40 results about "Bacillus species" patented technology

The present invention relates to the field of microbial technology, and specifically discloses a new broad-spectrum disease-resistant Bacillus species, a fungicide compound, and applications thereof. The new broad-spectrum disease-resistant Bacillus species is Bacillus species 129-52, which was deposited on April 1, 2025, at the General Microbiology Center of the China Culture Collection Administration, located at the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No. 34059, and is classified as Bacillus sp.; the strain 129-52 of the present invention has a living inhibition rate of 66.82% on branches with apple rot disease. After using fungicide-drug synergistic treatment, it was found that the inhibition rate of two tested drugs, carbendazim and pyraclostrobin, on apple rot disease reached more than 80% after the dosage of the two tested drugs, carbendazim and pyraclostrobin, was reduced by half. The fungicide-drug synergistic treatment can effectively achieve the purpose of reducing the dosage, maintaining the effect, and efficiently preventing and controlling apple rot disease.

A spore-forming Bacillus species, and more particularly, a Bacillus velezensis strain identified as BV379 is provided. Compositions comprising BV379 and methods of using the same are also provided.

Exemplary embodiments of the present disclosure are directed towards a carrier-based agricultural biofertilizer composition, comprising an isolated Bacillus megaterium strain CGAPGPBBS-034 having the deposit accession number KY495205; and an agriculturally acceptable liquid formulation with a stabilizer to provide stability and support for at least one of the Bacillus species, thereby enhancing the growth and overall health of the plants.

The present invention relates to the field of microbial technology, and specifically discloses a new biocontrol bacillus species, a bacterium-drug compound, and applications thereof. The new biocontrol bacillus species is Bacillus species 122-9, which was deposited on April 1, 2025, at the General Microbiology Center of the China Culture Collection Administration, located at the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No. 34057. It is classified as Bacillus sp.; the strain 122-9 of the present invention has a living inhibition rate of 79% on branches with apple rot, and has high compatibility with tebuconazole, carbendazim, and pyraclostrobin. After using bacterium-drug synergistic treatment, it was found that the inhibition rate of the three tested drugs on apple rot reached more than 80% even when the dosage was halved.

The invention belongs to the field of veterinary drugs, and particularly relates to a probiotic fermented traditional Chinese medicine composition for preventing and controlling yellow scour of piglets and a preparation method thereof, and the probiotic fermented traditional Chinese medicine composition comprises 100 + / -2 parts of radix sophorae flavescentis, 10 + / -2 parts of fructus forsythiae, 100 + / -2 parts of liquorice, 200 + / -2 parts of dandelion, 100 + / -2 parts of coptis chinensis and 5-20 parts of bacillus velezensis SXDYYB3 preparation. The bacillus velezensis SXDYYB3 preparation is prepared by inoculating a seed solution of the bacillus velezensis SXDYYB3 into a culture medium for fermentation, the bacillus velezensis SXDYYB3 is preserved in the China Center for Type Culture Collection, and the preservation number of the bacillus velezensis SXDYYB3 is CCTCC NO: M 2025428. Two weeks before farrowing of sows, the probiotic fermented traditional Chinese medicine composition for preventing and controlling piglet yellow scour is mixed with feed to feed the sows, and the prevention effect can be achieved; after piglets are born and before yellow scour does not appear, the yellow scour of the piglets can be prevented by feeding the piglets with the medicine mixed with feed, the yellow scour of the piglets can be cured by increasing the dosage after the piglets are attacked, and the prevention and cure rate can reach 70-90%.

This invention discloses a Bacillus NWAFU20-20 and its application in the prevention and control of plant diseases and the promotion of plant growth, belonging to the field of microbial application technology, and particularly relating to a Bacillus ( Bacillus Bacillus sp. NWAFU20-20 was deposited at the China Center for Type Culture Collection (CCTCC) on January 23, 2026, with accession number CCTCC NO: M 2026209. This Bacillus sp. NWAFU20-20 is used in the control of plant diseases. The Bacillus sp. NWAFU20-20 provided by this invention can alleviate garlic leaf blight, significantly promote the growth of garlic, cucumber, and tomato seedlings, and exhibits good broad-spectrum resistance to plant diseases.

