33 results about "Bakanae" patented technology

The invention discloses a bactericidal composition containing WML-01 and a triazole compound, and is characterized in that the bactericidal composition is composed of the WML-01 and the triazole compound, and the mass ratio of the WML-01 to the triazole compound is (10: 1)-(1: 10). The bactericidal composition taking the WML-01 and the triazole medicament as effective components has a synergistic effect on prevention and treatment of rice bakanae disease, wheat stem rot, wheat scab, plant root rot and sclerotinia rot of colza, can effectively improve the disease prevention and treatment effect, reduces the pesticide consumption, delays the occurrence of drug resistance, improves the crop yield, and has a good application prospect. The method is safe, effective and economical, and has a wide market application prospect.

The invention discloses a streptomyces rimosus strain and application thereof, and belongs to the field of development and utilization of microbial germplasm resources, the strain is separated from a rice seedling bed rice sample in Wei village town in Xiaoshan area of Hangzhou City in Zhejiang Province, is classified and named as streptomyces rimosus, has a strain number of ZJU55, is preserved in China Center for Type Culture Collection (CCTCC) on November 22, 2024, and has a preservation number of CCTCC M 20242624. The streptomyces rimosus ZJU55 strain provided by the invention has an efficient antagonistic effect on fusarium ramosissimum, and a biocontrol inoculant prepared from fermentation liquor of the streptomyces rimosissimum ZJU55 strain can effectively inhibit the growth of fusarium ramosissimum hyphae, the production of spores and the germination of the spores, and can be used for preventing and treating rice bakanae disease caused by fusarium ramosissimum infection; the prevention and control efficiency of a pot experiment is stabilized to be 80% or above.

The invention discloses a rice bakanae disease prevention and treatment medicine and a preparation method thereof, and belongs to the technical field of agricultural disease prevention and treatment. The medicine for preventing and treating the rice bakanae disease is prepared from the following raw materials in parts by mass: 4 to 8 parts of cyrtomium rhizome extract, 0.5 to 1.5 parts of pydiflumetofen hydroxylamine, 0.1 to 0.5 part of isoxazole formic acid, 0.01 to 0.1 part of compound sodium nitrophenolate, 1 to 2 parts of surfactant, 0.1 to 2 parts of dispersant, 0.1 to 2 parts of stabilizer, 0.1 to 1 part of antioxidant and 90 to 100 parts of carrier. Through the synergistic effect of the cyrtomium fortunei extract, the pydiflumetofen and the isoxadifen carboxylic acid with different action mechanisms, the rice bakanae disease morbidity is remarkably reduced in a mode of simultaneously inhibiting growth and reproduction of pathogenic bacteria and transduction of a plant spindling signal, and the generation of resistance of the pathogenic bacteria is delayed.

This invention provides compounds and methods of application that possess both broad-spectrum antifungal activity and plant-induced resistance, belonging to the field of agricultural technology. It addresses the problem that "traditional pure immune inducers must be applied before disease onset, making it difficult to meet the full-cycle control needs of disease prevention and treatment in the field." Compared to the previously developed PC1 and PC20, the two compounds of this invention, 2,4-dibromo-6-nitrophenol and 7-deazon-2'-deoxy-7-iodoadenosine, can both inhibit the growth of rice blast fungus and significantly improve rice resistance to rice blast through foliar spraying, meeting the full-cycle control needs of disease prevention and treatment in the field. Furthermore, these two compounds can directly inhibit the growth of various fungal pathogens such as peanut white mold, tomato gray mold, wheat scab, rice bakanae disease, rice false smut, mango malformation, and banana wilt.

The invention discloses a bactericidal composition containing WML-01 and JS399-19, the active ingredients of the bactericidal composition comprise the WML-01 and the JS399-19, and the mass ratio of the WML-01 to the JS399-19 is (10: 1)-(1: 10). The bactericidal composition can be used for preventing and treating rice bakanae disease, wheat stem rot, wheat scab and plant root rot, can effectively solve the problems of few disease prevention and treatment agents, unstable prevention effect, high prevention and treatment cost and drug resistance in current production, and has a wide market application prospect.

