104 results about "Genus Pseudomonas" patented technology

The invention discloses a method for denitrifying wastewater by using a low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacterium, and relates to a method for denitrifying wastewater by using a biological bacterium, the bacterium is Pseudomonas putida, is named as Pseudomonas umsongensis BK17, belongs to Pseudomonas, and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024. The strain is a heterotrophic nitrification-aerobic denitrification bacterium, can efficiently remove inorganic nitrogen pollution in a water body, still has good denitrification performance under low-temperature stress, and is high in low-temperature tolerance. The strain can efficiently treat nitrogen-containing wastewater under the condition of 10 DEG C, and the removal efficiency can reach 99% or above. The method has great potential in the application of inorganic nitrogen pollution treatment of domestic sewage and other water bodies in cold regions in China.

The invention discloses a high-efficiency lespedeza growth-promoting bacillus Q4 (Bacillus atrophaeus) capable of improving the abundance of dominant bacteria and a preparation method of the high-efficiency lespedeza growth-promoting bacillus Q4. Belongs to the technical field of microorganisms. According to the bacillus atrophaeus Q4 provided by the invention, the conversion from total-effect nutrients to effective nutrients is improved, the consumption of lespedeza to soil nutrients is enhanced, and the abundance of dominant strain groups is increased, so that the effect of promoting the growth of lespedeza is achieved. After the bacillus atrophaeus Q4 provided by the invention is applied, the soil enzyme activity is remarkably improved, the conversion of available nutrients in soil is remarkably enhanced, and the nutrient consumption capability of lespedeza is enhanced. By applying the bacillus atrophaeus Q4, growth and development of lespedeza are promoted, the content of soil hydrolytic nitrogen and available phosphorus is remarkably increased, conversion to soil nutrients is improved, and the conductivity and organic carbon are increased to the maximum extent and are remarkably increased by 142.77% and 64.26% respectively. In the genus level, after being treated by the bacillus atrophaeus Q4, the bacillus (Bacillus) is obviously increased from 0.84% to 2.47%, and the average relative abundance of the pseudomonas (Fictibacillus) is obviously increased from 0.59% to 1.16%. By inoculating the Q4 inoculant, the structure of a soil colony is greatly improved, the relative abundance of a dominant flora is influenced, and the obvious increase of the relative abundance of Bacillus and Fictibacillus is possibly an important factor for promoting plant growth.

The invention provides an L-threonine aldolase mutant and application of the L-threonine aldolase mutant in catalytic synthesis of p-methylsulfonylphenylserine, the L-threonine aldolase mutant is derived from pseudomonas, threonine and p-methylsulfonylbenzaldehyde can be used as substrates, and the L-threonine aldolase mutant can be synthesized by using a one-step method. According to the present invention, L-threonine aldolase is adopted as a raw material, pyridoxal phosphate and reduced nicotinamide adenine dinucleotide are adopted as coenzymes, the synthesis of florfenicol intermediate product p-methylsulfonyl phenyl serine is obtained through catalysis, and compared with the wild type L-threonine aldolase, the mutant enzyme has high activity, can effectively improve the conversion efficiency, and can shorten the conversion time; the enzyme and the technical method provided by the invention have the advantages of mild reaction conditions and less pollution, and have good industrial prospects in the production of florfenicol synthesis intermediates.

The invention provides a growth-promoting rhizobacteria for enhancing salt tolerance of corn and application of the growth-promoting rhizobacteria, and belongs to the technical field of agricultural microorganisms. The invention provides a pseudomonas bacterium TJ1-185, and the preservation number of the pseudomonas bacterium TJ1-185 is CDMCC (China Description of Microbiological Culture Collection Center) No.64979. The pseudomonas bacteria TJ1-185 can improve the salt stress resistance of plants and relieve the damage of salt stress to the plants. The result of the embodiment shows that the pseudomonas bacterium TJ1-185 can promote corn to germinate under salt stress, improve the plant height, root length, overground part fresh weight, root fresh weight, overground part dry weight and root dry weight of corn under the salt stress condition, and reduce the malondialdehyde content, thereby promoting the growth of corn under the salt stress condition and improving the salt stress resistance of corn. The damage of salt stress to the corn is relieved.

