39 results about "Genus Pseudomonas" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to a pseudomonas TaRb6 capable of preventing diseases and promoting growth and application of the pseudomonas TaRb6. The TaRb6 strain provided by the invention belongs to a potential new species of pseudomonas, and the preservation number of the TaRb6 strain is CGMCC (China General Microbiological Culture Collection Center) No.36131. The strain TaRb6 and a fermentation inoculant prepared from the strain TaRb6 have good disease prevention and growth promotion functions on plants; the bacterial inhibition rate of sterile fermentation liquor of the strain TaRb6 on fusarium pseudograminearum reaches 46.54%-62.82%, and the bacterial colony diameter of the fusarium pseudograminearum can be reduced by 61.38% through metabolized volatile organic compounds of the strain TaRb6. After the TaRb6 bacterial suspension is used for seed soaking treatment of wheat, the incidence rate of wheat basal stem rot can be remarkably reduced, and the growth vigor of wheat can be promoted. In addition, the strain TaRb6 has broad-spectrum antibacterial ability, and the antibacterial rate of the strain TaRb6 on pathogenic bacteria of fusarium, botrytis cinerea, apical cyst and xanthomonas reaches 47.23%-67.02%.

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

A low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacteria is used for wastewater denitrification method, relates to a kind of biological bacteria for wastewater denitrification method, the bacteria is Pseudomonas umsongensis BK17, belongs to Pseudomonas, is preserved in China typical culture preservation center, and the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024.The strain is a heterotrophic nitrification-aerobic denitrification bacteria, can efficiently remove inorganic nitrogen pollution in water body, and still has good denitrification performance under low temperature stress, and has strong low temperature tolerance.The strain can efficiently treat nitrogen-containing wastewater at 10 DEG C, and the removal efficiency can reach more than 99%.In the inorganic nitrogen pollution treatment and application of various water bodies such as domestic sewage in cold regions of China, it has great potential.

Provided are bispecific antibodies that specifically recognize PcrV and / or Psl from Pseudomonas aeruginosa, pharmaceutical compositions comprising antibodies specifically recognizing non-overlapping epitopes of Pseudomonas PcrV and / or Psl. Also provided are methods of making and using these antibodies.

This invention discloses a method for coupling physicochemical treatment and biodegradation of plastics. The method includes the following steps: S1. Mixing plastics, organic solvents, and a metal catalyst, and reacting to degrade them to obtain organic products. The metal catalyst is cobalt acetate and / or manganese acetate; S2. Biodegrading the organic products from step S1 using a mixed colony, which includes *Pseudomonas*, *Bacillus*, *Flavobacterium*, and *Rhodococcus*. This invention uses cobalt acetate and / or manganese acetate as metal catalysts. The catalytic oxidation of the metal catalyst can efficiently depolymerize traditional plastics, overcoming the bottleneck of C-C bond breaking that is difficult for microorganisms to achieve. It can successfully convert plastics into organic products mainly composed of dicarboxylic acids, benzoic acid, and terephthalic acid, and the mixed colony process completely mineralizes the organic products. This method achieves complete removal of plastics without producing any toxic or harmful substances.

The invention relates to a Pseudomonas sp. strain for use in the production of a recombinant protein characterised in that said strain comprises a nucleotide sequence encoding a phi15 RNA polymerase. The invention further relates to a plasmid, capable of integrating or replicating in Pseudomonas sp., comprising a phi15 promoter sequence operably linked to a nucleotide comprising one or more restriction sites for the insertion of a nucleotide sequence encoding a recombinant protein, or operably linked to a nucleotide sequence encoding a recombinant protein.

The invention provides a horizontal penetration type bio-electro-Fenton device and a treatment method of wastewater containing an organic collecting agent, and particularly relates to the technical field of water treatment. The horizontal penetration type bioelectro-Fenton device comprises an anode chamber, a reaction chamber and a cathode chamber which are horizontally arranged in sequence; a water inlet is formed in one side, far away from the reaction chamber, of the cathode chamber; a water outlet is formed in one side, away from the reaction chamber, of the anode chamber; a reaction chamber is defined between the cathode chamber and the anode chamber; a cathode plate is arranged in the cathode chamber, is mainly formed by assembling porous carbon felts loaded with a catalyst, and has a porous and water-permeable structure; an anode plate is arranged in the anode chamber and is composed of a carbon felt cultured with pseudomonas and bacillus microorganisms; the horizontal penetration type biological electro-Fenton device further comprises an external circuit, the external circuit is connected with the cathode plate and the anode plate, and the external circuit and the resistor element form a closed loop, so that the device spontaneously generates electricity and drives electro-Fenton reaction under the condition of no external voltage.

