The present invention relates to a bactericidal composition containing probenazole, which includes an active ingredient A and an active ingredient B, wherein the active ingredient A is probenazole, the active component B is one selected from the group consisting of carbendazim, thiophanate-methyl, fosetyl-aluminum, prochloraz, mancozeb, propineb, and polyram, and the weight ratio of the active component A and B is 1:80 to 80:1. The effective active ingredients are added with additives to prepare a wettable powder, a water dispersible granule, a suspending agent or a suspoemulsion. The composition is used for the control of rice blast, bacterial leaf blight, sheath blight, anthracnose, ring rot, brown spot disease, leaf spot disease, gibberellic disease, downy mildew, epidemic disease, powdery mildew, bakanae disease, penicilliosis, early blight, late blight, citrus scab, alternaria leaf spot, rust disease and scab, is resistant to rain wash and has obvious synergism.