The invention belongs to the field of microbiological detection, and discloses a rapid detection chromogenic culture medium for pseudomonas, and the chromogenic detection culture medium comprises a chromogenic substrate, an endogenous enzyme promoter, a basic culture medium and a bacteriostatic agent. Specifically, the pseudomonas can react with a chromogenic substrate, so that a bacterial colony is blue-green, and other non-pseudomonas does not grow or weakly grow under the action of a bacteriostatic agent, or the chromogenic substrate different from the pseudomonas is utilized to display purple red or other colors which are inconsistent with the color of the pseudomonas bacteria, so that the pseudomonas bacteria can be distinguished, and the pseudomonas bacteria can be distinguished. And rapid separation and identification are realized. According to the rapid detection chromogenic culture medium for the pseudomonas, the glycoside chromogenic substrate and the endogenous enzyme accelerant are utilized, the problem that when the rapid detection chromogenic culture medium for the pseudomonas is prepared, the pseudomonas has chromatic aberration or does not have color development is solved, and the blank of the rapid detection chromogenic culture medium for the pseudomonas in the domestic market is filled.

The present invention relates to an endophyte strain isolated from a plant of the Poaceae family, wherein said endophyte is a strain of Pseudomonas poae which provides bioprotection and / or biofertilizer phenotypes to plants into which it is inoculated. The present invention also relates to plants infected with the endophyte and related methods.

Exemplary embodiments of the present disclosure are directed towards a carrier-based agricultural biofertilizer composition for enhanced disease management in legumes includes, an isolated fluorescens strain CGA FPBPF-034 having the deposit accession number KY495220, and an agriculturally acceptable solid or liquid formulation with an organic or inorganic stabilizer to provide stability and support for the Pseudomonas species, thereby enhancing the disease management efficacy and overall health of the plants.

The invention discloses pseudomonas capable of dissolving phosphorus and producing siderophores and application of the pseudomonas, and relates to the technical field of microorganisms. The bacterial strain P-14-21 is identified as a new species of Pseudomonas sp. And is named as Pseudomonas sp. P-14-21, the bacterial strain is preserved in Guangdong Microbial Culture Collection Center on August 7, 2024, and the preservation number is GDMCC No: 64969. The invention also discloses a preparation method of the Pseudomonas sp. P-14-21, and the bacterial strain is named as Pseudomonas sp. P-14-21. The bacterial strain is named as Pseudomonas sp. P-14-21 and is preserved in Guangdong Microbial Culture Collection Center on August 7, 2024. Besides, the pseudomonas P-14-21 disclosed by the invention has the capacity of dissolving organic phosphorus and high-yield siderophore, the phosphorus dissolving index can reach 2.4, and the activity unit (su) of the siderophore can reach 94.41%, so that the strain has excellent development and application values in agricultural production.

The invention discloses preparation of a microbial compound element-based fertilizer and application of the microbial compound element-based fertilizer in saline-alkali soil treatment, and relates to the technical field of saline-alkali soil improvement. The preparation method comprises the following steps: inoculating lactic acid bacteria, rhodopseudomonas, phosphate-solubilizing bacillus, nitrogen-fixing bacillus, halophilic bacillus and halomonas into a composite culture medium in proportion, and carrying out stepped sequential fermentation culture under the conditions that the pH value is 7.5 and the temperature is 28 DEG C until the bacterial count is greater than or equal to 1010 CFU / mL. According to the preparation of the fertilizer based on the microbial compound elements and the application of saline-alkali soil treatment, a synergistic system of the compound functional flora, the chelated mineral substances and the intelligent organic carrier is constructed, so that multi-dimensional improvement of saline-alkali soil is realized, the limitation of a traditional single improvement mode is broken through, the salinity and pH value of the soil are effectively reduced, and the soil quality is improved. The soil fertility and biological activity are remarkably improved, the salt stress resistance of crops is enhanced, the effective period is prolonged, and the preparation cost is low.

