Probiotic fermented traditional Chinese medicine composition for preventing and controlling yellow scour of piglets and preparation method of probiotic fermented traditional Chinese medicine composition

Abstract

The invention belongs to the field of veterinary drugs, and particularly relates to a probiotic fermented traditional Chinese medicine composition for preventing and controlling yellow scour of piglets and a preparation method thereof, and the probiotic fermented traditional Chinese medicine composition comprises 100 + / -2 parts of radix sophorae flavescentis, 10 + / -2 parts of fructus forsythiae, 100 + / -2 parts of liquorice, 200 + / -2 parts of dandelion, 100 + / -2 parts of coptis chinensis and 5-20 parts of bacillus velezensis SXDYYB3 preparation. The bacillus velezensis SXDYYB3 preparation is prepared by inoculating a seed solution of the bacillus velezensis SXDYYB3 into a culture medium for fermentation, the bacillus velezensis SXDYYB3 is preserved in the China Center for Type Culture Collection, and the preservation number of the bacillus velezensis SXDYYB3 is CCTCC NO: M 2025428. Two weeks before farrowing of sows, the probiotic fermented traditional Chinese medicine composition for preventing and controlling piglet yellow scour is mixed with feed to feed the sows, and the prevention effect can be achieved; after piglets are born and before yellow scour does not appear, the yellow scour of the piglets can be prevented by feeding the piglets with the medicine mixed with feed, the yellow scour of the piglets can be cured by increasing the dosage after the piglets are attacked, and the prevention and cure rate can reach 70-90%.

Description

Technical Field

[0001] The present invention relates to the technical field of veterinary drugs, and particularly relates to a probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea and a preparation method thereof. Background Art

[0002] Piglets are most prone to yellow diarrhea after birth (also belonging to Escherichia coli). Affected pigs are mostly piglets within 5 days old. At the initial stage of the disease, 1 or 2 pigs suddenly die, and then the whole litter is affected. Diseased pigs are mostly listless, huddled together, excrete yellow pasty loose stools, quickly lose weight, and finally become comatose and die. Piglets suffering from "yellow diarrhea" are thin and reluctant to eat. They initially have diarrhea and then turn to yellow diarrhea. It is contagious. If not treated in time, the piglets will die.

[0003] Chemical antibiotic drugs have preventive and therapeutic effects on piglet yellow diarrhea and are usually the first choice for treating piglet yellow diarrhea. However, Escherichia coli is extremely prone to developing drug resistance to chemical antibiotics such as gentamicin, enrofloxacin, and norfloxacin. Even if treatment or prevention is carried out by alternately using or combining multiple antibacterial drugs in a planned manner, the generation of drug resistance cannot be completely avoided.

[0004] In the field of traditional Chinese medicine, some traditional Chinese medicines have good disease resistance effects. The mechanisms of these traditional Chinese medicines against piglet yellow diarrhea mainly include inhibiting pathogens, antagonizing enterotoxins, inhibiting the production of enterotoxins, and non-specific anti-diarrhea effects.

[0005] Probiotics are live microorganisms that have a beneficial effect on the human body by improving the balance of the intestinal flora, also known as microecological preparations. Their main role is to help establish a gastrointestinal microflora beneficial to the host through the competitive exclusion of organisms in the digestive tract, prevent bacterial and viral diarrhea, and promote growth. Their mechanisms of action mainly include competitive inhibition, enhancing immune function, regulating the balance of the intestinal microecology, improving the intestinal environment, and promoting nutrient absorption.

[0006] Although Chinese herbal medicines or probiotics may improve the intestinal microecological environment of piglets and enhance their immunity, for severe infections that have already occurred, especially piglet yellow diarrhea caused by Escherichia coli, using Chinese herbal medicines or probiotics alone may not achieve the ideal prevention and control effect. Sometimes, before the drug effect takes effect, the piglets die due to severe diarrhea, losing the due effect of the drug.

[0007] Based on this, the present invention is proposed. Summary of the Invention

[0008] The purpose of the present invention is to provide a probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea and a preparation method thereof to solve the above problems.

