Microbe bacteria agent for treatment of refinery waste water, its preparation method and application

A technology of microbial bacterial agent and oil refinery wastewater, which is applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., to achieve the effects of convenient use, improved treatment efficiency, and convenient transportation

Inactive Publication Date: 2006-06-21
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the shortcomings of priority control pollutants in my country's water such as soluble aliphatic hydrocarbons, benzene series, sulfur compounds and phthalates in the effluent of oil refinery wastewa

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example 1:

[0038] Category

[0039] 2 Quantitatively prepare the solid medium after high temperature sterilization (121°C, 30 minutes). The formula of the solid medium is beef extract 0.5%, peptone 1%, NaCl 0.5%, water 100g, and agar 1.7%.

[0040] 3 Pre-prepared strains and inoculate the pre-prepared 14357, Na4, EM, CB4, PNCB3, 10-4 into the sterilized solid medium for activation.

[0041] 4 Quantitatively prepare the liquid medium that has been sterilized by high temperature (121° C., 30 minutes). The liquid medium formula is beef extract 0.5%, peptone 1%, NaCl 0.5%, and water 100g.

[0042] 5 Inoculate the activated strains into the liquid medium and shake (37°C, 160 revolutions / min) for 2 days.

[0043] 6 Count the number of the cultured bacterial liquid, and then combine the number ratio of 14357:Na4:CB1:PNCB3:10-4:EM=3:4:2:3:3:6 to obtain a mixed bacterial liquid.

[0044] 8 How to use: Add 5ml of activated sludge to the Erlenmeyer flask containing 100ml o...

Example Embodiment

[0047] Example 2:

[0048] 1 The initial measurement of wastewater and the preparation of mixed bacterial liquid are the same as in Example 1.

[0049] 2 Add quantitative mixed bacterial liquid (containing medium) into the adsorbent to make bacterial agent, and add 1L medium to 1Kg adsorbent, and then pack and store at low temperature.

[0050]3 How to use: Add activated sludge to the SBR reactor at a 10% addition amount, add an inorganic salt medium containing 1000 mg / l glucose for sludge cultivation, use intermittent water changes, and change the water twice a day. After 7 days, the sedimentation rate of the sludge reaches 10% within 30 minutes, and then gradually increase the proportion of the refinery wastewater in the influent, so that the microorganisms in the sludge can be domesticated under the new living conditions that gradually adapt, until the influent is all the refinery wastewater. Then add the bacteria agent and KH of 1% of the total COD value of the refinery wastew...

Example Embodiment

[0054] Example 3:

[0055] Category

[0056] 2 Quantitatively prepare the solid medium after high temperature sterilization (121° C., 30 minutes). The formula of the solid medium is 0.3% beef extract, 1% peptone, 0.5% NaCl, 100 g water, and 1.7% agar.

[0057] 3 Pre-prepared strains and inoculate the pre-prepared 14357, EM, PNCB3, 10-4 into the sterilized solid medium for activation.

[0058] 4 Quantitatively prepare the liquid medium that has been sterilized by high temperature (121° C., 30 minutes). The liquid medium formula is 0.3% beef extract, 1% peptone, 0.5% NaCl, and 100 g of water.

[0059] 5 Inoculate the activated strains into the liquid medium and shake (37°C, 160 revolutions / min) for 3 days.

[0060] 6 Count the number of the cultured bacterial liquid, and then combine the number ratio of 14357:PNCB3:10-4:EM=2:1:10:3 to obtain a mixed bacterial liquid.

[0061] 7 Add the quantitative mixed bacterial liquid (containing medium) to the adsorbent, and add 0.5L...

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Abstract

The invention discloses a microbiological bacterium for processing waste water in oil refining, its preparing process and use. The bacterium comprises Rhodococcus, Microbacterium spp, bacillus, Candida and Brachybacterium by the ratio of 0-10:0-10:0-10:0-10:0-10:0-10. The bacterium can be activated for treating waste water.

Description

Technical field [0001] The invention belongs to the field of environmental protection, and relates to a microbial inoculum, its preparation method and its application, and specifically to the use of various refractory compounds such as alkane, aromatic hydrocarbon, substituted benzene, phenol, etc. according to the principles of microbial ecology. The symbiosis, mutual growth, and synergy between high-efficiency degrading bacteria are constructed into a microbial inoculum for treating oil refinery wastewater, and a preparation method and use method of the inoculum. Background technique [0002] Refinery wastewater is a high-concentration, refractory organic wastewater with a large amount of discharge. It mainly contains pollutants such as alkanes, aromatic hydrocarbons, volatile phenols, and ammonia nitrogen. Due to the extremely toxic compounds of alkanes, aromatic hydrocarbons, and volatile phenols, the discharge contains these pollutants. Wastewater wit...

Claims

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Application Information

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IPC IPC(8): C12N1/00C02F3/34C12N1/14C12N1/20
Inventor 李旭东朱永光廖银章杨俊仕
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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