The invention relates to the technical field of paper cups, and discloses a compound bacterium for degrading nitrite in an aquaculture water body, the compound bacterium is composed of nitrifying bacteria, denitrifying bacteria, bacillus coagulans, a penetrant and a synergist, the nitrifying bacteria are mixed flora of autotrophic ammonia oxidizing bacteria and heterotrophic nitrifying bacteria, and the penetrant is a mixture of bacillus coagulans, denitrifying bacteria, bacillus coagulans, a penetrant and a synergist. The denitrifying bacteria comprise a synergistic effect system of the Pseudomonas (Pseudomonas) and the Paracoccus (Paracoccus), and the Pseudomonas (Pseudomonas) and the Paracoccus (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas)) are The dual-path directional conversion of nitrite is realized through the synergistic effect of constructed nitrification-denitrification synergistic flora and a functional aid (polyethylene glycol-SDS-L-glutamine system). Parameter control shows that in a water body (NO2 <-> is equal to 2.5 mg / L) in the later culture period of the penaeus vannamei boone, the degradation rate reaches 81.4% within 48 hours, and the nitrate increment is controlled within 12%. The technology breaks through the bottleneck of failure of a traditional microbial agent in a high-salinity (35 per mill) and high-density culture environment, and is suitable for sea and fresh water whole-variety aquaculture.

The invention belongs to the technical field of microorganisms, and particularly relates to a pseudomonas TaRb6 capable of preventing diseases and promoting growth and application of the pseudomonas TaRb6. The TaRb6 strain provided by the invention belongs to a potential new species of pseudomonas, and the preservation number of the TaRb6 strain is CGMCC (China General Microbiological Culture Collection Center) No.36131. The strain TaRb6 and a fermentation inoculant prepared from the strain TaRb6 have good disease prevention and growth promotion functions on plants; the bacterial inhibition rate of sterile fermentation liquor of the strain TaRb6 on fusarium pseudograminearum reaches 46.54%-62.82%, and the bacterial colony diameter of the fusarium pseudograminearum can be reduced by 61.38% through metabolized volatile organic compounds of the strain TaRb6. After the TaRb6 bacterial suspension is used for seed soaking treatment of wheat, the incidence rate of wheat basal stem rot can be remarkably reduced, and the growth vigor of wheat can be promoted. In addition, the strain TaRb6 has broad-spectrum antibacterial ability, and the antibacterial rate of the strain TaRb6 on pathogenic bacteria of fusarium, botrytis cinerea, apical cyst and xanthomonas reaches 47.23%-67.02%.

Modified bacterial strain of the Pseudomonas sp. genus, the strain of Pseudomonas stutzeri deposited under Deposit Accession No. RGM3505 and a modified bacterial strain of the Bacillus sp. genus, a strain of Bacillus velezensis deposited under Accession No. RGM3504. The strains may be combined at a Pseudomonas stutzeri to Bacillus velezensis ratio from 1 :10 to 10:1 CFU / mL. The strains were modified to have greater chemotactic attraction towards plant roots and to improve the beneficial features of crops, for example, of legumes.