[0009] The purpose of the present invention is to provide a probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea and a preparation method thereof, which can be achieved through the following technical solutions: In a first aspect, a probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea comprises raw materials in the following parts by mass: 100±2 parts of Sophora flavescens, 10±2 parts of Forsythia suspensa, 50 - 100 parts of Glycyrrhiza uralensis, 100 - 200 parts of Taraxacum mongolicum, 50 - 100 parts of Coptis chinensis, and 5 - 20 parts of Bacillus velezensis SXDYYB3 preparation; The Bacillus velezensis SXDYYB3 preparation is prepared by inoculating a Bacillus velezensis SXDYYB3 seed solution into a culture medium for fermentation. Bacillus velezensis SXDYYB3 was deposited at the China Center for Type Culture Collection on March 10, 2025, with the deposit number CCTCC NO: M 2025428.

[0010] Furthermore, the probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea comprises raw materials in the following parts by mass: 100 parts of Sophora flavescens, 10 parts of Forsythia suspensa, 100 parts of Glycyrrhiza uralensis, 200 parts of Taraxacum mongolicum, 100 parts of Coptis chinensis, and 10 parts of Bacillus velezensis SXDYYB3 preparation.

[0011] Furthermore, the fermentation conditions of the Bacillus velezensis SXDYYB3 seed solution in the culture medium are as follows: after continuous culturing at 35°C for 48 hours, static culturing for 96 hours, and the bacterial count is greater than 1.80×10 9 CFU / mL.

[0012] Furthermore, the culture medium is composed of the following components by weight: 3±0.5 parts of peptone, 2±0.5 parts of beef extract, 7±1 part of yeast extract, 11±1 part of sodium chloride, 75±5 parts of fructooligosaccharide, and 1000±50 parts of water.

[0013] Furthermore, the probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea further comprises pharmaceutically acceptable medicinal excipients.

[0014] Furthermore, the medicinal excipients are pharmaceutically acceptable carriers, diluents, excipients, and flavoring agents.

[0015] In a second aspect, a preparation method of the probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea comprises the following steps: Step 1: Weigh Sophora flavescens, Forsythia suspensa, Glycyrrhiza uralensis, Taraxacum mongolicum, and Coptis chinensis, crush them, add water, boil and decoct to obtain a first medicinal liquid; Step 2: After cooling the first medicinal liquid, add the Bacillus velezensis SXDYYB3 preparation to obtain the product.

[0016] Furthermore, in Step 1, after crushing, it is sieved through an 80-mesh sieve.

[0017] Further, in step 1, add water 10 times the total weight of Sophora flavescens, Forsythia suspensa, Glycyrrhiza uralensis, Taraxacum mongolicum, and Coptis chinensis, and boil for 30 minutes.

[0018] Thirdly, piglet yellow diarrhea is mainly caused by dysbacteriosis. The probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea needs to emphasize the synergy of "microecological regulation + pathogen inhibition" more. The preparation of Bacillus velezensis SXDYYB3 is used as the synergistic monarch drug, and together with Coptis chinensis, it dominates the treatment direction, and its application in the preparation of drugs for preventing and / or treating piglet yellow diarrhea according to the four dimensions of "antibacterial - anti-inflammatory - mucosal protection - microecological regulation".

[0019] Meaning and explanation of the formula: 1. Core medicine pair (combined with monarch drug): Coptis chinensis + preparation of Bacillus velezensis SXDYYB2 Coptis chinensis: Clears heat and dries dampness, targets and inhibits ETEC (berberine blocks bacterial adhesion); Bacillus velezensis SXDYYB2: Competes for occupancy, secretes antibacterial peptides (velezicin directly lyses pathogenic bacteria).

[0020] 2. Antibacterial and anti-inflammatory module (ministerial drug): Forsythia suspensa + Taraxacum mongolicum Forsythia suspensa: Clears heat and detoxifies (forsythoside inhibits the release of bacterial endotoxin); Taraxacum mongolicum: Promotes diuresis and relieves stranguria (chlorogenic acid reduces intestinal oxidative stress).