The invention discloses a method for preventing and controlling epimedium colletotrichum gloeosporioides by bacillus velezensis and application. The method comprises the following steps: step 1, preparing a bacillus velezensis seed solution; step 2, preparing a bacillus velezensis sterile fermentation broth; step 3, preparing raw materials; 4, preparing a biopesticide mother solution; step 5, filtering and sub-packaging; step 6, applying a biopesticide; the biological pesticide is used for preventing and controlling the epimedium colletotrichum gloeosporioides, and the antibacterial extract is matched with the bacillus velezensis sterile fermentation liquor with extremely high inhibition on the epimedium colletotrichum gloeosporioides to serve as a core antibacterial component to synergistically inhibit growth of pathogenic bacteria and disease diffusion; according to the biopesticide, the stability of a pesticide system is improved through the stabilizing aid, the active ingredients are promoted to be uniformly dispersed through the dispersing aid, the spreadability of leaves is enhanced, the acting time of the active ingredients on the leaves is prolonged through the moisturizing aid, the raw materials are green and environment-friendly, pollution to plants and the ecological environment is avoided, and high prevention and control efficiency and environmental safety are both considered.

The invention relates to the technical field of microorganisms, and particularly discloses a novel broad-spectrum disease-resistant bacillus strain, a bacterial-drug compound agent and application of the novel broad-spectrum disease-resistant bacillus strain and the bacterial-drug compound agent. The broad-spectrum disease-resistant bacillus new species are bacillus new species 129-52 and are preserved in China General Microbiological Culture Collection Center on April 1, 2025, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the preservation number is CGMCC No.34059, and the broad-spectrum disease-resistant bacillus new species are classified and named as bacillus sp. The living body inhibition rate of the bacterial strain 129-52 on branches with apple rot reaches 66.82%, after synergistic treatment of bacteria and drugs, the inhibition rate of the bacterial strain 129-52 on the apple rot also reaches 80% or above after the use of two test drugs, namely carbendazim and pyraclostrobin, is halved, and the bacteria and drug synergism can effectively achieve the purposes of drug reduction, persistent effect and efficient prevention and control of the apple rot.

The invention relates to the technical field of microorganisms and biological medicines, in particular to triple probiotics as well as a preparation method and application thereof, and the triple probiotics comprise at least one probiotic of bacillus, lactobacillus acidophilus and bifidobacterium longum. The triple probiotics provided by the invention form an'oxygen consumption-enzyme production-acid production 'synergistic chain, and through synergistic degradation of uric acid, inhibition of excessive growth of conditioned pathogens and enhancement of intestinal barrier functions, the blood uric acid level of patients with asymptomatic hyperuricemia is reduced, the alpha-diversity of intestinal tracts is improved, the abundance of butyric acid producing bacteria is improved, and the content of the butyric acid producing bacteria is improved. The ratio of escherichia coli to shigella is reduced; sIBO is effectively relieved or reversed, and digestive tract symptoms such as abdominal distension and exhaust are reduced. In addition, the triple probiotics avoid liver and kidney toxicity caused by chemical drugs, and are suitable for long-term administration.

The invention relates to a microbial seed treatment agent and application thereof in agricultural production, and belongs to the field of research and development of agricultural microbial products. The bacillus Bs916 has a relatively strong effect of inhibiting pathogenic bacteria of the rice bakanae disease. The invention provides a seed treatment agent which is prepared by taking Bs916 living spores as active ingredients and mixing auxiliaries, the seed treatment agent is red suspension liquid, the spore content is more than or equal to 3 * 10 < 9 > CFU / mL, the suspension rate is more than 80%, and the pH is 6.0-7.0. The bacillus Bs916 seed treating agent has the effects of promoting rice seed germination and seedling growth and preventing and treating rice bakanae disease. When the seed treatment agent is respectively mixed with tebuconazole (250mg / L), validamycin (50mg / L) and agricultural resistance 120 (200mg / L), the effects of preventing and treating the rice bakanae disease are respectively 87.54 percent, 86.74 percent and 88.05 percent. The invention discloses a microbial seed treatment agent capable of promoting growth and preventing and treating rice bakanae disease, which can stabilize the prevention effect, reduce the dosage of chemical pesticide and reduce pesticide residue when being mixed with chemical pesticide, and has potential application value and market development prospect in agricultural production.

