153 results about "Genus Pseudomonas" patented technology

Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.

Provided herein are systems for treating a subject with a pulmonary infection, for example, a nontuberculous mycobacterial pulmonary infection, a Burkholderia pulmonary infection, a pulmonary infection associated with bronchiectasis, or a Pseudomonas pulmonary infection. The system includes a pharmaceutical formulation comprising a liposomal aminoglycoside dispersion, and the lipid component of the liposomes consist essentially of electrically neutral lipids. The system also includes a nebulizer which generates an aerosol of the pharmaceutical formulation at a rate greater than about 0.53 gram per minute. The aerosol is delivered to the subject via inhalation for the treatment of the pulmonary infection.

The invention discloses an air purifying material of microorganism absorption degradation, a preparation method and applications thereof. The air purifying material of microorganism absorption degradation is obtained by embedding biological grains with 2 to 4mm grain diameter of pseudomonades or acinetobacter which are cultured and acclimatized in a crosswise way in calcium alginate gel. The air purifying material of microorganism absorption degradation can be used in the long time purification of formaldehyde and benzene series pollution in rooms with obvious effect. The product has the advantages of high activity of immobilized cells, good stability, effectively reducing the content of chemical pollution of formaldehyde and benzene series pollution in rooms, being beneficial for realizing the recycling, regenerating and reusing of biocatalysts, and seriously avoiding the pollution to room environment by microorganisms. The raw material has low cost, the preparation process is simple, easy and convenient in operation, and product can be applied in different reactors.

The invention provides mutated, cytotoxic forms of Pseudomonas exotoxin A (PE) comprising a furin cleavage sequence conjugated or fused directly to residues 395-613 of PE or variants of that sequence. These minimal forms of PE are smaller than previous cytotoxic forms of PE, reduce non-specific toxicity, and reduce immunogenicity due to domain II or domain Ib of PE. The invention further provides nucleic acids encoding said PEs, chimeric molecules containing them, and methods of use thereof.

The invention provides chimeric proteins comprising a non-toxic Pseudomonas exotoxin A sequence and a Type IV pilin loop sequence, wherein the Type IV loop sequence is inserted within the non-toxic Pseudomonas exotoxin A. The invention also provides polynucleotides encoding the chimeric proteins, and compositions comprising the polynucleotides or the chimeric proteins. The invention also provides methods for using the chimeric proteins, polynucleotides and compositions of the invention.

The invention provides pseudomonas with the collection number of CGMCC (China General Microbiological Culture Collection Center) No.4793, a microbial agent containing the pseudomonas, and applications of the pseudomonas or the microbial agent in petroleum hydrocarbon degradation, petroleum pollution bioremediation, petroleum polluted soil bioremediation and petroleum polluted water bioremediation. The pseudomonas with the collection number of CGMCC No.4793 and the microbial agent containing the pseudomonas have strong degrading capability for the petroleum hydrocarbon, can be widely applied to petroleum hydrocarbon degradation and petroleum pollution renovation, petroleum polluted soil renovation, petroleum polluted water renovation and the like.

A method of preventing or treating infection by a range of fungal pathogens in an animal or plant or stored plant product comprising administering an effective amount of a sugar acid, in particular mannonic acid, gluconic acid or galacturonic. The sugar acid can be delivered by a biocontrol that can manufacture the sugar acid. An exemplified biocontrol is Pseudomonas strain AN5, which is characterized by the capacity to convert aldose to sugar acid. Also disclosed are nucleotide sequences from enzymes in the Pseudomonas PQQ enzyme pathway which may be used to transform microorganisms and plants so that they produce sugar acids at levels sufficient to control the growth or viability of pathogens.

The invention relates to a plasmid characterized in that the plasmid comprises a DNA fragment containing a gene coding for an enzyme taking PQQ as the prosthetic group as cloned in a broad-host-range vector defected of conjugative transfer function beforehand and that the plasmid is capable of being expressed in bacteria of the genus Pseudomonas.

