141results about How to "Inhibit pathogenic bacteria" patented technology

The invention discloses enterococcus faecium and a preparation method thereof, relates to the field of microorganisms, and particularly relates to the enterococcus faecium and application thereof. A bacterium sample is acquired from the rumen of a slaughtered goat, subjected to directive breeding at the temperature of 32-42 DEG C under an anaerobic and photophobic condition, and separated and purified by use of a repeated divisional agar streak plate method so as to obtain enterococcus faecium L-01. The enterococcus faecium L-01 can be used as an animal microorganism fodder additive, has the effects of effectively inhibiting pathogenic bacterium in animal digestive tract, improving the micro-ecological environment of the digestive tract and enhancing the immunologic function of an animal body, can be used as a substitute product of antibiotics and can be used for effectively solving the existing serious situation.

The invention relates to a high-density culture method of bifidobacterium lactis V9 and a preparation method of a freeze-dried powder of the bifidobacterium lactis V9, and belongs to the field of probiotics fermentation. The bifidobacterium lactis V9 (Bifidobacterium animalis subsp.lactis V9) is acid-resistant and cholate-resistant probiotics which are separated from the intestinal tract of a healthy Mongolian child, and the collection number of the bifidobacterium lactis V9 is CGMCC No.5470. High-density fermentation of the bacteria of the bifidobacterium lactis V9 is realized under the control of an optimized culture medium and optimized fermentation conditions; the viable count in a fermentation liquor can be more than 2.0*10<10>cfu/ml, and the viable count in the freeze-dried powder of the bifidobacterium lactis prepared by using the fermentation liquor can be more than 6.0*10<11>cfu/g, and therefore, the freeze-dried powder of the bifidobacterium lactis can be applied to a probiotic leavening agent or a probiotic preparation.

The invention provides a piglet milk powder capable of completely replacing sow milk, comprising the following components: 25-98wt% of dried whole milk, 0-70wt% of defatted milk powder, 0-13wt% of dried egg powder, 0-30wt% of mixed powder of emulsified plant fat and lactose, 0-5wt% of whey powder, 0.5-2.0wt% of lactocin powder, 0.3wt% of probiotics preparation, 0.2-1.0wt% of acidulant, 0.15-0.3wt% of trace elements pre-mix material, 0.25-1.0g/kg of piglet vitamin pre-mix material, 50-300mg/kg of vitamin C, 800-2000mg/kg of choline chloride, 10-50mg/kg of colistine sulfate, and 30-80mg/kg of oxycycline; and the rest is flavoring additive and preservative.

The present invention belongs to the field of microbial technology, relates to a liquid-solid combined fermentation process of probiotics using bifidobacterium as main raw material, mainly includes the following steps: making bifidobacterium, acidophilic lactobacillus and thermophilic streptococcus respectively undergo the two stages of stock 1-3 order liquid culture and solid culture, the weightratio of three bacteria powders in the preparation is 50-80:10-15:5-16. Said solid culture includes the following procedures: firstly, sterilizing, inoculating 20-30% of tertiary culture, placing them into plastic bag, vacuum-pumping, injection anaerobic sterile N2 into said non-toxic sterile plastic bag, and adding bifiobacterium into solid culture medium, and promoting live bacterium multiplication to 10 to the power 8-10 to the power 9 order, and can prolong its storage time.

The invention belongs to the technical field of microorganisms, and particularly relates to a composite efficient microbial deodorizer and a preparation method thereof. The microbial deodorizer comprises candida utilis, bacillus subtilis, bacillus licheniformis, bacillus coagulans and enterococcus lactobacilli, viable count is 5.3*10<9>-8.3*10<9>cfu/g, viable count of the candida utilis is 1.8*10<8>-3.6*10<8>cfu/g, viable count of the bacillus subtilis is 3.4*10<8>-4.2*10<8>cfu/g, viable count of the bacillus licheniformis is 3.7*10<8>-7.4*10<8>cfu/g, viable count of the bacillus coagulans is 2.4*10<8>-3.6*10<8>cfu/g, and viable count of the enterococcus lactobacilli is 4.2*10<9>-6.4*10<9>cfu/g. The microbial deodorizer has strange deodorization effects and can rapidly and effectively remove odorous gas of pollution sources.

