65 results about "Genus Pseudomonas" patented technology

The invention discloses pseudomonas capable of dissolving phosphorus and producing siderophores and application of the pseudomonas, and relates to the technical field of microorganisms. The bacterial strain P-14-21 is identified as a new species of Pseudomonas sp. And is named as Pseudomonas sp. P-14-21, the bacterial strain is preserved in Guangdong Microbial Culture Collection Center on August 7, 2024, and the preservation number is GDMCC No: 64969. The invention also discloses a preparation method of the Pseudomonas sp. P-14-21, and the bacterial strain is named as Pseudomonas sp. P-14-21. The bacterial strain is named as Pseudomonas sp. P-14-21 and is preserved in Guangdong Microbial Culture Collection Center on August 7, 2024. Besides, the pseudomonas P-14-21 disclosed by the invention has the capacity of dissolving organic phosphorus and high-yield siderophore, the phosphorus dissolving index can reach 2.4, and the activity unit (su) of the siderophore can reach 94.41%, so that the strain has excellent development and application values in agricultural production.

The invention discloses preparation of a microbial compound element-based fertilizer and application of the microbial compound element-based fertilizer in saline-alkali soil treatment, and relates to the technical field of saline-alkali soil improvement. The preparation method comprises the following steps: inoculating lactic acid bacteria, rhodopseudomonas, phosphate-solubilizing bacillus, nitrogen-fixing bacillus, halophilic bacillus and halomonas into a composite culture medium in proportion, and carrying out stepped sequential fermentation culture under the conditions that the pH value is 7.5 and the temperature is 28 DEG C until the bacterial count is greater than or equal to 1010 CFU / mL. According to the preparation of the fertilizer based on the microbial compound elements and the application of saline-alkali soil treatment, a synergistic system of the compound functional flora, the chelated mineral substances and the intelligent organic carrier is constructed, so that multi-dimensional improvement of saline-alkali soil is realized, the limitation of a traditional single improvement mode is broken through, the salinity and pH value of the soil are effectively reduced, and the soil quality is improved. The soil fertility and biological activity are remarkably improved, the salt stress resistance of crops is enhanced, the effective period is prolonged, and the preparation cost is low.

The invention belongs to the technical field of microorganisms, and particularly relates to a pseudomonas TaRb6 capable of preventing diseases and promoting growth and application of the pseudomonas TaRb6. The TaRb6 strain provided by the invention belongs to a potential new species of pseudomonas, and the preservation number of the TaRb6 strain is CGMCC (China General Microbiological Culture Collection Center) No.36131. The strain TaRb6 and a fermentation inoculant prepared from the strain TaRb6 have good disease prevention and growth promotion functions on plants; the bacterial inhibition rate of sterile fermentation liquor of the strain TaRb6 on fusarium pseudograminearum reaches 46.54%-62.82%, and the bacterial colony diameter of the fusarium pseudograminearum can be reduced by 61.38% through metabolized volatile organic compounds of the strain TaRb6. After the TaRb6 bacterial suspension is used for seed soaking treatment of wheat, the incidence rate of wheat basal stem rot can be remarkably reduced, and the growth vigor of wheat can be promoted. In addition, the strain TaRb6 has broad-spectrum antibacterial ability, and the antibacterial rate of the strain TaRb6 on pathogenic bacteria of fusarium, botrytis cinerea, apical cyst and xanthomonas reaches 47.23%-67.02%.

Modified bacterial strain of the Pseudomonas sp. genus, the strain of Pseudomonas stutzeri deposited under Deposit Accession No. RGM3505 and a modified bacterial strain of the Bacillus sp. genus, a strain of Bacillus velezensis deposited under Accession No. RGM3504. The strains may be combined at a Pseudomonas stutzeri to Bacillus velezensis ratio from 1 :10 to 10:1 CFU / mL. The strains were modified to have greater chemotactic attraction towards plant roots and to improve the beneficial features of crops, for example, of legumes.