[0021] 3. Mucosal repair module (adjuvant drug): Glycyrrhiza uralensis Glycyrrhiza uralensis: Glycyrrhizic acid promotes the secretion of mucin (repairs the intestinal barrier), and neutralizes the bitter cold property of Coptis chinensis (prevents piglets from having stomach cold and anorexia).

[0022] 4. Microecological regulation module (conducting drug): Sophora flavescens + preparation of Bacillus velezensis SXDYYB2 Sophora flavescens: Matrine selectively inhibits pathogenic bacteria (does not affect probiotics) and synergizes with SXDYYB2 to optimize the flora structure.

[0023] Compared with the prior art, the beneficial effects of the present invention are: 1. Two weeks before farrowing, feed the sow with the probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea mixed with feed, which can play a preventive role; after the piglets are born and before yellow diarrhea appears, feed the piglets with this medicine mixed in the feed to prevent piglet yellow diarrhea. After the disease occurs, increase the dosage to cure it, and the prevention and cure rates can both reach 70 - 90%.

[0024] 2. In the prior art, although traditional Chinese medicines such as Sophora flavescens and Coptis chinensis have antibacterial effects, the cure rate for piglet yellow diarrhea when used alone is less than 50%. Conventional probiotics (such as lactic acid bacteria) have low survival rates in the gastric acid environment and are difficult to effectively colonize the intestinal tract. In the present invention, a traditional Chinese medicine composition is fermented by a specific probiotic (Bacillus velezensis SXDYYB3), which increases the conversion rate of pharmacodynamic components by more than 40%. At the same time, the colonization rate of the probiotic in the intestinal tract reaches 85%, and they play a synergistic therapeutic role.

[0025] 3. The bacterial count of Bacillus velezensis SXDYYB3 in the Bacillus velezensis SXDYYB3 preparation is greater than 1.80×10 9 CFU / mL. Bacillus velezensis SXDYYB3 has strong activities such as protease, lipase, and amylase, can produce antibiotics, has strong biological oxygen scavenging ability in the animal intestinal tract, and can produce L-lactic acid under anaerobic conditions. These characteristics play an important role in promoting the digestion and absorption of animal nutrition, improving the feed conversion rate of animals, preventing diseases, and promoting growth.

[0026] 4. The present invention is particularly suitable for the prevention and treatment of Escherichia coli diarrhea in piglets within 5 days of age. Detailed implementation mode

[0027] The following further describes the present invention in detail through specific examples.

[0028] The following detailed description of the embodiments of the present application is not intended to limit the scope of the present application that is claimed, but merely represents selected embodiments of the present application. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present application without making creative efforts fall within the scope of protection of the present application.

[0029] Example 1 Strain screening Bacillus velezensis SXDYYB3 was isolated from the intestinal tract of healthy piglets and identified as Bacillus velezensis by 16S RNA. It was deposited in the China Center for Type Culture Collection, and the deposit number is CCTCC NO: M 2025428. The specific method is as follows: Take the jejunum contents of healthy piglets, dilute them with sterile PBS, kill non-spore bacteria by water bath at 80°C for 10 min, and coat them on an LB plate (pH 6.5) containing 1% konjac powder, and anaerobically culture at 37°C for 48 hours.

[0030] Screening criteria: Select colonies with a hydrolysis zone diameter / colony diameter ≥ 2.5.

[0031] Identification result: It was identified as Bacillus velezensis by 16S rRNA sequencing, and its characteristics are as follows: It produces β-mannanase activity (320 ± 15 U / mL); it has acid resistance (survival rate > 90% in an environment with pH 2.5); it has antibacterial properties (the diameter of the antibacterial zone against ETEC K88 is 18 ± 1 mm).

[0032] Example 2 Formula 1: 100 g of Sophora flavescens, 10 g of Forsythia suspensa, 100 g of Glycyrrhiza uralensis, 200 g of Taraxacum mongolicum, 100 g of Coptis chinensis, and 10 g of Bacillus velezensis SXDYYB3 preparation.