This invention discloses a fungicide composition containing WML-01 and triazole compounds. The fungicide composition is characterized by comprising WML-01 and triazole compounds in a mass ratio of 10:1 to 1:10. The fungicide composition provided by this invention, with WML-01 and triazole agents as active ingredients, exhibits a synergistic effect in the control of rice bakanae disease, wheat stem rot, wheat scab, plant root rot, and rapeseed sclerotinia stem rot. It can effectively improve disease control efficacy, reduce pesticide usage, delay the development of pesticide resistance, and increase crop yield. It is safe, effective, and economical, and has broad market application prospects.

The invention discloses a bactericidal composition containing WML-01 and fludioxonil, the active ingredients of the bactericidal composition comprise the WML-01 and the fludioxonil, and the mass ratio of the WML-01 to the fludioxonil is (10: 1)-(1: 10). The bactericidal composition can be used for preventing and treating rice bakanae disease, wheat stem rot, wheat scab, soybean root rot, rice blast, pepper anthracnose and sclerotinia rot of colza, can effectively solve the problems of few disease prevention and treatment agents, unstable prevention effect, high prevention and treatment cost and drug resistance in current production, and has a wide market application prospect.

The invention relates to a sulfonamide compound containing thiazole heterocycle as well as a preparation method and application of the sulfonamide compound, and belongs to the technical field of agricultural chemicals and bactericides. According to the invention, substituted sulfonyl chloride and unsubstituted or substituted aminoacetophenone are used as main raw materials, the thiazole heterocyclic ring and a sulfonamide group are effectively combined through different reactions to prepare the novel sulfonamide compound containing the thiazole heterocyclic ring, and the structural general formula is shown as a formula I or a formula II. The compound provided by the invention has bacteriostatic activity on multiple plant pathogenic fungi such as sclerotinia sclerotiorum, rhizoctonia solani, botrytis cinerea, curvularia maydis, fusarium oxysporum, pyricularia grisea and the like, and an innovative solution is provided for agricultural sustainable disease prevention and control.

The invention discloses a seed treatment agent containing metalaxyl-M (A), benziothiazolinone (B) and pydiflumetofen (C) and application of the seed treatment agent. The mass ratio of the metalaxyl-M to the benziothiazolinone to the pydiflumetofen is (1-10): (1-5): (1-10). Secondly, the three effective components are compounded and prepared into the seed treatment suspending agent, and the seed treatment suspending agent can effectively prevent and treat rice bakanae disease, rice seedling rot, peanut damping-off and corn stalk rot. Through synergistic interaction of the three components, the bactericidal spectrum is effectively expanded, and the emergence rate of rice, peanut and corn seeds and the stress resistance of crops can be greatly improved, so that the yield is improved.

The invention provides a composition containing fluopyram, prothioconazole and amino-oligosaccharin. The composition is prepared from the following components in percentage by weight: 1 to 30 percent of fluopyram, 1 to 20 percent of prothioconazole and 0.1 to 5 percent of amino-oligosaccharin. The composition can be prepared into a suspended seed coating agent and a seed treatment suspending agent, and can be used for treating the two diseases, namely the rice bakanae disease and the rice aphelenchoides besseyi.

The invention relates to a microbial seed treatment agent and application thereof in agricultural production, and belongs to the field of research and development of agricultural microbial products. The bacillus Bs916 has a relatively strong effect of inhibiting pathogenic bacteria of the rice bakanae disease. The invention provides a seed treatment agent which is prepared by taking Bs916 living spores as active ingredients and mixing auxiliaries, the seed treatment agent is red suspension liquid, the spore content is more than or equal to 3 * 10 < 9 > CFU / mL, the suspension rate is more than 80%, and the pH is 6.0-7.0. The bacillus Bs916 seed treating agent has the effects of promoting rice seed germination and seedling growth and preventing and treating rice bakanae disease. When the seed treatment agent is respectively mixed with tebuconazole (250mg / L), validamycin (50mg / L) and agricultural resistance 120 (200mg / L), the effects of preventing and treating the rice bakanae disease are respectively 87.54 percent, 86.74 percent and 88.05 percent. The invention discloses a microbial seed treatment agent capable of promoting growth and preventing and treating rice bakanae disease, which can stabilize the prevention effect, reduce the dosage of chemical pesticide and reduce pesticide residue when being mixed with chemical pesticide, and has potential application value and market development prospect in agricultural production.