The invention discloses application of elsholtzia volatile oil in anti-bacteria and antibiotic resistant bacteria. The elsholtzia volatile oil is extracted by steam distillation. The elsholtzia volatile oil has certain functions of killing and/or inhibiting growth on bacteria of staphylococcus, escherichia coli, candida, pseudomonas, enterobacter, streptococcus and the like and fungi. The elsholtzia volatile oil has certain functions of killing and/or inhibiting growth on bacteria of drug-resistant staphylococcus, drug-resistant escherichia coli, drug-resistant enterobacter, drug-resistant klebsiella, drug-resistant pseudomonas, drug-resistant acinetobacter, drug-resistant streptococcus, and drug-resistant enterococcus and the like. The elsholtzia volatile oil can be used in industries offood, medicine, health care products, cosmetics and the like and can be used for preparing various forms of products with antibacterial and antibiotic resistant bacteria functions, and belongs to thenew application field of the elsholtzia volatile oil.

The invention discloses a Pseudomonas phosphate dissolving bacterium Y2, a bioorganic fertilizer prepared through using the bacterium, and an application of the fertilizer. The above bacterium strain is separated from potato rhizosphere, and is preserved in China General Microbiological Culture Collection Center on Dec. 25, 2014 with the preservation number of CGMCC NO.10242. A result of rocking bottle experiments show that the bacterium strain can effectively reduce the pH value of a solution and dissolve insoluble phosphate in the solution; and a result of bacterium filled pot test shows that the bacterium strain can effectively promote growth of potato, corn and tobacco plants, and can massively colonize at the rhizosphere of the plants to realize a growth promotion effect. The granular bioorganic fertilizer developed through using the bacterium strain, decomposed chicken manure and rapeseed meal can be applied to substantially promote the growth of the corn plants.

The invention relates to cadmium-resistant bacteria and a method for inhibiting heavy metal cadmium absorption of paddy rice, and belongs to the technical field of agricultural and environmental pollution treatment. The cadmium-resistant bacteria are (Pseudomonas sp.) TCd-1, is preserved in the China center for type culture collection of Wuhan university on December 11, 2013 and has the accession number of CCTCC M2013649. An identification result shows that the bacterial strain TCd-1 is Pseudomonas sp., has resistance to a plurality of heavy metals, and has cadmium, lead and nickel resistance concentrations of 900mg.L<-1>, 800mg.L<-1> and 600mg.L<-1>. A ratio of the number ratio active live bacteria in the bacterial liquid is 1.85-1.87*10<9> per milliliter. Through use of 2mL of the bacterial liquid in each kg of heavy metal cadmium-polluted wet soil, cadmium-caused paddy rice growth inhibition is overcome, cadmium content of all parts of the paddy rice is effectively reduced and especially, cadmium content of brown rice is reduced.

The present invention relates to microbial preparation for promoting germination of ephedra seed. Two kinds of known bacteria, i.e., yellow pseudomonads and Bacillus subtilis, are separated and screened form rhizospheric soil of wild ephedra and through culture in medium and other steps, the microbial preparation for soaking ephedra seed is prepared. When it is used in soaking ephedra seed, the seedling stage can be 4-5 days brought forward and the seedling rate may be 13-19% raised. When it is used for processing ephedra seedling before being transplanted, it can promote the taking root and tillering and transplanted ephedra seedling.

The invention discloses a method for culturing high-density Rhodopseudomonas photosynthetic bacteria by using cyanobacterial bloom, which comprises the following steps: acquiring cyanobacterial bloom from a cyanobacterial bloom generating water body, and concentrating properly until the total nitrogen concentration in the concentrated cyanobacterial bloom system is 230-2010 mg/L and the total phosphorus concentration is 35-350 mg/L; and putting in an open transparent container in a light environment, and continuously aerating while keeping the concentrated cyanobacterial bloom system at 20-42 DEG C to form an coexistence of an aerobic region and an anaerobic region until the high-density photosynthetic bacteria are generated. The method can be used for converting the cyanobacterial bloom into the Rhodopseudomonas photosynthetic bacteria, is capable of promoting the innocent treatment and utilization of water body nutrient substances and accelerating the conversion of the water body nutrient substances, and can be directly used for treating the cyanobacterial bloom; and the formed photosynthetic bacteria can be directly used for breeding industry or sewage purification.