[0033] Preparation method, including the following steps: 1). Weigh the raw medicinal materials Sophora flavescens, Forsythia suspensa, Glycyrrhiza uralensis, Taraxacum mongolicum, and Coptis chinensis according to the above formula, crush them (pass through an 80-mesh sieve), add 10 times the total weight of the medicinal materials of normal temperature water, and boil for 30 minutes to obtain the first medicinal liquid; 2). After cooling the first medicinal liquid, add the Bacillus velezensis SXDYYB3 preparation to obtain Traditional Chinese Medicine Composition 1.

[0034] The Bacillus velezensis SXDYYB3 preparation is prepared by inoculating the Bacillus velezensis SXDYYB3 seed liquid into a culture medium for fermentation; the fermentation conditions are as follows: After continuous culture at 35 °C for 48 hours, static culture for 96 hours, and the number of bacteria is greater than 1.80×10 9 CFU / mL; the culture medium is composed of the following components by weight: 3 g of peptone, 2 g of beef extract, 7 g of yeast extract (purchased from Guangheng Food Ingredients Co., Ltd., Henan Province), 11 g of sodium chloride, 75 g of fructooligosaccharide, and 1000 g of water.

[0035] Therapeutic effect verification test: Animal model: Select 60 3-day-old piglets infected with ETEC K88 and randomly divide them into 3 groups Drug administration plan: Experimental group: Traditional Chinese Medicine Composition 1 (containing 10 9 CFU / mL Bacillus velezensis SXDYYB3) Control group 1: Unfermented traditional Chinese medicine composition (compared with Traditional Chinese Medicine Composition 1, it does not contain 10 9 CFU / mL Bacillus velezensis SXDYYB3) Control group 2: Enrofloxacin.

[0036] The test results are shown in Table 1: Table 1

[0037] The measurement of the 24-hour antidiarrheal rate is to evaluate the changes in the number of stools, stool characteristics, and related symptoms before and after treatment. The specific method is as follows: Definition of diarrhea standard: Diarrhea is usually defined as a significant increase in the number of bowel movements within 24 hours (e.g., ≥3 times), accompanied by abnormal stool characteristics (e.g., loose stools, watery stools, mucous stools, or bloody stools).

[0038] Basis for calculating the antidiarrheal rate: The calculation of the antidiarrheal rate is usually based on the improvement of diarrhea symptoms after treatment. For example: After 24 hours of treatment, the number of daily bowel movements is reduced to ≤3 times, the stool characteristics are close to normal, and the clinical symptoms disappear.

[0039] Intestinal sIgA value: The intestinal secretory immunoglobulin A (sIgA) was measured by enzyme-linked immunosorbent assay (ELISA).

[0040] As can be seen from Table 1, the treatment effect of the experimental group is better than that of the unfermented traditional Chinese medicine in Control Group 1 and enrofloxacin in Control Group 2.

[0041] The 24-hour antidiarrheal rate of the experimental group (85%) was significantly higher than that of Control Group 1 (38%) treated with traditional Chinese medicine alone and the antibiotic group (72%) (p<0.01), and the intestinal sIgA level (32.5±2.1 μg / mL) reached 1.7 - 2.6 times that of the control group, indicating that traditional Chinese medicine and SXDYYB3 synergize through the following pathways: traditional Chinese medicine clears pathogenic bacteria and optimizes the colonization environment of probiotics; probiotics enhance the intestinal immune response and amplify the anti-inflammatory effect of traditional Chinese medicine.

[0042] Example 3 It was confirmed by HPLC detection that compared with the unfermented traditional Chinese medicine composition, the content of matrine-berberine complex in the fermented traditional Chinese medicine composition 1 increased by 2.3 times; the results are shown in Table 2: Table 2

[0043] Verification of the necessity of the fermentation process Fermentation vs unfermented: Diarrhea inhibition rate: 89.3% vs 52.1% (P<0.01); Recovery of pepsin activity: 85% vs 40%; The fermentation effects of different strains are shown in Table 3: Table 3

[0044] In Table 3, Bacillus subtilis vulgaris was purchased from Cangzhou Dongfang Veterinary Medicine Co., Ltd., and lactic acid bacteria were purchased from Shandong Xinjiang Chemical Industry Co., Ltd.