The invention discloses an application of a bactericidal agricultural composition containing WML-01 and a methoxy acrylate medicament (QoIs) in prevention and treatment of rice bakanae disease, wheat stem rot, wheat scab, plant root rot, rice blast, plant anthracnose and sclerotinia rot of colza. In the bactericidal agricultural composition containing the WML-01 and the methoxyl acrylate medicament, the mass ratio of the WML-01 to the methoxyl acrylate medicament is (10: 1) to (1: 10). The bactericidal agricultural composition can be used for preventing and treating rice bakanae disease, wheat stem rot, wheat scab, plant root rot, rice blast, plant anthracnose and sclerotinia rot of colza, and can effectively solve the problems of few medicaments for preventing and treating the seven diseases, unstable prevention effect, high prevention and treatment cost and drug resistance in the current production; wide market application prospects are realized.

The invention discloses a paenibacillus polymyxa strain and application thereof, and belongs to the field of development and utilization of microbial germplasm resources, the strain is separated from a rice seedling bed rice sample in Wei village town in Xiaoshan area of Hangzhou City in Zhejiang Province, is classified and named as paenibacillus polymyxa, has a strain number of ZJU66, is preserved in China Center for Type Culture Collection on May 26th, 2025, and has a preservation number of CGMCC NO.9687. The preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M 20251176. The paenibacillus polymyxa ZJU66 strain provided by the invention has an efficient antagonistic effect on wild type strains and prochloraz-resistant strains of the fusarium moniliforme, and fermentation liquor of the paenibacillus polymyxa ZJU66 strain can effectively inhibit mycelial growth, spore production and spore germination of the fusarium moniliforme. Moreover, the biocontrol microbial inoculum with the concentration of 106 CFU / mL prepared from the fermentation liquor can effectively prevent and treat the rice bakanae disease caused by fusarium moniliforme infection in a greenhouse test and a field test, the greenhouse control effect reaches 90% or above, and the field control effect reaches 80% or above.

The application discloses application of a rice OsPIP2;2 gene in improvement of seedling stage resistance to bakanae disease, a nucleotide sequence of the OsPIP2;2 gene is shown as SEQ ID NO. 1, and the OsPIP2;2 gene has a positive regulation effect on the resistance of rice to the bakanae disease. The application constructs mutants Ospip2;2-1 and Ospip2;2-2 with 93-11 material as a genetic background by using a gene editing technology, and phenotype identification is carried out seven days after inoculation of bakanae disease bacteria, and the result shows that the relative seedling length of the Ospip2;2-1 and Ospip2;2-2 mutants is significantly higher than that of a wild type; OsPIP2;2 overexpression lines OE-1 and OE-2 with Nipponbare as a genetic background are obtained by using an agrobacterium-mediated genetic transformation, and phenotype identification is carried out seven days after inoculation of bakanae disease bacteria, and the result shows that the relative seedling length of the OsPIP2;2 overexpression lines OE-1 and OE-2 is significantly lower than that of the wild type. In summary, the overexpression line of the rice OsPIP2;2 gene has better resistance to the bakanae disease than the mutant of the rice OsPIP2;2 gene, that is, the OsPIP2;2 gene has a positive regulation effect on the resistance of rice to the bakanae disease.

The present invention discloses a method and system for detecting the type of Bakanae nematodes in rice seeds, relating to the field of rapid rice seed quality detection. The method comprises: inputting a hyperspectral image of the rice seeds to be detected into a rice seed Bakanae nematodes type detection model to determine the detection result of the rice seeds; the detection result is the absence of Bakanae nematodes or the type of Bakanae nematodes; wherein the rice seed Bakanae nematodes type detection model is determined based on an activation wavelength and an original deep convolutional neural network; the activation wavelength is the wavelength activated when the trained deep convolutional neural network correctly classifies; the trained deep convolutional neural network is a neural network obtained by training the original deep convolutional neural network using a training set. The present invention can quickly and accurately detect the type of Bakanae nematodes carried in rice seeds.