The invention provides a method for improving tetrahydrofolic acid biosynthesis based on NADPH cofactor circulation, and aims to catalyze folic acid into tetrahydrofolic acid through an in-vitro enzyme catalysis technology. According to the invention, escherichia coli BL21 (DE3) is used as a host cell, and dihydrofolate reductase (SmDHFR) derived from serratia marcescens and formate dehydrogenase (pseudoFDH) derived from pseudomonas, which are obtained by screening, are expressed. A TB culture medium is used for fermentation, ultrasonic cell disruption and purification to obtain pure enzyme liquid of SmDHFR and pseFDH. SmDHFR is responsible for converting folic acid into tetrahydrofolic acid, and NADPH consumed in the process is converted into NADP < + >. And the pseudoFDH is used for reducing the generated NADP < + > into NADPH so as to realize the cyclic utilization of the NADPH cofactor. By exploring the optimal ratio of the addition concentrations of the two enzymes, the optimal reaction pH and the optimization of the substrate concentration, the final yield of tetrahydrofolic acid is 3661.14 mu M.

According to the invention, four specific strains are obtained by separating, purifying and culturing from rhizosphere soil of angelica sinensis seedlings, and are identified as bacillus and pseudomonas by virtue of microbe 16S rDNA (ribosomal deoxyribonucleic acid) sequencing, and the four specific strains are respectively bacillus subtilis NF6, Pseudomonas koreensis ISP16, Pseudomonas germanica IAA17 and Pseudomonas alli KPS68. The strain is preserved in China Center for Type Culture Collection in Wuhan University Life Science College, Wuhan, Hubei, China; the strain is preserved in January 15, 2025, the postcode of the strain is 430072, the preservation date of the strain is January 15, 2025, and the preservation numbers of the strain are respectively CCTCC M 2025155, CCTCC M 2025156, CCTCC M 2025157 and CCTCC M 2025158. The strain obtained by the invention can be used for preparing a green, environment-friendly, efficient, low-toxicity and high-cost-performance microbial agent for promoting growth of angelica sinensis and preventing and treating early bolting of angelica sinensis, has important environmental protection significance and economic value for increasing the yield of angelica sinensis, and is worthy of popularization and application.

To provide a detection method capable of rapidly detecting drug-resistant bacteria, bacteria of the genus Staphylococcus, and bacteria of the genus Pseudomonas; a method for providing an index for selecting an antimicrobial agent for bacteria; and a primer set usable in these methods.SOLUTION: The detection method for drug-resistant bacteria, bacteria of the genus Staphylococcus, and bacteria of the genus Pseudomonas according to the present disclosure comprises a step of performing nucleic acid amplification of target genes (1) to (3) and (4) below for a target sample, and detecting drug-resistant bacteria, bacteria of the genus Staphylococcus, and bacteria of the genus Pseudomonas in the sample: (1) the mecA gene; (2) the spa gene of Staphylococcus aureus; (3) the tuf gene and / or the rpoB gene of bacteria of the genus Staphylococcus; and (4) the 16S rRNA gene of bacteria of the genus Pseudomonas.SELECTED DRAWING: Figure 1

The invention discloses a component optimization method for preparing a liquid fertilizer from acetylisovaleryltylosin tartrate waste liquid, and relates to the technical field of fertilizer preparation, and the component optimization method comprises the following specific steps: step 1, selecting nanoscale activated carbon with the specific surface area of 1000m < 2 > / g-1500m < 2 > / g as a base material, the method has the advantages that a microbial flora system is constructed by screening out pseudomonas bacteria, actinomycetes and bacillus, and the pseudomonas bacteria can effectively decompose complex organic pollutants in the waste liquid by virtue of the mineralization effect of the pseudomonas bacteria and gradually convert the complex organic pollutants into carbon dioxide, water and simple organic acid, so that the primary purification of the waste liquid is realized; actinomycetes can utilize secreted enzymes to convert acetylisovaleryltylosin tartrate into small molecule compounds containing nitrogen, phosphorus, potassium and other nutrient elements, bacillus further promotes synthesis and stability of the nutrient small molecules, it is ensured that the liquid fertilizer is comprehensive in nutrient and accurate in content, the plant growth requirement can be better met, and the liquid fertilizer has a good application prospect. The resource utilization rate of the acetylisovaleryltylosin tartrate waste liquid is greatly improved.