[0045] The detection method of enterotoxigenic Escherichia coli (ETEC) uses multiplex PCR method.

[0046] Example 4 Formula 2: 100 g of Sophora flavescens, 10 g of Forsythia suspensa, 100 g of Glycyrrhiza uralensis, 200 g of Taraxacum mongolicum, 100 g of Coptis chinensis, 20 g of Bacillus velezensis SXDYYB3 preparation.

[0047] The preparation method includes the following steps: 1), Weigh the crude drugs Sophora flavescens, Forsythia suspensa, Glycyrrhiza uralensis, Taraxacum mongolicum and Coptis chinensis according to the above formula, pulverize them (pass through an 80-mesh sieve), add 10 times the total weight of the herbs of normal temperature water and boil for 30 minutes to obtain the first medicinal liquid; 2), After cooling the first medicinal liquid, add the Bacillus velezensis SXDYYB3 preparation to obtain the traditional Chinese medicine composition 2.

[0048] The Bacillus velezensis SXDYYB3 preparation is prepared by inoculating the Bacillus velezensis SXDYYB3 seed liquid into a culture medium for fermentation; the fermentation conditions are: continuously culturing at 35 °C for 48 hours, then statically culturing for 96 hours, and the bacteria count is greater than 1.80×10 10 CFU / mL; the culture medium is composed of the following components by weight: 3 g of peptone, 2 g of beef extract, 7 g of yeast extract (such as purchased from Henan Guangheng Food Ingredients Co., Ltd.), 11 g of sodium chloride, 75 g of fructooligosaccharide, and 1000 g of water.

[0049] Example 5 Formula 3: 100 g of Sophora flavescens, 10 g of Forsythia suspensa, 50 g of Glycyrrhiza uralensis, 100 g of Taraxacum mongolicum, 50 g of Coptis chinensis, 5 g of Bacillus velezensis SXDYYB3 preparation.

[0050] The preparation method includes the following steps: 1), Weigh the crude drugs Sophora flavescens, Forsythia suspensa, Glycyrrhiza uralensis, Taraxacum mongolicum and Coptis chinensis according to the above formula, pulverize them (pass through an 80-mesh sieve), add 10 times the total weight of the herbs of normal temperature water and boil for 30 minutes to obtain the first medicinal liquid; 2), After cooling the first medicinal liquid, add the Bacillus velezensis SXDYYB3 preparation to obtain the traditional Chinese medicine composition 3.

[0051] The Bacillus velezensis SXDYYB3 preparation is prepared by inoculating the Bacillus velezensis SXDYYB3 seed liquid into a culture medium for fermentation; the fermentation conditions are: continuously culturing at 35 °C for 48 hours, then statically culturing for 96 hours, and the bacteria count is greater than 2×10 9CFU / mL; The culture medium consists of the following components by weight: 3 g of peptone, 2 g of beef extract, 7 g of yeast extract (such as purchased from Guangheng Food Ingredients Co., Ltd., Henan Province), 11 g of sodium chloride, 75 g of fructooligosaccharide, and 1000 g of water.

[0052] Example 6 Formula 4: 100 g of Sophora flavescens, 10 g of Forsythia suspensa, 66.7 g of Glycyrrhiza uralensis, 100 g of Taraxacum mongolicum, 100 g of Coptis chinensis, and 10 g of Bacillus velezensis SXDYYB3 preparation.

[0053] The preparation method is the same as that of Example 5.

[0054] The usage method of the probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea of the present invention: Two weeks before the sow gives birth, mix this medicine with feed and feed the sow, which can play a preventive role; after the piglets are born and before yellow diarrhea appears, mix this medicine with feed and feed the piglets to prevent piglet yellow diarrhea. After the disease occurs, increase the dosage of the medicine to achieve a complete cure. The following are some application examples: 1. Use the traditional Chinese medicine composition 1 of Example 2. Mix 300 g of the traditional Chinese medicine composition 1 with 3000 g of feed per meal and feed the sows about to give birth for 3 days. The piglets did not develop yellow diarrhea after birth.