The present disclosure relates to bacteriophages and compositions capable of infecting and killing Pseudomonas, and use of the same for treating Pseudomonas, e.g. Pseudomonas aeruginosa, bacterial infections.

The invention relates to the field of soil pollution remediation, and discloses a micro-ecological reconstruction and remediation method for micro-plastic polluted soil, which comprises the following steps: firstly, screening and enriching micro-plastic degradation functional florae which have the advantages of pseudomonas and bacillus, and the bacillus can secrete lipase and protease; the preparation method comprises the following steps: mixing biomass charcoal prepared by pyrolysis of corn straws with a functional bacterium suspension for adsorption, and carrying out sodium alginate-calcium chloride crosslinking to prepare an immobilized composite repairing agent; applying a repairing agent accounting for 1-5% of the mass of the soil, uniformly mixing the repairing agent with the soil, regulating the soil humidity to 20-30% and the carbon-nitrogen ratio to (15-25): 1, and maintaining the environment temperature of 15-35 DEG C; microplastics are removed through the synergistic effect of biomass charcoal adsorption and functional flora enzymatic degradation, beneficial microorganisms are enriched synchronously, and functional gene expression is activated; the problems that in the prior art, functional flora planting is difficult, the degradation efficiency is low, micro-ecological restoration is deficient, and the environmental adaptability is poor are solved.

The present application relates to the field of microbial technology, and specifically provides a synthetic microbial consortium B2 for preventing and treating banana wilt and application thereof. The synthetic microbial consortium B2 is composed of three antagonistic bacteria S-1-56, S-2-42 and 1-119 of Pseudomonas genus isolated from the rhizosphere soil of banana. Foc The synthetic microbial consortium B2 has a significant synergistic inhibitory effect. The synthetic microbial consortium B2 can be applied to the rhizosphere soil of banana by irrigation and the like, effectively prevents and controls the occurrence and spread of banana wilt, has the advantages of environmental friendliness, stable prevention and treatment effect, strong adaptability and the like, and is suitable for the production practice of green and sustainable agriculture.

A plasma-assisted method includes receiving pieces of waste polymer, then applying a modification process that weakens the waste polymer covalent bonds. A low temperature oxidative depolymerization process is applied to the modified polymer, which transforms the modified polymer to a short chain polymer. Optionally the short chain polymer includes C2-C2. Optionally the short chain polymer is input to a process of bacteria metabolization that produces medium-chain-length poly hydroxyalkanoates (mcl-PHAs). Optionally the bacteria is a Pseudomonas strain. Optionally the oxidative depolymerization process is performed at room temperature.

The invention belongs to the technical field of microbial prevention and control of banana diseases, and particularly discloses a microbial flora for preventing and controlling banana wilt and application thereof, a synthetic microbial flora for preventing and controlling banana wilt is composed of the following three strains of Pseudomonas sp.: Pseudomonas sp. 1-11, Pseudomonas sp. S-1-56 and Pseudomonas sp. S-2-42, and compared with a single strain, the microbial flora has the advantages that the content of the microbial flora is increased, and the content of the microbial flora is increased. The flora shows stronger colonization ability in a complex soil environment, and a more tough micro-ecological barrier can be formed through division and cooperation of strains, so that the inhibition effect on Foc TR4 is more remarkable, and the lasting time is longer. The design effectively overcomes the limitation that the effect of a single biological control strain is easy to fluctuate in practical application.

The invention discloses application of pseudomonas as a marker in evaluation of bull semen quality, and belongs to the technical field of animal breeding and microbiomics. The relative abundance of Pseudomonas in a bull rumen fluid with high semen quality is significantly higher than the relative abundance in a bull rumen fluid with low semen quality. By detecting the relative abundance, the bull semen quality can be evaluated, and the reproductive performance can be reliably judged on the premise of not depending on a semen sample. Evaluation can be carried out before semen collection for the first time or in a breeding candidate period, potential low-fecundity individuals are screened out in advance, and the breeding risk is reduced.

