa seed solution, treating corn steep liquor, preparing a fermentation medium, and fermenting; the step of treating the corn steep liquor comprises the following steps: mixing the corn steep liquor, distilled water and sodium carboxymethyl cellulose, stirring at room temperature, adjusting the pH value to 6.8-7.2, then adding calcium chloride, stirring, adding sodium carbonate, and stirring; the preparation of the fermentation culture medium comprises the following steps: mixing glucose, treated corn steep liquor, composite calcium carbonate, yeast extract powder, ammonium sulfate, disodium hydrogen phosphate, dipotassium phosphate, calcium chloride, magnesium sulfate, potassium chloride, ferrous sulfate, manganese sulfate and distilled water, adjusting the pH value to 6.8-7.2, and sterilizing; the number and the forming rate of spores in the prepared microbial fertilizer are high, and the prepared microbial fertilizer is good in quality stability, planting effect and appearance stability.

The invention provides a heavy metal contaminated soil leaching remediation method, and relates to the technical field of soil remediation. The leaching remediation method for the heavy metal contaminated soil comprises the following steps that the heavy metal contaminated soil to be remedied is air-dried, ground and then sieved through a 2 mm sieve, leacheate is added in proportion, oscillation leaching and separation are conducted, leaching and separation operation is repeatedly conducted, the leacheate is recycled and regenerated to obtain regenerated leacheate, the soil is washed with deionized water to remove residual leacheate, air drying is conducted, and the soil is obtained. And repairing is completed. The leacheate is prepared from compound bacteria fermentation liquor, rhamnolipid, sodium humate and water; the compound bacteria fermentation liquor is compound fermentation liquor of bacillus seed liquor and aspergillus niger seed liquor. According to the method, an organic acid complexing dissolution-surfactant solubilizing leaching promoting-humic acid complexing stable synergistic mechanism is constructed, efficient leaching remediation of the soil compositely polluted by multiple heavy metals such as lead, cadmium, copper and zinc is achieved, meanwhile, the leaching liquid can be regenerated and recycled after being subjected to pH adjustment and precipitation treatment, and the method has a good application prospect.

The present invention relates to a composition for the prevention, alleviation, or treatment of fibrotic diseases, containing a Bacillus species strain, a Bacillus species strain spore, each expressing superoxide dismutase, and / or superoxide dismutase. When orally administered, the composition allows the polypeptide with superoxide dismutase activity to remove reactive oxygen species in vivo, thus being helpful for the prevention, improvement, or treatment of fibrotic diseases such as pulmonary fibrosis or hepatic fibrosis.

A method for improving pond water quality used in aquaculture applications involves adding active bacteria to the pond water, preferably germinating from spores on-site, using a germination nutrient composition and an incubation method to increase spore germination efficiency. This is combined with a nitrification-enhancing agent such as calcium carbonate or calcified algae, and an optional surface area modifier such as calcified algae or plastic or metal particles or fragments. The germination nutrient composition comprises L-amino acids, D-glucose or D-fructose, a phosphate buffer, an industrial preservative, and may include bacterial spores (preferably of one or more Bacillus species) or these can be combined separately for germination.The incubation method comprises heating a germinating composition of nutrients and bacterial spores to a temperature range of 35°C to 60°C for approximately 2 to 60 minutes to produce a solution of incubated bacteria that is discharged to the aquaculture application.