Disclosed herein are compositions comprising A) compound of Formula One (F1) or Formula Two (F2); and B) biological agent selected from the group consisting of Bacillus species, Baclovirus species, Pseudomonas species, Beauveria species, Mycobacterium species, Mycorrhizal fungi, Trichoderma species, and Metarhizium species.

This invention provides a Bacillus and its applications. The Bacillus was deposited at the Guangdong Provincial Microbial Culture Collection Center on June 6, 2025, with accession number GDMCC No. 66483. One application of the Bacillus is to prepare preparations that promote plant growth and / or disease resistance. Another application is to use the Bacillus to inhibit microorganisms. This Bacillus can effectively and persistently inhibit the growth of plant pathogens and can be used as a broad-spectrum antibacterial biological control agent to enhance plant resistance and promote plant growth.

This invention discloses a Bacillus species and its applications, belonging to the field of microbial technology. The Bacillus species is a new species of the genus Bacillus (Bacillus sp. RP12), with the strain name RP12 and preservation number CCTCC No: M20221018. The Bacillus RP12 provided by this invention not only broadly antagonizes kiwifruit canker pathogens in multiple kiwifruit producing areas in China, and can be widely used to control kiwifruit canker in major kiwifruit producing areas of China; but also broadly antagonizes various plant pathogenic fungi, and can be used to control various plant diseases such as kiwifruit postharvest rot and wheat stem rot.

This invention discloses a method for improving the high yield of extracellular polysaccharides from Bacillus belye on agar plates based on agricultural waste, belonging to the fields of microbial fermentation technology and resource utilization of agricultural waste. The method involves inoculating Bacillus belye seed culture into agar plates containing agricultural waste, culturing at 36-38°C to obtain fermentation products, and extracting the extracellular polysaccharides from the fermentation products. The agar plate containing agricultural waste comprises: peptone 9-11 g / L, yeast extract 4.5-5.5 g / L, NaCl 9.5-10.5 g / L, agar 17-19 g / L, agricultural waste powder 10-15 g / L, and pH 7.1-7.3. This method solves the problems of low yield, high cost, and high energy consumption associated with traditional liquid shake-flask culture, while simultaneously achieving high-value utilization of agricultural waste.

The present application relates to the field of biotechnology, and specifically to a method for preparing a mixed bacterial agent based on plant endophytes, the method comprising: culturing Bacillus polymyxa and Bacillus firmus in an activation medium, inoculating the activated two types of Bacillus single colonies into seed culture medium respectively, shaking and culturing in a shake bottle, and inoculating two types of Bacillus seed liquid respectively for fermentation; when the fermentation monitors the maximum bacterial concentration, stopping the feeding, adjusting the ventilation, dissolved oxygen and rotation speed, and continuing the fermentation, during the fermentation process of the mixed bacterial agent of the two types of Bacillus, collecting the mixed bacterial agent images and fermentation monitoring data at each moment to adjust the fermentation time, mixing the two types of Bacillus mixed fermentation liquid with a carrier and a freeze-drying protective agent, centrifuging, precipitating, precooling, and freeze-drying operations to obtain a mixed bacterial agent. The present application aims to adjust the fermentation time of the mixed bacterial agent and improve the stability and stress resistance of the mixed bacterial agent.

The invention discloses a method for preparing lactic acid by using straws, which comprises the following steps: stirring straws, water and cellulase at 50 DEG C for pre-enzymolysis, inoculating a bacillus coagulans MY5 seed solution, and fermenting; after fermentation, supplementing the enzymatic hydrolysate, and fermenting; or, straw is added after fermentation, a bacillus coagulans MY5 seed solution is inoculated, and fermentation is carried out. According to the method, the problems that an existing process is limited to a single fermentation mode, and efficiency loss is caused by space-time separation of saccharification and fermentation are solved, glucose and xylose can be utilized at the same time by adopting the adaptively evolved bacillus coagulans MY5, an exogenous nitrogen source does not need to be added, and the yield of lactic acid can reach 101.48 g / L through a delayed fermentation strategy of inoculation before secondary feeding.