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

The application provides a method for separating and purifying 3-hydroxydecanoic acid from Pseudomonas bacteria. First, the fermentation product of Pseudomonas bacteria is decomposed by alkali to obtain monomers of 3-hydroxydecanoic acid. Then, activated carbon is used for decolorization, and microfiltration membrane and ultrafiltration membrane are used for treatment to remove macromolecular impurities. Finally, a sodium 3-hydroxydecanoate solution is loaded onto a polystyrene-divinylbenzene macroporous resin column modified by polyethyleneimine, and eluted with an ethanol-water (volume ratio 1:1) solution with a pH of 11. The eluate is collected, ethanol is evaporated, a purified sodium 3-hydroxydecanoate solution is obtained, hydrochloric acid is added to adjust the pH to 3-4, and the precipitate is collected by centrifugation and dried to obtain a 3-hydroxydecanoic acid product. The content of 3-hydroxydecanoic acid is detected by liquid chromatography, and the total content of 3-hydroxydecanoic acid in the purified product is higher than 90%. The method uses less organic solvent, the steps are simple, the purity of the obtained product is high, and the application requirements of cosmetics and pharmaceutical intermediates are met.

The present disclosure provides methods of diagnosing and treating Pseudomonas infections in a subject in need thereof based on the mutational signature (e.g., DNA mismatch repair- deficit (dMMR) mutational signature) of the Pseudomonas bacteria. These methods are based on detecting DNA mismatch repair-deficit (dMMR) mutational signature of Pseudomonas species.

A low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacteria is used for wastewater denitrification method, relates to a kind of biological bacteria for wastewater denitrification method, the bacteria is Pseudomonas umsongensis BK17, belongs to Pseudomonas, is preserved in China typical culture preservation center, and the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024.The strain is a heterotrophic nitrification-aerobic denitrification bacteria, can efficiently remove inorganic nitrogen pollution in water body, and still has good denitrification performance under low temperature stress, and has strong low temperature tolerance.The strain can efficiently treat nitrogen-containing wastewater at 10 DEG C, and the removal efficiency can reach more than 99%.In the inorganic nitrogen pollution treatment and application of various water bodies such as domestic sewage in cold regions of China, it has great potential.

Provided are bispecific antibodies that specifically recognize PcrV and / or Psl from Pseudomonas aeruginosa, pharmaceutical compositions comprising antibodies specifically recognizing non-overlapping epitopes of Pseudomonas PcrV and / or Psl. Also provided are methods of making and using these antibodies.

This invention discloses a method for coupling physicochemical treatment and biodegradation of plastics. The method includes the following steps: S1. Mixing plastics, organic solvents, and a metal catalyst, and reacting to degrade them to obtain organic products. The metal catalyst is cobalt acetate and / or manganese acetate; S2. Biodegrading the organic products from step S1 using a mixed colony, which includes *Pseudomonas*, *Bacillus*, *Flavobacterium*, and *Rhodococcus*. This invention uses cobalt acetate and / or manganese acetate as metal catalysts. The catalytic oxidation of the metal catalyst can efficiently depolymerize traditional plastics, overcoming the bottleneck of C-C bond breaking that is difficult for microorganisms to achieve. It can successfully convert plastics into organic products mainly composed of dicarboxylic acids, benzoic acid, and terephthalic acid, and the mixed colony process completely mineralizes the organic products. This method achieves complete removal of plastics without producing any toxic or harmful substances.

The invention relates to a Pseudomonas sp. strain for use in the production of a recombinant protein characterised in that said strain comprises a nucleotide sequence encoding a phi15 RNA polymerase. The invention further relates to a plasmid, capable of integrating or replicating in Pseudomonas sp., comprising a phi15 promoter sequence operably linked to a nucleotide comprising one or more restriction sites for the insertion of a nucleotide sequence encoding a recombinant protein, or operably linked to a nucleotide sequence encoding a recombinant protein.

