Pseudomonas mosselii and application thereof

A technology of pseudomonas and engineering bacteria, applied in the field of pseudomonas, can solve problems such as environmental and ecological pollution and harm

Active Publication Date: 2016-09-07
杭州摩速科学器材有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical control is currently the most effective measure to prevent fungal diseases. The annual use of chemical pesticides in my country is 500,000-600,000 tons, which has brought serious pollution and harm to the environment and ecology. In some countries and regions, chemical fungicides have been restricted. use

Method used

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  • Pseudomonas mosselii and application thereof
  • Pseudomonas mosselii and application thereof
  • Pseudomonas mosselii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Collection of soil samples

[0033] Soil samples were randomly collected from Taoyuan Mountain in Lijiang, Yunnan. Remove the surface soil, collect about 300g of soil samples at a depth of 5-20cm, and bring them back to the laboratory after subpackaging and marking.

[0034] 2. Isolation of strains

[0035] Separate by soil dilution method, weigh 10g of soil and pour it into a 90ml sterile water triangular flask equipped with glass beads, this is 10 -1 After shaking for 10 minutes, draw 1ml into a new triangular flask filled with 90ml of sterile water, shake and mix well, this is 10 -2 The diluent, according to this method continue to dilute to 10 -4 , 10 -5 , 10 -6 diluent. draw 10 -4 、10 -5 and 10 -6 Add 100 μl of the diluent to the KB solid medium plate, repeat each gradient 3 times, spread evenly, place it upside down in a 28°C incubator and cultivate it for 24 hours, then streak and purify typical colonies on the KB medium plate for 2-3 times A single ...

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Abstract

The invention relates to the technical field of pseudomonas mosselii, in particular to pseudomonas mosselii. The pseudomonas mosselii is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No. 12222. The pseudomonas mosselii has an antibacterial effect on pathogenic bacteria of rice blast, rice bakanae disease, rice sheath blight disease, rice seedling blight and rice false smut.

Description

technical field [0001] The invention relates to the technical field of Pseudomonas, in particular to Pseudomonas mosselii. Background technique [0002] Fungal diseases seriously endanger global food security, and 70%-80% of plant diseases are caused by the infection of pathogenic fungi. Rice blast (Magnaporthe oryzae) broke out in 85 countries around the world, causing 10%-35% grain loss, and even some fungal diseases have also caused serious death and extinction of animals and plants. Chemical control is currently the most effective measure to prevent fungal diseases. The annual use of chemical pesticides in my country is 500,000-600,000 tons, which has brought serious pollution and harm to the environment and ecology. In some countries and regions, chemical fungicides have been restricted. use. Biological control has broad application prospects because of its quick effect, wide bactericidal spectrum, environmental friendliness and resistance to resistance. [0003] The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/21A01N63/00A01P3/00C12R1/38
CPCA01N63/00C12N1/205C12R2001/38
Inventor 冯国忠吴丽娟陈国庆严清
Owner 杭州摩速科学器材有限公司
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