The invention relates to the technical field of valine, in particular to a preparation method of high-purity valine. The preparation method of the high-purity valine comprises culture, fermentation for acid production, purification and post-treatment, and the specific operation of fermentation for acid production comprises the following steps: inoculating a bacillus seed solution obtained after culture into a fermentation culture medium, and fermenting for a period of time in an environment with the ventilatory capacity of 1-1.2 vvm and the rotating speed of 150-180r / min, so as to obtain the high-purity valine. And then inoculating the culture medium with an escherichia coli seed solution obtained after culture, adjusting the ventilatory capacity to 0.2-0.3 vvm, adjusting the rotating speed to 80-100 r / min, and continuing fermentation to obtain a valine fermentation solution. The purity of the high-purity valine obtained by the preparation method disclosed by the invention is not lower than 99.955%.

The preparation technology comprises the following steps: taking radix astragali decoction pieces and soybeans according to a certain proportion, soaking the radix astragali decoction pieces and soybeans in distilled water, adding hot water, and performing pulping so as to obtain primary pulp; performing high-pressure sterilization on the primary slurry at a certain temperature to obtain secondary slurry; inoculating streptococcus non-lactolyticus into a first culture medium, and carrying out subculture on a plurality of generations of strains to obtain streptococcus non-lactolyticus strains; inoculating bacillus subtilis to a second culture medium for culturing to obtain a bacillus subtilis strain; mixing the secondary slurry with a fermentation culture medium according to a set proportion, inoculating streptococcus non-lactolyticus at the temperature of 30-38 DEG C and the speed of 100-150 rpm, culturing for 12-24 hours, inoculating bacillus subtilis at the temperature of 30-38 DEG C and the speed of 100-150 rpm, culturing for 12-24 hours, and fermenting to obtain a third slurry; and centrifuging the third-stage slurry through a centrifugal machine, and canning under a sterile condition to obtain the astragalus membranaceus soybean milk fermented beverage.

This invention provides a Bacillus strain and its applications, belonging to the field of microbial germplasm resource development and utilization technology. The Bacillus strain of this invention includes *Bacillus belyssae* BEV2 and / or *Bacillus amyloliquefaciens* BAM7. The *Bacillus belyssae* BEV2 has the accession number CGMCC NO. 26741, and the *Bacillus amyloliquefaciens* BAM7 has the accession number CGMCC NO. 26742. The Bacillus strain of this invention has a significant inhibitory effect on *Potato scab* pathogens and promotes potato growth. It also has significant inhibitory effects on *Anthracis coccidioides*, *Helicobacter pylori*, *Alternaria alternata*, *Rhizoctonia solani*, and *Fusarium oxysporum*, and also has a phosphorus-solubilizing effect.

A method for producing polyglutamic acid by fermenting a glutamic acid extract, which method comprises: S1: inoculating a salt-tolerant bacillus seed solution into a fermentation tank containing a fermentation culture medium at an inoculation amount of 20%-25% to perform fermentation, with the liquid loading volume of the fermentation culture medium being 5 L, the culture temperature being 36-38°C, the stirring speed being 350-380 rpm / min and the culture time being 36-42 h, and collecting a polyglutamic acid fermentation liquid; and S2: extracting polyglutamic acid from the polyglutamic acid fermentation liquid by means of using a salt extraction method and a pH adjustment method. The fermentation culture medium has the following formula per 1 L: 15%-20% of concentrated molasses, 2%-5% of corn steep liquor, 55%-60% of a glutamic acid extract and 0.3%-0.5% of K2HPO4, which are dissolved in distilled water such that the system has a total volume of 1 L and a pH of 6.0-6.5. In the method for producing polyglutamic acid by fermenting a glutamic acid extract provided by the present application, the glutamic acid extract is used as the main raw material for the fermentation and production of polyglutamic acid for the first time in the industry, such that nutrient components in the glutamic acid extract are fully utilized, and high-quality utilization of the glutamic acid extract is realized.