The invention relates to the field of agricultural cultivation, and relates to a paddy rice cultivation method. The method comprises the following steps: (1) paddy rice seeds are selected, and are subjected to seed soaking for 12-16h with a seed soaking liquid under a temperature of 35-37 DEG C; (2) a seedling-stage fertilizer is sprayed, and a seedling-stage substrate is applied; (2) when the seedlings reach a stage of 5-6 leaves, the seedlings are transplanted into a field spread with a cultivation base fertilizer; (4) when the paddy rice seedlings start tillering, a tillering fertilizer is applied; (5) when the paddy rice seedlings starts flowering, a flowering-stage fertilizer is applied; (6) when the paddy rice seedlings starts filling, a filling fertilizer is applied, and a foliar fertilizer is added; and (7) harvesting is carried out. The method provided by the invention is highly targeting. Targeting fertilization is carried out during various growth periods. With the treatment with the seed soaking liquid, a germination period can be shortened, paddy rice growth can be accelerated, paddy rice stress resistance can be improved, a period from germination to flowering can be shortened, and a flowering period and a fruiting period can be prolonged. During the tillering stage, tillering amount is increased with the tillering fertilizer. With the flowering-stage fertilizer applied during the flowering stage, pollination success rate can be improved. During the filling stage, with the filling fertilizer and the foliar fertilizer, filling rate is greatly improved, yield is improved, and paddy rice grain fullness is improved.

The invention relates to a preparing method and application of a composite biological agent for agricultural root-knot nematodes. The biological agent is obtained through solid fermentation of paecilomyces lilacinus YT08 with the preservation number of CG MCC No.10026 and has a powerful inhibitory effect on the agricultural root-knot nematode disease. A preferred culture medium involved in solid fermentation is prepared from 17-30% of dried brewer grain, 4-8% of bran, 3-6% of smashed peanut shells, 2-5% of soy peptone, 2.55-7.5% of powder chitosan (with the deacetylation degree larger than or equal to 90%), 1-2.25% of crab shell meal, 0.2-0.5% of NaCl, 0.1-0.3% of CaCl2, 0.1-0.3% of MgSO4, 0.05-0.15% of FeSO4 and 40-70% of water. The preparing method has the advantages of being easy to operate and control and suitable for industrial production, the raw materials are low in cost, and the composite biological agent has the powerful effect.

The present invention discloses a kefir acid soymilk and a production method thereof. The kefir acid soymilk consists of soybean milk and animal milk which are matched to take effect of the complementation of protein nutrition. The acid soymilk which is produced by fermenting the soybean milk through microorganism not only has the nutrient efficacy of the soybean, but the microorganism fermenting process is also good for eliminating anti-nutritional factors in the soybean, thus improving the nutrient and healthy efficacy on the soybean milk. The kefir acid soymilk has smooth taste, proper acidity, soft flavor and intense bee wine fragrance, besides, aromatic carbonyl compound, phenolic acid substance and the like in the soybean take certain restraining effect on partial microorganism in the kefir. Compared with the traditional kefir, the kefir acid soymilk has the low excitation of the taste, is fit for the taste of most people and reduces the post acidification during storage greatly, and the shelf life is prolonged. The preparation method has low cost, simple operation and high feasibility and has good application prospect.