The invention discloses application of electrochemical active bacteria sensitive to organochlorine pollutants in detection of water quality biotoxicity, and belongs to the technical field of water quality biotoxicity detection.After a bacteria solution of the electrochemical active bacteria is exposed in toxic pollutants, the water quality biotoxicity is determined according to an electric signal inhibition rate; the toxic pollutants are organic chlorine pollutants; the electrochemical active bacterium is a pseudomonas genus, and specifically, the pseudomonas genus is Pseudomonas lopnurensis or Pseudomonas delhiensis. The invention further discloses a preparation method of the electrochemical active bacterium. The method provided by the invention is expected to improve the sensitivity of determining the biotoxicity of the organic chlorine pollutants, overcome the application bottleneck of low sensitivity, and provide technical support for biotoxicity monitoring work.

This invention discloses a compound microbial agent for processing wastewater from Trichosanthes kirilowii seeds and its preparation method, relating to the field of environmental microbiology. The compound microbial agent is composed of saponin-degrading strains, polysaccharide-degrading strains, and decolorizing strains in a volume ratio of 3:2:1. The saponin-degrading strains are Bacillus strains, the polysaccharide-degrading strains are Pseudomonas strains, and the decolorizing strains are Rhodococcus strains. The preparation method includes: isolating and screening functional strains from Trichosanthes kirilowii planting soil and wastewater from the processing workshop; expanding the culture to a bacterial concentration of CFU / mL or higher; mixing them in a volume ratio of 3:2:1; adding a protective agent; and drying to prepare a powder with a viable count greater than CFU / g. This compound microbial agent is added to the biochemical treatment unit at 0.01%-0.05% of the wastewater volume. Microbial community acclimatization can be completed within 7-14 days, effectively improving COD removal rate, saponin degradation rate, and color removal rate. It is suitable for the efficient biological treatment of Trichosanthes kirilowii seed processing wastewater.

The present application relates to a kind of high conversion rate degradation cotton stalk free gossypol enzyme combination, in view of the existing cotton stalk free gossypol strong toxicity, low degradation efficiency, lead to its resource utilization is limited, the present application obtains efficient degradation strain and compound specific enzyme preparation, constructs and forms bacteria enzyme combination system.The bacteria enzyme combination includes degradation strain fermentation liquor and composite enzyme preparation, wherein the degradation strain is the mixed strain of pseudomonas and bacillus, and the composite enzyme preparation is compounded by laccase, peroxidase and esterase according to a specific ratio.The degradation rate of the combination to free gossypol in cotton stalk can reach more than 95%, which is significantly better than the degradation effect of single strain or single enzyme preparation.

The invention discloses application of pseudomonas, and belongs to the field of microorganism application. The bacterial strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 28, 2025, the preservation number is CGMCC NO.36820, the bacterial strain belongs to Pseudomonas sp., and the name of the bacterial strain is Pseudomonas T9. The invention also discloses a preparation method of the bacterial strain. The strain has the capabilities of fixing nitrogen, producing ammonia and dissolving phosphorus, can secrete siderophores, indoleacetic acid (IAA) and protease, and has the function of promoting growth of various plants.

The application discloses a pseudomonas and an application thereof, and belongs to the field of microbial application. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 28, 2025, and the preservation number is CGMCC NO. 36820. The strain belongs to the genus Pseudomonas sp., and the strain name is Pseudomonas T9. The strain has a significant inhibiting effect on plants infected with bacterial bacterial wilt disease bacteria, and has important application potential in the prevention and control of bacterial wilt disease.

This invention provides a Pseudomonas spp. bacterial composition, a compound bacterial agent, and their applications, belonging to the field of microbial technology. This invention provides a Pseudomonas spp. bacterial composition, comprising Pseudomonas spp. bacteria (… Pseudomonas arcuscaelestis JF1 and ( Pseudomonas hutmensis JF2 and JF2 are growth-promoting bacteria screened from the rhizosphere soil of Corydalis yanhusuo. Pseudomonas bacteria JF1 and JF2 have the ability to solubilize phosphorus and produce iron carriers. As growth promoters, they can be used synergistically to promote the growth of Corydalis yanhusuo seedlings. In particular, they have a significant effect on promoting the accumulation of total alkaloids in Corydalis yanhusuo tubers. They can be used in the artificial cultivation of Corydalis yanhusuo, providing a theoretical basis for improving the yield and quality of Corydalis yanhusuo. They have important guiding significance for the rational and effective use of microorganisms to improve the quality and yield of "Qin medicine"—Corydalis yanhusuo.