To provide a detection method capable of rapidly detecting drug-resistant bacteria, bacteria of the genus Staphylococcus, and bacteria of the genus Pseudomonas; a method for providing an index for selecting an antimicrobial agent for bacteria; and a primer set usable in these methods.SOLUTION: The detection method for drug-resistant bacteria, bacteria of the genus Staphylococcus, and bacteria of the genus Pseudomonas according to the present disclosure comprises a step of performing nucleic acid amplification of target genes (1) to (3) and (4) below for a target sample, and detecting drug-resistant bacteria, bacteria of the genus Staphylococcus, and bacteria of the genus Pseudomonas in the sample: (1) the mecA gene; (2) the spa gene of Staphylococcus aureus; (3) the tuf gene and / or the rpoB gene of bacteria of the genus Staphylococcus; and (4) the 16S rRNA gene of bacteria of the genus Pseudomonas.SELECTED DRAWING: Figure 1

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

The application provides a method for separating and purifying 3-hydroxydecanoic acid from Pseudomonas bacteria. First, the fermentation product of Pseudomonas bacteria is decomposed by alkali to obtain monomers of 3-hydroxydecanoic acid. Then, activated carbon is used for decolorization, and microfiltration membrane and ultrafiltration membrane are used for treatment to remove macromolecular impurities. Finally, a sodium 3-hydroxydecanoate solution is loaded onto a polystyrene-divinylbenzene macroporous resin column modified by polyethyleneimine, and eluted with an ethanol-water (volume ratio 1:1) solution with a pH of 11. The eluate is collected, ethanol is evaporated, a purified sodium 3-hydroxydecanoate solution is obtained, hydrochloric acid is added to adjust the pH to 3-4, and the precipitate is collected by centrifugation and dried to obtain a 3-hydroxydecanoic acid product. The content of 3-hydroxydecanoic acid is detected by liquid chromatography, and the total content of 3-hydroxydecanoic acid in the purified product is higher than 90%. The method uses less organic solvent, the steps are simple, the purity of the obtained product is high, and the application requirements of cosmetics and pharmaceutical intermediates are met.

A low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacteria is used for wastewater denitrification method, relates to a kind of biological bacteria for wastewater denitrification method, the bacteria is Pseudomonas umsongensis BK17, belongs to Pseudomonas, is preserved in China typical culture preservation center, and the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024.The strain is a heterotrophic nitrification-aerobic denitrification bacteria, can efficiently remove inorganic nitrogen pollution in water body, and still has good denitrification performance under low temperature stress, and has strong low temperature tolerance.The strain can efficiently treat nitrogen-containing wastewater at 10 DEG C, and the removal efficiency can reach more than 99%.In the inorganic nitrogen pollution treatment and application of various water bodies such as domestic sewage in cold regions of China, it has great potential.

Provided are bispecific antibodies that specifically recognize PcrV and / or Psl from Pseudomonas aeruginosa, pharmaceutical compositions comprising antibodies specifically recognizing non-overlapping epitopes of Pseudomonas PcrV and / or Psl. Also provided are methods of making and using these antibodies.

This invention discloses a method for coupling physicochemical treatment and biodegradation of plastics. The method includes the following steps: S1. Mixing plastics, organic solvents, and a metal catalyst, and reacting to degrade them to obtain organic products. The metal catalyst is cobalt acetate and / or manganese acetate; S2. Biodegrading the organic products from step S1 using a mixed colony, which includes *Pseudomonas*, *Bacillus*, *Flavobacterium*, and *Rhodococcus*. This invention uses cobalt acetate and / or manganese acetate as metal catalysts. The catalytic oxidation of the metal catalyst can efficiently depolymerize traditional plastics, overcoming the bottleneck of C-C bond breaking that is difficult for microorganisms to achieve. It can successfully convert plastics into organic products mainly composed of dicarboxylic acids, benzoic acid, and terephthalic acid, and the mixed colony process completely mineralizes the organic products. This method achieves complete removal of plastics without producing any toxic or harmful substances.

The invention relates to a Pseudomonas sp. strain for use in the production of a recombinant protein characterised in that said strain comprises a nucleotide sequence encoding a phi15 RNA polymerase. The invention further relates to a plasmid, capable of integrating or replicating in Pseudomonas sp., comprising a phi15 promoter sequence operably linked to a nucleotide comprising one or more restriction sites for the insertion of a nucleotide sequence encoding a recombinant protein, or operably linked to a nucleotide sequence encoding a recombinant protein.