The invention discloses a molecular marker screening method of a rice bakanae disease resistance gene, and relates to the field of biotechnology and plant genetic breeding. The method comprises the following components: S1, construction of a rice protein library and phage display, S2, specific binding screening with pathogenic bacteria of the rice bakanae disease, S3, identification of resistance proteins and corresponding genes, and S4, development of molecular markers. By constructing a rice protein library phage display system and utilizing a specific binding screening technology with rice bakanae disease pathogenic bacteria, the resistance-related protein and the corresponding gene can be efficiently screened from a large number of rice genes, and compared with a traditional method, the screening mode is more direct and efficient, and the screening cost is reduced. Errors caused by non-specific screening are reduced, and the efficiency and accuracy of resistance gene screening are greatly improved.

The invention belongs to the technical field of pesticides, and particularly relates to a bactericide composition containing pydiflumetofen and benzovindiflupyr. The invention relates to a bactericide composition. The effective components of the bactericide composition are formed by compounding pydiflumetofen and triflumizole allylbenzene. According to the bactericidal composition disclosed by the invention, the pydiflumetofen and the triflumizole allylbenzene are compounded according to a certain mass, so that the bactericidal composition shows a synergistic interaction effect on various plant fungal disease pathogenic bacteria, and the control effect on plant diseases can be improved; the composition can provide support for prevention and treatment of wheat sharp eyespot, wheat leaf spot, wheat root rot, curvularia maize leaf spot, corn southern leaf blight and rice bakanae disease.

The invention discloses a method for constructing an RNA interference vector of pathogenic genes of rice bakanae disease pathogenic bacteria, and relates to the technical field of genetic engineering.The construction method comprises the steps that pathogenic gene segments are obtained, specifically, the pathogenic gene sequences of the rice bakanae disease pathogenic bacteria are retrieved from a gene database, specific primers with specific restriction enzyme recognition sites are designed, and the specific primers are used for identifying the pathogenic genes of the rice bakanae disease pathogenic bacteria; the method comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification by taking pathogenic bacteria genome DNA as a template, and recovering and purifying a target gene segment after agarose gel electrophoresis separation; by optimizing the construction process of the RNA interference vector, efficient silencing of a target gene is achieved, specific restriction enzyme recognition sites and specific primers are utilized, pathogenic gene segments of pathogenic bacteria of the rice bakanae disease are retrieved and amplified from a gene database, and the pathogenic gene segments of the pathogenic bacteria of the rice bakanae disease are obtained. And then the RNA is connected and transformed with an intermediate vector and a plant expression vector to finally obtain the RNA interference vector capable of silencing the pathogenic gene, and the method has higher construction efficiency and more accurate silencing effect.

The present invention discloses a pesticide composition containing trichlorfonazole, bronopol and cartap and its application, comprising effective ingredients trichlorfonazole, bronopol and cartap, wherein the mass ratio of trichlorfonazole and bronopol with fungicidal activity to cartap with insecticidal activity is 1-10:1-10:1-10. The present invention also discloses the application of the pesticide composition in preventing and treating rice seedling rot or / and rice seedling rot or / and rice dry tip nematode disease or / and rice bacterial base rot. The active ingredients of the pesticide composition of the present invention have different action mechanisms, expand the prevention and control spectrum, and have significant synergistic synergy. The resistance of rice seedling pathogens to fungicides such as carbendazim, prochloraz and cyanothiocarb can be effectively controlled, and the resistance control of rice seedling rot in my country has great application value. It is effective and has significant efficiency in preventing and controlling rice seedling rot, rice seedling blight, rice stem tip nematode disease and rice bacterial base rot. It can reduce the use of pesticides, has high quality and low price, and is environmentally friendly and safe.

The invention relates to the technical field of rice seed treating agents, in particular to a rice seed treating agent and application thereof, the rice seed treating agent comprises an effective component A, an effective component B and an assistant, the effective component A is pydiflumetofen, the effective component B is ipconazole, the content of the component A is 1-9%, and the content of the component B is 3-20%. According to the invention, the pydiflumetofen and ipconazole are compounded, so that the safety of rice seed treatment is effectively improved, a very good prevention and treatment effect on bakanae disease germs carried on rice seeds is achieved, the prevention and treatment effect is outstanding, the germination rate of the seeds can be effectively ensured in a seed germination accelerating process, the emergence rate is excellent, and the application prospect is wide. The germination probability of bakanae disease germs is greatly reduced, and a very good prevention effect on the rice bakanae disease is achieved. The medicament disclosed by the invention is applied to prevention and treatment of the rice bakanae disease, effectively plays a good role in preventing the rice bakanae disease, is long in prevention and treatment effect time, causes few bakanae disease after growth and development of the rice, and greatly reduces the harm of the bakanae disease to the rice.