[0055] 2. Use the traditional Chinese medicine composition 2 of Example 4. Mix 100 g of the traditional Chinese medicine composition 2 with 1000 g of feed per meal per piglet and feed the piglets with "yellow diarrhea" for 3 consecutive days. The one-time cure rate reaches 70%; after a 7-day interval, for another course of treatment, and for 3 consecutive courses, the cure rate can reach 90%.

[0056] 3. Use the traditional Chinese medicine composition 3 of Example 5. Mix 20 g of the traditional Chinese medicine composition 3 into the feed per piglet and feed continuously for 2 days for prevention. More than 70% of the piglets prevented from getting yellow diarrhea when they were sold. Other piglets not treated with prevention suffered from yellow diarrhea; the latter were fed in the same way for one week and were cured.

[0057] Although the preferred embodiments of the present invention have been described, those skilled in the art can make additional changes and modifications once they know the basic creative concepts. Therefore, the appended claims are intended to be construed to include the preferred embodiments as well as all changes and modifications falling within the scope of the present invention.

[0058] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention is also intended to include these modifications and variations.

Claims

1. A probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea, characterized in that: Comprising the following raw materials in parts by mass: 100±2 parts of Sophora flavescens, 10±2 parts of Forsythia suspensa, 50-100 parts of Glycyrrhiza uralensis, 100-200 parts of Taraxacum mongolicum, 50-100 parts of Coptis chinensis, 5-20 parts of Bacillus velezensis SXDYYB3 preparation; The Bacillus velezensis SXDYYB3 preparation is prepared by inoculating the Bacillus velezensis SXDYYB3 seed liquid into a culture medium for fermentation. Bacillus velezensis SXDYYB3 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2025428.

2. The probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea according to claim 1, wherein: Comprising the following raw materials in parts by mass: 100 parts of Sophora flavescens, 10 parts of Forsythia suspensa, 100 parts of Glycyrrhiza uralensis, 200 parts of Taraxacum mongolicum, 100 parts of Coptis chinensis, 10 parts of Bacillus velezensis SXDYYB3 preparation.

3. The probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea according to claim 1, characterized in that: The fermentation conditions of the Bacillus velezensis SXDYYB3 seed liquid in the medium are as follows: after continuous cultivation at 35 °C for 48 hours, static cultivation for 96 hours, and the number of bacteria is greater than 1.80×10 9 CFU / mL.

4. The probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea according to claim 3, wherein: The culture medium is composed of the following components by weight: 3±0.5 parts of peptone, 2±0.5 parts of beef extract, 7±1 part of yeast extract, 11±1 part of sodium chloride, 75±5 parts of fructooligosaccharide, 1000±50 parts of water.

5. The probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow dysentery according to claim 1, wherein: It also includes pharmaceutically acceptable pharmaceutical excipients.

6. The probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea according to claim 5, wherein: The pharmaceutical excipients are pharmaceutically acceptable carriers, diluents, excipients, and flavoring agents.

7. The preparation method of the probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea as described in claim 1, wherein: Comprising the following steps: Step 1: Weigh Sophora flavescens, Forsythia suspensa, Glycyrrhiza uralensis, Taraxacum mongolicum, and Coptis chinensis, crush them, add water and boil and decoct to obtain the first medicinal liquid; Step 2: Add the Bacillus velezensis SXDYYB3 preparation after cooling the first medicinal liquid to obtain the product.

8. The preparation method of the probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea according to claim 7, characterized in that: In Step 1, after crushing, it is sieved through an 80-mesh sieve.

9. The preparation method of the probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea according to claim 7, wherein: In Step 1, add 10 times the total weight of Sophora flavescens, Forsythia suspensa, Glycyrrhiza uralensis, Taraxacum mongolicum, and Coptis chinensis of water and boil for 30 minutes.

10. Use of the probiotic-fermented traditional Chinese medicine composition for preventing and controlling piglet yellow diarrhea according to any one of claims 1-6 in the preparation of a drug for preventing and / or treating piglet yellow diarrhea.

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