This disclosure relates to anti-Pseudomonas Psl and PcrV bispecific antibodies for use in treatment of bronchiectasis.

The invention provides a horizontal penetration type bio-electro-Fenton device and a treatment method of wastewater containing an organic collecting agent, and particularly relates to the technical field of water treatment. The horizontal penetration type bioelectro-Fenton device comprises an anode chamber, a reaction chamber and a cathode chamber which are horizontally arranged in sequence; a water inlet is formed in one side, far away from the reaction chamber, of the cathode chamber; a water outlet is formed in one side, away from the reaction chamber, of the anode chamber; a reaction chamber is defined between the cathode chamber and the anode chamber; a cathode plate is arranged in the cathode chamber, is mainly formed by assembling porous carbon felts loaded with a catalyst, and has a porous and water-permeable structure; an anode plate is arranged in the anode chamber and is composed of a carbon felt cultured with pseudomonas and bacillus microorganisms; the horizontal penetration type biological electro-Fenton device further comprises an external circuit, the external circuit is connected with the cathode plate and the anode plate, and the external circuit and the resistor element form a closed loop, so that the device spontaneously generates electricity and drives electro-Fenton reaction under the condition of no external voltage.

The present invention provides a kind of L-threonine aldolase mutant and its application in catalytic synthesis of p-methylsulfonylphenylserine, the L-threonine aldolase mutant source Pseudomonas of the present invention, can use threonine and p-methylsulfonylbenzaldehyde as substrate, with pyridoxal phosphate and reduced nicotinamide adenine dinucleotide as coenzyme, catalysis obtains the synthesis of florfenicol intermediate p-methylsulfonylphenylserine, compared to wild-type L-threonine aldolase, mutant enzyme has higher activity, can effectively improve conversion efficiency, shorten conversion time. The enzyme and technical method reaction conditions of the present invention are mild, pollution is small, and there is good industrial prospect in the production of florfenicol synthetic intermediate.

The present disclosure provides methods for predicting drug resistance in a subject with a Pseudomonas infection based on the detection of antibiotic-induced mutational signatures of Pseudomonas species.

The present disclosure relates to bacteriophages and compositions capable of infecting and killing Pseudomonas, and use of the same for treating Pseudomonas, e.g. Pseudomonas aeruginosa, bacterial infections.

The invention relates to the field of soil pollution remediation, and discloses a micro-ecological reconstruction and remediation method for micro-plastic polluted soil, which comprises the following steps: firstly, screening and enriching micro-plastic degradation functional florae which have the advantages of pseudomonas and bacillus, and the bacillus can secrete lipase and protease; the preparation method comprises the following steps: mixing biomass charcoal prepared by pyrolysis of corn straws with a functional bacterium suspension for adsorption, and carrying out sodium alginate-calcium chloride crosslinking to prepare an immobilized composite repairing agent; applying a repairing agent accounting for 1-5% of the mass of the soil, uniformly mixing the repairing agent with the soil, regulating the soil humidity to 20-30% and the carbon-nitrogen ratio to (15-25): 1, and maintaining the environment temperature of 15-35 DEG C; microplastics are removed through the synergistic effect of biomass charcoal adsorption and functional flora enzymatic degradation, beneficial microorganisms are enriched synchronously, and functional gene expression is activated; the problems that in the prior art, functional flora planting is difficult, the degradation efficiency is low, micro-ecological restoration is deficient, and the environmental adaptability is poor are solved.

The present invention relates to a composition for scalp improvement, the composition being prepared by dually culturing Pseudomonas sp. microorganisms and Micrococcus sp. microorganisms in a medium composition containing saccharides and amino acids. The composition for scalp improvement has excellent scalp improvement effects such as hair growth promotion, hair loss prevention, hair root strengthening, and the like, and thus can be effectively used in cosmetics, medicines, and the like.