The invention belongs to the technical field of microorganisms, and particularly relates to a biocontrol bacterium DHR14 for preventing and treating red root diseases of rubber trees and application of the biocontrol bacterium DHR14. The biocontrol bacterium DHR14 for preventing and treating the hevea brasiliensis has broad-spectrum antibacterial activity, has a good antagonistic effect on various pathogenic bacteria such as hevea brasiliensis and the like, and is a potential biological agent for preventing and treating the hevea brasiliensis, rice bakanae disease, eggplant fusarium wilt and the like; in addition, the biocontrol bacterium for the red root disease of the rubber tree also has good capabilities of producing protease, protease, cellulase, beta-glucanase, amylase, siderophore and the like. The biocontrol bacterium DHR14 for preventing and treating the hevea brasiliensis has a wide development space, and a new candidate strain is provided for biological prevention and treatment of the hevea brasiliensis, rhizoctonia solani, fusarium moniliforme and other pathogenic bacteria.

The invention discloses an antagonistic bacillus compound preparation and application of the antagonistic bacillus compound preparation to prevention and treatment of rice bakanae disease, and relates to the technical field of agricultural biological prevention and treatment. By combining the magnetic nanoparticles and applying the specific magnetic field, the composite preparation can be guided to gather towards the root system and the disease-prone part of the rice, and by means of the precise targeted prevention and control mode, the prevention and control effect of the composite preparation on the rice bakanae disease is remarkably improved, and the problems of agent waste and environmental pollution possibly existing in a traditional prevention and control method are reduced.

The invention provides a system, a kit and a method for detecting fusarium moniliforme of fusarium moniliforme of rice fusarium moniliforme. The system can accurately, quickly and efficiently detect weak positive and suspected positive samples with extremely low content of fusarium moniliforme of fusarium moniliforme of rice fusarium moniliforme, and has relatively high sensitivity and specificity, and the sensitivity can reach 1 copy / mu L and is far beyond the lower detection limit in the prior art. Therefore, the method can detect the fusarium moniliforme in early rice seeds, so that the rice bakanae disease is prevented.

The pesticide spraying device comprises a base, a box body and a fixing seat, one end of the top of the base is connected with the box body, and the other end of the top of the base is connected with the fixing seat; and one side of the box body is connected with a servo motor, one side of the servo motor is connected with a connecting rod, the outer portion of the connecting rod is evenly sleeved with sleeve blocks, and the two ends of each sleeve block are each provided with a rotating blade. The lifting rod is adjusted to the needed height by pulling the lifting rod outwards, then the lifting rod is aligned with the round hole in the stand column, the stand column and the lifting rod are fixed by inserting the positioning pin into the round hole, and due to the fact that the conveying pipe is composed of the straight pipe and the corrugated pipe together, when the lifting rod ascends, the spray head ascends, and the spray head is fixed. The height of the pesticide spraying device can be conveniently adjusted according to the growth cycle of rice, and the rice bakanae disease can be prevented and controlled.

The disclosure belongs to the technical field of rice planting and discloses a method for raising rice seedlings in block matrix cotton based on a walnut green peel fermentation extract. The walnut green peel fermentation extract of the disclosure is obtained by crushing and fermenting the walnut green peel, decomposing it in an alkaline environment, and extracting a water-soluble extract and an organic solvent extract, respectively. The obtained walnut green peel fermentation extract can improve the effect in soaking seeds of rice, adjust an acidity of the matrix cotton, and synergistically improve prevention and control of seed-borne diseases such as bakanae disease; and the walnut green peel fermentation extract is used to replace chemical control agents in block matrix cotton seedling raising to promote strong seedlings and root growth, which facilitates machine transplantation and improves machine transplantation efficiency.