The invention discloses a holothurian culture water quality restoration agent for effectively removing water eutrophication and a preparation method and application of the holothurian culture water quality restoration agent, belonging to the technical field of aquaculture and marine microorganisms. Aiming at the physiological habit and culture characteristic of holothurian, a marine microorganism technology is applied, the restoration agent consists of bacillus flexus CGMCC No.5115, bacillus subtilis CGMCC No.5116 and bacillus cereus CGMCC No.5117 separated and screened from a holothurian culture environment, corn meal and soya bean meal serving as major culture raw materials are fermented at the temperature of 28-37 DEG C for 24-48 hours, and attapulgite, medical stone and rice hull serving as adsorption and suspension composite carriers are used for processing to obtain the holothurian culture water quality restoration agent for effectively removing water eutrophication. The product can adsorb harmful substances such as heavy ions including Hg<2+>, Pb<2+>, Cr<6+>, Cd<2+>, and Cu<2+> in water bodies, and the content of harmful substances such as ammonia nitrogen, nitrite, and hydrogen sulfide can be greatly reduced. Bottom odor and acidification of water bodies are avoided, pathogenic microorganisms are inhibited effectively, holothurian diseases are prevented, and the holothurian culture yield and quality are improved.

The invention belongs to the technical field of aquaculture and discloses a compounded oxygen producer for removing nitrite in aquatic water and a preparation method. The oxygen producer is prepared from components in parts by weight as follows: 40-50 parts of oxygen production ingredients, 24-38 parts of a compound microbial agent, 5-29 parts of an adsorbent, 1.0-2.5 parts of a growth accelerant, 7-10 parts of a traditional Chinese medicine admixture, 0.02-0.12 parts of a synergistic agent, 1-3 parts of a stabilizer and 1.4-2.2 parts of a sustained-release agent; the compound microbial agent comprises denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, bacillus laterosporus, bacillus subtilis, rhodococcus erythropolis, silicate bacteria, streptococcus faecalis and bacillus coagulans. Multiple microbial agents are compounded in the oxygen producer, and the compounded oxygen producer can effectively remove nitrite, has the functions of inhibiting pathogenic bacteria in the aquatic water and improving digestion of intestinal tracts of aquatic animals and has better popularization and application value.

The invention discloses a microbial agent for a soil conditioner. The microbial agent is composed of the following components: 1.0 to 3.0 * 1010 CFU/gram of Bacillus subtilis, 4.0 to 6.0 * 108 CFU/gram of Paecilomyces lilacinus, 2.0 to 4.0 * 1010 CFU/gram of Bacillus licheniformis, 4.0 to 6.0 * 108 CFU/gram of Bacillus mucilaginosus, 1.0 to 2.0 * 109 CFU/gram of Bacillus megaterium, 100 to 300 milligram/gram of gamma-polyglutamic acid and 500 to 600 milligram/gram of a carrier. The microbial agent provided by the invention can inhibit soil pathogens and loosen soil through passivation and adsorption of heavy metals, so the purposes of eliminating soil hardening, lightening moss and green moss, mitigating the harm of the heavy metals, purifying the soil and improving the yield and the quality of crops are achieved.

The invention discloses a creep feed capable of enhancing immunity of piglets and a preparation method thereof. The creep feed comprises the following components in parts by weight: 20 to 40 parts ofcorn, 5 to 15 parts of puffed corn; 5 to 15 parts of whey powder; 5 to 10 parts of puffed soybeans; 5 to 15 parts of soybean meal, 1 to 5 parts of fermented soybean meal; 5 to 10 parts of skim milk powder; 1 to 5 parts of sucrose, 1 to 5 parts of fish meal, 0.5 to 2 parts of soybean oil, 0.1 to 0.2 part of tributyrin; 1 to 1.5 parts of an organic compound acidifier; 0.5 to 1.0 part of an organic mineral substance premixing agent; 0.02 to 0.05 part of a vitamin premix; 0.05 to 0.2 part of a compound enzyme preparation; 0.1 to 0.2 part of a composite micro-ecological preparation; 0.1 to 0.5 partof oligosaccharide, 0.01 to 0.05 part of plant essential oil, 0.1 to 0.8 part of stone powder, 0.6 to 1.5 parts of calcium hydrophosphate, 0.1 to 0.4 part of table salt, 0.6 to 1.5 parts of compoundamino acid, 0.05 to 0.3 part of choline chloride and 0.03 to 0.08 part of a mildew preventive; the cfeed can promote ingestion of the piglets, guarantee normal physiological functions of organisms, promote development of immune organs, improve immunity of the piglets, enhance disease resistance and reduce diarrhea of the weaned piglets.

