Bacillus strain for degrading aflatoxin B1 and screening and application thereof

Abstract

The invention discloses a bacillus strain for degrading aflatoxin B1 and screening and application thereof. The class of the strain is named bacillus aryabhattai DT and perserved in the China GeneralMicrobiological Culture Collection Center, with a perservation number of CGMCC 14949. A treatment method comprises: strain screening and separation by using a medium taking coumarin as a unique carbonsource, and 16S rDNA authentication. Meanwhile, the degradation effect of the fermentation culture solution of the strain on aflatoxin B1 is explored. A single colony of bacillus aryabhattai on a solid medium is picked and inoculated in 50mL of growth medium and then vibration is performed for 12-24h on a shaking table at 30-40 DEG C; the fermentation culture solution of the bacillus aryabhattaireacts with 2mu g / mL aflatoxin B1 for 3-4d in a dark place to realize a degradation rate of 82.98%. The screened bacillus aryabhattai realizes an obvious degradation effect on aflatoxin B1 and has thecharacteristics of high efficiency, energy saving, environmental friendliness and the like, and can be applied to the preparation of relevant aflatoxin detoxification agents.

Description

technical field [0001] The invention belongs to the field of new strain screening technology and microorganism technology, and particularly relates to a bacillus strain capable of degrading aflatoxin B1 and application thereof. Background technique [0002] Aflatoxin is the most toxic mycotoxin found so far, and its basic structure is difuran ring and coumarin. It is mainly a secondary heterocyclic metabolite produced by Aspergillus flavus and Aspergillus parasiticus. Aflatoxins are highly toxic substances with strong carcinogenicity. Long-term intake of toxins accumulate in the liver can cause liver cancer and cause cancer in other organs such as kidneys, lungs, and gastrointestinal tract. Among all aflatoxins, aflatoxins B1 (AFB1) is the most toxic and has been classified as a Class I human carcinogen by the International Agency for Research on Cancer (IARC). Aflatoxins are mainly found in moldy peanuts, grains, nuts and rice. According to the statistics of the Food and...

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Problems solved by technology

Physical methods mainly include light, ultrasonic and ultra-high temperature, etc., but these processes may be affected by factors such as temperature and time, resulting in damage and loss of nutrients in food

The chemical method is to add some substances to degrade or destroy aflatoxins, such as adding oxidants, ammonium solution and sodium hydroxide method, etc., but these chemical substances may remain in food and are difficult to remove, so conventional physical and chemical methods to remove Aflatoxins in food have disadvantages such as unstable effects, large loss of nutrients, poor palatability of feed, and difficulty in large-scale production, so it is difficult to be widely used in production practice

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