This disclosure relates to anti-Pseudomonas Psl and PcrV bispecific antibodies for use in treatment of bronchiectasis.

The invention relates to the technical field of sewage treatment materials, and particularly discloses a sludge domestication nutrient solution, autotrophic denitrification sludge and a preparation method and application thereof. The sludge domestication nutrient solution provided by the invention contains various nutritional ingredients required by metabolism of different flora microorganisms, so that the problem of weakening of flora variety diversity caused by insufficient nutrient elements is avoided; the sludge domestication nutrient solution is rich in various trace elements and growth promoting factors, promotes the growth activity of various enzymes and microorganisms, also promotes the growth of microbial flora cells, enriches the types and abundance of the flora, and enhances the reproductive capacity and diversity of the flora. According to the method, thiobacillus denitrificans, pseudomonas and lysobacter in sludge are activated into dominant strains by selecting a specific sludge domestication nutrient solution, the dominant strains are applied to sewage, and the dual effects of nitrogen and phosphorus removal are achieved under the condition that no extra carbon source is added.

The invention provides a horizontal penetration type bio-electro-Fenton device and a treatment method of wastewater containing an organic collecting agent, and particularly relates to the technical field of water treatment. The horizontal penetration type bioelectro-Fenton device comprises an anode chamber, a reaction chamber and a cathode chamber which are horizontally arranged in sequence; a water inlet is formed in one side, far away from the reaction chamber, of the cathode chamber; a water outlet is formed in one side, away from the reaction chamber, of the anode chamber; a reaction chamber is defined between the cathode chamber and the anode chamber; a cathode plate is arranged in the cathode chamber, is mainly formed by assembling porous carbon felts loaded with a catalyst, and has a porous and water-permeable structure; an anode plate is arranged in the anode chamber and is composed of a carbon felt cultured with pseudomonas and bacillus microorganisms; the horizontal penetration type biological electro-Fenton device further comprises an external circuit, the external circuit is connected with the cathode plate and the anode plate, and the external circuit and the resistor element form a closed loop, so that the device spontaneously generates electricity and drives electro-Fenton reaction under the condition of no external voltage.

The present invention provides a kind of L-threonine aldolase mutant and its application in catalytic synthesis of p-methylsulfonylphenylserine, the L-threonine aldolase mutant source Pseudomonas of the present invention, can use threonine and p-methylsulfonylbenzaldehyde as substrate, with pyridoxal phosphate and reduced nicotinamide adenine dinucleotide as coenzyme, catalysis obtains the synthesis of florfenicol intermediate p-methylsulfonylphenylserine, compared to wild-type L-threonine aldolase, mutant enzyme has higher activity, can effectively improve conversion efficiency, shorten conversion time. The enzyme and technical method reaction conditions of the present invention are mild, pollution is small, and there is good industrial prospect in the production of florfenicol synthetic intermediate.

The present disclosure provides methods for predicting drug resistance in a subject with a Pseudomonas infection based on the detection of antibiotic-induced mutational signatures of Pseudomonas species.

The present disclosure relates to bacteriophages and compositions capable of infecting and killing Pseudomonas, and use of the same for treating Pseudomonas, e.g. Pseudomonas aeruginosa, bacterial infections.

The invention relates to a pseudomonas strain A18 and application thereof, the strain is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 2025521, and the strain is classified and named as Pseudomonas glycinae A18. By exogenous addition of A18, hypha growth of pure-culture lactarius deliciosus fungi can be increased, symbiosis of pine-lactarius deliciosus mycorrhiza is promoted, the number of mycorrhizal tips is increased, the plant height and biomass of mycorrhizal seedlings are improved, and meanwhile nitrogen, potassium and iron absorption of overground parts of the mycorrhizal seedlings and nitrogen absorption of root systems are increased; therefore, the method can be applied to promoting synthesis and / or growth of a pinus plant-lactarius deliciosus symbiotic system.