The invention discloses a solid beverage capable of improving the health problem of women. The solid beverage comprises the following active ingredients in percentages by weight: 30% to 35% of probiotics powder and 4% to 6% of cranberry extract, and further comprises the following auxiliary materials in percentages by weight: 50% of fructo-oligosaccharide, 10% to 12% of xylitol and 0.35% of microcrystalline cellulose. The solid beverage disclosed by the invention uses a pure biotherapy method matched with conventional therapy, so that the cure rate of disease treatment is effectively improved, and the recurrence rate is reduced. Meanwhile, the health of human bodies is maintained after diseases are treated in a natural development state, so that the women are get rid of the trouble of various women diseases, and the health life quality of the women is improved.

The invention provides a planting method for loofah. The planting method comprises the following concrete steps: soil improvement, seed soaking treatment, sowing, management in a seedling period, and field management. The planting method for the loofah provided by the invention is green, environmentally friendly and pollution-free; the loofah has rapid growth speed, healthy and strong plants, and strong stress resistance; disease resistance of the loofah is reinforced, and growth is promoted; the yield and the quality of the loofah are improved; meanwhile, the cultivated loofah has rich nutrition, and fine, tender and fresh tastes.

A Chinese medicine for treating chronic pelvic inflammation is prepared from 10 Chinese-medicinal materials including astragalus root, pilose asiabell root, Chinese agnelica root, Chuan-xiong rhizome, etc. Its quality control method includes such steps as thin-layer discrimination to Chuan-xiong rhizome, Chinese angelica root, tree peony bark, astragalus root and red peony root, and measuring the content of astragalus root.

The invention discloses an anti-acne composition containing epidermal growth factors and tea tree oil and a preparation method thereof. The anti-acne composition disclosed by the invention comprises the following components: epidermal growth factors, tea tree oil, metallothionein, chlorhexidine, humectant, tackifier, alpha-carboxylic acid, pH value regulator, surfactant and deionized water. The preparation method of the anti-acne composition disclosed by the invention comprises the following steps of: mixing and heating the epidermal growth factors, the tea tree oil, the metallothionein, the chlorhexidine and the surfactant to be used as an oil phase; dissolving and heating the humectant, the tackifier and the alpha-carboxylic acid in water to be used as a water phase; adding the oil phase into the water phase, and stirring uniformly; and adding the pH value regulator for regulating the pH value, thereby obtaining the anti-acne composition containing epidermal growth factors and tea tree oil. The epidermal growth factors contained in the anti-acne composition disclosed by the invention can accelerate the metabolism of skin cells, and the natural tea tree oil capable of resisting bacteria and diminishing inflammation can efficiently sterilize bacteria, diminish inflammation and remove acnes, thus the anti-acne composition has good moisture retention and small skin irritation.

The invention relates to the field of agriculture soil, in particular to a method for producing a soil conditioner by using chrysanthemum endophytic fungi or probiotic. The method comprises the following steps: activating the chrysanthemum endophytic fungi or probiotic; culturing the chrysanthemum endophytic fungi or probiotic on a liquid culture medium which contains potato diffusion juice, chrysanthemum leaf diffusion juice and glucose, or a solid culture medium which contains chrysanthemum straws; adding a small amount of nutrient salt to the chrysanthemum endophytic fungi or probiotic; and soaking the roots when the chrysanthemum endophytic fungi or probiotic is transplanted or adding the chrysanthemum endophytic fungi or probiotic to rhizosphere soil directly. The production of the soil conditioner is not influenced by the environment, has short production period, simple production process, easily controlled production time and yield, and obvious effect. A method for soaking the root of a plant or irrigating with the soil conditioner and adding the soil-conditioner to soil directly is adopted so that the soil conditioner acts on the plant directly. The soil-conditioner has the advantages of simply method, easy operation, less usage, low cost, and good effect.

