Microbe bacteria agent for treatment of refinery waste water, its preparation method and application

[0037] Example 1:

[0038] Category

[0039] 2 Quantitatively prepare the solid medium after high temperature sterilization (121°C, 30 minutes). The formula of the solid medium is beef extract 0.5%, peptone 1%, NaCl 0.5%, water 100g, and agar 1.7%.

[0040] 3 Pre-prepared strains and inoculate the pre-prepared 14357, Na4, EM, CB4, PNCB3, 10-4 into the sterilized solid medium for activation.

[0041] 4 Quantitatively prepare the liquid medium that has been sterilized by high temperature (121° C., 30 minutes). The liquid medium formula is beef extract 0.5%, peptone 1%, NaCl 0.5%, and water 100g.

[0042] 5 Inoculate the activated strains into the liquid medium and shake (37°C, 160 revolutions/min) for 2 days.

[0043] 6 Count the number of the cultured bacterial liquid, and then combine the number ratio of 14357:Na4:CB1:PNCB3:10-4:EM=3:4:2:3:3:6 to obtain a mixed bacterial liquid.

[0044] 8 How to use: Add 5ml of activated sludge to the Erlenmeyer flask containing 100ml of sterilized oil refining wastewater, and then add 10ml of the prepared inoculant after centrifugation (5000 revolutions/min) to obtain the cultured bacteria and KH 2 PO 4 1.5mg, 3 parallel experiments, 30℃, 160 revolutions/min shaking reaction, and at the same time do not add sludge, do not add bacteria suspension, do not add KH 2 PO 4 The blank control and the control with only sludge. After 3 days, a sample was taken and centrifuged to determine the COD of the supernatant.

[0045] Category

[0046] It can be seen from the above table that the treatment effect of the sludge on oil refinery wastewater is 51.97% when no bacteria is added, and the degradation rate after addition of bacteria is 94.51%. After the bacteria is added to the sludge, the treatment efficiency is higher than that without the addition of bacteria. 42.52%

[0047] Example 2:

[0048] 1 The initial measurement of wastewater and the preparation of mixed bacterial liquid are the same as in Example 1.

[0049] 2 Add quantitative mixed bacterial liquid (containing medium) into the adsorbent to make bacterial agent, and add 1L medium to 1Kg adsorbent, and then pack and store at low temperature.

[0050]3 How to use: Add activated sludge to the SBR reactor at a 10% addition amount, add an inorganic salt medium containing 1000 mg/l glucose for sludge cultivation, use intermittent water changes, and change the water twice a day. After 7 days, the sedimentation rate of the sludge reaches 10% within 30 minutes, and then gradually increase the proportion of the refinery wastewater in the influent, so that the microorganisms in the sludge can be domesticated under the new living conditions that gradually adapt, until the influent is all the refinery wastewater. Then add the bacteria agent and KH of 1% of the total COD value of the refinery wastewater to the treatment system according to the 10% inoculum amount 2 PO 4 , At the same time, do a control experiment without adding bacteria. After 3 days, the COD value of influent and effluent is measured.

[0051] The results are as follows.

[0052] COD (mg/l) time (hour)

[0053] It can be seen from the above table that the treatment effect of the sludge on the refinery wastewater is 71.61% when no bacteria is added after 9 hours of treatment, and the degradation rate after addition of the bacteria is 89.79%. The efficiency of the treatment after the bacteria is added to the sludge is better than without the bacteria. The processing efficiency is increased by 25.39%.

[0054] Example 3:

[0055] Category

[0056] 2 Quantitatively prepare the solid medium after high temperature sterilization (121° C., 30 minutes). The formula of the solid medium is 0.3% beef extract, 1% peptone, 0.5% NaCl, 100 g water, and 1.7% agar.

[0057] 3 Pre-prepared strains and inoculate the pre-prepared 14357, EM, PNCB3, 10-4 into the sterilized solid medium for activation.

[0058] 4 Quantitatively prepare the liquid medium that has been sterilized by high temperature (121° C., 30 minutes). The liquid medium formula is 0.3% beef extract, 1% peptone, 0.5% NaCl, and 100 g of water.

[0059] 5 Inoculate the activated strains into the liquid medium and shake (37°C, 160 revolutions/min) for 3 days.

[0060] 6 Count the number of the cultured bacterial liquid, and then combine the number ratio of 14357:PNCB3:10-4:EM=2:1:10:3 to obtain a mixed bacterial liquid.

[0061] 7 Add the quantitative mixed bacterial liquid (containing medium) to the adsorbent, and add 0.5L medium to 1Kg of adsorbent, and then pack and store at low temperature.

[0062] COD (mg/l) time (hour)

[0063] It can be seen from the above table that the treatment effect of the sludge on the refinery wastewater after 9 hours of treatment is 66.71%, and the degradation rate after adding the bacteria agent reaches 86.73%. After the bacteria agent is added to the sludge, the treatment efficiency is 31.51 higher than the treatment efficiency without the bacteria agent. %.