To provide a formulation including components contributing to epidermal and dermal health.SOLUTION: The present invention provides a bacterium belonging to Staphylococcus epidermidis species, strain BG-S174, deposited as accession number NITE P-04075, which enhances expression of the ceramide synthase 3 gene and also enhances expression of the hyaluronan synthase 3 gene. A formulation prepared using this strain can enhance expression of the ceramide synthase 3 gene and also enhance expression of the hyaluronan synthase 3 gene.SELECTED DRAWING: None

The invention discloses a preparation method of thyme fermentation liquor, and belongs to the technical field of microbial fermentation. The preparation method comprises the following steps: (1) grinding thyme into powder; (2) sterilizing: adding thyme powder into water with the mass 20-30 times that of the thyme powder, heating to 50-60 DEG C, adding 20-50g / L of glucose, 5-20g / L of peptone, 2-10g / L of yeast extract, 1-5g / L of monopotassium phosphate and 1-5g / L of calcium carbonate, stirring for dissolving, sterilizing for 20 minutes under the conditions of 121 DEG C and 0.1 Mpa, and cooling to obtain a culture solution; and (3) fermentation: firstly inoculating a bacillus subtilis seed solution into the culture solution, carrying out aerated stirring fermentation at 29-32 DEG C for 16-24 hours, then inoculating a saccharomyces cerevisiae seed solution into the fermentation solution, continuously carrying out aerated stirring fermentation at 32-35 DEG C for 10-20 hours, sterilizing at 95-100 DEG C for 8-10 minutes, and terminating the fermentation to obtain the thyme fermentation solution.

The invention discloses a method for segmented fermentation of soybean meal feed by using bacillus coagulans, and belongs to the technical field of feed processing. The preparation method comprises the following steps: preparing a bacillus coagulans seed solution, inoculating a soybean meal raw material with the bacillus coagulans seed solution, and carrying out first-stage and second-stage fermentation. The bacillus coagulans strain adopted by the invention still has good adaptability and fermentation capacity in a complex feed matrix environment, and through first-stage fermentation, it is ensured that an initial flora quickly occupies a micro-ecological site in the initial stage of fermentation, so that the growth of infectious microbes is inhibited, the primary degradation reaction of a substrate is accelerated, and the degradation rate of the substrate is increased; in the first stage fermentation, effective degradation of anti-nutritional factors and preliminary improvement of raw material structures are ensured, the second stage fermentation strengthens metabolic conversion of substrates and accumulation of beneficial products and promotes synthesis accumulation of beneficial metabolites such as lactic acid and short-chain fatty acid, and staged fermentation not only realizes deep degradation of the anti-nutritional factors, but also promotes accumulation of the beneficial metabolites, so that the anti-nutritional factors can be effectively degraded. The functionality and the nutritional value of the soybean meal feed are obviously improved.

The present invention discloses a method for rapid detection of eight types of marine Bacillus and its application in fermented foods, belonging to the field of microbial detection and ecological applications. The method of the present invention is used to perform Raman detection on eight strains of marine Bacillus, and a training model is established using the characteristic bands of marine Bacillus to identify unknown strains. The method is applied to Daqu to prepare a Daqu suspension sample by gradient centrifugation, homogenization or vortexing, and Raman detection is performed on single cells. The established marine Bacillus training model is used to perform machine learning on the Raman spectra of unknown bacteria to predict the type of strain, thereby achieving real-time, rapid, and label-free detection of marine Bacillus in Daqu and determining its marine Bacillus type. The detection and analysis time can be shortened to a few minutes.

The invention provides an animal microecological preparation with an anti-stress function and a preparation method thereof, and belongs to the technical field of microecological preparations, and the preparation method comprises the following steps: culturing bacillus subtilis and lactic acid bacteria to obtain a bacillus seed solution and a lactic acid bacteria seed solution, and carrying out aerobic fermentation on the bacillus seed solution to obtain a fermentation liquid; adding a lactic acid bacteria seed solution for micro-aerobic fermentation; culturing saccharomyces cerevisiae to obtain a saccharomyces cerevisiae seed solution, mixing the fermentation product with the saccharomyces cerevisiae seed solution, and sequentially centrifuging and freeze-drying to obtain freeze-dried bacterial powder; the preparation method comprises the following steps: mixing freeze-dried bacterial powder, prebiotics, chlorogenic acid, L-glutamine, yeast beta-glucan and deionized water to obtain a core material solution, mixing the core material solution with a sodium alginate solution, dropwise adding into a calcium chloride solution for crosslinking, soaking in a chitosan solution, soaking in a sodium tripolyphosphate solution again for crosslinking, and carrying out spray drying. Through synergistic fermentation of multiple strains and composite embedding of functional components, the anti-stress function of the animal microecological preparation is improved.