The invention belongs to the technical field of microorganisms, and particularly relates to a rubber tree red root disease biocontrol bacterium QHL1 and application thereof. The biocontrol bacterium QHL1 for the red root disease of the rubber tree, disclosed by the invention, has broad-spectrum bacteriostatic activity, and has a good antagonistic effect on red root pathogen of the rubber tree, rhizoctonia solani, fusarium moniliforme, eggplant fusarium wilt pathogen, alternaria solani, fusarium graminearum, wheat root rot pathogen, phytophthora parasitica var nicotianae, alternaria alternata, bipolaris maydis and the like; the biocontrol bacterium is a potential biological agent for preventing and treating hevea brasiliensis red root diseases, rice bakanae diseases, eggplant fusarium wilt and the like, and also has good capabilities of producing protease, cellulase, beta-glucanase, amylase, siderophore and the like. The biocontrol bacterium QHL1 for the red root disease of the rubber tree has a wide development space and a good development and application prospect, and provides a candidate resource for prevention and treatment of the red root disease of the rubber tree and other plant diseases.

The present invention relates to the field of pesticides and fungicides, and specifically discloses a pesticide composition containing prothioconazole. The active ingredients of the pesticide composition include a fungicide and an insecticide, wherein the fungicide includes prothioconazole and trifloxystrobin, and the insecticide is thiamethoxam. The mass ratio of the fungicide prothioconazole to trifloxystrobin is 1:10 to 10:1, and the mass ratio of the fungicide to the insecticide is 1:1 to 5:1. The pesticide composition of the present invention can treat both rice diseases and insect pests, and has a particularly good synergistic effect against rice bakanae disease and rice thrips. A single application of the pesticide provides significant control efficacy, reduces the amount of pesticide used, and reduces toxicity.

The invention discloses a composite microbial inoculant for preventing and treating rice bakanae disease as well as a preparation method and application of the composite microbial inoculant, the composite microbial inoculant is composed of a fungal hypha suspension of plant endophytic fungi phomopsis sp. And a bacterial suspension formed by mixing pseudomonas otitis media BF72 and bacillus subtilis BF86 according to an equal proportion; the amount (dry weight) of fungal hyphae in the composite bacterial agent is 0.01 g / 5ml, and the effective viable count of the composite bacterial suspension is 1 * 10 < 8 >-10 < 9 > CFU. The composite microbial agent screened, combined and constructed by the invention has an obvious inhibition effect on the occurrence of the rice bakanae disease, improves the growth state of diseased rice, reduces the abundance of pathogenic bacteria in the rice, and improves the POD, SOD and PAL activities of the overground part and the underground part of the rice, so that the resistance of the rice to the pathogenic bacteria is enhanced, and the control effect of the composite microbial agent is superior to that of a chemical agent. The method is low in operation cost, has universality and is easy for large-scale production.

The present invention provides a seed treatment suspension agent, wherein the active ingredients are composed of hexaconazole and fludioxonil, and the mass ratio of hexaconazole to fludioxonil is 3:1 - 1:7. The weight percentage content of the active ingredients is 1 - 10%; it also contains the following excipients in weight percentage: 3 - 18% of a wetting and dispersing agent, 0.5 - 5% of a thickening agent, 1 - 6% of a film-forming agent, 1 - 5% of a warning agent, 1 - 8% of an antifreezing agent, 0.1 - 1.5% of a preservative, and water makes up to 100%. The seed treatment suspension agent provided by the present invention has excellent synergistic effects and formulation properties, can be used for the prevention and control of bakanae disease of rice, reduce the dosage of pesticides, and slow down the generation of drug resistance.

The present invention relates to an asymmetric aromatic diselenide compound and the use thereof in the preparation of pesticides, particularly to the use thereof in the preparation of anti-plant virus agents and fungicides. The asymmetric aromatic diselenide compound of the present invention exhibits a good efficiency on tobacco mosaic virus (TMV) at a concentration of 500 mg / L, achieving an in-vivo inactivation efficiency of 71.6%, an in-vivo protective efficiency of 58.3%, and an in-vivo therapeutic efficiency of 60.1%. The asymmetric aromatic diselenide compound of the present invention exhibits good control effects on tomato early blight, wheat Fusarium head blight, rice blast, pepper Phytophthora blight, rapeseed Sclerotinia rot, cucumber gray mold, rice sheath blight, cucumber wilt, peanut brown spot, apple ring rot, wheat sharp eyespot, southern corn leaf blight, watermelon anthracnose, and rice bakanae disease at a concentration of 50 mg / L, and has a fungicidal activity of up to 92.3%.