The present application relates to the field of microbial technology, and specifically provides a synthetic microbial consortium B2 for preventing and treating banana wilt and application thereof. The synthetic microbial consortium B2 is composed of three antagonistic bacteria S-1-56, S-2-42 and 1-119 of Pseudomonas genus isolated from the rhizosphere soil of banana. Foc The synthetic microbial consortium B2 has a significant synergistic inhibitory effect. The synthetic microbial consortium B2 can be applied to the rhizosphere soil of banana by irrigation and the like, effectively prevents and controls the occurrence and spread of banana wilt, has the advantages of environmental friendliness, stable prevention and treatment effect, strong adaptability and the like, and is suitable for the production practice of green and sustainable agriculture.

The present invention relates to an identified and isolated bacteria or biologically pure bacterial culture of the genus Pseudomonas. The invention further relates to compositions such as fertilizers and seed coatings comprising said bacteria and uses thereof, such as plant growth promoting agents.

The invention provides a pseudomonas bacterium composition, a complex microbial inoculant and application of the pseudomonas bacterium composition and the complex microbial inoculant, and belongs to the technical field of microorganisms. The invention provides a Pseudomonas bacteria combination, which comprises Pseudomonas bacteria JF1 and Pseudomonas hutmensis JF2, the Pseudomonas bacteria JF1 and the Pseudomonas hutmensis JF2 are growth-promoting bacteria screened from rhizosphere soil of rhizoma corydalis, and the Pseudomonas bacteria JF1 and the Pseudomonas bacteria JF2 have the capabilities of dissolving phosphorus and producing siderophores, can be used as growth promoting agents in a synergistic manner to promote the growth of rhizoma corydalis seedlings, and can be used for promoting the growth of rhizoma corydalis seedlings. The method has a remarkable effect of promoting accumulation of the total alkaloid content in rhizoma corydalis tubers, can be used for artificial cultivation of rhizoma corydalis, provides a theoretical basis for improving the yield and quality of rhizoma corydalis, and has important guiding significance for reasonably and effectively utilizing microorganisms to improve the quality and yield of Qinshi medicine-rhizoma corydalis.

A plasma-assisted method includes receiving pieces of waste polymer, then applying a modification process that weakens the waste polymer covalent bonds. A low temperature oxidative depolymerization process is applied to the modified polymer, which transforms the modified polymer to a short chain polymer. Optionally the short chain polymer includes C2-C2. Optionally the short chain polymer is input to a process of bacteria metabolization that produces medium-chain-length poly hydroxyalkanoates (mcl-PHAs). Optionally the bacteria is a Pseudomonas strain. Optionally the oxidative depolymerization process is performed at room temperature.

The invention belongs to the technical field of microbial prevention and control of banana diseases, and particularly discloses a microbial flora for preventing and controlling banana wilt and application thereof, a synthetic microbial flora for preventing and controlling banana wilt is composed of the following three strains of Pseudomonas sp.: Pseudomonas sp. 1-11, Pseudomonas sp. S-1-56 and Pseudomonas sp. S-2-42, and compared with a single strain, the microbial flora has the advantages that the content of the microbial flora is increased, and the content of the microbial flora is increased. The flora shows stronger colonization ability in a complex soil environment, and a more tough micro-ecological barrier can be formed through division and cooperation of strains, so that the inhibition effect on Foc TR4 is more remarkable, and the lasting time is longer. The design effectively overcomes the limitation that the effect of a single biological control strain is easy to fluctuate in practical application.

The invention discloses application of pseudomonas as a marker in evaluation of bull semen quality, and belongs to the technical field of animal breeding and microbiomics. The relative abundance of Pseudomonas in a bull rumen fluid with high semen quality is significantly higher than the relative abundance in a bull rumen fluid with low semen quality. By detecting the relative abundance, the bull semen quality can be evaluated, and the reproductive performance can be reliably judged on the premise of not depending on a semen sample. Evaluation can be carried out before semen collection for the first time or in a breeding candidate period, potential low-fecundity individuals are screened out in advance, and the breeding risk is reduced.