The purpose of the present invention is to provide a novel enzyme for ketone body measurement that, unlike 3-hydroxybutyrate dehydrogenase, does not require NAD, which has low thermal stability, and therefore does not require a substance for reoxidizing NADH. The novel enzyme according to the present invention is a 3-hydroxybutyrate oxidase that is derived from a microorganism selected from the group consisting of microorganisms belonging to the genus Achromobacter, microorganisms belonging to the genus Pseudomonas, microorganisms belonging to the genus Trypanosoma, and microorganisms belonging to the genus Nocardia and has a physicochemical property of catalyzing a reaction for producing hydrogen peroxide by acting on D-3-hydroxybutyric acid, or is a 3-hydroxybutyrate oxidase that comprises an amino acid sequence represented by SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, or SEQ ID NO: 7, or a sequence similar thereto.

Provided herein are antibodies including antigen-binding fragments thereof that specifically recognizing Pseudomonas Psl. Also provided are methods of making and using these antibodies.

The invention belongs to the technical field of environmental engineering and water ecological restoration, and particularly relates to a river subsurface zone printing and dyeing wastewater in-situ restoration system and method. A modified adsorption material-in-situ catalytic reaction-Internet of Things intelligent operation and maintenance-ecological collaborative adaptation integrated system is adopted, an olive pomace activated carbon-montmorillonite-nano titanium dioxide composite adsorption material is adopted, and the dye interception and degradation efficiency is improved by utilizing an interlayer pore structure and photocatalytic performance; through a nano zero-valent iron composite reaction layer, high-activity green rust is generated in situ under the condition that the pH value of HCO3 <-> is 8.5-10.0, the utilization rate of Fe < 2 + > is increased, and the green rust does not need to be added from an external source; a pressure-water quality-water level three-parameter Internet of Things monitoring module is integrated, and blockage early warning and self-adaptive regulation and control are realized through a cloud platform; a reed-acorus calamus mixed planting plant community is selected and matched with a functional flora of pseudomonas and sphingomonas, the microbial degradation and ecological carbon sequestration functions are enhanced, and in-situ remediation of the subsurface flow belt printing and dyeing wastewater is achieved through multi-aspect cooperation.

The present application relates to the field of microbial technology, and specifically provides a plant growth promoting rhizobacteria NFB10 and its application. The strain is isolated from the rhizosphere soil of rubber tree in Hainan province, and is a potential new species of Pseudomonas. The similarity of 16S rDNA sequence of the strain to similar strains is higher than 99.8%, but the average nucleotide identity of whole genome between species is lower than the inter-species threshold of 95%. The strain has the abilities of phosphorus solubilization, potassium solubilization and nitrogen fixation, and can produce indole acetic acid, cellulase, amylase and ammonia and other growth promoting substances. The strain grows well in the salt concentration of 3% or less and the pH range of 5.0-9.0. Experiments show that the strain can significantly improve the germination rate, root length and sprout length of wheat seeds, and has obvious promoting effects on the plant height, ground diameter, biomass and chlorophyll content of the leaves of the seedlings of Hevea brasiliensis. The strain can be used for preparing plant growth promoting bacteria agents, and is suitable for green cultivation and yield improvement of rubber trees and other crops.

The present invention relates to a novel bacterium Pseudomonas sp. 14og as deposited into the NRRL Patent Culture Collection on 3 Dec. 2020 and designated NRRL accession no. B-67999. The present invention also relates to insecticidally active substances obtained or derived from Pseudomonas sp. 14og. The insecticidal substances find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, Hemipteran, Tetranychidae, fungal, nematode and mite pest populations and for producing compositions with insecticidal activity. The present invention relates to a novel bacterium and use for biocontrol of insect pests. More particularly, the invention relates to a newly discovered species of Pseudomonas bacterium that exhibits insecticidal activity. The invention also relates to proteins and / or peptides and / or metabolites obtained from the strain that possess insecticidal activity and to insecticidal compositions comprising cultures of the novel strain and / or supernatants, filtrates, and extracts obtained from the strain, and use thereof to control insect pests. The present invention relates to a novel bacterium and uses thereof. In particular, the present invention relates to applications concerned with the biopesticidal activity of the novel bacterium and of its mutant or variant strains. The present invention also concerns proteinaceous substances produced by this novel bacterium and of its mutant or variant strains obtained or derived from the aforesaid bacteria.