The invention discloses a microbial agent for promoting aerobic composting of dead pigs and an application of the microbial agent. The microbial agent includes bacillus licheniformis ZR-1, bacillus subtilis dcy-1, bacillus methylotrophicus F7 and bacillus amyloliquefaciens YZ-0001. The microbial agent beneficial to degradation of organic matter is inoculated in the beginning of composting, maximumtemperature of a composting body is increased, high-temperature phase of the composting body is prolonged, pathogenic microbes are inactivated, composting of dead bodies is fastened, degradation of plant toxicity is promoted, and seed germination index is increased; the microbial agent is low in cost, simple in composition, convenient in preparation and easy to promote and apply; the microbial agent of aerobic composting of the dead pigs can generate stable humus and can be used as organic fertilizer applied to crop planting, suppress phytopathogens and reduce use of the fertilizer, and gooddevelopment prospect is achieved.

The invention discloses a zasiokaurin-containing salad sauce and a preparation method thereof, which belong to the food processing field. The zasiokaurin-containing salad sauce comprises the following raw materials by weight: 10-30 parts of zasiokaurin, 40-60 parts of soybean powder, 30-60 parts of honey, 40-60 parts of milk powder, 20-50 parts of lemon juice, 40-60 parts of egg yolk, 80-130 parts of olive oil, 10-20 parts of matrimony vine powder, 10-20 parts of red date powder, 2-5 parts of salt, 1-3 parts of additive and 50-100 parts of cold water. The additive is ascorbic acid, protamine, propolis and tea polyphenol with weight ratio being 1: 2: 3: 2. The zasiokaurin-containing salad sauce is prepared by taking zasiokaurin, matrimony vine and red date powder as raw materials, the zasiokaurin-containing salad sauce has health care function as a food flavoring agent, and the preparation method also solves the difficult wall breaking of zasiokaurin and difficult absorption.

The invention discloses a compound type antibiotic-free feed additive and belongs to the field of animal feed. The feed additive disclosed by the invention is mainly prepared from a compound microorganism, a compound enzyme preparation, a mineral substance, oligosaccharide and echinacea polyphenol (2-6) and an acidifier. The antibiotic-free feed additive disclosed by the invention can be used forreplacing an antibiotic additive and the bacterium-inhibition effect is realized; the antibiotic-free feed additive can have the functions of promoting digestion and absorption of feed, inhibiting pathogenic bacteria, preventing diarrhea, promoting intestine health of animals, enhancing the immunity of the animals, preventing diseases and improving the disease-resisting capability. The compound type antibiotic-free feed additive is mainly added into the feed to carry out antibiotic-free feed breeding.

The invention discloses lactobacillus gasseri LS03 and application thereof, and belongs to the technical field of microorganisms. The invention discloses a strain of lactobacillus gasseri LS03, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 22009. Lactobacillus gasseri LS03 can produce lactic acid to reduce the pH value; and meanwhile, the growth of pathogenic bacteria can be remarkably inhibited in vitro, the adhesion of escherichia coli, staphylococcus aureus and candida albicans to human vaginal epithelial cells VK2/E6E7 can be remarkably inhibited, and the lactobacillus gasseri LS03 can be used for preparing medicines for treating and/or preventing female genital tract diseases. The lactobacillus gasseri LS03 disclosed by the invention has a huge potential application prospect in the aspect of relieving and/or preventing female genital tract diseases.