This invention discloses a *Pseudomonas* strain with phosphate-solubilizing and siderophore-producing capabilities and its applications, relating to the field of microbial technology. The strain P-14-21 has been identified as belonging to the genus *Pseudomonas* (…). Pseudomonas A new species of sp., and named Pseudomonas sp. P-14-21, this strain was deposited at the Guangdong Provincial Microbial Culture Collection Center on August 7, 2024, with accession number GDMCC No:64969. Furthermore, the Pseudomonas P-14-21 disclosed in this invention possesses the ability to dissolve organic phosphorus and produce high levels of siderophores, with a phosphorus solubility index reaching 2.4 and a siderophore activity unit (…). su The efficiency can reach 94.41%, therefore this strain has excellent development and application value in agricultural production.

The invention discloses application of electrochemical active bacteria sensitive to organochlorine pollutants in detection of water quality biotoxicity, and belongs to the technical field of water quality biotoxicity detection.After a bacteria solution of the electrochemical active bacteria is exposed in toxic pollutants, the water quality biotoxicity is determined according to an electric signal inhibition rate; the toxic pollutants are organic chlorine pollutants; the electrochemical active bacterium is a pseudomonas genus, and specifically, the pseudomonas genus is Pseudomonas lopnurensis or Pseudomonas delhiensis. The invention further discloses a preparation method of the electrochemical active bacterium. The method provided by the invention is expected to improve the sensitivity of determining the biotoxicity of the organic chlorine pollutants, overcome the application bottleneck of low sensitivity, and provide technical support for biotoxicity monitoring work.

This invention discloses a compound microbial agent for processing wastewater from Trichosanthes kirilowii seeds and its preparation method, relating to the field of environmental microbiology. The compound microbial agent is composed of saponin-degrading strains, polysaccharide-degrading strains, and decolorizing strains in a volume ratio of 3:2:1. The saponin-degrading strains are Bacillus strains, the polysaccharide-degrading strains are Pseudomonas strains, and the decolorizing strains are Rhodococcus strains. The preparation method includes: isolating and screening functional strains from Trichosanthes kirilowii planting soil and wastewater from the processing workshop; expanding the culture to a bacterial concentration of CFU / mL or higher; mixing them in a volume ratio of 3:2:1; adding a protective agent; and drying to prepare a powder with a viable count greater than CFU / g. This compound microbial agent is added to the biochemical treatment unit at 0.01%-0.05% of the wastewater volume. Microbial community acclimatization can be completed within 7-14 days, effectively improving COD removal rate, saponin degradation rate, and color removal rate. It is suitable for the efficient biological treatment of Trichosanthes kirilowii seed processing wastewater.

The present application relates to a kind of high conversion rate degradation cotton stalk free gossypol enzyme combination, in view of the existing cotton stalk free gossypol strong toxicity, low degradation efficiency, lead to its resource utilization is limited, the present application obtains efficient degradation strain and compound specific enzyme preparation, constructs and forms bacteria enzyme combination system.The bacteria enzyme combination includes degradation strain fermentation liquor and composite enzyme preparation, wherein the degradation strain is the mixed strain of pseudomonas and bacillus, and the composite enzyme preparation is compounded by laccase, peroxidase and esterase according to a specific ratio.The degradation rate of the combination to free gossypol in cotton stalk can reach more than 95%, which is significantly better than the degradation effect of single strain or single enzyme preparation.

The invention discloses application of pseudomonas, and belongs to the field of microorganism application. The bacterial strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 28, 2025, the preservation number is CGMCC NO.36820, the bacterial strain belongs to Pseudomonas sp., and the name of the bacterial strain is Pseudomonas T9. The invention also discloses a preparation method of the bacterial strain. The strain has the capabilities of fixing nitrogen, producing ammonia and dissolving phosphorus, can secrete siderophores, indoleacetic acid (IAA) and protease, and has the function of promoting growth of various plants.

The application discloses a pseudomonas and an application thereof, and belongs to the field of microbial application. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 28, 2025, and the preservation number is CGMCC NO. 36820. The strain belongs to the genus Pseudomonas sp., and the strain name is Pseudomonas T9. The strain has a significant inhibiting effect on plants infected with bacterial bacterial wilt disease bacteria, and has important application potential in the prevention and control of bacterial wilt disease.