The application relates to the technical field of microbial remediation methods, and specifically discloses an ex-situ microbial remediation method for river sewage, aiming to solve the multiple contradictions of redundancy of existing ex-situ microbial remediation technology, single function of bacterial flora, weak environmental adaptability and difficulty in giving consideration to high efficiency and engineering simplicity. The method comprises the following steps: guiding the river sewage out and pretreating the river sewage to remove large-particle impurities; introducing a biological reactor to inoculate a composite functional bacterial flora composed of the genus Raoultella, the genus Pseudomonas and the genus Bacillus in proportion; implementing gradient dissolved oxygen regulation to realize simultaneous nitrification and denitrification; adding iron-manganese modified biochar as a microbial carrier and an electron mediator; and recycling the biochar after solid-liquid separation and reaction. Through the above technical scheme, the application can realize multi-target and simultaneous high-efficiency removal of ammonia nitrogen, refractory organic matter and trace heavy metals, simplify a process flow, improve system stability and low-temperature adaptability, and support modular deployment and intelligent operation and maintenance.

The invention discloses pseudomonas for antagonizing ralstonia solanacearum and application thereof, and belongs to the technical field of microorganisms. The Pseudomonas sp. SHP-38 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is January 19, 2026, and the preservation number is CGMCC No.37456. The Pseudomonas sp. SHP-38 disclosed by the invention has the advantages that the preservation number is CGMCC No.37456; through physiological and biochemical identification and molecular taxonomy identification, it is confirmed that the pseudomonas SHP-38 belongs to a new species of pseudomonas, and species resources and genetic diversity of pseudomonas flora are expanded. The pseudomonas sp. SHP-38 has double effects of inhibiting the growth of ralstonia solanacearum and promoting the growth of plants, has the potential of being developed into a microbial agent for the eucalyptus bacterial wilt, is beneficial to reducing the use of chemical pesticides, provides a new biological control technology for solving the eucalyptus bacterial wilt, and has a wide application prospect.

The application discloses a kind of septal inflammation pseudomonas LH-Cr-2, belong to microbial technical field.The biological preservation number of septal inflammation pseudomonas LH-Cr-2 is CGMCC NO.27552.The application also discloses the fermentation culture method of septal inflammation pseudomonas LH-Cr-2 for use as a way.The septal inflammation pseudomonas LH-Cr-2 of the application can efficiently reduce different concentrations of soluble hexavalent chromium, provides experimental technology and theoretical basis for the application of microbial reduction technology in chromium-contaminated soil remediation.Six valence chromium is efficiently reduced, and in-situ remediation of chromium-contaminated soil or groundwater can be applied, and can also be used in other related fields that can solve problems by reducing hexavalent chromium.

The application belongs to the field of bioengineering, and discloses a lactose oxidase mutant with improved enzyme activity and application in the production of lactobionic acid. The mutant is based on wild-type lactose oxidase from Pseudomonas, and is obtained by site-directed mutation of phenylalanine (F) at position 312 of the amino acid sequence to tyrosine (Y). After induction expression and purification, the mutant enzyme activity is about 1.8 times higher than that of the wild type. The lactose oxidase mutant provided by the application can realize efficient biological conversion of lactose to lactobionic acid, and provides a feasible technical path for green, sustainable and large-scale production of lactobionic acid, and has good industrial application prospects.

The objective is to provide a wastewater purification solution for seafood processing plants that purifies wastewater, prevents sludge buildup, and prevents foul odors from being generated from the wastewater. [Solution] The solution is a mixture containing a culture medium obtained by culturing an aqueous solution of molasses with 10 species of bacteria from the genera Aspergillus, Bacillus, Lactopranthibacillus, Enterococcus, Nocardia, Pseudomonas, Ralstonia, Rhizopus, Rhodococcus, and Rhodosprillum, as well as iron fulvic acid, molasses, seaweed powder, and water.

The application belongs to the polymer field and discloses application of a nanocellulose / chitosan active packaging film in preservation of Russula, wherein the nanocellulose / chitosan active packaging film is prepared by mixing Ag@MOF carriers loaded with natural antibacterial agent natal oil and nanocellulose / chitosan compound, and the obtained product has good tensile strength, elongation at break and water vapor permeability, and shows good film ductility and gas barrier performance; the packaging film is applied to Russula packaging, can effectively inhibit the growth of specific spoilage bacteria such as Pseudomonas, Serratia and Penicillium during the storage of Russula, so as to achieve the purpose of preserving Russula, and can slowly release the natal oil, thereby prolonging the preservation period of Russula.