The invention discloses a novel EM (effective microbiota) stock solution and a use method thereof. The stock solution consists of a photosynthetic bacteria group, a lactic acid bacteria group, a saccharomycetes group, an actinomycetes group, a filamentous bacteria group of a fermentation system, bifidobacteria and bacillus. The method can be applied to the fields of cultivation, aquatic products, planting, environmental protection and the like; the organic fertilizer has the characteristics of preventing diseases, promoting growth of animals and plants, improving vitality, shortening the growth cycle and improving the yield in agriculture, and has the effects of inhibiting bacteria, deodorizing and improving the soil environment in the fields of environmental protection and environment; effective strains of the EM stock solution have extremely high deodorization and decomposition capabilities, deodorization work can be completed within 20 seconds, and decomposition work of harmful microorganisms and harmful substances can be achieved within 3 minutes; moreover, the environment resistance is extremely high, 95% of activity (30 minutes) can be kept at the high temperature of 115 DEG C, and the activity is still enough at the low temperature of-27 DEG C.

The present invention relates to the use of a particular bacterial species as a biosolution in the production and cultivation of tomatoes. More particularly, the invention relates to the use of a Bacillus siamensis strain, deposited with the National Collection of Cultures of Microorganisms (CNCM) under the accession number CNCM I-5921, in the stimulation of the development and growth of seeds and of tomato plants or the improvement of the yields of tomato crops, and in the improvement of the resistance and tolerance of seeds, tomato plants and tomato crops to abiotic stress.

The invention belongs to the technical field of biological detection, and discloses primers, a kit and an identification method for identifying bacillus cereus in fermented grains. The primers for identifying the bacillus cereus in the fermented grains are a pair of specific ARMS primers: C3F and CR, and the nucleotide sequences of the primers are respectively shown as SEQ ID NO: 13 and SEQ ID NO: 15. According to different 23sRNA sequences of the bacillus cereus and other related bacillus, primers are designed by adopting a qPCR technology mutation amplification retarding system, and an ARMS-qPCR detection method of the bacillus cereus is established, so that the bacillus cereus in a complex sample can be rapidly and efficiently detected and identified, the experimental period is shortened, and the detection efficiency is improved. Specificity, sensitivity and doping ratio verification shows that the method can be used for rapid and accurate identification, and compared with traditional morphology and physiological and biochemical identification means which have the defects of large error and low efficiency, the method is simple and convenient to operate, rapid in reaction and capable of achieving accurate qualitative identification.

The application provides an animal microecological preparation with stress resistance and a preparation method thereof, and belongs to the technical field of microecological preparations, and comprises the following steps: culturing Bacillus subtilis and lactic acid bacteria to obtain Bacillus subtilis seed liquid and lactic acid bacteria seed liquid, carrying out aerobic fermentation on the Bacillus subtilis seed liquid, and adding the lactic acid bacteria seed liquid to carry out micro-aerobic fermentation; culturing Saccharomyces cerevisiae to obtain yeast seed liquid; after the fermentation product and the yeast seed liquid are mixed, centrifugation and freeze-drying are sequentially carried out to obtain freeze-dried bacterial powder; the freeze-dried bacterial powder, prebiotics, chlorogenic acid, L-glutamine, yeast beta-glucan and deionized water are mixed to obtain a core solution; after the core solution and a sodium alginate solution are mixed, the mixture is dropped into a calcium chloride solution to carry out cross-linking, the mixture is soaked in a chitosan solution, and then the mixture is soaked in a trisodium phosphate solution to carry out cross-linking, and finally, the mixture is subjected to spray drying. Through the composite embedding of multi-strain synergistic fermentation and functional components, the animal microecological preparation is improved in the stress resistance.