The invention provides a Pseudomonadaceae petroleum degrading bacterium L-1, a microbial mixed agent of the Pseudomonadaceae petroleum degrading bacterium L-1 and an in-situ remediation method of petroleum-contaminated soil. The preservation number of the Pseudomonadaceae petroleum degrading bacterium is CGMCC NO.16557. The microbial mixed agent contains the Pseudomonadaceae petroleum degrading bacteria in the preservation number being CGMCC NO.16557 and indigenous bacteria. By the aid of the method, the content of petroleum hydrocarbon and polycyclic aromatic hydrocarbon in petroleum-containing sludge can be reduced effectively, harmless treatment of the petroleum-containing sludge can be realized, harms caused by the contaminated soil to human bodies can be eliminated, and ecological functions of the soil can be restored. Thus, degradation of the petroleum hydrocarbon is used as a starting point, microbes having the obvious degrading effect on soil petroleum are separated and cultivated, and the specific implementation measure of an on-site petroleum-contaminated soil microbial in-situ remediation technology is further provided. The petroleum-contaminated soil with the concentration being 3% can be degraded by 77% after 30 days.

The invention provides low temperature resistant cellulose degradation bacteria, which are pseudomonas (Pseudomonas sp.) bacterial strains B6-15, wherein the preservation number is CGMCC NO.5851. By adopting the bacteria, the cellulose can be efficiently degraded in a low-temperature condition (15-20 DEG C); and a new microbial resource and a technical support are provided for facilitating rise of compost temperature by degrading the cellulose in the composting process in a low-temperature environment.

The invention aims to provide a microbiological degradation method for nitrobenzene industrial wastewater. According to experimental selection, composite bacteria liquid of Pseudomonas, Stenotrophomonas maltophilia, Acinetobacter baumannii, Aquaspirillum, Acidovorax avenae, Alcaligenes faecalis and Bacillus is provided. Domestication culture and three-stage amplification culture are carried out on the composite bacteria liquid so as to obtain a high efficiency composite microbial inoculum suitable for industrial production. When used to treat nitrobenzene wastewater, the microbial inoculum provided in the invention has the advantages of strong biological activity, long survival time, high capability in resisting impact load of influent quality and thorough treatment.

The invention relates to a plant-microorganism combined remediation method for a moderate and mild lindane-cadmium combined polluted farmland. Cadmium hyperaccumulators and crops are planted on polluted soil, a compound fertilizer and a biochar base fertilizer are applied, the carbon-nitrogen ratio of the soil is regulated while the pH value of the soil is reduced by using the biochar base fertilizer, the absorption of the hyperaccumulators to pollutants isenhanced while the biomass of the hyperaccumulators is improved, the growth of the hyperaccumulators is not influenced by concentration aggregation of the pollutants, pseudomonas and arbuscular mycorrhizal fungi are prepared into a mixed microbial inoculum, and the resistance of the hyperaccumulators is improved; and economic crops with root systems deeper than those of the hyperaccumulators and the hyperaccumulators are selected for intercropping/relay intercropping, the soil fertility is improved, the growth environment of the hyperaccumulators is adjusted, the remediation efficiency of the hyperaccumulators on lindane and cadmium is further enhanced, and the purpose of remediation while production is achieved.

The invention relates to preparation and an application of an aerobic denitrifying bacterial agent for enhancing a lakeside wetland nitrogen removal function, and belongs to the fields of bioengineering technology and environmental protection. The invention provides the aerobic denitrifying bacterial agent for enhancing the lakeside wetland nitrogen removal function, wherein the bacterial agent includes 3 strains (WF065, WF084, WF092) of pseudomonas spp., and is obtained by separating and screening from Dian lake lakeside wetland plant root system soil. Culture materials of the 3 pseudomonas spp. strains are mixed according to the weight ratio of 1:1:1. The prepared aerobic denitrifying bacterial agent can obviously enhance the lakeside wetland nitrogen removal function.